Restriction map for
pFN21AE5593
(Circular) (Six-base) MAPSORT of: pFN21AE5593.seq Check: 6466 from: 1 to: 5965
pFN21AE5593 5965 bp
With 182 enzymes: SgfI *
January 30, 2014 17:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3775 278
Size: 53 83 186 936 2239 2468
Fragments arranged by size:
2468 2239 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2854 3308 1937
Size: 917 454 4594
Fragments arranged by size:
4594 917 454
Acc65I G'GTAC_C
Cuts at: 3286 3286
Size: 5965
AclI AA'CG_TT
Cuts at: 1563 4092 4465 1563
Size: 2529 373 3063
Fragments arranged by size:
3063 2529 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2583 4028 5412 5680 1632
Size: 951 1445 1384 268 1917
Fragments arranged by size:
1917 1445 1384 951 268
AfeI AGC'GCT
Cuts at: 2070 2070
Size: 5965
AflII C'TTAA_G
Cuts at: 820 1017 2725 820
Size: 197 1708 4060
Fragments arranged by size:
4060 1708 197
AflIII A'CryG_T
Cuts at: 1203 2757 4864 5841 1203
Size: 1554 2107 977 1327
Fragments arranged by size:
2107 1554 1327 977
AgeI A'CCGG_T
Cuts at: 4796 4796
Size: 5965
AhdI GACnn_n'nnGTC
Cuts at: 4694 4694
Size: 5965
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5965
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5965
AlwNI CAG_nnn'CTG
Cuts at: 3708 5280 3708
Size: 1572 4393
ApaI G_GGCC'C
Cuts at: 2030 2030
Size: 5965
ApaLI G'TGCA_C
Cuts at: 2625 4022 5178 2625
Size: 1397 1156 3412
Fragments arranged by size:
3412 1397 1156
ApoI r'AATT_y
Cuts at: 1493 3274 3500 3652 5761 1493
Size: 1781 226 152 2109 1697
Fragments arranged by size:
2109 1781 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4519 160
Size: 872 3487 1606
Fragments arranged by size:
3487 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5965
AvaI C'yCGr_G
Cuts at: 1870 1943 2022 2491 3290 1870
Size: 73 79 469 799 4545
Fragments arranged by size:
4545 799 469 79 73
BamHI G'GATC_C
Cuts at: 3295 3662 3295
Size: 367 5598
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2137 2257 2330 2411 2715
Size: 325 217 627 350 120 73 81 304
Cuts at: 2715 2831 3286 4741 618
Size: 116 455 1455 1842
Fragments arranged by size:
1842 1455 627 455 350 325 304 217
120 116 81 73
BanII G_rGCy'C
Cuts at: 1391 2030 2640 3284 1391
Size: 639 610 644 4072
Fragments arranged by size:
4072 644 639 610
BbeI G_GCGC'C
Cuts at: 2141 2334 2415 2141
Size: 193 81 5691
Fragments arranged by size:
5691 193 81
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2091 3067 928
Size: 971 192 976 3826
Fragments arranged by size:
3826 976 971 192
BbvCI CC'TCA_GC
Cuts at: 2378 2450 2378
Size: 72 5893
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4156 4190 1903
Size: 34 50 34 2135 34 3678
Fragments arranged by size:
3678 2135 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3857 5067 3857
Size: 1210 4755
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5965
BfrBI ATG'CAT
Cuts at: 3160 3160
Size: 5965
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2615 4576 136
Size: 107 122 71 631 605 943 1961 1525
Fragments arranged by size:
1961 1525 943 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5960 5960
Size: 5965
BlpI GC'TnA_GC
Cuts at: 3382 3382
Size: 5965
Bme1580I G_kGCm'C
Cuts at: 1790 2030 2192 2262 2559 2629 4026 5182 1790
Size: 240 162 70 297 70 1397 1156 2573
Fragments arranged by size:
2573 1397 1156 297 240 162 70 70
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5965
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2514 3696 4654
Size: 416 53 83 399 24 1097 1182 958
Cuts at: 4654 442
Size: 1753
Fragments arranged by size:
1753 1182 1097 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5965
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2819 4625 1239
Size: 1580 1806 2579
Fragments arranged by size:
2579 1806 1580
Bpu10I CC'TnA_GC
Cuts at: 2378 2450 2378
Size: 72 5893
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2798 2915 4090 4955 5253 2798
Size: 117 1175 865 298 3510
Fragments arranged by size:
3510 1175 865 298 117
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2095 4628 882
Size: 617 596 2533 2219
Fragments arranged by size:
2533 2219 617 596
BsaAI yAC'GTr
Cuts at: 493 1204 2281 2758 3756 493
Size: 711 1077 477 998 2702
Fragments arranged by size:
2702 1077 998 711 477
BsaBI GATnn'nnATC
Cuts at: 5959 5959
Size: 5965
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2047 2138 2331 2412
Size: 53 83 186 936 514 91 193 81
Cuts at: 2412 3772 4154 4789 275
Size: 1360 382 635 1451
Fragments arranged by size:
1451 1360 936 635 514 382 193 186
91 83 81 53
BsaWI w'CCGG_w
Cuts at: 2580 2980 4398 4796 5070 5217 5628 2580
Size: 400 1418 398 274 147 411 2917
Fragments arranged by size:
2917 1418 411 400 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5740
Fragments arranged by size:
5740 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2083 2101 2119 2122 1186
Size: 897 18 18 3 5029
Fragments arranged by size:
5029 897 18 18 3
BseYI C'CCAG_C
Cuts at: 2085 2166 2429 2705 5168 5664 2085
Size: 81 263 276 2463 496 2386
Fragments arranged by size:
2463 2386 496 276 263 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2969 1163
Size: 573 1233 4159
Fragments arranged by size:
4159 1233 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2348 3415 4176 5204 1540
Size: 275 186 347 1067 761 1028 2301
Fragments arranged by size:
2301 1067 1028 761 347 275 186
BsiHKAI G_wGCw'C
Cuts at: 2021 2210 2322 2629 4026 4111 5182 2021
Size: 189 112 307 1397 85 1071 2804
Fragments arranged by size:
2804 1397 1071 307 189 112 85
BsmI GAATG_Cn'
Cuts at: 3476 3569 3476
Size: 93 5872
BsmBI CGTCTCn'nnnn_
Cuts at: 2515 2515
Size: 5965
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2030 2192 2210 2262 2322 2559
Size: 399 231 9 162 18 52 60 237
Cuts at: 2559 2629 2640 3284 4026 4111 5182 1391
Size: 70 11 644 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 644 399 237 231 162
85 70 60 52 18 11 9
BspEI T'CCGG_A
Cuts at: 2980 5628 2980
Size: 2648 3317
BspHI T'CATG_A
Cuts at: 3854 5584 3854
Size: 1730 4235
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2277 2685 3320 844
Size: 1433 408 635 3489
Fragments arranged by size:
3489 1433 635 408
BsrBI CCG'CTC
Cuts at: 1394 1823 2572 3852 1394
Size: 429 749 1280 3507
Fragments arranged by size:
3507 1280 749 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2506 4460 4634 65
Size: 1183 1258 1954 174 1396
Fragments arranged by size:
1954 1396 1258 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2300 2327 2343 2961 4609 4796 1700
Size: 110 490 27 16 618 1648 187 2869
Fragments arranged by size:
2869 1648 618 490 187 110 27 16
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5965
BssHII G'CGCG_C
Cuts at: 1925 2219 2404 1925
Size: 294 185 5486
Fragments arranged by size:
5486 294 185
BssSI C'ACGA_G
Cuts at: 4025 5037 4025
Size: 1012 4953
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5965
BstBI TT'CG_AA
Cuts at: 4769 4769
Size: 5965
BstEII G'GTnAC_C
Cuts at: 1184 2920 1184
Size: 1736 4229
BstYI r'GATC_y
Cuts at: 1972 3295 3662 4047 4064 5516 5602 5614 5960
Size: 1323 367 385 17 1452 86 12 346
Cuts at: 5960 1972
Size: 1977
Fragments arranged by size:
1977 1452 1323 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2711 2711
Size: 5965
BtgI C'CryG_G
Cuts at: 513 1065 2262 2681 513
Size: 552 1197 419 3797
Fragments arranged by size:
3797 1197 552 419
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5226
Fragments arranged by size:
5226 683 56
BtsI GCAGTG_nn'
Cuts at: 2627 3481 4275 4295 2627
Size: 854 794 20 4297
Fragments arranged by size:
4297 854 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5965
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5930
DraI TTT'AAA
Cuts at: 3269 3269
Size: 5965
DraIII CAC_nnn'GTG
Cuts at: 2554 2554
Size: 5965
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2758 4972 809
Size: 507 1442 2214 1802
Fragments arranged by size:
2214 1802 1442 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2313 2345 2364
Size: 54 1425 50 275 18 483 32 19
Cuts at: 2364 2466 2655 3412 4301 5930 8
Size: 102 189 757 889 1629 43
Fragments arranged by size:
1629 1425 889 757 483 275 189 102
54 50 43 32 19 18
EagI C'GGCC_G
Cuts at: 1537 1812 2345 3412 1537
Size: 275 533 1067 4090
Fragments arranged by size:
4090 1067 533 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2297 3895 1353
Size: 944 1598 3423
Fragments arranged by size:
3423 1598 944
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2143 2152 2372 2630 4552 4926
Size: 1064 138 554 9 220 258 1922 374
Cuts at: 4926 5072 387
Size: 146 1280
Fragments arranged by size:
1922 1280 1064 554 374 258 220 146
138 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2583 2819 4028 4625 5412 5680 1239
Size: 393 951 236 1209 597 787 268 1524
Fragments arranged by size:
1524 1209 951 787 597 393 268 236
EcoNI CCTnn'n_nnAGG
Cuts at: 2396 2396
Size: 5965
EcoO109I rG'GnC_Cy
Cuts at: 2026 2027 2818 3001 2026
Size: 1 791 183 4990
Fragments arranged by size:
4990 791 183 1
EcoRI G'AATT_C
Cuts at: 3274 3652 3274
Size: 378 5587
FspI TGC'GCA
Cuts at: 2285 4471 2285
Size: 2186 3779
HaeII r_GCGC'y
Cuts at: 1678 2072 2141 2334 2415 3108 5112 1678
Size: 394 69 193 81 693 2004 2531
Fragments arranged by size:
2531 2004 693 394 193 81 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3201 3233 4606 4638 4680
Size: 32 135 32 1629 32 1373 32 42
Cuts at: 4680 4712 1373
Size: 32 2626
Fragments arranged by size:
2626 1629 1373 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2239 3309 3555 669
Size: 988 281 301 1070 246 3079
Fragments arranged by size:
3079 1070 988 301 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5965
HpaI GTT'AAC
Cuts at: 2239 3555 2239
Size: 1316 4649
KasI G'GCGC_C
Cuts at: 2137 2330 2411 2137
Size: 193 81 5691
Fragments arranged by size:
5691 193 81
KpnI G_GTAC'C
Cuts at: 3290 3290
Size: 5965
MfeI C'AATT_G
Cuts at: 3564 3564
Size: 5965
MluI A'CGCG_T
Cuts at: 5841 5841
Size: 5965
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5079 5263 1305
Size: 3774 184 2007
Fragments arranged by size:
3774 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2657 10
Size: 54 1425 343 825 3318
Fragments arranged by size:
3318 1425 825 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2600 3923 4282 4441 518
Size: 648 1434 1323 359 159 2042
Fragments arranged by size:
2042 1434 1323 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2176 2311 2353 2382 2683 3381 4058 5206
Size: 570 135 42 29 301 698 677 1148
Cuts at: 5206 5451 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 698 677 570 301 245 135
42 29
NaeI GCC'GGC
Cuts at: 2329 2345 2329
Size: 16 5949
NarI GG'CG_CC
Cuts at: 2138 2331 2412 2138
Size: 193 81 5691
Fragments arranged by size:
5691 193 81
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5413
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5965
NgoMIV G'CCGG_C
Cuts at: 2327 2343 2327
