Restriction map for
pFN21AE5588
(Circular) (Six-base) MAPSORT of: pFN21AE5588.seq Check: 7632 from: 1 to: 5836
pFN21AE5588 5836 bp
With 182 enzymes: SgfI *
January 30, 2014 17:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2504 2504
Size: 5836
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3646 278
Size: 53 83 186 936 2110 2468
Fragments arranged by size:
2468 2110 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3179 1937
Size: 1242 4594
Acc65I G'GTAC_C
Cuts at: 3157 3157
Size: 5836
AclI AA'CG_TT
Cuts at: 1563 3963 4336 1563
Size: 2400 373 3063
Fragments arranged by size:
3063 2400 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3899 5283 5551 1632
Size: 2267 1384 268 1917
Fragments arranged by size:
2267 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5639
AflIII A'CryG_T
Cuts at: 1203 4735 5712 1203
Size: 3532 977 1327
Fragments arranged by size:
3532 1327 977
AgeI A'CCGG_T
Cuts at: 3012 4667 3012
Size: 1655 4181
AhdI GACnn_n'nnGTC
Cuts at: 4565 4565
Size: 5836
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5836
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5836
AlwNI CAG_nnn'CTG
Cuts at: 2323 2809 3579 5151 2323
Size: 486 770 1572 3008
Fragments arranged by size:
3008 1572 770 486
ApaLI G'TGCA_C
Cuts at: 3893 5049 3893
Size: 1156 4680
ApoI r'AATT_y
Cuts at: 1493 2132 3145 3371 3523 5632 1493
Size: 639 1013 226 152 2109 1697
Fragments arranged by size:
2109 1697 1013 639 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4390 160
Size: 872 3358 1606
Fragments arranged by size:
3358 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
AvaI C'yCGr_G
Cuts at: 1870 1943 2056 2684 2759 2783 2825 3161 1870
Size: 73 113 628 75 24 42 336 4545
Fragments arranged by size:
4545 628 336 113 75 73 42 24
BamHI G'GATC_C
Cuts at: 3166 3533 3166
Size: 367 5469
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2124 2779 3157 4612 618
Size: 325 217 627 337 655 378 1455 1842
Fragments arranged by size:
1842 1455 655 627 378 337 325 217
BanII G_rGCy'C
Cuts at: 1391 2340 2580 2685 2825 2832 3155 1391
Size: 949 240 105 140 7 323 4072
Fragments arranged by size:
4072 949 323 240 140 105 7
BbeI G_GCGC'C
Cuts at: 2783 2783
Size: 5836
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4865
BbvCI CC'TCA_GC
Cuts at: 2146 2146
Size: 5836
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4027 4061 1903
Size: 34 2090 34 3678
Fragments arranged by size:
3678 2090 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3728 4938 3728
Size: 1210 4626
BclI T'GATC_A
Cuts at: 1548 2241 1548
Size: 693 5143
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4447 136
Size: 107 122 71 631 605 2775 1525
Fragments arranged by size:
2775 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5831 5831
Size: 5836
BlpI GC'TnA_GC
Cuts at: 3253 3253
Size: 5836
Bme1580I G_kGCm'C
Cuts at: 1790 2127 2406 2802 2918 3897 5053 1790
Size: 337 279 396 116 979 1156 2573
Fragments arranged by size:
2573 1156 979 396 337 279 116
BmgBI CAC'GTC
Cuts at: 1318 2260 1318
Size: 942 4894
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3567 4525 442
Size: 416 53 83 399 24 2150 958 1753
Fragments arranged by size:
2150 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5836
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2539 2539
Size: 5836
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2830 2981 4496 1239
Size: 1591 151 1515 2579
Fragments arranged by size:
2579 1591 1515 151
Bpu10I CC'TnA_GC
Cuts at: 2146 2146
Size: 5836
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2936 3961 4826 5124 2936
Size: 1025 865 298 3648
Fragments arranged by size:
3648 1025 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2642 2848 4499 882
Size: 617 1143 206 1651 2219
Fragments arranged by size:
2219 1651 1143 617 206
BsaAI yAC'GTr
Cuts at: 493 1204 2554 2671 3627 493
Size: 711 1350 117 956 2702
Fragments arranged by size:
2702 1350 956 711 117
BsaBI GATnn'nnATC
Cuts at: 5830 5830
Size: 5836
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2780 3643 4025 4660
Size: 53 83 186 936 1247 863 382 635
Cuts at: 4660 275
Size: 1451
Fragments arranged by size:
1451 1247 936 863 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3012 4269 4667 4941 5088 5499 3012
Size: 1257 398 274 147 411 3349
Fragments arranged by size:
3349 1257 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2177 2207 1178
Size: 30 165 30 774 30 4807
Fragments arranged by size:
4807 774 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2198 2570 2726 1186
Size: 1012 372 156 4296
Fragments arranged by size:
4296 1012 372 156
BseYI C'CCAG_C
Cuts at: 2309 2802 2809 5039 5535 2309
Size: 493 7 2230 496 2610
Fragments arranged by size:
2610 2230 496 493 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2201 2482 2756 3140 1163
Size: 573 465 281 274 384 3859
Fragments arranged by size:
3859 573 465 384 281 274
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3013 3286 4047 5075 1540
Size: 275 186 1012 273 761 1028 2301
Fragments arranged by size:
2301 1028 1012 761 275 273 186
BsiHKAI G_wGCw'C
Cuts at: 2580 3897 3982 5053 2580
Size: 1317 85 1071 3363
Fragments arranged by size:
3363 1317 1071 85
BsmI GAATG_Cn'
Cuts at: 3347 3440 3347
Size: 93 5743
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2127 2340 2406 2580 2685 2802 2825
Size: 399 337 213 66 174 105 117 23
Cuts at: 2825 2832 2918 3155 3897 3982 5053 1391
Size: 7 86 237 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 337 237 213 174
117 105 86 85 66 23 7
BspEI T'CCGG_A
Cuts at: 5499 5499
Size: 5836
BspHI T'CATG_A
Cuts at: 3725 5455 3725
Size: 1730 4106
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2504 3191 844
Size: 1660 687 3489
Fragments arranged by size:
3489 1660 687
BsrBI CCG'CTC
Cuts at: 1394 1823 3723 1394
Size: 429 1900 3507
Fragments arranged by size:
3507 1900 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4331 4505 65
Size: 1183 3083 174 1396
Fragments arranged by size:
3083 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2943 3012 4480 4667 1700
Size: 110 1133 69 1468 187 2869
Fragments arranged by size:
2869 1468 1133 187 110 69
BsrGI T'GTAC_A
Cuts at: 96 2193 96
Size: 2097 3739
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5836
BssSI C'ACGA_G
Cuts at: 2639 3896 4908 2639
Size: 1257 1012 3567
Fragments arranged by size:
3567 1257 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5836
BstBI TT'CG_AA
Cuts at: 4640 4640
Size: 5836
BstEII G'GTnAC_C
Cuts at: 1184 2491 3079 1184
Size: 1307 588 3941
Fragments arranged by size:
3941 1307 588
BstYI r'GATC_y
Cuts at: 1972 2883 3166 3533 3918 3935 5387 5473 5485
Size: 911 283 367 385 17 1452 86 12
Cuts at: 5485 5831 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 911 385 367 346 283 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2720 2720
Size: 5836
BtgI C'CryG_G
Cuts at: 513 1065 2929 3114 513
Size: 552 1864 185 3235
Fragments arranged by size:
3235 1864 552 185
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5097
Fragments arranged by size:
5097 683 56
BtsI GCAGTG_nn'
Cuts at: 3352 4146 4166 3352
Size: 794 20 5022
Fragments arranged by size:
5022 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5836
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3013 3048 529
Size: 35 2449 35 3317
Fragments arranged by size:
3317 2449 35 35
DraI TTT'AAA
Cuts at: 3140 3140
Size: 5836
DraIII CAC_nnn'GTG
Cuts at: 2250 2862 2250
Size: 612 5224
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4843 809
Size: 507 3527 1802
Fragments arranged by size:
3527 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2598 2605 3283
Size: 54 1425 50 275 18 768 7 678
Cuts at: 3283 4172 5801 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 768 678 275 54 50
43 18 7
EagI C'GGCC_G
Cuts at: 1537 1812 3283 1537
Size: 275 1471 4090
Fragments arranged by size:
4090 1471 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3766 1353
Size: 2413 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4423 4797 4943 387
Size: 1064 138 2834 374 146 1280
Fragments arranged by size:
2834 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2578 2578
Size: 5836
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2830 2981 3899 4496 5283 5551 1239
Size: 393 1198 151 918 597 787 268 1524
Fragments arranged by size:
1524 1198 918 787 597 393 268 151
EcoO109I rG'GnC_Cy
Cuts at: 2188 2206 2716 2764 2788 3108 2188
Size: 18 510 48 24 320 4916
Fragments arranged by size:
4916 510 320 48 24 18
EcoRI G'AATT_C
Cuts at: 3145 3523 3145
Size: 378 5458
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2188 2299 2905 2188
Size: 111 606 5119
Fragments arranged by size:
5119 606 111
FspI TGC'GCA
Cuts at: 4342 4342
Size: 5836
HaeII r_GCGC'y
Cuts at: 1678 2783 4983 1678
Size: 1105 2200 2531
Fragments arranged by size:
2531 2200 1105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2039 2071 2986 3018 3027
Size: 32 135 32 467 32 915 32 9
Cuts at: 3027 3059 4477 4509 4551 4583 1373
Size: 32 1418 32 42 32 2626
Fragments arranged by size:
2626 1418 915 467 135 42 32 32
32 32 32 32 32 9
HincII GTy'rAC
Cuts at: 669 1657 1938 3180 3426 669
Size: 988 281 1242 246 3079
Fragments arranged by size:
3079 1242 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5836
HpaI GTT'AAC
Cuts at: 3426 3426
Size: 5836
KasI G'GCGC_C
Cuts at: 2779 2779
Size: 5836
KpnI G_GTAC'C
Cuts at: 3161 3161
Size: 5836
MfeI C'AATT_G
Cuts at: 3435 3435
Size: 5836
MluI A'CGCG_T
Cuts at: 5712 5712
Size: 5836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2400 4950 5134 1305
Size: 1095 2550 184 2007
Fragments arranged by size:
2550 2007 1095 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2600 10
Size: 54 1425 343 768 3246
Fragments arranged by size:
3246 1425 768 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3794 4153 4312 518
Size: 648 2628 359 159 2042
Fragments arranged by size:
2628 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2215 2481 2590 2604 2626 2770 2809 2968
Size: 609 266 109 14 22 144 39 159
Cuts at: 2968 3252 3929 5077 5322 1606
Size: 284 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 609 284 266 245 159
144 109 39 22 14
NarI GG'CG_CC
Cuts at: 2780 2780
Size: 5836
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5284
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5836
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5836
NotI GC'GGCC_GC
Cuts at: 3283 3283
Size: 5836
NspI r_CATG'y
Cuts at: 1132 1229 3194 4739 1132
Size: 97 1965 1545 2229
Fragments arranged by size:
2229 1965 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2352 1348
Size: 1004 4832
PciI A'CATG_T
Cuts at: 4735 4735
Size: 5836
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2809 2935 1108
Size: 1701 126 4009
Fragments arranged by size:
4009 1701 126
PfoI T'CCnGG_A
Cuts at: 2699 2699
Size: 5836
PmeI GTTT'AAAC
Cuts at: 3140 3140
Size: 5836
PmlI CAC'GTG
Cuts at: 2554 2554
Size: 5836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2777 2809 3905 3937 5418 5450 2777
Size: 32 1096 32 1481 32 3163
Fragments arranged by size:
3163 1481 1096 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2716 2764 3108 2716
Size: 48 344 5444
Fragments arranged by size:
5444 344 48
PshAI GACnn'nnGTC
Cuts at: 1613 2634 1613
Size: 1021 4815
PsiI TTA'TAA
Cuts at: 3406 3406
Size: 5836
PstI C_TGCA'G
Cuts at: 830 2214 3188 830
Size: 1384 974 3478
Fragments arranged by size:
3478 1384 974
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5836
PvuII CAG'CTG
Cuts at: 2215 2590 2626 2809 2968 2215
Size: 375 36 183 159 5083
Fragments arranged by size:
5083 375 183 159 36
SacI G_AGCT'C
Cuts at: 2580 2580
Size: 5836
SalI G'TCGA_C
Cuts at: 1936 3178 1936
Size: 1242 4594
SanDI GG'GwC_CC
Cuts at: 2764 2764
Size: 5836
SbfI CC_TGCA'GG
Cuts at: 3188 3188
Size: 5836
ScaI AGT'ACT
Cuts at: 2171 4084 5796 2171
Size: 1913 1712 2211
Fragments arranged by size:
2211 1913 1712
SfcI C'TryA_G
Cuts at: 826 1045 2210 3184 4319 5000 5191 826
Size: 219 1165 974 1135 681 191 1471
Fragments arranged by size:
1471 1165 1135 974 681 219 191
SfoI GGC'GCC
Cuts at: 2781 2781
Size: 5836
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5836
SmaI CCC'GGG
Cuts at: 1872 2827 3163 1872
Size: 955 336 4545
Fragments arranged by size:
4545 955 336
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2951 3940 4841 5103 820
Size: 197 926 1008 989 901 262 1553
Fragments arranged by size:
1553 1008 989 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3627 493
Size: 3134 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5836
SphI G_CATG'C
Cuts at: 1132 3194 1132
Size: 2062 3774
SspI AAT'ATT
Cuts at: 5 52 3760 5
Size: 47 3708 2081
Fragments arranged by size:
3708 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5836
StyI C'CwwG_G
Cuts at: 513 1065 2676 2976 3275 513
Size: 552 1611 300 299 3074
Fragments arranged by size:
3074 1611 552 300 299
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3863 3880 4033 4218 4663 325
Size: 1255 2283 17 153 185 445 1498
Fragments arranged by size:
2283 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2169 2193 2727
Size: 274 80 33 51 1443 192 24 534
Cuts at: 2727 4082 5794 96
Size: 1355 1712 138
Fragments arranged by size:
1712 1443 1355 534 274 192 138 80
51 33 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5796 5828 5796
Size: 32 5804
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5836
XbaI T'CTAG_A
Cuts at: 3172 3172
Size: 5836
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2551 2551
Size: 5836
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5836
XmaI C'CCGG_G
Cuts at: 1870 2825 3161 1870
Size: 955 336 4545
Fragments arranged by size:
4545 955 336
XmnI GAAnn'nnTTC
Cuts at: 3965 3965
Size: 5836
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3644 276
Size: 53 83 186 936 2110 2468
Fragments arranged by size:
2468 2110 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstXI BstZ17I EcoNI EcoRV FseI FspAI NaeI
NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII
SacII SapI SexAI SfiI SrfI SwaI