Restriction map for
pFN21AE5586
(Circular) (Six-base) MAPSORT of: pFN21AE5586.seq Check: 6278 from: 1 to: 5818
pFN21AE5586 5818 bp
With 182 enzymes: SgfI *
January 30, 2014 17:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3628 278
Size: 53 83 186 936 2092 2468
Fragments arranged by size:
2468 2092 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2789 3161 1937
Size: 852 372 4594
Fragments arranged by size:
4594 852 372
Acc65I G'GTAC_C
Cuts at: 3139 3139
Size: 5818
AclI AA'CG_TT
Cuts at: 1563 3945 4318 1563
Size: 2382 373 3063
Fragments arranged by size:
3063 2382 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2425 2957 3881 5265 5533 1632
Size: 793 532 924 1384 268 1917
Fragments arranged by size:
1917 1384 924 793 532 268
AfeI AGC'GCT
Cuts at: 2295 2295
Size: 5818
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5621
AflIII A'CryG_T
Cuts at: 1203 4717 5694 1203
Size: 3514 977 1327
Fragments arranged by size:
3514 1327 977
AgeI A'CCGG_T
Cuts at: 4649 4649
Size: 5818
AhdI GACnn_n'nnGTC
Cuts at: 4547 4547
Size: 5818
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5818
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2984 1184
Size: 1800 4018
AlwNI CAG_nnn'CTG
Cuts at: 2104 2189 2746 2845 3561 5133 2104
Size: 85 557 99 716 1572 2789
Fragments arranged by size:
2789 1572 716 557 99 85
ApaLI G'TGCA_C
Cuts at: 3875 5031 3875
Size: 1156 4662
ApoI r'AATT_y
Cuts at: 1493 3127 3353 3505 5614 1493
Size: 1634 226 152 2109 1697
Fragments arranged by size:
2109 1697 1634 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4372 160
Size: 872 3340 1606
Fragments arranged by size:
3340 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5818
AvaI C'yCGr_G
Cuts at: 1870 1943 2280 2468 2594 3143 1870
Size: 73 337 188 126 549 4545
Fragments arranged by size:
4545 549 337 188 126 73
BamHI G'GATC_C
Cuts at: 2212 2590 2937 3148 3515 2212
Size: 378 347 211 367 4515
Fragments arranged by size:
4515 378 367 347 211
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2345 3139 4594 618
Size: 325 217 627 558 794 1455 1842
Fragments arranged by size:
1842 1455 794 627 558 325 217
BanII G_rGCy'C
Cuts at: 1391 2582 3073 3137 1391
Size: 1191 491 64 4072
Fragments arranged by size:
4072 1191 491 64
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2971 928
Size: 971 1072 3775
Fragments arranged by size:
3775 1072 971
BbvCI CC'TCA_GC
Cuts at: 2387 2387
Size: 5818
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4009 4043 1903
Size: 34 2072 34 3678
Fragments arranged by size:
3678 2072 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3710 4920 3710
Size: 1210 4608
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5818
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2056 2091 2657
Size: 107 122 71 631 605 384 35 566
Cuts at: 2657 4429 136
Size: 1772 1525
Fragments arranged by size:
1772 1525 631 605 566 384 122 107
71 35
BglII A'GATC_T
Cuts at: 5813 5813
Size: 5818
BlpI GC'TnA_GC
Cuts at: 2576 3235 2576
Size: 659 5159
Bme1580I G_kGCm'C
Cuts at: 1790 2052 2232 2910 3879 5035 1790
Size: 262 180 678 969 1156 2573
Fragments arranged by size:
2573 1156 969 678 262 180
BmgBI CAC'GTC
Cuts at: 1318 2257 1318
Size: 939 4879
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3549 4507 442
Size: 416 53 83 399 24 2132 958 1753
Fragments arranged by size:
2132 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2141 1056
Size: 1085 4733
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2211 2423 2853 3038 4478 1239
Size: 972 212 430 185 1440 2579
Fragments arranged by size:
2579 1440 972 430 212 185
Bpu10I CC'TnA_GC
Cuts at: 2387 2387
Size: 5818
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3943 4808 5106 3943
Size: 865 298 4655
Fragments arranged by size:
4655 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4481 882
Size: 617 2982 2219
Fragments arranged by size:
2982 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2047 2779 3609 493
Size: 711 843 732 830 2702
Fragments arranged by size:
2702 843 830 732 711
BsaBI GATnn'nnATC
Cuts at: 5812 5812
Size: 5818
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2265 3625 4007 4642
Size: 53 83 186 936 732 1360 382 635
Cuts at: 4642 275
Size: 1451
Fragments arranged by size:
1451 1360 936 732 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4251 4649 4923 5070 5481 4251
Size: 398 274 147 411 4588
Fragments arranged by size:
4588 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5593
Fragments arranged by size:
5593 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5818
BseYI C'CCAG_C
Cuts at: 2231 2296 2599 2661 5021 5517 2231
Size: 65 303 62 2360 496 2532
Fragments arranged by size:
2532 2360 496 303 65 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2491 1163
Size: 573 755 4490
Fragments arranged by size:
4490 755 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3268 4029 5057 1540
Size: 275 186 1267 761 1028 2301
Fragments arranged by size:
2301 1267 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3073 3879 3964 5035 3073
Size: 806 85 1071 3856
Fragments arranged by size:
3856 1071 806 85
BsmI GAATG_Cn'
Cuts at: 3329 3422 3329
Size: 93 5725
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2232 2582 2910 3073 3137 3879
Size: 399 262 180 350 328 163 64 742
Cuts at: 3879 3964 5035 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 350 328 262 180
163 85 64
BspEI T'CCGG_A
Cuts at: 5481 5481
Size: 5818
BspHI T'CATG_A
Cuts at: 3707 5437 3707
Size: 1730 4088
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2085 3173 844
Size: 1241 1088 3489
Fragments arranged by size:
3489 1241 1088
BsrBI CCG'CTC
Cuts at: 1394 1823 3705 1394
Size: 429 1882 3507
Fragments arranged by size:
3507 1882 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4313 4487 65
Size: 1183 3065 174 1396
Fragments arranged by size:
3065 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2267 2378 4462 4649 1700
Size: 110 457 111 2084 187 2869
Fragments arranged by size:
2869 2084 457 187 111 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5818
BssHII G'CGCG_C
Cuts at: 1925 2407 1925
Size: 482 5336
BssSI C'ACGA_G
Cuts at: 2909 3878 4890 2909
Size: 969 1012 3837
Fragments arranged by size:
3837 1012 969
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5818
BstBI TT'CG_AA
Cuts at: 4622 4622
Size: 5818
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5818
BstYI r'GATC_y
Cuts at: 1972 2212 2590 2892 2937 3148 3515 3900 3917
Size: 240 378 302 45 211 367 385 17
Cuts at: 3917 5369 5455 5467 5813 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 378 367 346 302 240
211 86 45 17 12
BstZ17I GTA'TAC
Cuts at: 2790 2790
Size: 5818
BtgI C'CryG_G
Cuts at: 513 1065 2004 2361 2419 513
Size: 552 939 357 58 3912
Fragments arranged by size:
3912 939 552 357 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2020 487
Size: 683 56 794 4285
Fragments arranged by size:
4285 794 683 56
BtsI GCAGTG_nn'
Cuts at: 3334 4128 4148 3334
Size: 794 20 5004
Fragments arranged by size:
5004 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5818
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3031 3066 529
Size: 35 2467 35 3281
Fragments arranged by size:
3281 2467 35 35
DraI TTT'AAA
Cuts at: 3122 3122
Size: 5818
DraIII CAC_nnn'GTG
Cuts at: 2227 2227
Size: 5818
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4825 809
Size: 507 3509 1802
Fragments arranged by size:
3509 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3265 4154
Size: 54 1425 50 275 18 177 1258 889
Cuts at: 4154 5783 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1258 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3265 1537
Size: 275 1453 4090
Fragments arranged by size:
4090 1453 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2093 3748 1353
Size: 740 1655 3423
Fragments arranged by size:
3423 1655 740
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2276 4405 4779 4925 387
Size: 1064 138 687 2129 374 146 1280
Fragments arranged by size:
2129 1280 1064 687 374 146 138
EcoICRI GAG'CTC
Cuts at: 3071 3071
Size: 5818
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2211 2423 2425 2853 2957 3038 3881
Size: 393 579 212 2 428 104 81 843
Cuts at: 3881 4478 5265 5533 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 843 787 597 579 428 393 268
212 104 81 2
EcoRI G'AATT_C
Cuts at: 3127 3505 3127
Size: 378 5440
FspI TGC'GCA
Cuts at: 2147 4324 2147
Size: 2177 3641
HaeII r_GCGC'y
Cuts at: 1678 2086 2238 2274 2297 4965 1678
Size: 408 152 36 23 2668 2531
Fragments arranged by size:
2668 2531 408 152 36 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2922 2954 4459 4491 4533
Size: 32 135 32 1350 32 1505 32 42
Cuts at: 4533 4565 1373
Size: 32 2626
Fragments arranged by size:
2626 1505 1350 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3162 3408 669
Size: 988 281 1224 246 3079
Fragments arranged by size:
3079 1224 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5818
HpaI GTT'AAC
Cuts at: 3408 3408
Size: 5818
KpnI G_GTAC'C
Cuts at: 3143 3143
Size: 5818
MfeI C'AATT_G
Cuts at: 3417 3417
Size: 5818
MluI A'CGCG_T
Cuts at: 5694 5694
Size: 5818
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4932 5116 1305
Size: 3627 184 2007
Fragments arranged by size:
3627 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3996
Fragments arranged by size:
3996 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3776 4135 4294 518
Size: 648 2610 359 159 2042
Fragments arranged by size:
2610 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2151 2200 2421 2575 3234 3911 5059 5304
Size: 545 49 221 154 659 677 1148 245
Cuts at: 5304 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 659 545 245 221 154
49
NaeI GCC'GGC
Cuts at: 2269 2380 2269
Size: 111 5707
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4327
Fragments arranged by size:
4327 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5818
NgoMIV G'CCGG_C
Cuts at: 2267 2378 2267
Size: 111 5707
NheI G'CTAG_C
Cuts at: 1052 2137 1052
Size: 1085 4733
NotI GC'GGCC_GC
Cuts at: 3265 3265
Size: 5818
NspI r_CATG'y
Cuts at: 1132 1229 3176 4721 1132
Size: 97 1947 1545 2229
Fragments arranged by size:
2229 1947 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2855 1348
Size: 1507 4311
PciI A'CATG_T
Cuts at: 4717 4717
Size: 5818
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2104 2845 1108
Size: 996 741 4081
Fragments arranged by size:
4081 996 741
PfoI T'CCnGG_A
Cuts at: 3015 3015
Size: 5818
PmeI GTTT'AAAC
Cuts at: 3122 3122
Size: 5818
PmlI CAC'GTG
Cuts at: 2047 2779 2047
Size: 732 5086
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3887 3919 5400 5432 3887
Size: 32 1481 32 4273
Fragments arranged by size:
4273 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5818
PsiI TTA'TAA
Cuts at: 3388 3388
Size: 5818
PstI C_TGCA'G
Cuts at: 830 2082 2306 2658 2775 2905 3170 830
Size: 1252 224 352 117 130 265 3478
Fragments arranged by size:
3478 1252 352 265 224 130 117
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5818
SacI G_AGCT'C
Cuts at: 3073 3073
Size: 5818
SacII CC_GC'GG
Cuts at: 2422 2422
Size: 5818
SalI G'TCGA_C
Cuts at: 1936 3160 1936
Size: 1224 4594
SbfI CC_TGCA'GG
Cuts at: 2658 3170 2658
Size: 512 5306
ScaI AGT'ACT
Cuts at: 4066 5778 4066
Size: 1712 4106
SexAI A'CCwGG_T
Cuts at: 2730 2730
Size: 5818
SfcI C'TryA_G
Cuts at: 826 1045 2078 2302 2654 2771 2901 3166 4301
Size: 219 1033 224 352 117 130 265 1135
Cuts at: 4301 4982 5173 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1033 681 352 265 224 219
191 130 117
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5818
SgrAI Cr'CCGG_yG
Cuts at: 1700 2267 1700
Size: 567 5251
SmaI CCC'GGG
Cuts at: 1872 2282 2470 3145 1872
Size: 410 188 675 4545
Fragments arranged by size:
4545 675 410 188
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3922 4823 5085 820
Size: 197 926 1979 901 262 1553
Fragments arranged by size:
1979 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3609 493
Size: 3116 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5818
SphI G_CATG'C
Cuts at: 1132 3176 1132
Size: 2044 3774
SspI AAT'ATT
Cuts at: 5 52 3742 5
Size: 47 3690 2081
Fragments arranged by size:
3690 2081 47
StuI AGG'CCT
Cuts at: 1517 2769 1517
Size: 1252 4566
StyI C'CwwG_G
Cuts at: 513 1065 2004 2499 3257 513
Size: 552 939 495 758 3074
Fragments arranged by size:
3074 939 758 552 495
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3845 3862 4015 4200 4645 325
Size: 1255 2265 17 153 185 445 1498
Fragments arranged by size:
2265 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4064 5776 96
Size: 274 80 33 51 1443 2087 1712 138
Fragments arranged by size:
2087 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5778 5810 5778
Size: 32 5786
Tth111I GACn'n_nGTC
Cuts at: 1316 2159 1316
Size: 843 4975
XbaI T'CTAG_A
Cuts at: 3154 3154
Size: 5818
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2359 2689 2818 2359
Size: 330 129 5359
Fragments arranged by size:
5359 330 129
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5818
XmaI C'CCGG_G
Cuts at: 1870 2280 2468 3143 1870
Size: 410 188 675 4545
Fragments arranged by size:
4545 675 410 188
XmnI GAAnn'nnTTC
Cuts at: 3947 3947
Size: 5818
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3626 276
Size: 53 83 186 936 2092 2468
Fragments arranged by size:
2468 2092 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PstI PvuI SacI SacII SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI Bsu36I EcoNI EcoO109I EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PpuMI PspOMI PspXI PsrI PvuII RsrII SanDI SapI
SfiI SfoI SrfI SwaI