Restriction map for
pFN21AE5576
(Circular) (Six-base) MAPSORT of: pFN21AE5576.seq Check: 4862 from: 1 to: 5875
pFN21AE5576 5875 bp
With 182 enzymes: SgfI *
January 30, 2014 17:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3685 278
Size: 53 83 186 936 2149 2468
Fragments arranged by size:
2468 2149 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2778 2922 2994 3066 3218 1937
Size: 841 144 72 72 152 4594
Fragments arranged by size:
4594 841 152 144 72 72
Acc65I G'GTAC_C
Cuts at: 3196 3196
Size: 5875
AclI AA'CG_TT
Cuts at: 1563 4002 4375 1563
Size: 2439 373 3063
Fragments arranged by size:
3063 2439 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2348 3938 5322 5590 1632
Size: 716 1590 1384 268 1917
Fragments arranged by size:
1917 1590 1384 716 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5678
AflIII A'CryG_T
Cuts at: 1203 4774 5751 1203
Size: 3571 977 1327
Fragments arranged by size:
3571 1327 977
AgeI A'CCGG_T
Cuts at: 4706 4706
Size: 5875
AhdI GACnn_n'nnGTC
Cuts at: 4604 4604
Size: 5875
AleI CACnn'nnGTG
Cuts at: 1166 3088 1166
Size: 1922 3953
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2132 1184
Size: 948 4927
AlwNI CAG_nnn'CTG
Cuts at: 3618 5190 3618
Size: 1572 4303
ApaI G_GGCC'C
Cuts at: 2339 2339
Size: 5875
ApaLI G'TGCA_C
Cuts at: 3932 5088 3932
Size: 1156 4719
ApoI r'AATT_y
Cuts at: 1493 2737 2881 2953 3025 3184 3410 3562 5671
Size: 1244 144 72 72 159 226 152 2109
Cuts at: 5671 1493
Size: 1697
Fragments arranged by size:
2109 1697 1244 226 159 152 144 72
72
AseI AT'TA_AT
Cuts at: 160 1032 4429 160
Size: 872 3397 1606
Fragments arranged by size:
3397 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5875
AvaI C'yCGr_G
Cuts at: 1870 1943 3200 1870
Size: 73 1257 4545
Fragments arranged by size:
4545 1257 73
BamHI G'GATC_C
Cuts at: 3205 3572 3205
Size: 367 5508
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3196 4651 618
Size: 325 217 627 1409 1455 1842
Fragments arranged by size:
1842 1455 1409 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2339 3194 1391
Size: 948 855 4072
Fragments arranged by size:
4072 948 855
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2058 928
Size: 971 159 4745
Fragments arranged by size:
4745 971 159
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4066 4100 1903
Size: 34 2129 34 3678
Fragments arranged by size:
3678 2129 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3767 4977 3767
Size: 1210 4665
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5875
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4486 136
Size: 107 122 71 631 605 2814 1525
Fragments arranged by size:
2814 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5870 5870
Size: 5875
BlpI GC'TnA_GC
Cuts at: 3292 3292
Size: 5875
Bme1580I G_kGCm'C
Cuts at: 1790 2339 3095 3936 5092 1790
Size: 549 756 841 1156 2573
Fragments arranged by size:
2573 1156 841 756 549
BmgBI CAC'GTC
Cuts at: 1318 2275 2384 1318
Size: 957 109 4809
Fragments arranged by size:
4809 957 109
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2056 3606 4564
Size: 416 53 83 399 24 639 1550 958
Cuts at: 4564 442
Size: 1753
Fragments arranged by size:
1753 1550 958 639 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2085 1056
Size: 1029 4846
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2029 4535 1239
Size: 790 2506 2579
Fragments arranged by size:
2579 2506 790
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4000 4865 5163 4000
Size: 865 298 4712
Fragments arranged by size:
4712 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4538 882
Size: 617 3039 2219
Fragments arranged by size:
3039 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3666 493
Size: 711 2462 2702
Fragments arranged by size:
2702 2462 711
BsaBI GATnn'nnATC
Cuts at: 5869 5869
Size: 5875
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3682 4064 4699 275
Size: 53 83 186 936 2149 382 635 1451
Fragments arranged by size:
2149 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4308 4706 4980 5127 5538 4308
Size: 398 274 147 411 4645
Fragments arranged by size:
4645 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5650
Fragments arranged by size:
5650 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2394 2397 1186
Size: 1208 3 4664
Fragments arranged by size:
4664 1208 3
BseYI C'CCAG_C
Cuts at: 2091 2332 5078 5574 2091
Size: 241 2746 496 2392
Fragments arranged by size:
2746 2392 496 241
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2396 1163
Size: 573 660 4642
Fragments arranged by size:
4642 660 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3325 4086 5114 1540
Size: 275 186 1324 761 1028 2301
Fragments arranged by size:
2301 1324 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2368 3936 4021 5092 2368
Size: 1568 85 1071 3151
Fragments arranged by size:
3151 1568 1071 85
BsmI GAATG_Cn'
Cuts at: 3386 3479 3386
Size: 93 5782
BsmBI CGTCTCn'nnnn_
Cuts at: 3165 3165
Size: 5875
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2339 2368 3095 3194 3936 4021 5092
Size: 399 549 29 727 99 742 85 1071
Cuts at: 5092 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 727 549 399 99 85
29
BspEI T'CCGG_A
Cuts at: 5538 5538
Size: 5875
BspHI T'CATG_A
Cuts at: 3764 5494 3764
Size: 1730 4145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2466 3230 844
Size: 1622 764 3489
Fragments arranged by size:
3489 1622 764
BsrBI CCG'CTC
Cuts at: 1394 1823 3762 1394
Size: 429 1939 3507
Fragments arranged by size:
3507 1939 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4370 4544 65
Size: 1183 3122 174 1396
Fragments arranged by size:
3122 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4519 4706 1700
Size: 110 2709 187 2869
Fragments arranged by size:
2869 2709 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5875
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5875
BssSI C'ACGA_G
Cuts at: 3935 4947 3935
Size: 1012 4863
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2726 2870 2942 3014 794
Size: 1932 144 72 72 3655
Fragments arranged by size:
3655 1932 144 72 72
BstBI TT'CG_AA
Cuts at: 4679 4679
Size: 5875
BstEII G'GTnAC_C
Cuts at: 1184 2608 2968 3040 1184
Size: 1424 360 72 4019
Fragments arranged by size:
4019 1424 360 72
BstYI r'GATC_y
Cuts at: 1972 3205 3572 3957 3974 5426 5512 5524 5870
Size: 1233 367 385 17 1452 86 12 346
Cuts at: 5870 1972
Size: 1977
Fragments arranged by size:
1977 1452 1233 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2779 2923 2995 3067 2779
Size: 144 72 72 5587
Fragments arranged by size:
5587 144 72 72
BtgI C'CryG_G
Cuts at: 513 1065 2314 513
Size: 552 1249 4074
Fragments arranged by size:
4074 1249 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5136
Fragments arranged by size:
5136 683 56
BtsI GCAGTG_nn'
Cuts at: 2682 2826 3095 3391 4185 4205 2682
Size: 144 269 296 794 20 4352
Fragments arranged by size:
4352 794 296 269 144 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5875
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2370 2405 529
Size: 35 1806 35 3999
Fragments arranged by size:
3999 1806 35 35
DraI TTT'AAA
Cuts at: 3179 3179
Size: 5875
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2279 4882 809
Size: 507 963 2603 1802
Fragments arranged by size:
2603 1802 963 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3322 4211 5840
Size: 54 1425 50 275 18 1492 889 1629
Cuts at: 5840 8
Size: 43
Fragments arranged by size:
1629 1492 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3322 1537
Size: 275 1510 4090
Fragments arranged by size:
4090 1510 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3163 3805 1353
Size: 1810 642 3423
Fragments arranged by size:
3423 1810 642
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2449 4462 4836 4982 387
Size: 1064 138 860 2013 374 146 1280
Fragments arranged by size:
2013 1280 1064 860 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2029 2348 3938 4535 5322 5590 1239
Size: 393 397 319 1590 597 787 268 1524
Fragments arranged by size:
1590 1524 787 597 397 393 319 268
EcoO109I rG'GnC_Cy
Cuts at: 2336 2336
Size: 5875
EcoRI G'AATT_C
Cuts at: 3184 3562 3184
Size: 378 5497
EcoRV GAT'ATC
Cuts at: 2621 2693 2765 2837 2909 2981 3053 2621
Size: 72 72 72 72 72 72 5443
Fragments arranged by size:
5443 72 72 72 72 72 72
FspI TGC'GCA
Cuts at: 4381 4381
Size: 5875
HaeII r_GCGC'y
Cuts at: 1678 5022 1678
Size: 3344 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4516 4548 4590 4622 1373
Size: 32 135 32 2944 32 42 32 2626
Fragments arranged by size:
2944 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3219 3465 669
Size: 988 281 1281 246 3079
Fragments arranged by size:
3079 1281 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5875
HpaI GTT'AAC
Cuts at: 3465 3465
Size: 5875
KpnI G_GTAC'C
Cuts at: 3200 3200
Size: 5875
MfeI C'AATT_G
Cuts at: 3474 3474
Size: 5875
MluI A'CGCG_T
Cuts at: 5751 5751
Size: 5875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4989 5173 1305
Size: 3684 184 2007
Fragments arranged by size:
3684 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4053
Fragments arranged by size:
4053 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2313 3088 3136 3833 4192 4351 518
Size: 648 1147 775 48 697 359 159 2042
Fragments arranged by size:
2042 1147 775 697 648 359 159 48
MspA1I CmG'CkG
Cuts at: 1606 2075 3291 3968 5116 5361 1606
Size: 469 1216 677 1148 245 2120
Fragments arranged by size:
2120 1216 1148 677 469 245
NcoI C'CATG_G
Cuts at: 513 1065 2314 513
Size: 552 1249 4074
Fragments arranged by size:
4074 1249 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5875
NheI G'CTAG_C
Cuts at: 1052 2081 1052
Size: 1029 4846
NotI GC'GGCC_GC
Cuts at: 3322 3322
Size: 5875
NspI r_CATG'y
Cuts at: 1132 1229 3233 4778 1132
Size: 97 2004 1545 2229
Fragments arranged by size:
2229 2004 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5875
PciI A'CATG_T
Cuts at: 4774 4774
Size: 5875
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5875
PmeI GTTT'AAAC
Cuts at: 3179 3179
Size: 5875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3944 3976 5457 5489 3944
Size: 32 1481 32 4330
Fragments arranged by size:
4330 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5875
PsiI TTA'TAA
Cuts at: 3445 3445
Size: 5875
PspOMI G'GGCC_C
Cuts at: 2335 2335
Size: 5875
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2179 2211 2179
Size: 32 5843
PstI C_TGCA'G
Cuts at: 830 2080 2463 3227 830
Size: 1250 383 764 3478
Fragments arranged by size:
3478 1250 764 383
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5875
PvuII CAG'CTG
Cuts at: 2075 2075
Size: 5875
SalI G'TCGA_C
Cuts at: 1936 3217 1936
Size: 1281 4594
SbfI CC_TGCA'GG
Cuts at: 3227 3227
Size: 5875
ScaI AGT'ACT
Cuts at: 4123 5835 4123
Size: 1712 4163
SexAI A'CCwGG_T
Cuts at: 2527 2527
Size: 5875
SfcI C'TryA_G
Cuts at: 826 1045 2076 2459 3223 4358 5039 5230 826
Size: 219 1031 383 764 1135 681 191 1471
Fragments arranged by size:
1471 1135 1031 764 681 383 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5875
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5875
SmaI CCC'GGG
Cuts at: 1872 3202 1872
Size: 1330 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3979 4880 5142 820
Size: 197 926 2036 901 262 1553
Fragments arranged by size:
2036 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3666 493
Size: 3173 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5875
SphI G_CATG'C
Cuts at: 1132 3233 1132
Size: 2101 3774
SspI AAT'ATT
Cuts at: 5 52 3799 5
Size: 47 3747 2081
Fragments arranged by size:
3747 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5875
StyI C'CwwG_G
Cuts at: 513 1065 2086 2314 3314 513
Size: 552 1021 228 1000 3074
Fragments arranged by size:
3074 1021 1000 552 228
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2075 2083 3140 3902 3919 4072 4257
Size: 1255 495 8 1057 762 17 153 185
Cuts at: 4257 4702 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1057 762 495 445 185 153
17 8
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4121 5833 96
Size: 274 80 33 51 1443 2144 1712 138
Fragments arranged by size:
2144 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2464 2496 5835 5867 2464
Size: 32 3339 32 2472
Fragments arranged by size:
3339 2472 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5875
XbaI T'CTAG_A
Cuts at: 3211 3211
Size: 5875
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5875
XmaI C'CCGG_G
Cuts at: 1870 3200 1870
Size: 1330 4545
XmnI GAAnn'nnTTC
Cuts at: 4004 4004
Size: 5875
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3683 276
Size: 53 83 186 936 2149 2468
Fragments arranged by size:
2468 2149 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI Bsu36I DraIII EcoICRI
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspXI RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI
XcmI