Restriction map for
pFN21AE5585
(Circular) (Six-base) MAPSORT of: pFN21AE5585.seq Check: 1093 from: 1 to: 5815
pFN21AE5585 5815 bp
With 182 enzymes: SgfI *
January 30, 2014 17:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3625 278
Size: 53 83 186 936 2089 2468
Fragments arranged by size:
2468 2089 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2262 2412 3158 1937
Size: 325 150 746 4594
Fragments arranged by size:
4594 746 325 150
Acc65I G'GTAC_C
Cuts at: 3136 3136
Size: 5815
AclI AA'CG_TT
Cuts at: 1563 3942 4315 1563
Size: 2379 373 3063
Fragments arranged by size:
3063 2379 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2111 2874 3878 5262 5530 1632
Size: 479 763 1004 1384 268 1917
Fragments arranged by size:
1917 1384 1004 763 479 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5618
AflIII A'CryG_T
Cuts at: 1203 4714 5691 1203
Size: 3511 977 1327
Fragments arranged by size:
3511 1327 977
AgeI A'CCGG_T
Cuts at: 4646 4646
Size: 5815
AhdI GACnn_n'nnGTC
Cuts at: 4544 4544
Size: 5815
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5815
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2484 2567 1184
Size: 1300 83 4432
Fragments arranged by size:
4432 1300 83
AlwNI CAG_nnn'CTG
Cuts at: 3558 5130 3558
Size: 1572 4243
ApaLI G'TGCA_C
Cuts at: 3872 5028 3872
Size: 1156 4659
ApoI r'AATT_y
Cuts at: 1493 2097 2640 3124 3350 3502 5611 1493
Size: 604 543 484 226 152 2109 1697
Fragments arranged by size:
2109 1697 604 543 484 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4369 160
Size: 872 3337 1606
Fragments arranged by size:
3337 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5815
AvaI C'yCGr_G
Cuts at: 1870 1943 3140 1870
Size: 73 1197 4545
Fragments arranged by size:
4545 1197 73
BamHI G'GATC_C
Cuts at: 3145 3512 3145
Size: 367 5448
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3136 4591 618
Size: 325 217 627 1349 1455 1842
Fragments arranged by size:
1842 1455 1349 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3134 1391
Size: 1743 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4844
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4006 4040 1903
Size: 34 2069 34 3678
Fragments arranged by size:
3678 2069 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3707 4917 3707
Size: 1210 4605
BclI T'GATC_A
Cuts at: 1548 2760 1548
Size: 1212 4603
BfrBI ATG'CAT
Cuts at: 2925 2925
Size: 5815
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4426 136
Size: 107 122 71 631 605 2754 1525
Fragments arranged by size:
2754 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5810 5810
Size: 5815
BlpI GC'TnA_GC
Cuts at: 3232 3232
Size: 5815
Bme1580I G_kGCm'C
Cuts at: 1790 3876 5032 1790
Size: 2086 1156 2573
Fragments arranged by size:
2573 2086 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5815
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3546 4504 442
Size: 416 53 83 399 24 2129 958 1753
Fragments arranged by size:
2129 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5815
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2738 4475 1239
Size: 1499 1737 2579
Fragments arranged by size:
2579 1737 1499
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3940 4805 5103 3940
Size: 865 298 4652
Fragments arranged by size:
4652 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4478 882
Size: 617 2979 2219
Fragments arranged by size:
2979 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3606 493
Size: 711 2402 2702
Fragments arranged by size:
2702 2402 711
BsaBI GATnn'nnATC
Cuts at: 5809 5809
Size: 5815
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3622 4004 4639 275
Size: 53 83 186 936 2089 382 635 1451
Fragments arranged by size:
2089 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4248 4646 4920 5067 5478 4248
Size: 398 274 147 411 4585
Fragments arranged by size:
4585 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2569 2599 2929 2959 1178
Size: 30 165 30 1166 30 330 30 4034
Fragments arranged by size:
