Restriction map for
pFN21AE5621
(Circular) (Six-base) MAPSORT of: pFN21AE5621.seq Check: 1379 from: 1 to: 6241
pFN21AE5621 6241 bp
With 182 enzymes: SgfI *
December 8, 2014 13:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4051 278
Size: 53 83 186 936 2515 2468
Fragments arranged by size:
2515 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3584 1937
Size: 1647 4594
Acc65I G'GTAC_C
Cuts at: 3562 3562
Size: 6241
AclI AA'CG_TT
Cuts at: 1563 4368 4741 1563
Size: 2805 373 3063
Fragments arranged by size:
3063 2805 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2516 4304 5688 5956 1632
Size: 884 1788 1384 268 1917
Fragments arranged by size:
1917 1788 1384 884 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6044
AflIII A'CryG_T
Cuts at: 1203 2586 5140 6117 1203
Size: 1383 2554 977 1327
Fragments arranged by size:
2554 1383 1327 977
AgeI A'CCGG_T
Cuts at: 5072 5072
Size: 6241
AhdI GACnn_n'nnGTC
Cuts at: 4970 4970
Size: 6241
AleI CACnn'nnGTG
Cuts at: 1166 2228 2558 1166
Size: 1062 330 4849
Fragments arranged by size:
4849 1062 330
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2633 1184
Size: 1449 4792
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2724 2756 2724
Size: 32 6209
AlwNI CAG_nnn'CTG
Cuts at: 3038 3984 5556 3038
Size: 946 1572 3723
Fragments arranged by size:
3723 1572 946
ApaLI G'TGCA_C
Cuts at: 4298 5454 4298
Size: 1156 5085
ApoI r'AATT_y
Cuts at: 1493 3173 3550 3776 3928 6037 1493
Size: 1680 377 226 152 2109 1697
Fragments arranged by size:
2109 1697 1680 377 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4795 160
Size: 872 3763 1606
Fragments arranged by size:
3763 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6241
AvaI C'yCGr_G
Cuts at: 1870 1943 3566 1870
Size: 73 1623 4545
Fragments arranged by size:
4545 1623 73
AvrII C'CTAG_G
Cuts at: 3043 3043
Size: 6241
BamHI G'GATC_C
Cuts at: 3571 3938 3571
Size: 367 5874
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3562 5017 618
Size: 325 217 627 1775 1455 1842
Fragments arranged by size:
1842 1775 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2159 2186 3123 3354 3560 1391
Size: 768 27 937 231 206 4072
Fragments arranged by size:
4072 937 768 231 206 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2251 928
Size: 971 352 4918
Fragments arranged by size:
4918 971 352
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4432 4466 1903
Size: 34 2495 34 3678
Fragments arranged by size:
3678 2495 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3344 4133 5343 3344
Size: 789 1210 4242
Fragments arranged by size:
4242 1210 789
BclI T'GATC_A
Cuts at: 1548 3375 1548
Size: 1827 4414
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4852 136
Size: 107 122 71 631 605 3180 1525
Fragments arranged by size:
3180 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2058 6236 2058
Size: 4178 2063
BlpI GC'TnA_GC
Cuts at: 3658 3658
Size: 6241
Bme1580I G_kGCm'C
Cuts at: 1790 3501 4302 5458 1790
Size: 1711 801 1156 2573
Fragments arranged by size:
2573 1711 1156 801
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6241
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2019 3972 4930
Size: 416 53 83 399 24 602 1953 958
Cuts at: 4930 442
Size: 1753
Fragments arranged by size:
1953 1753 958 602 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6241
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2287 2426 2486 2675 2784 4901 1239
Size: 1048 139 60 189 109 2117 2579
Fragments arranged by size:
2579 2117 1048 189 139 109 60
Bpu10I CC'TnA_GC
Cuts at: 3012 3012
Size: 6241
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1997 3185 4366 5231 5529 1997
Size: 1188 1181 865 298 2709
Fragments arranged by size:
2709 1188 1181 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2933 4904 882
Size: 617 1434 1971 2219
Fragments arranged by size:
2219 1971 1434 617
BsaAI yAC'GTr
Cuts at: 493 1204 4032 493
Size: 711 2828 2702
Fragments arranged by size:
2828 2702 711
BsaBI GATnn'nnATC
Cuts at: 6235 6235
Size: 6241
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4048 4430 5065 275
Size: 53 83 186 936 2515 382 635 1451
Fragments arranged by size:
2515 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2295 4674 5072 5346 5493 5904 2295
Size: 2379 398 274 147 411 2632
Fragments arranged by size:
2632 2379 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2192 2222 2724 2754 1178
Size: 30 165 30 789 30 502 30 4665
Fragments arranged by size:
4665 789 502 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3425 3542 1186
Size: 2239 117 3885
Fragments arranged by size:
3885 2239 117
BseYI C'CCAG_C
Cuts at: 2263 2333 2782 3140 5444 5940 2263
Size: 70 449 358 2304 496 2564
Fragments arranged by size:
2564 2304 496 449 358 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2459 3051 1163
Size: 573 723 592 4353
Fragments arranged by size:
4353 723 592 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3691 4452 5480 1540
Size: 275 186 1690 761 1028 2301
Fragments arranged by size:
2301 1690 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2186 3123 3354 4302 4387 5458 2186
Size: 937 231 948 85 1071 2969
Fragments arranged by size:
2969 1071 948 937 231 85
BsmI GAATG_Cn'
Cuts at: 3752 3845 3752
Size: 93 6148
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2159 2186 3123 3354 3501 3560 4302
Size: 399 369 27 937 231 147 59 742
Cuts at: 4302 4387 5458 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 937 742 399 369 231 147
85 59 27
BspEI T'CCGG_A
Cuts at: 5904 5904
Size: 6241
BspHI T'CATG_A
Cuts at: 4130 5860 4130
Size: 1730 4511
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2373 2697 2856 3405 3596 844
Size: 1529 324 159 549 191 3489
Fragments arranged by size:
3489 1529 549 324 191 159
BsrBI CCG'CTC
Cuts at: 1394 1823 4128 1394
Size: 429 2305 3507
Fragments arranged by size:
3507 2305 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4736 4910 65
Size: 1183 3488 174 1396
Fragments arranged by size:
3488 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4885 5072 1700
Size: 110 3075 187 2869
Fragments arranged by size:
3075 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6241
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6241
BssSI C'ACGA_G
Cuts at: 4301 5313 4301
Size: 1012 5229
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3038 794
Size: 2244 3997
BstBI TT'CG_AA
Cuts at: 5045 5045
Size: 6241
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6241
BstYI r'GATC_y
Cuts at: 1972 2058 2370 2745 3135 3571 3938 4323 4340
Size: 86 312 375 390 436 367 385 17
Cuts at: 4340 5792 5878 5890 6236 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 436 390 385 375 367 346
312 86 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2083 2625 2858 3126 513
Size: 552 939 79 542 233 268 3628
Fragments arranged by size:
3628 939 552 542 268 233 79
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5502
Fragments arranged by size:
5502 683 56
BtsI GCAGTG_nn'
Cuts at: 2148 3757 4551 4571 2148
Size: 1609 794 20 3818
Fragments arranged by size:
3818 1609 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6241
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6206
DraI TTT'AAA
Cuts at: 3545 3545
Size: 6241
DraIII CAC_nnn'GTG
Cuts at: 2482 3490 2482
Size: 1008 5233
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5248 809
Size: 507 3932 1802
Fragments arranged by size:
3932 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2539 2820 3688
Size: 54 1425 50 275 18 709 281 868
Cuts at: 3688 4577 6206 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 868 709 281 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3688 1537
Size: 275 1876 4090
Fragments arranged by size:
4090 1876 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4171 1353
Size: 2818 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2081 3071 4828 5202 5348 387
Size: 1064 138 492 990 1757 374 146 1280
Fragments arranged by size:
1757 1280 1064 990 492 374 146 138
EcoICRI GAG'CTC
Cuts at: 2184 3121 3352 2184
Size: 937 231 5073
Fragments arranged by size:
5073 937 231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2287 2426 2486 2516 2675 2784 4304
Size: 393 655 139 60 30 159 109 1520
Cuts at: 4304 4901 5688 5956 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1520 787 655 597 393 268 159
139 109 60 30
EcoNI CCTnn'n_nnAGG
Cuts at: 3042 3042
Size: 6241
EcoO109I rG'GnC_Cy
Cuts at: 2410 3519 2410
Size: 1109 5132
EcoRI G'AATT_C
Cuts at: 3550 3928 3550
Size: 378 5863
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2446 2446
Size: 6241
FspI TGC'GCA
Cuts at: 4747 4747
Size: 6241
HaeII r_GCGC'y
Cuts at: 1678 5388 1678
Size: 3710 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2180 2212 2737 2769 2858
Size: 32 135 32 608 32 525 32 89
Cuts at: 2858 2890 4882 4914 4956 4988 1373
Size: 32 1992 32 42 32 2626
Fragments arranged by size:
2626 1992 608 525 135 89 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2362 3585 3831 669
Size: 988 281 424 1223 246 3079
Fragments arranged by size:
