Restriction map for
pFN21AE5618
(Circular) (Six-base) MAPSORT of: pFN21AE5618.seq Check: 1672 from: 1 to: 6304
pFN21AE5618 6304 bp
With 182 enzymes: SgfI *
December 8, 2014 13:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3059 3059
Size: 6304
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2015 4114 278
Size: 53 83 186 936 479 2099 2468
Fragments arranged by size:
2468 2099 936 479 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3647 1937
Size: 1710 4594
Acc65I G'GTAC_C
Cuts at: 3004 3625 3004
Size: 621 5683
AclI AA'CG_TT
Cuts at: 1563 4431 4804 1563
Size: 2868 373 3063
Fragments arranged by size:
3063 2868 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2142 2295 3531 4367 5751 6019 1632
Size: 510 153 1236 836 1384 268 1917
Fragments arranged by size:
1917 1384 1236 836 510 268 153
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6107
AflIII A'CryG_T
Cuts at: 1203 3593 5203 6180 1203
Size: 2390 1610 977 1327
Fragments arranged by size:
2390 1610 1327 977
AgeI A'CCGG_T
Cuts at: 5135 5135
Size: 6304
AhdI GACnn_n'nnGTC
Cuts at: 5033 5033
Size: 6304
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6304
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6304
AlwNI CAG_nnn'CTG
Cuts at: 2255 4047 5619 2255
Size: 1792 1572 2940
Fragments arranged by size:
2940 1792 1572
ApaLI G'TGCA_C
Cuts at: 3072 4361 5517 3072
Size: 1289 1156 3859
Fragments arranged by size:
3859 1289 1156
ApoI r'AATT_y
Cuts at: 1493 2213 2649 2848 3036 3613 3839 3991 6100
Size: 720 436 199 188 577 226 152 2109
Cuts at: 6100 1493
Size: 1697
Fragments arranged by size:
2109 1697 720 577 436 226 199 188
152
AseI AT'TA_AT
Cuts at: 160 1032 3080 4858 160
Size: 872 2048 1778 1606
Fragments arranged by size:
2048 1778 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6304
AvaI C'yCGr_G
Cuts at: 1870 1943 3393 3629 1870
Size: 73 1450 236 4545
Fragments arranged by size:
4545 1450 236 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3327 3360 3327
Size: 33 6271
BamHI G'GATC_C
Cuts at: 3634 4001 3634
Size: 367 5937
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3004 3625 5080 618
Size: 325 217 627 1217 621 1455 1842
Fragments arranged by size:
1842 1455 1217 627 621 325 217
BanII G_rGCy'C
Cuts at: 1391 3623 1391
Size: 2232 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5333
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1999 2033 4495 4529 1903
Size: 34 62 34 2462 34 3678
Fragments arranged by size:
3678 2462 62 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2287 4196 5406 2287
Size: 1909 1210 3185
Fragments arranged by size:
3185 1909 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6304
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4915 136
Size: 107 122 71 631 605 3243 1525
Fragments arranged by size:
3243 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2470 6299 2470
Size: 3829 2475
BlpI GC'TnA_GC
Cuts at: 3721 3721
Size: 6304
Bme1580I G_kGCm'C
Cuts at: 1790 2922 3076 4365 5521 1790
Size: 1132 154 1289 1156 2573
Fragments arranged by size:
2573 1289 1156 1132 154
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6304
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4035 4993 442
Size: 416 53 83 399 24 2618 958 1753
Fragments arranged by size:
2618 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6304
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2193 2854 4964 1239
Size: 954 661 2110 2579
Fragments arranged by size:
2579 2110 954 661
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2176 4429 5294 5592 2176
Size: 2253 865 298 2888
Fragments arranged by size:
2888 2253 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2400 2943 4967 882
Size: 617 901 543 2024 2219
Fragments arranged by size:
2219 2024 901 617 543
BsaAI yAC'GTr
Cuts at: 493 1204 3526 4095 493
Size: 711 2322 569 2702
Fragments arranged by size:
2702 2322 711 569
BsaBI GATnn'nnATC
Cuts at: 6298 6298
Size: 6304
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 4111 4493 5128
Size: 53 83 186 936 479 2099 382 635
Cuts at: 5128 275
Size: 1451
Fragments arranged by size:
2099 1451 936 635 479 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4737 5135 5409 5556 5967 4737
Size: 398 274 147 411 5074
Fragments arranged by size:
5074 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2959 2989 1178
Size: 30 165 30 1556 30 4493
Fragments arranged by size:
4493 1556 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3378 3381 1186
Size: 2192 3 4109
Fragments arranged by size:
4109 2192 3
BseYI C'CCAG_C
Cuts at: 2370 3211 5507 6003 2370
Size: 841 2296 496 2671
Fragments arranged by size:
2671 2296 841 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3426 1163
Size: 573 1690 4041
Fragments arranged by size:
4041 1690 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3754 4515 5543 1540
Size: 275 186 1753 761 1028 2301
Fragments arranged by size:
2301 1753 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3076 3310 4365 4450 5521 3076
Size: 234 1055 85 1071 3859
Fragments arranged by size:
3859 1071 1055 234 85
BsmI GAATG_Cn'
Cuts at: 3815 3908 3815
Size: 93 6211
BsmBI CGTCTCn'nnnn_
Cuts at: 2019 2019
Size: 6304
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2922 3076 3310 3623 4365 4450 5521
Size: 399 1132 154 234 313 742 85 1071
Cuts at: 5521 1391
Size: 2174
Fragments arranged by size:
2174 1132 1071 742 399 313 234 154
85
BspEI T'CCGG_A
Cuts at: 5967 5967
Size: 6304
BspHI T'CATG_A
Cuts at: 4193 5923 4193
Size: 1730 4574
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2159 2992 3059 3659 844
Size: 1315 833 67 600 3489
Fragments arranged by size:
3489 1315 833 600 67
BsrBI CCG'CTC
Cuts at: 1394 1823 4191 1394
Size: 429 2368 3507
Fragments arranged by size:
3507 2368 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3003 4799 4973 65
Size: 1183 1755 1796 174 1396
Fragments arranged by size:
1796 1755 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4948 5135 1700
Size: 110 3138 187 2869
Fragments arranged by size:
3138 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2096 96
Size: 2000 4304
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6304
BssSI C'ACGA_G
Cuts at: 4364 5376 4364
Size: 1012 5292
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6304
BstBI TT'CG_AA
Cuts at: 5108 5108
Size: 6304
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6304
BstYI r'GATC_y
Cuts at: 1972 2044 2470 3634 4001 4386 4403 5855 5941
Size: 72 426 1164 367 385 17 1452 86
Cuts at: 5941 5953 6299 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1164 426 385 367 346 86
72 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2061 2289 2613 3331 513
Size: 552 939 57 228 324 718 3486
Fragments arranged by size:
3486 939 718 552 324 228 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5565
Fragments arranged by size:
5565 683 56
BtsI GCAGTG_nn'
Cuts at: 2845 2949 3447 3820 4614 4634 2845
Size: 104 498 373 794 20 4515
Fragments arranged by size:
4515 794 498 373 104 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6304
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6269
DraI TTT'AAA
Cuts at: 3608 3608
Size: 6304
DraIII CAC_nnn'GTG
Cuts at: 3529 3529
Size: 6304
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5311 809
Size: 507 3995 1802
Fragments arranged by size:
3995 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2353 3379 3751
Size: 54 1425 50 275 18 523 1026 372
Cuts at: 3751 4640 6269 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1026 889 523 372 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3751 1537
Size: 275 1939 4090
Fragments arranged by size:
4090 1939 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2271 4234 1353
Size: 918 1963 3423
Fragments arranged by size:
3423 1963 918
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4891 5265 5411 387
Size: 1064 138 3302 374 146 1280
Fragments arranged by size:
3302 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2142 2193 2295 2854 3531 4367 4964
Size: 393 510 51 102 559 677 836 597
Cuts at: 4964 5751 6019 1239
Size: 787 268 1524
Fragments arranged by size:
1524 836 787 677 597 559 510 393
268 102 51
EcoNI CCTnn'n_nnAGG
Cuts at: 2155 2403 2155
Size: 248 6056
EcoO109I rG'GnC_Cy
Cuts at: 2027 2027
Size: 6304
EcoRI G'AATT_C
Cuts at: 2213 2649 3613 3991 2213
Size: 436 964 378 4526
Fragments arranged by size:
4526 964 436 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2147 2147
Size: 6304
FspI TGC'GCA
Cuts at: 4810 4810
Size: 6304
HaeII r_GCGC'y
Cuts at: 1678 5451 1678
Size: 3773 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2762 2794 2915 2947 3162
Size: 32 135 32 1190 32 121 32 215
Cuts at: 3162 3194 3376 3408 3416 3448 4945 4977 5019
Size: 32 182 32 8 32 1497 32 42
Cuts at: 5019 5051 1373
Size: 32 2626
Fragments arranged by size:
2626 1497 1190 215 182 135 121 42
32 32 32 32 32 32 32 32
32 8
HincII GTy'rAC
Cuts at: 669 1657 1938 2271 3236 3648 3894 669
Size: 988 281 333 965 412 246 3079
Fragments arranged by size:
3079 988 965 412 333 281 246
HindIII A'AGCT_T
Cuts at: 748 2567 748
Size: 1819 4485
HpaI GTT'AAC
Cuts at: 3894 3894
Size: 6304
KpnI G_GTAC'C
Cuts at: 3008 3629 3008
Size: 621 5683
MfeI C'AATT_G
Cuts at: 2109 3903 2109
Size: 1794 4510
MluI A'CGCG_T
Cuts at: 6180 6180
Size: 6304
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2333 3122 3265 5418 5602 1305
Size: 1028 789 143 2153 184 2007
Fragments arranged by size:
2153 2007 1028 789 184 143
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3381 10
Size: 54 1425 343 1549 2933
Fragments arranged by size:
2933 1549 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4262 4621 4780 518
Size: 648 3096 359 159 2042
Fragments arranged by size:
3096 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2041 3720 4397 5545 5790 1606
Size: 435 1679 677 1148 245 2120
Fragments arranged by size:
2120 1679 1148 677 435 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2289 2613 3331 513
Size: 552 939 285 324 718 3486
Fragments arranged by size:
3486 939 718 552 324 285
NdeI CA'TA_TG
Cuts at: 387 2136 387
Size: 1749 4555
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6304
NotI GC'GGCC_GC
Cuts at: 3751 3751
Size: 6304
NspI r_CATG'y
Cuts at: 1132 1229 3597 3662 5207 1132
Size: 97 2368 65 1545 2229
Fragments arranged by size:
2368 2229 1545 97 65
PasI CC'CwG_GG
Cuts at: 1348 2915 1348
Size: 1567 4737
PciI A'CATG_T
Cuts at: 3593 5203 3593
Size: 1610 4694
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3503 3529 1108
Size: 2395 26 3883
Fragments arranged by size:
3883 2395 26
PmeI GTTT'AAAC
Cuts at: 3608 3608
Size: 6304
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4373 4405 5886 5918 4373
Size: 32 1481 32 4759
Fragments arranged by size:
4759 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2027 2027
Size: 6304
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6304
PsiI TTA'TAA
Cuts at: 3874 3874
Size: 6304
PspXI vC'TCGA_Gb
Cuts at: 3393 3393
Size: 6304
PstI C_TGCA'G
Cuts at: 830 2806 3243 3656 830
Size: 1976 437 413 3478
Fragments arranged by size:
3478 1976 437 413
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6304
PvuII CAG'CTG
Cuts at: 2041 2041
Size: 6304
SalI G'TCGA_C
Cuts at: 1936 3646 1936
Size: 1710 4594
SanDI GG'GwC_CC
Cuts at: 2027 2027
Size: 6304
SbfI CC_TGCA'GG
Cuts at: 3656 3656
Size: 6304
ScaI AGT'ACT
Cuts at: 4552 6264 4552
Size: 1712 4592
SfcI C'TryA_G
Cuts at: 826 1045 2256 2802 3239 3652 4787 5468 5659
Size: 219 1211 546 437 413 1135 681 191
Cuts at: 5659 826
Size: 1471
Fragments arranged by size:
1471 1211 1135 681 546 437 413 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6304
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6304
SmaI CCC'GGG
Cuts at: 1872 3631 1872
Size: 1759 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2155 3393 4408 5309 5571 820
Size: 197 926 212 1238 1015 901 262 1553
Fragments arranged by size:
1553 1238 1015 926 901 262 212 197
SnaBI TAC'GTA
Cuts at: 493 4095 493
Size: 3602 2702
SpeI A'CTAG_T
Cuts at: 152 3092 3586 152
Size: 2940 494 2870
Fragments arranged by size:
2940 2870 494
SphI G_CATG'C
Cuts at: 1132 3662 1132
Size: 2530 3774
SspI AAT'ATT
Cuts at: 5 52 4228 5
Size: 47 4176 2081
Fragments arranged by size:
4176 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6304
StyI C'CwwG_G
Cuts at: 513 1065 2004 2289 2613 2978 3331 3743 513
Size: 552 939 285 324 365 353 412 3074
Fragments arranged by size:
3074 939 552 412 365 353 324 285
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4331 4348 4501 4686 5131 325
Size: 1255 2751 17 153 185 445 1498
Fragments arranged by size:
2751 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2096 4550 6262
Size: 274 80 33 51 1443 119 2454 1712
Cuts at: 6262 96
Size: 138
Fragments arranged by size:
2454 1712 1443 274 138 119 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2974 3006 6264 6296 2974
Size: 32 3258 32 2982
Fragments arranged by size:
3258 2982 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3247 1316
Size: 1931 4373
XbaI T'CTAG_A
Cuts at: 3640 3640
Size: 6304
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2038 2038
Size: 6304
XhoI C'TCGA_G
Cuts at: 1943 3393 1943
Size: 1450 4854
XmaI C'CCGG_G
Cuts at: 1870 3629 1870
Size: 1759 4545
XmnI GAAnn'nnTTC
Cuts at: 2848 4433 2848
Size: 1585 4719
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2013 4112 276
Size: 53 83 186 936 479 2099 2468
Fragments arranged by size:
2468 2099 936 479 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI PvuII SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PsrI RsrII SacI SacII
SapI SexAI SfiI SfoI SrfI SwaI