Restriction map for
pFN21AE5569
(Circular) (Six-base) MAPSORT of: pFN21AE5569.seq Check: 1380 from: 1 to: 6043
pFN21AE5569 6043 bp
With 182 enzymes: SgfI *
January 30, 2014 17:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2523 3853 278
Size: 53 83 186 936 987 1330 2468
Fragments arranged by size:
2468 1330 987 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3386 1937
Size: 1449 4594
Acc65I G'GTAC_C
Cuts at: 3157 3364 3157
Size: 207 5836
AclI AA'CG_TT
Cuts at: 1563 4170 4543 1563
Size: 2607 373 3063
Fragments arranged by size:
3063 2607 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2096 2141 2189 2354 2507 2608 2822 4106
Size: 464 45 48 165 153 101 214 1284
Cuts at: 4106 5490 5758 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1284 464 268 214 165 153
101 48 45
AfeI AGC'GCT
Cuts at: 3324 3324
Size: 6043
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5846
AflIII A'CryG_T
Cuts at: 1203 2031 4942 5919 1203
Size: 828 2911 977 1327
Fragments arranged by size:
2911 1327 977 828
AgeI A'CCGG_T
Cuts at: 4874 4874
Size: 6043
AhdI GACnn_n'nnGTC
Cuts at: 4772 4772
Size: 6043
AleI CACnn'nnGTG
Cuts at: 1166 2793 1166
Size: 1627 4416
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3110 1184
Size: 1926 4117
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3101 3133 3101
Size: 32 6011
AlwNI CAG_nnn'CTG
Cuts at: 2845 3786 5358 2845
Size: 941 1572 3530
Fragments arranged by size:
3530 1572 941
ApaLI G'TGCA_C
Cuts at: 2024 4100 5256 2024
Size: 2076 1156 2811
Fragments arranged by size:
2811 2076 1156
ApoI r'AATT_y
Cuts at: 1493 3352 3578 3730 5839 1493
Size: 1859 226 152 2109 1697
Fragments arranged by size:
2109 1859 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4597 160
Size: 872 3565 1606
Fragments arranged by size:
3565 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6043
AvaI C'yCGr_G
Cuts at: 1870 1943 3161 3193 3309 3339 3368 1870
Size: 73 1218 32 116 30 29 4545
Fragments arranged by size:
4545 1218 116 73 32 30 29
BamHI G'GATC_C
Cuts at: 3373 3740 3373
Size: 367 5676
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3157 3239 3364 4819 618
Size: 325 217 627 1370 82 125 1455 1842
Fragments arranged by size:
1842 1455 1370 627 325 217 125 82
BanII G_rGCy'C
Cuts at: 1391 2052 2501 3170 3362 1391
Size: 661 449 669 192 4072
Fragments arranged by size:
4072 669 661 449 192
BbeI G_GCGC'C
Cuts at: 3243 3243
Size: 6043
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5072
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2461 2495 4234 4268 1903
Size: 34 524 34 1739 34 3678
Fragments arranged by size:
3678 1739 524 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3935 5145 3935
Size: 1210 4833
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6043
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2295 4654 136
Size: 107 122 71 631 605 623 2359 1525
Fragments arranged by size:
2359 1525 631 623 605 122 107 71
BglII A'GATC_T
Cuts at: 6038 6038
Size: 6043
BlpI GC'TnA_GC
Cuts at: 2312 3143 3460 2312
Size: 831 317 4895
Fragments arranged by size:
4895 831 317
Bme1580I G_kGCm'C
Cuts at: 1790 2028 2640 3212 4104 5260 1790
Size: 238 612 572 892 1156 2573
Fragments arranged by size:
2573 1156 892 612 572 238
BmgBI CAC'GTC
Cuts at: 1318 2034 1318
Size: 716 5327
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2158 2392 3774
Size: 416 53 83 399 24 741 234 1382
Cuts at: 3774 4732 442
Size: 958 1753
Fragments arranged by size:
1753 1382 958 741 416 399 234 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6043
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2515 2515
Size: 6043
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2477 2843 2867 2960 3047 3098 4703 1239
Size: 1238 366 24 93 87 51 1605 2579
Fragments arranged by size:
2579 1605 1238 366 93 87 51 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4168 5033 5331 4168
Size: 865 298 4880
Fragments arranged by size:
4880 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2414 4706 882
Size: 617 915 2292 2219
Fragments arranged by size:
2292 2219 915 617
BsaAI yAC'GTr
Cuts at: 493 1204 3834 493
Size: 711 2630 2702
Fragments arranged by size:
2702 2630 711
BsaBI GATnn'nnATC
Cuts at: 2096 6037 2096
Size: 3941 2102
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2403 2520 3232 3240
Size: 53 83 186 936 870 117 712 8
Cuts at: 3240 3850 4232 4867 275
Size: 610 382 635 1451
Fragments arranged by size:
1451 936 870 712 635 610 382 186
117 83 53 8
BsaWI w'CCGG_w
Cuts at: 2556 4476 4874 5148 5295 5706 2556
Size: 1920 398 274 147 411 2893
Fragments arranged by size:
2893 1920 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2180 2210 2372 2402 1178
Size: 30 165 30 777 30 162 30 4819
Fragments arranged by size:
4819 777 165 162 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2609 2906 3059 3095 1186
Size: 1423 297 153 36 4134
Fragments arranged by size:
4134 1423 297 153 36
BseYI C'CCAG_C
Cuts at: 2510 5246 5742 2510
Size: 2736 496 2811
Fragments arranged by size:
2811 2736 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2201 2681 1163
Size: 573 465 480 4525
Fragments arranged by size:
4525 573 480 465
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2349 2441 3493 4254 5282 1540
Size: 275 186 348 92 1052 761 1028 2301
Fragments arranged by size:
2301 1052 1028 761 348 275 186 92
BsiHKAI G_wGCw'C
Cuts at: 2028 2052 2222 4104 4189 5260 2028
Size: 24 170 1882 85 1071 2811
Fragments arranged by size:
2811 1882 1071 170 85 24
BsmI GAATG_Cn'
Cuts at: 3554 3647 3554
Size: 93 5950
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2052 2222 2501 2640 3170 3212
Size: 399 238 24 170 279 139 530 42
Cuts at: 3212 3362 4104 4189 5260 1391
Size: 150 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 530 399 279 238 170
150 139 85 42 24
BspEI T'CCGG_A
Cuts at: 2556 5706 2556
Size: 3150 2893
BspHI T'CATG_A
Cuts at: 3932 5662 3932
Size: 1730 4313
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3120 3398 844
Size: 2276 278 3489
Fragments arranged by size:
3489 2276 278
BsrBI CCG'CTC
Cuts at: 1394 1823 2437 2906 3930 1394
Size: 429 614 469 1024 3507
Fragments arranged by size:
3507 1024 614 469 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4538 4712 65
Size: 1183 3290 174 1396
Fragments arranged by size:
3290 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3253 4687 4874 1700
Size: 110 1443 1434 187 2869
Fragments arranged by size:
2869 1443 1434 187 110
BsrGI T'GTAC_A
Cuts at: 96 2724 96
Size: 2628 3415
BssHII G'CGCG_C
Cuts at: 1925 2341 1925
Size: 416 5627
BssSI C'ACGA_G
Cuts at: 4103 5115 4103
Size: 1012 5031
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6043
BstBI TT'CG_AA
Cuts at: 4847 4847
Size: 6043
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6043
BstXI CCAn_nnnn'nTGG
Cuts at: 2104 2455 2104
Size: 351 5692
BstYI r'GATC_y
Cuts at: 1972 2553 3373 3740 4125 4142 5594 5680 5692
Size: 581 820 367 385 17 1452 86 12
Cuts at: 5692 6038 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 820 581 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2043 2223 2748 513
Size: 552 939 39 180 525 3808
Fragments arranged by size:
3808 939 552 525 180 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3115 487
Size: 683 56 1889 3415
Fragments arranged by size:
3415 1889 683 56
BtsI GCAGTG_nn'
Cuts at: 3559 4353 4373 3559
Size: 794 20 5229
Fragments arranged by size:
5229 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6043
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6008
DraI TTT'AAA
Cuts at: 3347 3347
Size: 6043
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2525 5050 809
Size: 507 1209 2525 1802
Fragments arranged by size:
2525 1802 1209 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2346 2438 3490
Size: 54 1425 50 275 18 516 92 1052
