Restriction map for
pFN21AE5574
(Circular) (Six-base) MAPSORT of: pFN21AE5574.seq Check: 9866 from: 1 to: 6067
pFN21AE5574 6067 bp
With 182 enzymes: SgfI *
January 30, 2014 17:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3199 3877 278
Size: 53 83 186 936 1663 678 2468
Fragments arranged by size:
2468 1663 936 678 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3267 3410 1937
Size: 1330 143 4594
Fragments arranged by size:
4594 1330 143
Acc65I G'GTAC_C
Cuts at: 3388 3388
Size: 6067
AclI AA'CG_TT
Cuts at: 1563 4194 4567 1563
Size: 2631 373 3063
Fragments arranged by size:
3063 2631 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2622 2674 4130 5514 5782 1632
Size: 990 52 1456 1384 268 1917
Fragments arranged by size:
1917 1456 1384 990 268 52
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5870
AflIII A'CryG_T
Cuts at: 1203 4966 5943 1203
Size: 3763 977 1327
Fragments arranged by size:
3763 1327 977
AgeI A'CCGG_T
Cuts at: 4898 4898
Size: 6067
AhdI GACnn_n'nnGTC
Cuts at: 4796 4796
Size: 6067
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6067
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6067
AlwNI CAG_nnn'CTG
Cuts at: 3810 5382 3810
Size: 1572 4495
ApaI G_GGCC'C
Cuts at: 2217 2217
Size: 6067
ApaLI G'TGCA_C
Cuts at: 3242 4124 5280 3242
Size: 882 1156 4029
Fragments arranged by size:
4029 1156 882
ApoI r'AATT_y
Cuts at: 1493 2048 2169 2802 3376 3602 3754 5863 1493
Size: 555 121 633 574 226 152 2109 1697
Fragments arranged by size:
2109 1697 633 574 555 226 152 121
AseI AT'TA_AT
Cuts at: 160 1032 4621 160
Size: 872 3589 1606
Fragments arranged by size:
3589 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
AvaI C'yCGr_G
Cuts at: 1870 1943 2337 3392 1870
Size: 73 394 1055 4545
Fragments arranged by size:
4545 1055 394 73
BamHI G'GATC_C
Cuts at: 3173 3397 3764 3173
Size: 224 367 5476
Fragments arranged by size:
5476 367 224
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2917 3388 4843 618
Size: 325 217 627 1130 471 1455 1842
Fragments arranged by size:
1842 1455 1130 627 471 325 217
BanII G_rGCy'C
Cuts at: 1391 2217 2814 2886 2972 3386 1391
Size: 826 597 72 86 414 4072
Fragments arranged by size:
4072 826 597 414 86 72
BbeI G_GCGC'C
Cuts at: 2921 2921
Size: 6067
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5096
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2184 2218 2226 2260 2986 3020 4258
Size: 34 247 34 8 34 726 34 1238
Cuts at: 4258 4292 1903
Size: 34 3678
Fragments arranged by size:
3678 1238 726 247 34 34 34 34
34 8
BciVI GTATCCnnnnn_n'
Cuts at: 3959 5169 3959
Size: 1210 4857
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6067
BfrBI ATG'CAT
Cuts at: 3138 3138
Size: 6067
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2249 4678 136
Size: 107 122 71 631 605 577 2429 1525
Fragments arranged by size:
2429 1525 631 605 577 122 107 71
BglII A'GATC_T
Cuts at: 6062 6062
Size: 6067
BlpI GC'TnA_GC
Cuts at: 2604 3484 2604
Size: 880 5187
Bme1580I G_kGCm'C
Cuts at: 1790 2217 2410 3246 4128 5284 1790
Size: 427 193 836 882 1156 2573
Fragments arranged by size:
2573 1156 882 836 427 193
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6067
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3798 4756 442
Size: 416 53 83 399 24 2381 958 1753
Fragments arranged by size:
2381 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6067
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2619 2619
Size: 6067
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2047 2615 2828 2956 4727 1239
Size: 808 568 213 128 1771 2579
Fragments arranged by size:
2579 1771 808 568 213 128
Bpu10I CC'TnA_GC
Cuts at: 2594 2676 2966 3354 2594
Size: 82 290 388 5307
Fragments arranged by size:
5307 388 290 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4192 5057 5355 4192
Size: 865 298 4904
Fragments arranged by size:
4904 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2572 4730 882
Size: 617 1073 2158 2219
Fragments arranged by size:
2219 2158 1073 617
BsaAI yAC'GTr
Cuts at: 493 1204 3858 493
Size: 711 2654 2702
Fragments arranged by size:
2702 2654 711
BsaBI GATnn'nnATC
Cuts at: 6061 6061
Size: 6067
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2918 3196 3874 4256
Size: 53 83 186 936 1385 278 678 382
Cuts at: 4256 4891 275
Size: 635 1451
Fragments arranged by size:
1451 1385 936 678 635 382 278 186
83 53
BsaWI w'CCGG_w
Cuts at: 3201 4500 4898 5172 5319 5730 3201
Size: 1299 398 274 147 411 3538
Fragments arranged by size:
3538 1299 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2905 2935 2984 3014 1178
Size: 30 165 30 1502 30 49 30 4231
Fragments arranged by size:
4231 1502 165 49 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2876 1186
Size: 1690 4377
BseYI C'CCAG_C
Cuts at: 2210 2216 2270 5270 5766 2210
Size: 6 54 3000 496 2511
Fragments arranged by size:
3000 2511 496 54 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2207 2633 2953 3136 1163
Size: 573 471 426 320 183 4094
Fragments arranged by size:
4094 573 471 426 320 183
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3517 4278 5306 1540
Size: 275 186 1516 761 1028 2301
Fragments arranged by size:
2301 1516 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2600 2814 2972 3246 4128 4213 5284 2600
Size: 214 158 274 882 85 1071 3383
Fragments arranged by size:
3383 1071 882 274 214 158 85
BsmI GAATG_Cn'
Cuts at: 3578 3671 3578
Size: 93 5974
BsmBI CGTCTCn'nnnn_
Cuts at: 3203 3203
Size: 6067
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2217 2410 2600 2814 2886 2972 3246
Size: 399 427 193 190 214 72 86 274
Cuts at: 3246 3386 4128 4213 5284 1391
Size: 140 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 427 399 274 214 193
190 140 86 85 72
BspEI T'CCGG_A
Cuts at: 5730 5730
Size: 6067
BspHI T'CATG_A
Cuts at: 2310 3956 5686 2310
Size: 1646 1730 2691
Fragments arranged by size:
2691 1730 1646
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3422 844
Size: 2578 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2961 3954 1394
Size: 429 1138 993 3507
Fragments arranged by size:
3507 1138 993 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4562 4736 65
Size: 1183 3314 174 1396
Fragments arranged by size:
3314 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3089 4711 4898 1700
Size: 110 1279 1622 187 2869
Fragments arranged by size:
2869 1622 1279 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6067
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6067
BssSI C'ACGA_G
Cuts at: 2609 4127 5139 2609
Size: 1518 1012 3537
Fragments arranged by size:
3537 1518 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6067
BstBI TT'CG_AA
Cuts at: 4871 4871
Size: 6067
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6067
BstXI CCAn_nnnn'nTGG
Cuts at: 2270 2270
Size: 6067
BstYI r'GATC_y
Cuts at: 1972 3173 3397 3764 4149 4166 5618 5704 5716
Size: 1201 224 367 385 17 1452 86 12
Cuts at: 5716 6062 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1201 385 367 346 224 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2487 3177 513
Size: 552 939 483 690 3403
Fragments arranged by size:
3403 939 690 552 483
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5328
Fragments arranged by size:
5328 683 56
BtsI GCAGTG_nn'
Cuts at: 2227 3244 3583 4377 4397 2227
Size: 1017 339 794 20 3897
Fragments arranged by size:
3897 1017 794 339 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6067
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6032
DraI TTT'AAA
Cuts at: 3371 3371
Size: 6067
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5074 809
Size: 507 3758 1802
Fragments arranged by size:
3758 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3514 4403 6032
Size: 54 1425 50 275 18 1684 889 1629
Cuts at: 6032 8
Size: 43
Fragments arranged by size:
1684 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3514 1537
Size: 275 1702 4090
Fragments arranged by size:
4090 1702 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3997 1353
Size: 2644 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4654 5028 5174 387
Size: 1064 138 3065 374 146 1280
Fragments arranged by size:
3065 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2812 2970 2812
Size: 158 5909
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2047 2615 2622 2674 2828 2956 4130
Size: 393 415 568 7 52 154 128 1174
Cuts at: 4130 4727 5514 5782 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1174 787 597 568 415 393 268
154 128 52 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2296 2296
Size: 6067
EcoO109I rG'GnC_Cy
Cuts at: 2067 2626 2067
Size: 559 5508
EcoRI G'AATT_C
Cuts at: 3376 3754 3376
Size: 378 5689
FspI TGC'GCA
Cuts at: 4573 4573
Size: 6067
HaeII r_GCGC'y
Cuts at: 1678 2486 2921 5214 1678
Size: 808 435 2293 2531
Fragments arranged by size:
2531 2293 808 435
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2090 2122 2905 2937 2984
Size: 32 135 32 518 32 783 32 47
Cuts at: 2984 3016 3265 3297 4708 4740 4782 4814 1373
Size: 32 249 32 1411 32 42 32 2626
Fragments arranged by size:
2626 1411 783 518 249 135 47 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3411 3657 669
Size: 988 281 1473 246 3079
Fragments arranged by size:
3079 1473 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6067
HpaI GTT'AAC
Cuts at: 3657 3657
Size: 6067
KasI G'GCGC_C
Cuts at: 2917 2917
Size: 6067
KpnI G_GTAC'C
Cuts at: 3392 3392
Size: 6067
MfeI C'AATT_G
Cuts at: 2540 3666 2540
Size: 1126 4941
MluI A'CGCG_T
Cuts at: 5943 5943
Size: 6067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2673 5181 5365 1305
Size: 1368 2508 184 2007
Fragments arranged by size:
2508 2007 1368 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4245
Fragments arranged by size:
4245 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2847 4025 4384 4543 518
Size: 648 1681 1178 359 159 2042
Fragments arranged by size:
2042 1681 1178 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2191 2270 3483 4160 5308 5553 1606
Size: 585 79 1213 677 1148 245 2120
Fragments arranged by size:
2120 1213 1148 677 585 245 79
NaeI GCC'GGC
Cuts at: 3091 3091
Size: 6067
NarI GG'CG_CC
Cuts at: 2918 2918
Size: 6067
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4576
Fragments arranged by size:
4576 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6067
NgoMIV G'CCGG_C
Cuts at: 3089 3089
Size: 6067
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6067
NotI GC'GGCC_GC
Cuts at: 3514 3514
Size: 6067
NsiI A_TGCA'T
Cuts at: 3140 3140
Size: 6067
NspI r_CATG'y
Cuts at: 1132 1229 3425 4970 1132
Size: 97 2196 1545 2229
Fragments arranged by size:
2229 2196 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2155 1348
Size: 807 5260
PciI A'CATG_T
Cuts at: 4966 4966
Size: 6067
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6067
PmeI GTTT'AAAC
Cuts at: 3371 3371
Size: 6067
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4136 4168 5649 5681 4136
Size: 32 1481 32 4522
Fragments arranged by size:
4522 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2067 2067
Size: 6067
PshAI GACnn'nnGTC
Cuts at: 1613 3195 1613
Size: 1582 4485
PsiI TTA'TAA
Cuts at: 3637 3637
Size: 6067
PspOMI G'GGCC_C
Cuts at: 2213 2213
Size: 6067
PstI C_TGCA'G
Cuts at: 830 2482 2516 3081 3419 830
Size: 1652 34 565 338 3478
Fragments arranged by size:
3478 1652 565 338 34
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6067
PvuII CAG'CTG
Cuts at: 2191 2270 2191
Size: 79 5988
SacI G_AGCT'C
Cuts at: 2814 2972 2814
Size: 158 5909
SalI G'TCGA_C
Cuts at: 1936 3409 1936
Size: 1473 4594
SbfI CC_TGCA'GG
Cuts at: 3419 3419
Size: 6067
ScaI AGT'ACT
Cuts at: 4315 6027 4315
Size: 1712 4355
SfcI C'TryA_G
Cuts at: 826 1045 2293 2478 2512 2786 3077 3415 4550
Size: 219 1248 185 34 274 291 338 1135
Cuts at: 4550 5231 5422 826
Size: 681 191 1471
Fragments arranged by size:
1471 1248 1135 681 338 291 274 219
191 185 34
SfoI GGC'GCC
Cuts at: 2919 2919
Size: 6067
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6067
SmaI CCC'GGG
Cuts at: 1872 3394 1872
Size: 1522 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2337 4171 5072 5334 820
Size: 197 926 394 1834 901 262 1553
Fragments arranged by size:
1834 1553 926 901 394 262 197
SnaBI TAC'GTA
Cuts at: 493 3858 493
Size: 3365 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6067
SphI G_CATG'C
Cuts at: 1132 3425 1132
Size: 2293 3774
SspI AAT'ATT
Cuts at: 5 52 3991 5
Size: 47 3939 2081
Fragments arranged by size:
3939 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6067
StyI C'CwwG_G
Cuts at: 513 1065 2004 2148 2988 3009 3506 513
Size: 552 939 144 840 21 497 3074
Fragments arranged by size:
3074 939 840 552 497 144 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2043 4094 4111 4264 4449 4894 325
Size: 1255 463 2051 17 153 185 445 1498
Fragments arranged by size:
2051 1498 1255 463 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4313 6025 96
Size: 274 80 33 51 1443 2336 1712 138
Fragments arranged by size:
2336 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6027 6059 6027
Size: 32 6035
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6067
XbaI T'CTAG_A
Cuts at: 3403 3403
Size: 6067
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3140 3140
Size: 6067
XhoI C'TCGA_G
Cuts at: 1943 2337 1943
Size: 394 5673
XmaI C'CCGG_G
Cuts at: 1870 3392 1870
Size: 1522 4545
XmnI GAAnn'nnTTC
Cuts at: 4196 4196
Size: 6067
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3197 3875 276
Size: 53 83 186 936 1663 678 2468
Fragments arranged by size:
2468 1663 936 678 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BsiWI
BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI
PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SrfI SwaI