3079 1223 988 424 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6241
HpaI GTT'AAC
Cuts at: 3831 3831
Size: 6241
KpnI G_GTAC'C
Cuts at: 3566 3566
Size: 6241
MfeI C'AATT_G
Cuts at: 3252 3840 3252
Size: 588 5653
MluI A'CGCG_T
Cuts at: 6117 6117
Size: 6241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5355 5539 1305
Size: 4050 184 2007
Fragments arranged by size:
4050 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2822 10
Size: 54 1425 343 990 3429
Fragments arranged by size:
3429 1425 990 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2102 2228 2558 4199 4558 4717 518
Size: 648 936 126 330 1641 359 159 2042
Fragments arranged by size:
2042 1641 936 648 359 330 159 126
MspA1I CmG'CkG
Cuts at: 1606 2116 2267 3657 4334 5482 5727 1606
Size: 510 151 1390 677 1148 245 2120
Fragments arranged by size:
2120 1390 1148 677 510 245 151
NcoI C'CATG_G
Cuts at: 513 1065 2004 2083 3126 513
Size: 552 939 79 1043 3628
Fragments arranged by size:
3628 1043 939 552 79
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6241
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6241
NotI GC'GGCC_GC
Cuts at: 3688 3688
Size: 6241
NspI r_CATG'y
Cuts at: 1132 1229 2222 2464 2590 2986 3599 5144 1132
Size: 97 993 242 126 396 613 1545 2229
Fragments arranged by size:
2229 1545 993 613 396 242 126 97
PasI CC'CwG_GG
Cuts at: 1348 3514 1348
Size: 2166 4075
PciI A'CATG_T
Cuts at: 2586 5140 2586
Size: 2554 3687
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2782 1108
Size: 1674 4567
PfoI T'CCnGG_A
Cuts at: 2373 2373
Size: 6241
PmeI GTTT'AAAC
Cuts at: 3545 3545
Size: 6241
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2724 2756 4310 4342 5823 5855 2724
Size: 32 1554 32 1481 32 3110
Fragments arranged by size:
3110 1554 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3519 3519
Size: 6241
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6241
PsiI TTA'TAA
Cuts at: 3811 3811
Size: 6241
PstI C_TGCA'G
Cuts at: 830 2370 3086 3507 3593 830
Size: 1540 716 421 86 3478
Fragments arranged by size:
3478 1540 716 421 86
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6241
PvuII CAG'CTG
Cuts at: 2267 2267
Size: 6241
SacI G_AGCT'C
Cuts at: 2186 3123 3354 2186
Size: 937 231 5073
Fragments arranged by size:
5073 937 231
SalI G'TCGA_C
Cuts at: 1936 3583 1936
Size: 1647 4594
SbfI CC_TGCA'GG
Cuts at: 3593 3593
Size: 6241
ScaI AGT'ACT
Cuts at: 3445 4489 6201 3445
Size: 1044 1712 3485
Fragments arranged by size:
3485 1712 1044
SfcI C'TryA_G
Cuts at: 826 1045 2366 2923 3082 3503 3589 4724 5405
Size: 219 1321 557 159 421 86 1135 681
Cuts at: 5405 5596 826
Size: 191 1471
Fragments arranged by size:
1471 1321 1135 681 557 421 219 191
159 86
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6241
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6241
SmaI CCC'GGG
Cuts at: 1872 3568 1872
Size: 1696 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2012 3164 4345 5246 5508 820
Size: 197 926 69 1152 1181 901 262 1553
Fragments arranged by size:
1553 1181 1152 926 901 262 197 69
SnaBI TAC'GTA
Cuts at: 493 4032 493
Size: 3539 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6241
SphI G_CATG'C
Cuts at: 1132 2464 3599 1132
Size: 1332 1135 3774
Fragments arranged by size:
3774 1332 1135
SspI AAT'ATT
Cuts at: 5 52 4165 5
Size: 47 4113 2081
Fragments arranged by size:
4113 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6241
StyI C'CwwG_G
Cuts at: 513 1065 2004 2083 3043 3126 3522 3680 513
Size: 552 939 79 960 83 396 158 3074
Fragments arranged by size:
3074 960 939 552 396 158 83 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4268 4285 4438 4623 5068 325
Size: 1255 2688 17 153 185 445 1498
Fragments arranged by size:
2688 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3443 4487 6199
Size: 274 80 33 51 1443 1466 1044 1712
Cuts at: 6199 96
Size: 138
Fragments arranged by size:
1712 1466 1443 1044 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2757 2789 2920 2952 6201 6233 2757
Size: 32 131 32 3249 32 2765
Fragments arranged by size:
3249 2765 131 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6241
XbaI T'CTAG_A
Cuts at: 3577 3577
Size: 6241
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6241
XmaI C'CCGG_G
Cuts at: 1870 3566 1870
Size: 1696 4545
XmnI GAAnn'nnTTC
Cuts at: 4370 4370
Size: 6241
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4049 276
Size: 53 83 186 936 2515 2468
Fragments arranged by size:
2515 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI