Restriction map for
pFN21AE5613
(Circular) (Six-base) MAPSORT of: pFN21AE5613.seq Check: 693 from: 1 to: 6160
pFN21AE5613 6160 bp
With 182 enzymes: SgfI *
December 8, 2014 13:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2960 2960
Size: 6160
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3970 278
Size: 53 83 186 936 2434 2468
Fragments arranged by size:
2468 2434 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3503 1937
Size: 1566 4594
Acc65I G'GTAC_C
Cuts at: 3481 3481
Size: 6160
AclI AA'CG_TT
Cuts at: 1563 4287 4660 1563
Size: 2724 373 3063
Fragments arranged by size:
3063 2724 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2710 3449 4223 5607 5875 1632
Size: 1078 739 774 1384 268 1917
Fragments arranged by size:
1917 1384 1078 774 739 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5963
AflIII A'CryG_T
Cuts at: 1203 5059 6036 1203
Size: 3856 977 1327
Fragments arranged by size:
3856 1327 977
AgeI A'CCGG_T
Cuts at: 4991 4991
Size: 6160
AhdI GACnn_n'nnGTC
Cuts at: 4889 4889
Size: 6160
AleI CACnn'nnGTG
Cuts at: 1166 2930 1166
Size: 1764 4396
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6160
AlwNI CAG_nnn'CTG
Cuts at: 2053 3903 5475 2053
Size: 1850 1572 2738
Fragments arranged by size:
2738 1850 1572
ApaI G_GGCC'C
Cuts at: 2091 2091
Size: 6160
ApaLI G'TGCA_C
Cuts at: 2491 4217 5373 2491
Size: 1726 1156 3278
Fragments arranged by size:
3278 1726 1156
ApoI r'AATT_y
Cuts at: 1493 3469 3695 3847 5956 1493
Size: 1976 226 152 2109 1697
Fragments arranged by size:
2109 1976 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2030 2030
Size: 6160
AseI AT'TA_AT
Cuts at: 160 1032 4714 160
Size: 872 3682 1606
Fragments arranged by size:
3682 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6160
AvaI C'yCGr_G
Cuts at: 1870 1943 2090 2099 2788 3194 3445 3485 1870
Size: 73 147 9 689 406 251 40 4545
Fragments arranged by size:
4545 689 406 251 147 73 40 9
BamHI G'GATC_C
Cuts at: 3490 3857 3490
Size: 367 5793
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2166 2846 2921 2994 3481
Size: 325 217 627 379 680 75 73 487
Cuts at: 3481 4936 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 680 627 487 379 325 217
75 73
BanII G_rGCy'C
Cuts at: 1391 2091 2097 2226 3053 3479 1391
Size: 700 6 129 827 426 4072
Fragments arranged by size:
4072 827 700 426 129 6
BbeI G_GCGC'C
Cuts at: 2850 2850
Size: 6160
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2339 928
Size: 971 440 4749
Fragments arranged by size:
4749 971 440
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3313 3347 4351 4385 1903
Size: 34 1376 34 1004 34 3678
Fragments arranged by size:
3678 1376 1004 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4052 5262 4052
Size: 1210 4950
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6160
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2694 3380 4771
Size: 107 122 71 631 605 1022 686 1391
Cuts at: 4771 136
Size: 1525
Fragments arranged by size:
1525 1391 1022 686 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6155 6155
Size: 6160
BlpI GC'TnA_GC
Cuts at: 3577 3577
Size: 6160
Bme1580I G_kGCm'C
Cuts at: 1790 2091 2495 2924 2982 2997 3036 4221 5377
Size: 301 404 429 58 15 39 1185 1156
Cuts at: 5377 1790
Size: 2573
Fragments arranged by size:
2573 1185 1156 429 404 301 58 39
15
BmgBI CAC'GTC
Cuts at: 1318 2452 1318
Size: 1134 5026
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3891 4849 442
Size: 416 53 83 399 24 2474 958 1753
Fragments arranged by size:
2474 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2602 4820 1239
Size: 1363 2218 2579
Fragments arranged by size:
2579 2218 1363
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2171 4285 5150 5448 2171
Size: 2114 865 298 2883
Fragments arranged by size:
2883 2114 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4823 882
Size: 617 3324 2219
Fragments arranged by size:
3324 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3951 493
Size: 711 2747 2702
Fragments arranged by size:
2747 2702 711
BsaBI GATnn'nnATC
Cuts at: 6154 6154
Size: 6160
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2133 2705 2847 3967
Size: 53 83 186 936 600 572 142 1120
Cuts at: 3967 4349 4984 275
Size: 382 635 1451
Fragments arranged by size:
1451 1120 936 635 600 572 382 186
142 83 53
BsaWI w'CCGG_w
Cuts at: 2403 3086 4593 4991 5265 5412 5823 2403
Size: 683 1507 398 274 147 411 2740
Fragments arranged by size:
2740 1507 683 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5935
Fragments arranged by size:
5935 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2459 2539 2605 1186
Size: 1273 80 66 4741
Fragments arranged by size:
4741 1273 80 66
BseYI C'CCAG_C
Cuts at: 2692 2917 3040 3153 5363 5859 2692
Size: 225 123 113 2210 496 2993
Fragments arranged by size:
2993 2210 496 225 123 113
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2306 2885 1163
Size: 573 570 579 4438
Fragments arranged by size:
4438 579 573 570
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2212 3610 4371 5399 1540
Size: 275 186 211 1398 761 1028 2301
Fragments arranged by size:
2301 1398 1028 761 275 211 186
BsiHKAI G_wGCw'C
Cuts at: 2226 2235 2495 2502 4221 4306 5377 2226
Size: 9 260 7 1719 85 1071 3009
Fragments arranged by size:
3009 1719 1071 260 85 9 7
BsmI GAATG_Cn'
Cuts at: 3671 3764 3671
Size: 93 6067
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2097 2226 2235 2495 2502 2924
Size: 399 301 6 129 9 260 7 422
Cuts at: 2924 2982 2997 3036 3053 3479 4221 4306 5377
Size: 58 15 39 17 426 742 85 1071
Cuts at: 5377 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 426 422 399 301 260
129 85 58 39 17 15 9 7
6
BspEI T'CCGG_A
Cuts at: 3086 5823 3086
Size: 2737 3423
BspHI T'CATG_A
Cuts at: 4049 5779 4049
Size: 1730 4430
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2960 3515 844
Size: 2116 555 3489
Fragments arranged by size:
3489 2116 555
BsrBI CCG'CTC
Cuts at: 1394 1823 2194 2656 4047 1394
Size: 429 371 462 1391 3507
Fragments arranged by size:
3507 1391 462 429 371
BsrDI GCAATG_nn'
Cuts at: 65 1248 2717 4655 4829 65
Size: 1183 1469 1938 174 1396
Fragments arranged by size:
1938 1469 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2034 4804 4991 1700
Size: 110 224 2770 187 2869
Fragments arranged by size:
2869 2770 224 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6160
BssHII G'CGCG_C
Cuts at: 1925 2030 1925
Size: 105 6055
BssSI C'ACGA_G
Cuts at: 2324 2942 4220 5232 2324
Size: 618 1278 1012 3252
Fragments arranged by size:
3252 1278 1012 618
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2053 794
Size: 1259 4901
BstBI TT'CG_AA
Cuts at: 4964 4964
Size: 6160
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6160
BstXI CCAn_nnnn'nTGG
Cuts at: 2626 3236 2626
Size: 610 5550
BstYI r'GATC_y
Cuts at: 1972 2157 3490 3857 4242 4259 5711 5797 5809
Size: 185 1333 367 385 17 1452 86 12
Cuts at: 5809 6155 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1333 385 367 346 185 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2060 2073 2389 3120 513
Size: 552 939 56 13 316 731 3553
Fragments arranged by size:
3553 939 731 552 316 56 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2866 3121 487
Size: 683 56 1640 255 3526
Fragments arranged by size:
3526 1640 683 255 56
BtsI GCAGTG_nn'
Cuts at: 3068 3676 4470 4490 3068
Size: 608 794 20 4738
Fragments arranged by size:
4738 794 608 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6160
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6125
DraI TTT'AAA
Cuts at: 3464 3464
Size: 6160
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5167 809
Size: 507 3851 1802
Fragments arranged by size:
3851 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2209 2354 2571
Size: 54 1425 50 275 18 379 145 217
Cuts at: 2571 3060 3123 3357 3607 4496 6125 8
Size: 489 63 234 250 889 1629 43
Fragments arranged by size:
1629 1425 889 489 379 275 250 234
217 145 63 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2209 3607 1537
Size: 275 397 1398 4090
Fragments arranged by size:
4090 1398 397 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2150 2201 3404 4090 1353
Size: 797 51 1203 686 3423
Fragments arranged by size:
3423 1203 797 686 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2287 4747 5121 5267 387
Size: 1064 138 698 2460 374 146 1280
Fragments arranged by size:
2460 1280 1064 698 374 146 138
EcoICRI GAG'CTC
Cuts at: 2224 2224
Size: 6160
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2602 2710 3449 4223 4820 5607 5875
Size: 393 970 108 739 774 597 787 268
Cuts at: 5875 1239
Size: 1524
Fragments arranged by size:
1524 970 787 774 739 597 393 268
108
EcoNI CCTnn'n_nnAGG
Cuts at: 3227 3227
Size: 6160
EcoO109I rG'GnC_Cy
Cuts at: 2125 2182 2507 3191 2125
Size: 57 325 684 5094
Fragments arranged by size:
5094 684 325 57
EcoRI G'AATT_C
Cuts at: 3469 3847 3469
Size: 378 5782
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2212 2212
Size: 6160
FspI TGC'GCA
Cuts at: 4666 4666
Size: 6160
HaeII r_GCGC'y
Cuts at: 1678 2850 3366 5307 1678
Size: 1172 516 1941 2531
Fragments arranged by size:
2531 1941 1172 516
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3058 3090 4801 4833 4875
Size: 32 135 32 1486 32 1711 32 42
Cuts at: 4875 4907 1373
Size: 32 2626
Fragments arranged by size:
2626 1711 1486 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3504 3750 669
Size: 988 281 1566 246 3079
Fragments arranged by size:
3079 1566 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6160
HpaI GTT'AAC
Cuts at: 3750 3750
Size: 6160
KasI G'GCGC_C
Cuts at: 2846 2846
Size: 6160
KpnI G_GTAC'C
Cuts at: 3485 3485
Size: 6160
MfeI C'AATT_G
Cuts at: 3759 3759
Size: 6160
MluI A'CGCG_T
Cuts at: 6036 6036
Size: 6160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2421 5274 5458 1305
Size: 1116 2853 184 2007
Fragments arranged by size:
2853 2007 1116 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4338
Fragments arranged by size:
4338 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2252 2930 3107 4118 4477 4636 518
Size: 648 1086 678 177 1011 359 159 2042
Fragments arranged by size:
2042 1086 1011 678 648 359 177 159
MspA1I CmG'CkG
Cuts at: 1606 2062 2085 2391 2692 2914 2930 3122 3427
Size: 456 23 306 301 222 16 192 305
Cuts at: 3427 3576 4253 5401 5646 1606
Size: 149 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 456 306 305 301 245
222 192 149 23 16
NaeI GCC'GGC
Cuts at: 2036 2036
Size: 6160
NarI GG'CG_CC
Cuts at: 2847 2847
Size: 6160
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4669
Fragments arranged by size:
4669 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6160
NgoMIV G'CCGG_C
Cuts at: 2034 2034
Size: 6160
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6160
NotI GC'GGCC_GC
Cuts at: 3607 3607
Size: 6160
NspI r_CATG'y
Cuts at: 1132 1229 2674 3518 5063 1132
Size: 97 1445 844 1545 2229
Fragments arranged by size:
2229 1545 1445 844 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6160
PciI A'CATG_T
Cuts at: 5059 5059
Size: 6160
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6160
PmeI GTTT'AAAC
Cuts at: 3464 3464
Size: 6160
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4229 4261 5742 5774 4229
Size: 32 1481 32 4615
Fragments arranged by size:
4615 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2125 2182 3191 2125
Size: 57 1009 5094
Fragments arranged by size:
5094 1009 57
PshAI GACnn'nnGTC
Cuts at: 1613 2774 1613
Size: 1161 4999
PsiI TTA'TAA
Cuts at: 3730 3730
Size: 6160
PspOMI G'GGCC_C
Cuts at: 2087 2087
Size: 6160
PstI C_TGCA'G
Cuts at: 830 2058 3174 3512 830
Size: 1228 1116 338 3478
Fragments arranged by size:
3478 1228 1116 338
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6160
PvuII CAG'CTG
Cuts at: 2692 3427 2692
Size: 735 5425
SacI G_AGCT'C
Cuts at: 2226 2226
Size: 6160
SacII CC_GC'GG
Cuts at: 2063 2392 3123 2063
Size: 329 731 5100
Fragments arranged by size:
5100 731 329
SalI G'TCGA_C
Cuts at: 1936 3502 1936
Size: 1566 4594
SapI GCTCTTCn'nnn_
Cuts at: 2201 2201
Size: 6160
SbfI CC_TGCA'GG
Cuts at: 3512 3512
Size: 6160
ScaI AGT'ACT
Cuts at: 4408 6120 4408
Size: 1712 4448
SfcI C'TryA_G
Cuts at: 826 1045 2054 2734 3170 3508 4643 5324 5515
Size: 219 1009 680 436 338 1135 681 191
Cuts at: 5515 826
Size: 1471
Fragments arranged by size:
1471 1135 1009 681 680 436 338 219
191
SfoI GGC'GCC
Cuts at: 2848 2848
Size: 6160
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6160
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6160
SmaI CCC'GGG
Cuts at: 1872 2101 3447 3487 1872
Size: 229 1346 40 4545
Fragments arranged by size:
4545 1346 229 40
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2186 4264 5165 5427 820
Size: 197 926 243 2078 901 262 1553
Fragments arranged by size:
2078 1553 926 901 262 243 197
SnaBI TAC'GTA
Cuts at: 493 3951 493
Size: 3458 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6160
SphI G_CATG'C
Cuts at: 1132 2674 3518 1132
Size: 1542 844 3774
Fragments arranged by size:
3774 1542 844
SspI AAT'ATT
Cuts at: 5 52 4084 5
Size: 47 4032 2081
Fragments arranged by size:
4032 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6160
StyI C'CwwG_G
Cuts at: 513 1065 2004 3291 3599 513
Size: 552 939 1287 308 3074
Fragments arranged by size:
3074 1287 939 552 308
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2893 4187 4204 4357 4542 4987 325
Size: 1255 1313 1294 17 153 185 445 1498
Fragments arranged by size:
1498 1313 1294 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3099 4406 6118
Size: 274 80 33 51 1443 1122 1307 1712
Cuts at: 6118 96
Size: 138
Fragments arranged by size:
1712 1443 1307 1122 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6120 6152 6120
Size: 32 6128
Tth111I GACn'n_nGTC
Cuts at: 1316 2707 1316
Size: 1391 4769
XbaI T'CTAG_A
Cuts at: 3496 3496
Size: 6160
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2585 3298 2585
Size: 713 5447
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6160
XmaI C'CCGG_G
Cuts at: 1870 2099 3445 3485 1870
Size: 229 1346 40 4545
Fragments arranged by size:
4545 1346 229 40
XmnI GAAnn'nnTTC
Cuts at: 2411 3392 4289 2411
Size: 981 897 4282
Fragments arranged by size:
4282 981 897
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3968 276
Size: 53 83 186 936 2434 2468
Fragments arranged by size:
2468 2434 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI
NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII
SanDI SexAI SfiI SrfI SwaI