Restriction map for
pFN21AE5575
(Circular) (Six-base) MAPSORT of: pFN21AE5575.seq Check: 7895 from: 1 to: 6091
pFN21AE5575 6091 bp
With 182 enzymes: SgfI *
January 30, 2014 17:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2280 2280
Size: 6091
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3901 278
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3434 1937
Size: 1497 4594
Acc65I G'GTAC_C
Cuts at: 2793 3412 2793
Size: 619 5472
AclI AA'CG_TT
Cuts at: 1563 4218 4591 1563
Size: 2655 373 3063
Fragments arranged by size:
3063 2655 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2141 4154 5538 5806 1632
Size: 509 2013 1384 268 1917
Fragments arranged by size:
2013 1917 1384 509 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5894
AflIII A'CryG_T
Cuts at: 1203 4990 5967 1203
Size: 3787 977 1327
Fragments arranged by size:
3787 1327 977
AgeI A'CCGG_T
Cuts at: 4922 4922
Size: 6091
AhdI GACnn_n'nnGTC
Cuts at: 4820 4820
Size: 6091
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6091
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3178 1184
Size: 1994 4097
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2687 2719 2687
Size: 32 6059
AlwNI CAG_nnn'CTG
Cuts at: 2065 2245 2692 2779 3032 3050 3834 5406 2065
Size: 180 447 87 253 18 784 1572 2750
Fragments arranged by size:
2750 1572 784 447 253 180 87 18
ApaI G_GGCC'C
Cuts at: 2075 2507 2075
Size: 432 5659
ApaLI G'TGCA_C
Cuts at: 2513 4148 5304 2513
Size: 1635 1156 3300
Fragments arranged by size:
3300 1635 1156
ApoI r'AATT_y
Cuts at: 1493 3400 3626 3778 5887 1493
Size: 1907 226 152 2109 1697
Fragments arranged by size:
2109 1907 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3305 4645 160
Size: 872 2273 1340 1606
Fragments arranged by size:
2273 1606 1340 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
AvaI C'yCGr_G
Cuts at: 1870 1943 2171 2756 3416 1870
Size: 73 228 585 660 4545
Fragments arranged by size:
4545 660 585 228 73
BamHI G'GATC_C
Cuts at: 3421 3788 3421
Size: 367 5724
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2793 2931 3185 3412 4867
Size: 325 217 627 1006 138 254 227 1455
Cuts at: 4867 618
Size: 1842
Fragments arranged by size:
1842 1455 1006 627 325 254 227 217
138
BanII G_rGCy'C
Cuts at: 1391 2075 2507 2766 3410 1391
Size: 684 432 259 644 4072
Fragments arranged by size:
4072 684 644 432 259
BbeI G_GCGC'C
Cuts at: 2935 2935
Size: 6091
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2390 928
Size: 971 491 4629
Fragments arranged by size:
4629 971 491
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4282 4316 1903
Size: 34 2345 34 3678
Fragments arranged by size:
3678 2345 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3983 5193 3983
Size: 1210 4881
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6091
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4702 136
Size: 107 122 71 631 605 3030 1525
Fragments arranged by size:
3030 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6086 6086
Size: 6091
BlpI GC'TnA_GC
Cuts at: 3508 3508
Size: 6091
Bme1580I G_kGCm'C
Cuts at: 1790 2075 2507 2517 4152 5308 1790
Size: 285 432 10 1635 1156 2573
Fragments arranged by size:
2573 1635 1156 432 285 10
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6091
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3371 3822 4780
Size: 416 53 83 399 24 1954 451 958
Cuts at: 4780 442
Size: 1753
Fragments arranged by size:
1954 1753 958 451 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6091
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2968 2968
Size: 6091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2132 2379 2725 3003 4751 1239
