Restriction map for
pFN21AE5610
(Circular) (Six-base) MAPSORT of: pFN21AE5610.seq Check: 840 from: 1 to: 6457
pFN21AE5610 6457 bp
With 182 enzymes: SgfI *
December 8, 2014 13:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4267 278
Size: 53 83 186 936 2731 2468
Fragments arranged by size:
2731 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3800 1937
Size: 1863 4594
Acc65I G'GTAC_C
Cuts at: 3778 3778
Size: 6457
AclI AA'CG_TT
Cuts at: 1563 4584 4957 1563
Size: 3021 373 3063
Fragments arranged by size:
3063 3021 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2507 3560 3620 4520 5904 6172 1632
Size: 875 1053 60 900 1384 268 1917
Fragments arranged by size:
1917 1384 1053 900 875 268 60
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6260
AflIII A'CryG_T
Cuts at: 1203 2892 5356 6333 1203
Size: 1689 2464 977 1327
Fragments arranged by size:
2464 1689 1327 977
AgeI A'CCGG_T
Cuts at: 5288 5288
Size: 6457
AhdI GACnn_n'nnGTC
Cuts at: 5186 5186
Size: 6457
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6457
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3197 1184
Size: 2013 4444
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2747 2779 2747
Size: 32 6425
AlwNI CAG_nnn'CTG
Cuts at: 2189 3566 4200 5772 2189
Size: 1377 634 1572 2874
Fragments arranged by size:
2874 1572 1377 634
ApaLI G'TGCA_C
Cuts at: 4514 5670 4514
Size: 1156 5301
ApoI r'AATT_y
Cuts at: 1493 3766 3992 4144 6253 1493
Size: 2273 226 152 2109 1697
Fragments arranged by size:
2273 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5011 160
Size: 872 3979 1606
Fragments arranged by size:
3979 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6457
AvaI C'yCGr_G
Cuts at: 1870 1943 2312 3052 3077 3782 1870
Size: 73 369 740 25 705 4545
Fragments arranged by size:
4545 740 705 369 73 25
BamHI G'GATC_C
Cuts at: 3310 3787 4154 3310
Size: 477 367 5613
Fragments arranged by size:
5613 477 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3778 5233 618
Size: 325 217 627 1991 1455 1842
Fragments arranged by size:
1991 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2148 2431 3084 3447 3776 1391
Size: 757 283 653 363 329 4072
Fragments arranged by size:
4072 757 653 363 329 283
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2712 2739 3374 928
Size: 971 813 27 635 4011
Fragments arranged by size:
4011 971 813 635 27
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2384 2418 4648 4682 1903
Size: 34 447 34 2230 34 3678
Fragments arranged by size:
3678 2230 447 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2253 4349 5559 2253
Size: 2096 1210 3151
Fragments arranged by size:
3151 2096 1210
BclI T'GATC_A
Cuts at: 1548 2110 1548
Size: 562 5895
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3620 5068 136
Size: 107 122 71 631 605 1948 1448 1525
Fragments arranged by size:
1948 1525 1448 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2593 6452 2593
Size: 3859 2598
BlpI GC'TnA_GC
Cuts at: 3008 3874 3008
Size: 866 5591
Bme1580I G_kGCm'C
Cuts at: 1790 4518 5674 1790
Size: 2728 1156 2573
Fragments arranged by size:
2728 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2478 1318
Size: 1160 5297
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4188 5146 442
Size: 416 53 83 399 24 2771 958 1753
Fragments arranged by size:
2771 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3597 1056
Size: 2541 3916
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2249 2684 3123 3242 5117 1239
Size: 1010 435 439 119 1875 2579
Fragments arranged by size:
2579 1875 1010 439 435 119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4582 5447 5745 4582
Size: 865 298 5294
Fragments arranged by size:
5294 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5120 882
Size: 617 3621 2219
Fragments arranged by size:
3621 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2197 2702 4248 493
Size: 711 993 505 1546 2702
Fragments arranged by size:
2702 1546 993 711 505
BsaBI GATnn'nnATC
Cuts at: 6451 6451
Size: 6457
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4264 4646 5281 275
Size: 53 83 186 936 2731 382 635 1451
Fragments arranged by size:
2731 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3525 4890 5288 5562 5709 6120 3525
Size: 1365 398 274 147 411 3862
Fragments arranged by size:
3862 1365 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2747 2777 2882 2912 3454
Size: 30 165 30 1344 30 105 30 542
Cuts at: 3454 3484 1178
Size: 30 4151
Fragments arranged by size:
4151 1344 542 165 105 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2156 2690 1186
Size: 970 534 4953
Fragments arranged by size:
4953 970 534
BseYI C'CCAG_C
Cuts at: 3166 5660 6156 3166
Size: 2494 496 3467
Fragments arranged by size:
3467 2494 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3662 1163
Size: 573 1926 3958
Fragments arranged by size:
3958 1926 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3246 3907 4668 5696 1540
Size: 275 186 1245 661 761 1028 2301
Fragments arranged by size:
2301 1245 1028 761 661 275 186
BsiHKAI G_wGCw'C
Cuts at: 2148 2313 2511 4518 4603 5674 2148
Size: 165 198 2007 85 1071 2931
Fragments arranged by size:
2931 2007 1071 198 165 85
BsmI GAATG_Cn'
Cuts at: 3968 4061 3968
Size: 93 6364
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2148 2313 2431 2511 3084 3447 3776
Size: 399 358 165 118 80 573 363 329
Cuts at: 3776 4518 4603 5674 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 573 399 363 358 329
165 118 85 80
BspEI T'CCGG_A
Cuts at: 3525 6120 3525
Size: 2595 3862
BspHI T'CATG_A
Cuts at: 3360 4346 6076 3360
Size: 986 1730 3741
Fragments arranged by size:
3741 1730 986
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3812 844
Size: 2968 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4344 1394
Size: 429 2521 3507
Fragments arranged by size:
3507 2521 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2565 2819 4952 5126 65
Size: 1183 1317 254 2133 174 1396
Fragments arranged by size:
2133 1396 1317 1183 254 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5101 5288 1700
Size: 110 3291 187 2869
Fragments arranged by size:
3291 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2253 96
Size: 2157 4300
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6457
BssSI C'ACGA_G
Cuts at: 4517 5529 4517
Size: 1012 5445
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2974 794
Size: 2180 4277
BstBI TT'CG_AA
Cuts at: 5261 5261
Size: 6457
BstEII G'GTnAC_C
Cuts at: 1184 2207 3514 1184
Size: 1023 1307 4127
Fragments arranged by size:
4127 1307 1023
BstXI CCAn_nnnn'nTGG
Cuts at: 2413 3197 3397 2413
Size: 784 200 5473
Fragments arranged by size:
5473 784 200
BstYI r'GATC_y
Cuts at: 1972 2593 3310 3645 3787 4154 4539 4556 6008
Size: 621 717 335 142 367 385 17 1452
Cuts at: 6008 6094 6106 6452 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 717 621 385 367 346 335
142 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2379 2379
Size: 6457
BtgI C'CryG_G
Cuts at: 513 1065 3371 3519 513
Size: 552 2306 148 3451
Fragments arranged by size:
3451 2306 552 148
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3598 487
Size: 683 56 2372 3346
Fragments arranged by size:
3346 2372 683 56
BtsI GCAGTG_nn'
Cuts at: 2190 3235 3973 4767 4787 2190
Size: 1045 738 794 20 3860
Fragments arranged by size:
3860 1045 794 738 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6457
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6422
DraI TTT'AAA
Cuts at: 3761 3761
Size: 6457
DraIII CAC_nnn'GTG
Cuts at: 2262 2262
Size: 6457
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5464 809
Size: 507 4148 1802
Fragments arranged by size:
4148 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 3199 3904
Size: 54 1425 50 275 18 210 1159 705
Cuts at: 3904 4793 6422 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1159 889 705 275 210 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3904 1537
Size: 275 2092 4090
Fragments arranged by size:
4090 2092 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2598 2667 3191 3684 4387 1353
Size: 1245 69 524 493 703 3423
Fragments arranged by size:
3423 1245 703 524 493 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5044 5418 5564 387
Size: 1064 138 3455 374 146 1280
Fragments arranged by size:
3455 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2146 2146
Size: 6457
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2249 2507 2684 3123 3242 3560 3620
Size: 393 617 258 177 439 119 318 60
Cuts at: 3620 4520 5117 5904 6172 1239
Size: 900 597 787 268 1524
Fragments arranged by size:
1524 900 787 617 597 439 393 318
268 258 177 119 60
EcoO109I rG'GnC_Cy
Cuts at: 2749 3733 2749
Size: 984 5473
EcoRI G'AATT_C
Cuts at: 3766 4144 3766
Size: 378 6079
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3232 3446 3232
Size: 214 6243
FspI TGC'GCA
Cuts at: 4963 4963
Size: 6457
HaeII r_GCGC'y
Cuts at: 1678 5604 1678
Size: 3926 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2102 2134 2597 2629 3295
Size: 32 135 32 530 32 463 32 666
Cuts at: 3295 3327 3454 3486 3542 3574 5098 5130 5172
Size: 32 127 32 56 32 1524 32 42
Cuts at: 5172 5204 1373
Size: 32 2626
Fragments arranged by size:
2626 1524 666 530 463 135 127 56
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3801 4047 669
Size: 988 281 1863 246 3079
Fragments arranged by size:
3079 1863 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2066 3365 748
Size: 1318 1299 3840
Fragments arranged by size:
3840 1318 1299
HpaI GTT'AAC
Cuts at: 4047 4047
Size: 6457
KpnI G_GTAC'C
Cuts at: 3782 3782
Size: 6457
MfeI C'AATT_G
Cuts at: 3474 4056 3474
Size: 582 5875
MluI A'CGCG_T
Cuts at: 6333 6333
Size: 6457
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2034 2058 2270 5571 5755 1305
Size: 729 24 212 3301 184 2007
Fragments arranged by size:
3301 2007 729 212 184 24
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3201 10
Size: 54 1425 343 1369 3266
Fragments arranged by size:
3266 1425 1369 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4415 4774 4933 518
Size: 648 3249 359 159 2042
Fragments arranged by size:
3249 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2465 3873 4550 5698 5943 1606
Size: 859 1408 677 1148 245 2120
Fragments arranged by size:
2120 1408 1148 859 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3371 513
Size: 552 2306 3599
Fragments arranged by size:
3599 2306 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6457
NheI G'CTAG_C
Cuts at: 1052 3593 1052
Size: 2541 3916
NotI GC'GGCC_GC
Cuts at: 3904 3904
Size: 6457
NspI r_CATG'y
Cuts at: 1132 1229 2828 2896 3815 5360 1132
Size: 97 1599 68 919 1545 2229
Fragments arranged by size:
2229 1599 1545 919 97 68
PasI CC'CwG_GG
Cuts at: 1348 3617 1348
Size: 2269 4188
PciI A'CATG_T
Cuts at: 2892 5356 2892
Size: 2464 3993
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6457
PfoI T'CCnGG_A
Cuts at: 3105 3189 3433 3105
Size: 84 244 6129
Fragments arranged by size:
6129 244 84
PmeI GTTT'AAAC
Cuts at: 3761 3761
Size: 6457
PmlI CAC'GTG
Cuts at: 2702 2702
Size: 6457
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2747 2779 4526 4558 6039 6071 2747
Size: 32 1747 32 1481 32 3133
Fragments arranged by size:
3133 1747 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3521 1613
Size: 1908 4549
PsiI TTA'TAA
Cuts at: 4027 4027
Size: 6457
PstI C_TGCA'G
Cuts at: 830 2640 2970 3809 830
Size: 1810 330 839 3478
Fragments arranged by size:
3478 1810 839 330
PvuI CG_AT'CG
Cuts at: 2001 3246 2001
Size: 1245 5212
PvuII CAG'CTG
Cuts at: 2465 2465
Size: 6457
SacI G_AGCT'C
Cuts at: 2148 2148
Size: 6457
SalI G'TCGA_C
Cuts at: 1936 3799 1936
Size: 1863 4594
SapI GCTCTTCn'nnn_
Cuts at: 3191 3191
Size: 6457
SbfI CC_TGCA'GG
Cuts at: 2640 3809 2640
Size: 1169 5288
ScaI AGT'ACT
Cuts at: 4705 6417 4705
Size: 1712 4745
SexAI A'CCwGG_T
Cuts at: 3273 3389 3657 3273
Size: 116 268 6073
Fragments arranged by size:
6073 268 116
SfcI C'TryA_G
Cuts at: 826 1045 2433 2636 2966 3805 4940 5621 5812
Size: 219 1388 203 330 839 1135 681 191
Cuts at: 5812 826
Size: 1471
Fragments arranged by size:
1471 1388 1135 839 681 330 219 203
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6457
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6457
SmaI CCC'GGG
Cuts at: 1872 3784 1872
Size: 1912 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2312 4561 5462 5724 820
Size: 197 926 369 2249 901 262 1553
Fragments arranged by size:
2249 1553 926 901 369 262 197
SnaBI TAC'GTA
Cuts at: 493 4248 493
Size: 3755 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6457
SphI G_CATG'C
Cuts at: 1132 3815 1132
Size: 2683 3774
SspI AAT'ATT
Cuts at: 5 52 4381 5
Size: 47 4329 2081
Fragments arranged by size:
4329 2081 47
StuI AGG'CCT
Cuts at: 1517 2018 2484 3539 1517
Size: 501 466 1055 4435
Fragments arranged by size:
4435 1055 501 466
StyI C'CwwG_G
Cuts at: 513 1065 2013 2101 2881 3371 3624 3718 3896
Size: 552 948 88 780 490 253 94 178
Cuts at: 3896 513
Size: 3074
Fragments arranged by size:
3074 948 780 552 490 253 178 94
88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4484 4501 4654 4839 5284 325
Size: 1255 2904 17 153 185 445 1498
Fragments arranged by size:
2904 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2253 4703 6415
Size: 274 80 33 51 1443 276 2450 1712
Cuts at: 6415 96
Size: 138
Fragments arranged by size:
2450 1712 1443 276 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2516 2548 2882 2914 3592 3624 6417 6449 2516
Size: 32 334 32 678 32 2793 32 2524
Fragments arranged by size:
2793 2524 678 334 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6457
XbaI T'CTAG_A
Cuts at: 3724 3793 3724
Size: 69 6388
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2413 2572 2413
Size: 159 6298
XhoI C'TCGA_G
Cuts at: 1943 2312 1943
Size: 369 6088
XmaI C'CCGG_G
Cuts at: 1870 3782 1870
Size: 1912 4545
XmnI GAAnn'nnTTC
Cuts at: 4586 4586
Size: 6457
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4265 276
Size: 53 83 186 936 2731 2468
Fragments arranged by size:
2731 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SfiI SfoI SrfI SwaI