Size: 16 5949
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5965
NotI GC'GGCC_GC
Cuts at: 3412 3412
Size: 5965
NsiI A_TGCA'T
Cuts at: 3162 3162
Size: 5965
NspI r_CATG'y
Cuts at: 1132 1229 3323 4868 1132
Size: 97 2094 1545 2229
Fragments arranged by size:
2229 2094 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2889 1348
Size: 1541 4424
PciI A'CATG_T
Cuts at: 4864 4864
Size: 5965
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5965
PfoI T'CCnGG_A
Cuts at: 2874 2874
Size: 5965
PmeI GTTT'AAAC
Cuts at: 3269 3269
Size: 5965
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4034 4066 5547 5579 4034
Size: 32 1481 32 4420
Fragments arranged by size:
4420 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2818 3001 2818
Size: 183 5782
PshAI GACnn'nnGTC
Cuts at: 1613 2046 2475 1613
Size: 433 429 5103
Fragments arranged by size:
5103 433 429
PsiI TTA'TAA
Cuts at: 3535 3535
Size: 5965
PspOMI G'GGCC_C
Cuts at: 2026 2026
Size: 5965
PstI C_TGCA'G
Cuts at: 830 2634 3182 3317 830
Size: 1804 548 135 3478
Fragments arranged by size:
3478 1804 548 135
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5965
PvuII CAG'CTG
Cuts at: 2311 2311
Size: 5965
SacII CC_GC'GG
Cuts at: 2684 2684
Size: 5965
SalI G'TCGA_C
Cuts at: 1936 3307 1936
Size: 1371 4594
SbfI CC_TGCA'GG
Cuts at: 3182 3317 3182
Size: 135 5830
ScaI AGT'ACT
Cuts at: 4213 5925 4213
Size: 1712 4253
SfcI C'TryA_G
Cuts at: 826 1045 2630 3178 3313 4448 5129 5320 826
Size: 219 1585 548 135 1135 681 191 1471
Fragments arranged by size:
1585 1471 1135 681 548 219 191 135
SfoI GGC'GCC
Cuts at: 2139 2332 2413 2139
Size: 193 81 5691
Fragments arranged by size:
5691 193 81
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5965
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5965
SmaI CCC'GGG
Cuts at: 1872 2024 3292 1872
Size: 152 1268 4545
Fragments arranged by size:
4545 1268 152
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2725 2813 2930 4069 4970 5232
Size: 197 926 782 88 117 1139 901 262
Cuts at: 5232 820
Size: 1553
Fragments arranged by size:
1553 1139 926 901 782 262 197 117
88
SnaBI TAC'GTA
Cuts at: 493 3756 493
Size: 3263 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5965
SphI G_CATG'C
Cuts at: 1132 3323 1132
Size: 2191 3774
SspI AAT'ATT
Cuts at: 5 52 3889 5
Size: 47 3837 2081
Fragments arranged by size:
3837 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5965
StyI C'CwwG_G
Cuts at: 513 1065 3404 513
Size: 552 2339 3074
Fragments arranged by size:
3074 2339 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2267 3992 4009 4162 4347 4792 325
Size: 1255 687 1725 17 153 185 445 1498
Fragments arranged by size:
1725 1498 1255 687 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4211 5923 96
Size: 274 80 33 51 1443 2234 1712 138
Fragments arranged by size:
2234 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5925 5957 5925
Size: 32 5933
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5965
XbaI T'CTAG_A
Cuts at: 2782 3301 2782
Size: 519 5446
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2527 2527
Size: 5965
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5965
XmaI C'CCGG_G
Cuts at: 1870 2022 3290 1870
Size: 152 1268 4545
Fragments arranged by size:
4545 1268 152
XmnI GAAnn'nnTTC
Cuts at: 4094 4094
Size: 5965
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3773 276
Size: 53 83 186 936 2239 2468
Fragments arranged by size:
2468 2239 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BplI BsiWI BstXI
BstZ17I EcoICRI EcoRV FalI FseI FspAI NruI PacI
PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI
SfiI SrfI SwaI