Size: 893 247 346 278 1748 2579
Fragments arranged by size:
2579 1748 893 346 278 247
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4216 5081 5379 4216
Size: 865 298 4928
Fragments arranged by size:
4928 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4754 882
Size: 617 3255 2219
Fragments arranged by size:
3255 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3882 493
Size: 711 2678 2702
Fragments arranged by size:
2702 2678 711
BsaBI GATnn'nnATC
Cuts at: 6085 6085
Size: 6091
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2932 3898 4280 4915
Size: 53 83 186 936 1399 966 382 635
Cuts at: 4915 275
Size: 1451
Fragments arranged by size:
1451 1399 966 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2277 4524 4922 5196 5343 5754 2277
Size: 2247 398 274 147 411 2614
Fragments arranged by size:
2614 2247 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2699 2729 3050 3080 1178
Size: 30 165 30 1296 30 321 30 4189
Fragments arranged by size:
4189 1296 321 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2026 2344 2774 1186
Size: 838 2 318 430 4503
Fragments arranged by size:
4503 838 430 318 2
BseYI C'CCAG_C
Cuts at: 2645 2772 5294 5790 2645
Size: 127 2522 496 2946
Fragments arranged by size:
2946 2522 496 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2446 1163
Size: 573 710 4808
Fragments arranged by size:
4808 710 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2656 3541 4302 5330 1540
Size: 275 186 655 885 761 1028 2301
Fragments arranged by size:
2301 1028 885 761 655 275 186
BsiHKAI G_wGCw'C
Cuts at: 2181 2517 2766 3267 4152 4237 5308 2181
Size: 336 249 501 885 85 1071 2964
Fragments arranged by size:
2964 1071 885 501 336 249 85
BsmI GAATG_Cn'
Cuts at: 3602 3695 3602
Size: 93 5998
BsmBI CGTCTCn'nnnn_
Cuts at: 2320 2320
Size: 6091
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2075 2181 2507 2517 2766 3267 3410
Size: 399 285 106 326 10 249 501 143
Cuts at: 3410 4152 4237 5308 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 501 399 326 285 249
143 106 85 10
BspEI T'CCGG_A
Cuts at: 5754 5754
Size: 6091
BspHI T'CATG_A
Cuts at: 3270 3980 5710 3270
Size: 710 1730 3651
Fragments arranged by size:
3651 1730 710
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2280 3222 3446 844
Size: 1436 942 224 3489
Fragments arranged by size:
3489 1436 942 224
BsrBI CCG'CTC
Cuts at: 1394 1823 2606 3978 1394
Size: 429 783 1372 3507
Fragments arranged by size:
3507 1372 783 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3150 4586 4760 65
Size: 1183 1902 1436 174 1396
Fragments arranged by size:
1902 1436 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2651 3095 4735 4922 1700
Size: 110 841 444 1640 187 2869
Fragments arranged by size:
2869 1640 841 444 187 110
BsrGI T'GTAC_A
Cuts at: 96 2247 96
Size: 2151 3940
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6091
BssSI C'ACGA_G
Cuts at: 4151 5163 4151
Size: 1012 5079
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3032 3115 794
Size: 2238 83 3770
Fragments arranged by size:
3770 2238 83
BstBI TT'CG_AA
Cuts at: 4895 4895
Size: 6091
BstEII G'GTnAC_C
Cuts at: 1184 3091 1184
Size: 1907 4184
BstXI CCAn_nnnn'nTGG
Cuts at: 2209 2449 2209
Size: 240 5851
BstYI r'GATC_y
Cuts at: 1972 3421 3788 4173 4190 5642 5728 5740 6086
Size: 1449 367 385 17 1452 86 12 346
Cuts at: 6086 1972
Size: 1977
Fragments arranged by size:
1977 1452 1449 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2038 2967 2038
Size: 929 5162
BtgI C'CryG_G
Cuts at: 513 1065 2004 2202 2801 3069 3218 513
Size: 552 939 198 599 268 149 3386
Fragments arranged by size:
3386 939 599 552 268 198 149
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5352
Fragments arranged by size:
5352 683 56
BtsI GCAGTG_nn'
Cuts at: 3113 3607 4401 4421 3113
Size: 494 794 20 4783
Fragments arranged by size:
4783 794 494 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6091
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6056
DraI TTT'AAA
Cuts at: 3395 3395
Size: 6091
DraIII CAC_nnn'GTG
Cuts at: 3253 3253
Size: 6091
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5098 809
Size: 507 3782 1802
Fragments arranged by size:
3782 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2610 2653 2865
Size: 54 1425 50 275 18 780 43 212
Cuts at: 2865 3097 3294 3538 4427 6056 8
Size: 232 197 244 889 1629 43
Fragments arranged by size:
1629 1425 889 780 275 244 232 212
197 54 50 43 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2653 3538 1537
Size: 275 841 885 4090
Fragments arranged by size:
4090 885 841 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2138 2329 2492 4021 1353
Size: 785 191 163 1529 3423
Fragments arranged by size:
3423 1529 785 191 163
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2078 2126 4678 5052 5198 387
Size: 1064 138 489 48 2552 374 146 1280
Fragments arranged by size:
2552 1280 1064 489 374 146 138 48
EcoICRI GAG'CTC
Cuts at: 2764 2764
Size: 6091
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2132 2141 2379 2725 3003 4154 4751
Size: 393 500 9 238 346 278 1151 597
Cuts at: 4751 5538 5806 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1151 787 597 500 393 346 278
268 238 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2144 2144
Size: 6091
EcoO109I rG'GnC_Cy
Cuts at: 2071 2072 2504 2820 2071
Size: 1 432 316 5342
Fragments arranged by size:
5342 432 316 1
EcoRI G'AATT_C
Cuts at: 3400 3778 3400
Size: 378 5713
EcoRV GAT'ATC
Cuts at: 2425 2425
Size: 6091
FspI TGC'GCA
Cuts at: 2555 4597 2555
Size: 2042 4049
FspAI rTGC'GCAy
Cuts at: 2555 2555
Size: 6091
HaeII r_GCGC'y
Cuts at: 1678 2652 2726 2935 3077 5238 1678
Size: 974 74 209 142 2161 2531
Fragments arranged by size:
2531 2161 974 209 142 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2699 2731 2910 2942 2980
Size: 32 135 32 1127 32 179 32 38
Cuts at: 2980 3012 4732 4764 4806 4838 1373
Size: 32 1720 32 42 32 2626
Fragments arranged by size:
2626 1720 1127 179 135 42 38 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3435 3681 669
Size: 988 281 1497 246 3079
Fragments arranged by size:
3079 1497 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6091
HpaI GTT'AAC
Cuts at: 3681 3681
Size: 6091
KasI G'GCGC_C
Cuts at: 2931 2931
Size: 6091
KpnI G_GTAC'C
Cuts at: 2797 3416 2797
Size: 619 5472
MfeI C'AATT_G
Cuts at: 3690 3690
Size: 6091
MluI A'CGCG_T
Cuts at: 5967 5967
Size: 6091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5205 5389 1305
Size: 3900 184 2007
Fragments arranged by size:
3900 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2612 2867 3296 10
Size: 54 1425 343 780 255 429 2805
Fragments arranged by size:
2805 1425 780 429 343 255 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2879 3305 4049 4408 4567 518
Size: 648 1713 426 744 359 159 2042
Fragments arranged by size:
2042 1713 744 648 426 359 159
MspA1I CmG'CkG
Cuts at: 1606 2196 2803 3507 4184 5332 5577 1606
Size: 590 607 704 677 1148 245 2120
Fragments arranged by size:
2120 1148 704 677 607 590 245
NaeI GCC'GGC
Cuts at: 2653 2653
Size: 6091
NarI GG'CG_CC
Cuts at: 2932 2932
Size: 6091
NcoI C'CATG_G
Cuts at: 513 1065 2004 2202 513
Size: 552 939 198 4402
Fragments arranged by size:
4402 939 552 198
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6091
NgoMIV G'CCGG_C
Cuts at: 2651 2651
Size: 6091
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6091
NotI GC'GGCC_GC
Cuts at: 3538 3538
Size: 6091
NspI r_CATG'y
Cuts at: 1132 1229 3113 3449 4994 1132
Size: 97 1884 336 1545 2229
Fragments arranged by size:
2229 1884 1545 336 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6091
PciI A'CATG_T
Cuts at: 4990 4990
Size: 6091
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6091
PfoI T'CCnGG_A
Cuts at: 2288 2373 2288
Size: 85 6006
PmeI GTTT'AAAC
Cuts at: 3395 3395
Size: 6091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4160 4192 5673 5705 4160
Size: 32 1481 32 4546
Fragments arranged by size:
4546 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2820 2820
Size: 6091
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6091
PsiI TTA'TAA
Cuts at: 3661 3661
Size: 6091
PspOMI G'GGCC_C
Cuts at: 2071 2503 2071
Size: 432 5659
PstI C_TGCA'G
Cuts at: 830 2061 3037 3443 830
Size: 1231 976 406 3478
Fragments arranged by size:
3478 1231 976 406
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6091
PvuII CAG'CTG
Cuts at: 2196 2196
Size: 6091
SacI G_AGCT'C
Cuts at: 2766 2766
Size: 6091
SacII CC_GC'GG
Cuts at: 2804 2804
Size: 6091
SalI G'TCGA_C
Cuts at: 1936 3433 1936
Size: 1497 4594
SanDI GG'GwC_CC
Cuts at: 2820 2820
Size: 6091
SapI GCTCTTCn'nnn_
Cuts at: 2138 2138
Size: 6091
SbfI CC_TGCA'GG
Cuts at: 3443 3443
Size: 6091
ScaI AGT'ACT
Cuts at: 4339 6051 4339
Size: 1712 4379
SfcI C'TryA_G
Cuts at: 826 1045 2057 3033 3439 4574 5255 5446 826
Size: 219 1012 976 406 1135 681 191 1471
Fragments arranged by size:
1471 1135 1012 976 681 406 219 191
SfoI GGC'GCC
Cuts at: 2933 2933
Size: 6091
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6091
SmaI CCC'GGG
Cuts at: 1872 3418 1872
Size: 1546 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4195 5096 5358 820
Size: 197 926 2252 901 262 1553
Fragments arranged by size:
2252 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3882 493
Size: 3389 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6091
SphI G_CATG'C
Cuts at: 1132 3113 3449 1132
Size: 1981 336 3774
Fragments arranged by size:
3774 1981 336
SspI AAT'ATT
Cuts at: 5 52 4015 5
Size: 47 3963 2081
Fragments arranged by size:
3963 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6091
StyI C'CwwG_G
Cuts at: 513 1065 2004 2202 2616 3337 3530 513
Size: 552 939 198 414 721 193 3074
Fragments arranged by size:
3074 939 721 552 414 198 193
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2659 4118 4135 4288 4473 4918 325
Size: 1255 1079 1459 17 153 185 445 1498
Fragments arranged by size:
1498 1459 1255 1079 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2247 2598 3325
Size: 274 80 33 51 1443 270 351 727
Cuts at: 3325 4337 6049 96
Size: 1012 1712 138
Fragments arranged by size:
1712 1443 1012 727 351 274 270 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3050 3082 6051 6083 3050
Size: 32 2969 32 3058
Fragments arranged by size:
3058 2969 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6091
XbaI T'CTAG_A
Cuts at: 3427 3427
Size: 6091
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3076 3076
Size: 6091
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6091
XmaI C'CCGG_G
Cuts at: 1870 3416 1870
Size: 1546 4545
XmnI GAAnn'nnTTC
Cuts at: 2132 4220 2132
Size: 2088 4003
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3899 276
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BstZ17I FalI FseI NruI NsiI PacI PmlI PspXI
PsrI RsrII SexAI SfiI SrfI SwaI