4034 1166 330 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3073 1186
Size: 1887 3928
BseYI C'CCAG_C
Cuts at: 2486 5018 5514 2486
Size: 2532 496 2787
Fragments arranged by size:
2787 2532 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2713 1163
Size: 573 977 4265
Fragments arranged by size:
4265 977 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3265 4026 5054 1540
Size: 275 186 1264 761 1028 2301
Fragments arranged by size:
2301 1264 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3876 3961 5032 3876
Size: 85 1071 4659
Fragments arranged by size:
4659 1071 85
BsmI GAATG_Cn'
Cuts at: 2589 2626 3326 3419 2589
Size: 37 700 93 4985
Fragments arranged by size:
4985 700 93 37
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3134 3876 3961 5032 1391
Size: 399 1344 742 85 1071 2174
Fragments arranged by size:
2174 1344 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5478 5478
Size: 5815
BspHI T'CATG_A
Cuts at: 2568 3704 5434 2568
Size: 1136 1730 2949
Fragments arranged by size:
2949 1730 1136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3170 844
Size: 2326 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3702 1394
Size: 429 1879 3507
Fragments arranged by size:
3507 1879 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2265 2892 4310 4484 65
Size: 1183 1017 627 1418 174 1396
Fragments arranged by size:
1418 1396 1183 1017 627 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4459 4646 1700
Size: 110 2649 187 2869
Fragments arranged by size:
2869 2649 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5815
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5815
BssSI C'ACGA_G
Cuts at: 3875 4887 3875
Size: 1012 4803
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5815
BstBI TT'CG_AA
Cuts at: 4619 4619
Size: 5815
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5815
BstYI r'GATC_y
Cuts at: 1972 2885 3145 3512 3897 3914 5366 5452 5464
Size: 913 260 367 385 17 1452 86 12
Cuts at: 5464 5810 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 913 385 367 346 260 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2514 513
Size: 552 1449 3814
Fragments arranged by size:
3814 1449 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5076
Fragments arranged by size:
5076 683 56
BtsI GCAGTG_nn'
Cuts at: 3331 4125 4145 3331
Size: 794 20 5001
Fragments arranged by size:
5001 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5815
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5780
DraI TTT'AAA
Cuts at: 3119 3119
Size: 5815
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4822 809
Size: 507 3506 1802
Fragments arranged by size:
3506 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2535 3262 4151
Size: 54 1425 50 275 18 705 727 889
Cuts at: 4151 5780 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 727 705 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3262 1537
Size: 275 1450 4090
Fragments arranged by size:
4090 1450 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2328 2566 3745 1353
Size: 975 238 1179 3423
Fragments arranged by size:
3423 1179 975 238
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4402 4776 4922 387
Size: 1064 138 2813 374 146 1280
Fragments arranged by size:
2813 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2111 2738 2874 3878 4475 5262 5530
Size: 393 479 627 136 1004 597 787 268
Cuts at: 5530 1239
Size: 1524
Fragments arranged by size:
1524 1004 787 627 597 479 393 268
136
EcoO109I rG'GnC_Cy
Cuts at: 2617 2617
Size: 5815
EcoRI G'AATT_C
Cuts at: 3124 3502 3124
Size: 378 5437
FspI TGC'GCA
Cuts at: 4321 4321
Size: 5815
HaeII r_GCGC'y
Cuts at: 1678 2863 4962 1678
Size: 1185 2099 2531
Fragments arranged by size:
2531 2099 1185
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2133 2165 4456 4488 4530
Size: 32 135 32 561 32 2291 32 42
Cuts at: 4530 4562 1373
Size: 32 2626
Fragments arranged by size:
2626 2291 561 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3159 3405 669
Size: 988 281 1221 246 3079
Fragments arranged by size:
3079 1221 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2179 748
Size: 1431 4384
HpaI GTT'AAC
Cuts at: 3405 3405
Size: 5815
KpnI G_GTAC'C
Cuts at: 3140 3140
Size: 5815
MfeI C'AATT_G
Cuts at: 3414 3414
Size: 5815
MluI A'CGCG_T
Cuts at: 5691 5691
Size: 5815
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2119 4929 5113 1305
Size: 814 2810 184 2007
Fragments arranged by size:
2810 2007 814 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2537 10
Size: 54 1425 343 705 3288
Fragments arranged by size:
3288 1425 705 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2047 2513 2594 3773 4132 4291 518
Size: 648 881 466 81 1179 359 159 2042
Fragments arranged by size:
2042 1179 881 648 466 359 159 81
MspA1I CmG'CkG
Cuts at: 1606 3231 3908 5056 5301 1606
Size: 1625 677 1148 245 2120
Fragments arranged by size:
2120 1625 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2514 513
Size: 552 1449 3814
Fragments arranged by size:
3814 1449 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5815
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5815
NotI GC'GGCC_GC
Cuts at: 3262 3262
Size: 5815
NsiI A_TGCA'T
Cuts at: 2927 2927
Size: 5815
NspI r_CATG'y
Cuts at: 1132 1229 2170 2290 3173 4718 1132
Size: 97 941 120 883 1545 2229
Fragments arranged by size:
2229 1545 941 883 120 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5815
PciI A'CATG_T
Cuts at: 4714 4714
Size: 5815
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5815
PfoI T'CCnGG_A
Cuts at: 2367 2367
Size: 5815
PmeI GTTT'AAAC
Cuts at: 3119 3119
Size: 5815
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3884 3916 5397 5429 3884
Size: 32 1481 32 4270
Fragments arranged by size:
4270 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5815
PsiI TTA'TAA
Cuts at: 3385 3385
Size: 5815
PstI C_TGCA'G
Cuts at: 830 3167 830
Size: 2337 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5815
SalI G'TCGA_C
Cuts at: 1936 3157 1936
Size: 1221 4594
SapI GCTCTTCn'nnn_
Cuts at: 2328 2328
Size: 5815
SbfI CC_TGCA'GG
Cuts at: 3167 3167
Size: 5815
ScaI AGT'ACT
Cuts at: 4063 5775 4063
Size: 1712 4103
SfcI C'TryA_G
Cuts at: 826 1045 3163 4298 4979 5170 826
Size: 219 2118 1135 681 191 1471
Fragments arranged by size:
2118 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5815
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5815
SmaI CCC'GGG
Cuts at: 1872 3142 1872
Size: 1270 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3919 4820 5082 820
Size: 197 926 1976 901 262 1553
Fragments arranged by size:
1976 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3606 493
Size: 3113 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5815
SphI G_CATG'C
Cuts at: 1132 3173 1132
Size: 2041 3774
SspI AAT'ATT
Cuts at: 5 52 2982 3106 3739 5
Size: 47 2930 124 633 2081
Fragments arranged by size:
2930 2081 633 124 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5815
StyI C'CwwG_G
Cuts at: 513 1065 2157 2514 3254 513
Size: 552 1092 357 740 3074
Fragments arranged by size:
3074 1092 740 552 357
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3842 3859 4012 4197 4642 325
Size: 1255 2262 17 153 185 445 1498
Fragments arranged by size:
2262 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2340 4061 5773
Size: 274 80 33 51 1443 363 1721 1712
Cuts at: 5773 96
Size: 138
Fragments arranged by size:
1721 1712 1443 363 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2569 2601 5775 5807 2569
Size: 32 3174 32 2577
Fragments arranged by size:
3174 2577 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5815
XbaI T'CTAG_A
Cuts at: 3151 3151
Size: 5815
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5815
XmaI C'CCGG_G
Cuts at: 1870 3140 1870
Size: 1270 4545
XmnI GAAnn'nnTTC
Cuts at: 3944 3944
Size: 5815
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3623 276
Size: 53 83 186 936 2089 2468
Fragments arranged by size:
2468 2089 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI