Restriction map for
pFN21AE5571
(Circular) (Six-base) MAPSORT of: pFN21AE5571.seq Check: 3778 from: 1 to: 6064
pFN21AE5571 6064 bp
With 182 enzymes: SgfI *
January 30, 2014 17:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3874 278
Size: 53 83 186 936 2338 2468
Fragments arranged by size:
2468 2338 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2234 3407 1937
Size: 297 1173 4594
Fragments arranged by size:
4594 1173 297
Acc65I G'GTAC_C
Cuts at: 3385 3385
Size: 6064
AclI AA'CG_TT
Cuts at: 1563 4191 4564 1563
Size: 2628 373 3063
Fragments arranged by size:
3063 2628 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2193 2395 2978 3134 4127 5511 5779 1632
Size: 561 202 583 156 993 1384 268 1917
Fragments arranged by size:
1917 1384 993 583 561 268 202 156
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5867
AflIII A'CryG_T
Cuts at: 1203 4963 5940 1203
Size: 3760 977 1327
Fragments arranged by size:
3760 1327 977
AgeI A'CCGG_T
Cuts at: 4895 4895
Size: 6064
AhdI GACnn_n'nnGTC
Cuts at: 4793 4793
Size: 6064
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6064
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6064
AlwNI CAG_nnn'CTG
Cuts at: 3807 5379 3807
Size: 1572 4492
ApaI G_GGCC'C
Cuts at: 2211 2211
Size: 6064
ApaLI G'TGCA_C
Cuts at: 4121 5277 4121
Size: 1156 4908
ApoI r'AATT_y
Cuts at: 1493 3270 3373 3599 3751 5860 1493
Size: 1777 103 226 152 2109 1697
Fragments arranged by size:
2109 1777 1697 226 152 103
AseI AT'TA_AT
Cuts at: 160 1032 2796 4618 160
Size: 872 1764 1822 1606
Fragments arranged by size:
1822 1764 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
AvaI C'yCGr_G
Cuts at: 1870 1943 3389 1870
Size: 73 1446 4545
Fragments arranged by size:
4545 1446 73
AvrII C'CTAG_G
Cuts at: 2056 2056
Size: 6064
BamHI G'GATC_C
Cuts at: 2106 3394 3761 2106
Size: 1288 367 4409
Fragments arranged by size:
4409 1288 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3242 3385 4840 618
Size: 325 217 627 1455 143 1455 1842
Fragments arranged by size:
1842 1455 1455 627 325 217 143
BanII G_rGCy'C
Cuts at: 1391 2211 3383 1391
Size: 820 1172 4072
Fragments arranged by size:
4072 1172 820
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2938 928
Size: 971 1039 4054
Fragments arranged by size:
4054 1039 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4255 4289 1903
Size: 34 2318 34 3678
Fragments arranged by size:
3678 2318 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3956 5166 3956
Size: 1210 4854
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6064
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4675 136
Size: 107 122 71 631 605 3003 1525
Fragments arranged by size:
3003 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6059 6059
Size: 6064
BlpI GC'TnA_GC
Cuts at: 3481 3481
Size: 6064
Bme1580I G_kGCm'C
Cuts at: 1790 2211 2875 4125 5281 1790
Size: 421 664 1250 1156 2573
Fragments arranged by size:
2573 1250 1156 664 421
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6064
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3275 3322 3795
Size: 416 53 83 399 24 1858 47 473
Cuts at: 3795 4753 442
Size: 958 1753
Fragments arranged by size:
1858 1753 958 473 416 399 83 53
47 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6064
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2606 4724 1239
Size: 1367 2118 2579
Fragments arranged by size:
2579 2118 1367
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2504 4189 5054 5352 2504
Size: 1685 865 298 3216
Fragments arranged by size:
3216 1685 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4727 882
Size: 617 3228 2219
Fragments arranged by size:
3228 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2565 3855 493
Size: 711 1361 1290 2702
Fragments arranged by size:
2702 1361 1290 711
BsaBI GATnn'nnATC
Cuts at: 6058 6058
Size: 6064
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2342 3871 4253
Size: 53 83 186 936 476 333 1529 382
Cuts at: 4253 4888 275
Size: 635 1451
Fragments arranged by size:
1529 1451 936 635 476 382 333 186
83 53
BsaWI w'CCGG_w
Cuts at: 4497 4895 5169 5316 5727 4497
Size: 398 274 147 411 4834
Fragments arranged by size:
4834 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2151 2181 2216 2246 1178
Size: 30 165 30 748 30 35 30 4996
Fragments arranged by size:
4996 748 165 35 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6064
BseYI C'CCAG_C
Cuts at: 2812 5267 5763 2812
Size: 2455 496 3113
Fragments arranged by size:
3113 2455 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5491
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2150 3514 4275 5303 1540
Size: 275 186 149 1364 761 1028 2301
Fragments arranged by size:
2301 1364 1028 761 275 186 149
BsiHKAI G_wGCw'C
Cuts at: 3177 4125 4210 5281 3177
Size: 948 85 1071 3960
Fragments arranged by size:
3960 1071 948 85
BsmI GAATG_Cn'
Cuts at: 2939 3575 3668 2939
Size: 636 93 5335
Fragments arranged by size:
5335 636 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2019 2019
Size: 6064
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2211 2875 3177 3383 4125 4210 5281
Size: 399 421 664 302 206 742 85 1071
Cuts at: 5281 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 664 421 399 302 206
85
BspEI T'CCGG_A
Cuts at: 5727 5727
Size: 6064
BspHI T'CATG_A
Cuts at: 2733 3953 5683 2733
Size: 1220 1730 3114
Fragments arranged by size:
3114 1730 1220
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3419 844
Size: 2575 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3951 1394
Size: 429 2128 3507
Fragments arranged by size:
3507 2128 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2376 4559 4733 65
Size: 1183 1128 2183 174 1396
Fragments arranged by size:
2183 1396 1183 1128 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4708 4895 1700
Size: 110 2898 187 2869
Fragments arranged by size:
2898 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6064
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6064
BssSI C'ACGA_G
Cuts at: 3178 4124 5136 3178
Size: 946 1012 4106
Fragments arranged by size:
4106 1012 946
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2884 3026 794
Size: 2090 142 3832
Fragments arranged by size:
3832 2090 142
BstBI TT'CG_AA
Cuts at: 4868 4868
Size: 6064
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6064
BstXI CCAn_nnnn'nTGG
Cuts at: 2482 2911 2482
Size: 429 5635
BstYI r'GATC_y
Cuts at: 1972 2106 3394 3761 4146 4163 5615 5701 5713
Size: 134 1288 367 385 17 1452 86 12
Cuts at: 5713 6059 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1288 385 367 346 134 86
17 12
BstZ17I GTA'TAC
Cuts at: 2235 2235
Size: 6064
BtgI C'CryG_G
Cuts at: 513 1065 2004 2910 513
Size: 552 939 906 3667
Fragments arranged by size:
3667 939 906 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5325
Fragments arranged by size:
5325 683 56
BtsI GCAGTG_nn'
Cuts at: 2904 3361 3580 4374 4394 2904
Size: 457 219 794 20 4574
Fragments arranged by size:
4574 794 457 219 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6064
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6029
DraI TTT'AAA
Cuts at: 3368 3368
Size: 6064
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5071 809
Size: 507 3755 1802
Fragments arranged by size:
3755 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2147 3511 4400
Size: 54 1425 50 275 18 317 1364 889
Cuts at: 4400 6029 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1364 889 317 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2147 3511 1537
Size: 275 335 1364 4090
Fragments arranged by size:
4090 1364 335 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2414 3281 3994 1353
Size: 1061 867 713 3423
Fragments arranged by size:
3423 1061 867 713
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2243 4651 5025 5171 387
Size: 1064 138 654 2408 374 146 1280
Fragments arranged by size:
2408 1280 1064 654 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2193 2395 2606 2978 3134 4127 4724
Size: 393 561 202 211 372 156 993 597
Cuts at: 4724 5511 5779 1239
Size: 787 268 1524
Fragments arranged by size:
1524 993 787 597 561 393 372 268
211 202 156
EcoRI G'AATT_C
Cuts at: 3270 3373 3751 3270
Size: 103 378 5583
Fragments arranged by size:
5583 378 103
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2597 3144 2597
Size: 547 5517
FspI TGC'GCA
Cuts at: 4570 4570
Size: 6064
HaeII r_GCGC'y
Cuts at: 1678 3188 5211 1678
Size: 1510 2023 2531
Fragments arranged by size:
2531 2023 1510
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2151 2183 2293 2325 2734
Size: 32 135 32 579 32 110 32 409
Cuts at: 2734 2766 3115 3147 3174 3206 4705 4737 4779
Size: 32 349 32 27 32 1499 32 42
Cuts at: 4779 4811 1373
Size: 32 2626
Fragments arranged by size:
2626 1499 579 409 349 135 110 42
32 32 32 32 32 32 32 32
32 27
HincII GTy'rAC
Cuts at: 669 1657 1938 2488 2977 3408 3654 669
Size: 988 281 550 489 431 246 3079
Fragments arranged by size:
3079 988 550 489 431 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6064
HpaI GTT'AAC
Cuts at: 2488 3654 2488
Size: 1166 4898
KpnI G_GTAC'C
Cuts at: 3389 3389
Size: 6064
MfeI C'AATT_G
Cuts at: 3663 3663
Size: 6064
MluI A'CGCG_T
Cuts at: 5940 5940
Size: 6064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2084 5178 5362 1305
Size: 779 3094 184 2007
Fragments arranged by size:
3094 2007 779 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4242
Fragments arranged by size:
4242 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2192 2909 4022 4381 4540 518
Size: 648 1026 717 1113 359 159 2042
Fragments arranged by size:
2042 1113 1026 717 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2972 3480 4157 5305 5550 1606
Size: 1366 508 677 1148 245 2120
Fragments arranged by size:
2120 1366 1148 677 508 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2910 513
Size: 552 939 906 3667
Fragments arranged by size:
3667 939 906 552
NdeI CA'TA_TG
Cuts at: 387 2899 387
Size: 2512 3552
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6064
NotI GC'GGCC_GC
Cuts at: 3511 3511
Size: 6064
NspI r_CATG'y
Cuts at: 1132 1229 2510 3422 4967 1132
Size: 97 1281 912 1545 2229
Fragments arranged by size:
2229 1545 1281 912 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6064
PciI A'CATG_T
Cuts at: 4963 4963
Size: 6064
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2916 3236 1108
Size: 1808 320 3936
Fragments arranged by size:
3936 1808 320
PfoI T'CCnGG_A
Cuts at: 2109 2109
Size: 6064
PmeI GTTT'AAAC
Cuts at: 3368 3368
Size: 6064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4133 4165 5646 5678 4133
Size: 32 1481 32 4519
Fragments arranged by size:
4519 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6064
PsiI TTA'TAA
Cuts at: 2690 3634 2690
Size: 944 5120
PspOMI G'GGCC_C
Cuts at: 2207 2207
Size: 6064
PstI C_TGCA'G
Cuts at: 830 3416 830
Size: 2586 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6064
PvuII CAG'CTG
Cuts at: 2972 2972
Size: 6064
SalI G'TCGA_C
Cuts at: 1936 3406 1936
Size: 1470 4594
SbfI CC_TGCA'GG
Cuts at: 3416 3416
Size: 6064
ScaI AGT'ACT
Cuts at: 4312 6024 4312
Size: 1712 4352
SfcI C'TryA_G
Cuts at: 826 1045 3412 4547 5228 5419 826
Size: 219 2367 1135 681 191 1471
Fragments arranged by size:
2367 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6064
SmaI CCC'GGG
Cuts at: 1872 3391 1872
Size: 1519 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2519 4168 5069 5331 820
Size: 197 926 576 1649 901 262 1553
Fragments arranged by size:
1649 1553 926 901 576 262 197
SnaBI TAC'GTA
Cuts at: 493 3855 493
Size: 3362 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6064
SphI G_CATG'C
Cuts at: 1132 2510 3422 1132
Size: 1378 912 3774
Fragments arranged by size:
3774 1378 912
SspI AAT'ATT
Cuts at: 5 52 3988 5
Size: 47 3936 2081
Fragments arranged by size:
3936 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6064
StyI C'CwwG_G
Cuts at: 513 1065 2004 2056 2910 3503 513
Size: 552 939 52 854 593 3074
Fragments arranged by size:
3074 939 854 593 552 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3052 4091 4108 4261 4446 4891 325
Size: 1255 1472 1039 17 153 185 445 1498
Fragments arranged by size:
1498 1472 1255 1039 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3309 4310 6022
Size: 274 80 33 51 1443 1332 1001 1712
Cuts at: 6022 96
Size: 138
Fragments arranged by size:
1712 1443 1332 1001 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6024 6056 6024
Size: 32 6032
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6064
XbaI T'CTAG_A
Cuts at: 3400 3400
Size: 6064
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3337 3337
Size: 6064
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6064
XmaI C'CCGG_G
Cuts at: 1870 3389 1870
Size: 1519 4545
XmnI GAAnn'nnTTC
Cuts at: 4193 4193
Size: 6064
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3872 276
Size: 53 83 186 936 2338 2468
Fragments arranged by size:
2468 2338 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI