Restriction map for
pFN21AE5614
(Circular) (Six-base) MAPSORT of: pFN21AE5614.seq Check: 1240 from: 1 to: 6298
pFN21AE5614 6298 bp
With 182 enzymes: SgfI *
December 8, 2014 13:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2763 4108 278
Size: 53 83 186 936 1227 1345 2468
Fragments arranged by size:
2468 1345 1227 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3641 1937
Size: 1704 4594
Acc65I G'GTAC_C
Cuts at: 3330 3619 3330
Size: 289 6009
AclI AA'CG_TT
Cuts at: 1563 4425 4798 1563
Size: 2862 373 3063
Fragments arranged by size:
3063 2862 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2001 2522 2618 2991 3263 4361 5745 6013
Size: 369 521 96 373 272 1098 1384 268
Cuts at: 6013 1632
Size: 1917
Fragments arranged by size:
1917 1384 1098 521 373 369 272 268
96
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6101
AflIII A'CryG_T
Cuts at: 1203 3324 5197 6174 1203
Size: 2121 1873 977 1327
Fragments arranged by size:
2121 1873 1327 977
AgeI A'CCGG_T
Cuts at: 5129 5129
Size: 6298
AhdI GACnn_n'nnGTC
Cuts at: 5027 5027
Size: 6298
AleI CACnn'nnGTG
Cuts at: 1166 3530 1166
Size: 2364 3934
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6298
AlwNI CAG_nnn'CTG
Cuts at: 3174 4041 5613 3174
Size: 867 1572 3859
Fragments arranged by size:
3859 1572 867
ApaLI G'TGCA_C
Cuts at: 2585 2860 4355 5511 2585
Size: 275 1495 1156 3372
Fragments arranged by size:
3372 1495 1156 275
ApoI r'AATT_y
Cuts at: 1493 2395 3207 3607 3833 3985 6094 1493
Size: 902 812 400 226 152 2109 1697
Fragments arranged by size:
2109 1697 902 812 400 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4852 160
Size: 872 3820 1606
Fragments arranged by size:
3820 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6298
AvaI C'yCGr_G
Cuts at: 1870 1943 3163 3623 1870
Size: 73 1220 460 4545
Fragments arranged by size:
4545 1220 460 73
BamHI G'GATC_C
Cuts at: 3628 3995 3628
Size: 367 5931
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2174 3330 3619 5074 618
Size: 325 217 627 387 1156 289 1455 1842
Fragments arranged by size:
1842 1455 1156 627 387 325 289 217
BanII G_rGCy'C
Cuts at: 1391 3617 1391
Size: 2226 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5327
BbvCI CC'TCA_GC
Cuts at: 3305 3305
Size: 6298
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4489 4523 1903
Size: 34 2552 34 3678
Fragments arranged by size:
3678 2552 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2869 3484 4190 5400 2869
Size: 615 706 1210 3767
Fragments arranged by size:
3767 1210 706 615
BclI T'GATC_A
Cuts at: 1548 2041 1548
Size: 493 5805
BfrBI ATG'CAT
Cuts at: 2128 2170 2458 2128
Size: 42 288 5968
Fragments arranged by size:
5968 288 42
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2934 4909 136
Size: 107 122 71 631 605 1262 1975 1525
Fragments arranged by size:
1975 1525 1262 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6293 6293
Size: 6298
BlpI GC'TnA_GC
Cuts at: 3715 3715
Size: 6298
Bme1580I G_kGCm'C
Cuts at: 1790 2179 2589 2864 3537 4359 5515 1790
Size: 389 410 275 673 822 1156 2573
Fragments arranged by size:
2573 1156 822 673 410 389 275
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6298
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2426 2849 4029
Size: 416 53 83 399 24 1009 423 1180
Cuts at: 4029 4987 442
Size: 958 1753
Fragments arranged by size:
1753 1180 1009 958 423 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6298
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2175 2277 2866 3099 3532 4958 1239
Size: 936 102 589 233 433 1426 2579
Fragments arranged by size:
2579 1426 936 589 433 233 102
Bpu10I CC'TnA_GC
Cuts at: 3131 3305 3131
Size: 174 6124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3197 4423 5288 5586 3197
Size: 1226 865 298 3909
Fragments arranged by size:
3909 1226 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3098 4961 882
Size: 617 1599 1863 2219
Fragments arranged by size:
2219 1863 1599 617
BsaAI yAC'GTr
Cuts at: 493 1204 4089 493
Size: 711 2885 2702
Fragments arranged by size:
2885 2702 711
BsaBI GATnn'nnATC
Cuts at: 2549 6292 2549
Size: 3743 2555
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2760 4105 4487 5122
Size: 53 83 186 936 1227 1345 382 635
Cuts at: 5122 275
Size: 1451
Fragments arranged by size:
1451 1345 1227 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4731 5129 5403 5550 5961 4731
Size: 398 274 147 411 5068
Fragments arranged by size:
5068 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2585 2615 2879 2909 3078
Size: 30 165 30 1182 30 264 30 169
Cuts at: 3078 3108 3293 3323 1178
Size: 30 185 30 4153
Fragments arranged by size:
4153 1182 264 185 169 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6298
BseYI C'CCAG_C
Cuts at: 5501 5997 5501
Size: 496 5802
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5725
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3748 4509 5537 1540
Size: 275 186 1747 761 1028 2301
Fragments arranged by size:
2301 1747 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2589 2864 4359 4444 5515 2589
Size: 275 1495 85 1071 3372
Fragments arranged by size:
3372 1495 1071 275 85
BsmI GAATG_Cn'
Cuts at: 2128 2417 2485 3135 3809 3902 2128
Size: 289 68 650 674 93 4524
Fragments arranged by size:
4524 674 650 289 93 68
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2179 2589 2864 3537 3617 4359 4444
Size: 399 389 410 275 673 80 742 85
Cuts at: 4444 5515 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 673 410 399 389 275
85 80
BspEI T'CCGG_A
Cuts at: 5961 5961
Size: 6298
BspHI T'CATG_A
Cuts at: 4187 5917 4187
Size: 1730 4568
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3653 844
Size: 2809 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4185 1394
Size: 429 2362 3507
Fragments arranged by size:
3507 2362 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2172 3429 4793 4967 65
Size: 1183 924 1257 1364 174 1396
Fragments arranged by size:
1396 1364 1257 1183 924 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4942 5129 1700
Size: 110 3132 187 2869
Fragments arranged by size:
3132 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6298
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6298
BssSI C'ACGA_G
Cuts at: 4358 5370 4358
Size: 1012 5286
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3049 3174 3586 794
Size: 2255 125 412 3506
Fragments arranged by size:
3506 2255 412 125
BstBI TT'CG_AA
Cuts at: 5102 5102
Size: 6298
BstEII G'GTnAC_C
Cuts at: 1184 2985 1184
Size: 1801 4497
BstYI r'GATC_y
Cuts at: 1972 3014 3628 3995 4380 4397 5849 5935 5947
Size: 1042 614 367 385 17 1452 86 12
Cuts at: 5947 6293 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1042 614 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2332 3150 2332
Size: 818 5480
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5746
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2473 487
Size: 683 56 1247 4312
Fragments arranged by size:
4312 1247 683 56
BtsI GCAGTG_nn'
Cuts at: 2009 2422 3814 4608 4628 2009
Size: 413 1392 794 20 3679
Fragments arranged by size:
3679 1392 794 413 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6298
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6263
DraI TTT'AAA
Cuts at: 3602 3602
Size: 6298
DraIII CAC_nnn'GTG
Cuts at: 2584 2584
Size: 6298
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5305 809
Size: 507 3989 1802
Fragments arranged by size:
3989 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2628 2935 3745
Size: 54 1425 50 275 18 798 307 810
Cuts at: 3745 4634 6263 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 810 798 307 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3745 1537
Size: 275 1933 4090
Fragments arranged by size:
4090 1933 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2053 2763 4228 1353
Size: 700 710 1465 3423
Fragments arranged by size:
3423 1465 710 700
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4885 5259 5405 387
Size: 1064 138 3296 374 146 1280
Fragments arranged by size:
3296 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2001 2175 2277 2522 2618 2866 2991
Size: 393 369 174 102 245 96 248 125
Cuts at: 2991 3099 3263 3532 4361 4958 5745 6013 1239
Size: 108 164 269 829 597 787 268 1524
Fragments arranged by size:
1524 829 787 597 393 369 269 268
248 245 174 164 125 108 102 96
EcoO109I rG'GnC_Cy
Cuts at: 2328 3217 3507 2328
Size: 889 290 5119
Fragments arranged by size:
5119 889 290
EcoRI G'AATT_C
Cuts at: 3607 3985 3607
Size: 378 5920
EcoRV GAT'ATC
Cuts at: 2547 2671 2547
Size: 124 6174
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3022 3022
Size: 6298
FspI TGC'GCA
Cuts at: 4804 4804
Size: 6298
HaeII r_GCGC'y
Cuts at: 1678 5445 1678
Size: 3767 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2585 2617 3078 3110 3293
Size: 32 135 32 1013 32 461 32 183
Cuts at: 3293 3325 4939 4971 5013 5045 1373
Size: 32 1614 32 42 32 2626
Fragments arranged by size:
2626 1614 1013 461 183 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2269 2560 3364 3642 3888 669
Size: 988 281 331 291 804 278 246 3079
Fragments arranged by size:
3079 988 804 331 291 281 278 246
HindIII A'AGCT_T
Cuts at: 748 3004 748
Size: 2256 4042
HpaI GTT'AAC
Cuts at: 2269 3888 2269
Size: 1619 4679
KpnI G_GTAC'C
Cuts at: 3334 3623 3334
Size: 289 6009
MfeI C'AATT_G
Cuts at: 3897 3897
Size: 6298
MluI A'CGCG_T
Cuts at: 6174 6174
Size: 6298
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5412 5596 1305
Size: 4107 184 2007
Fragments arranged by size:
4107 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2630 2937 10
Size: 54 1425 343 798 307 3371
Fragments arranged by size:
3371 1425 798 343 307 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3530 4256 4615 4774 518
Size: 648 2364 726 359 159 2042
Fragments arranged by size:
2364 2042 726 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3714 4391 5539 5784 1606
Size: 2108 677 1148 245 2120
Fragments arranged by size:
2120 2108 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5746
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6298
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6298
NotI GC'GGCC_GC
Cuts at: 3745 3745
Size: 6298
NsiI A_TGCA'T
Cuts at: 2130 2172 2460 2130
Size: 42 288 5968
Fragments arranged by size:
5968 288 42
NspI r_CATG'y
Cuts at: 1132 1229 2128 2320 2458 2462 3328 3656 5201
Size: 97 899 192 138 4 866 328 1545
Cuts at: 5201 1132
Size: 2229
Fragments arranged by size:
2229 1545 899 866 328 192 138 97
4
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6298
PciI A'CATG_T
Cuts at: 3324 5197 3324
Size: 1873 4425
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6298
PfoI T'CCnGG_A
Cuts at: 2901 2901
Size: 6298
PmeI GTTT'AAAC
Cuts at: 3602 3602
Size: 6298
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4367 4399 5880 5912 4367
Size: 32 1481 32 4753
Fragments arranged by size:
4753 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2328 3217 3507 2328
Size: 889 290 5119
Fragments arranged by size:
5119 889 290
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6298
PsiI TTA'TAA
Cuts at: 3868 3868
Size: 6298
PstI C_TGCA'G
Cuts at: 830 2797 3054 3650 830
Size: 1967 257 596 3478
Fragments arranged by size:
3478 1967 596 257
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6298
SalI G'TCGA_C
Cuts at: 1936 3640 1936
Size: 1704 4594
SanDI GG'GwC_CC
Cuts at: 3507 3507
Size: 6298
SapI GCTCTTCn'nnn_
Cuts at: 2053 2053
Size: 6298
SbfI CC_TGCA'GG
Cuts at: 3650 3650
Size: 6298
ScaI AGT'ACT
Cuts at: 4546 6258 4546
Size: 1712 4586
SfcI C'TryA_G
Cuts at: 826 1045 2781 2793 3050 3646 4781 5462 5653
Size: 219 1736 12 257 596 1135 681 191
Cuts at: 5653 826
Size: 1471
Fragments arranged by size:
1736 1471 1135 681 596 257 219 191
12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6298
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6298
SmaI CCC'GGG
Cuts at: 1872 3625 1872
Size: 1753 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3212 4402 5303 5565 820
Size: 197 926 1269 1190 901 262 1553
Fragments arranged by size:
1553 1269 1190 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4089 493
Size: 3596 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6298
SphI G_CATG'C
Cuts at: 1132 2128 2462 3656 1132
Size: 996 334 1194 3774
Fragments arranged by size:
3774 1194 996 334
SspI AAT'ATT
Cuts at: 5 52 4222 5
Size: 47 4170 2081
Fragments arranged by size:
4170 2081 47
StuI AGG'CCT
Cuts at: 1517 3119 1517
Size: 1602 4696
StyI C'CwwG_G
Cuts at: 513 1065 3502 3737 513
Size: 552 2437 235 3074
Fragments arranged by size:
3074 2437 552 235
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2962 4325 4342 4495 4680 5125 325
Size: 1255 1382 1363 17 153 185 445 1498
Fragments arranged by size:
1498 1382 1363 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4544 6256 96
Size: 274 80 33 51 1443 2567 1712 138
Fragments arranged by size:
2567 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3499 3531 6258 6290 3499
Size: 32 2727 32 3507
Fragments arranged by size:
3507 2727 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3090 1316
Size: 1774 4524
XbaI T'CTAG_A
Cuts at: 3634 3634
Size: 6298
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2470 2470
Size: 6298
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6298
XmaI C'CCGG_G
Cuts at: 1870 3623 1870
Size: 1753 4545
XmnI GAAnn'nnTTC
Cuts at: 4427 4427
Size: 6298
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2761 4106 276
Size: 53 83 186 936 1227 1345 2468
Fragments arranged by size:
2468 1345 1227 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SexAI
SfiI SfoI SrfI SwaI