Cuts at: 3490 4379 6008 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1052 889 516 275 92 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2346 2438 3490 1537
Size: 275 534 92 1052 4090
Fragments arranged by size:
4090 1052 534 275 92
EarI CTCTTCn'nnn_
Cuts at: 1353 2928 2955 3973 1353
Size: 1575 27 1018 3423
Fragments arranged by size:
3423 1575 1018 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3056 4630 5004 5150 387
Size: 1064 138 1467 1574 374 146 1280
Fragments arranged by size:
1574 1467 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2050 2050
Size: 6043
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2141 2189 2354 2477 2507 2608
Size: 393 464 45 48 165 123 30 101
Cuts at: 2608 2822 2843 2867 2960 3047 3098 4106 4703
Size: 214 21 24 93 87 51 1008 597
Cuts at: 4703 5490 5758 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1008 787 597 464 393 268 214
165 123 101 93 87 51 48 45
30 24 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2528 2528
Size: 6043
EcoRI G'AATT_C
Cuts at: 3352 3730 3352
Size: 378 5665
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2380 2984 2380
Size: 604 5439
FspI TGC'GCA
Cuts at: 4549 4549
Size: 6043
HaeII r_GCGC'y
Cuts at: 1678 2703 3243 3326 5190 1678
Size: 1025 540 83 1864 2531
Fragments arranged by size:
2531 1864 1025 540 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2372 2404 3286 3318 4684
Size: 32 135 32 800 32 882 32 1366
Cuts at: 4684 4716 4758 4790 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1366 882 800 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3387 3633 669
Size: 988 281 1449 246 3079
Fragments arranged by size:
3079 1449 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2834 2966 748
Size: 2086 132 3825
Fragments arranged by size:
3825 2086 132
HpaI GTT'AAC
Cuts at: 3633 3633
Size: 6043
KasI G'GCGC_C
Cuts at: 3239 3239
Size: 6043
KpnI G_GTAC'C
Cuts at: 3161 3368 3161
Size: 207 5836
MfeI C'AATT_G
Cuts at: 3642 3642
Size: 6043
MluI A'CGCG_T
Cuts at: 5919 5919
Size: 6043
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2169 2349 5157 5341 1305
Size: 864 180 2808 184 2007
Fragments arranged by size:
2808 2007 864 184 180
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4221
Fragments arranged by size:
4221 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2090 2102 2573 2793 4001 4360 4519
Size: 648 924 12 471 220 1208 359 159
Cuts at: 4519 518
Size: 2042
Fragments arranged by size:
2042 1208 924 648 471 359 220 159
12
MspA1I CmG'CkG
Cuts at: 1606 2012 2119 2316 2821 3058 3263 3459 4136
Size: 406 107 197 505 237 205 196 677
Cuts at: 4136 5284 5529 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 505 406 245 237 205
197 196 107
NaeI GCC'GGC
Cuts at: 3255 3255
Size: 6043
NarI GG'CG_CC
Cuts at: 3240 3240
Size: 6043
NcoI C'CATG_G
Cuts at: 513 1065 2004 2223 513
Size: 552 939 219 4333
Fragments arranged by size:
4333 939 552 219
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6043
NgoMIV G'CCGG_C
Cuts at: 3253 3253
Size: 6043
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6043
NotI GC'GGCC_GC
Cuts at: 3490 3490
Size: 6043
NspI r_CATG'y
Cuts at: 1132 1229 3401 4946 1132
Size: 97 2172 1545 2229
Fragments arranged by size:
2229 2172 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6043
PciI A'CATG_T
Cuts at: 4942 4942
Size: 6043
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6043
PmeI GTTT'AAAC
Cuts at: 3347 3347
Size: 6043
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4112 4144 5625 5657 4112
Size: 32 1481 32 4498
Fragments arranged by size:
4498 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6043
PsiI TTA'TAA
Cuts at: 3613 3613
Size: 6043
PstI C_TGCA'G
Cuts at: 830 3039 3063 3117 3179 3262 3395 830
Size: 2209 24 54 62 83 133 3478
Fragments arranged by size:
3478 2209 133 83 62 54 24
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6043
PvuII CAG'CTG
Cuts at: 2119 2821 3058 3263 2119
Size: 702 237 205 4899
Fragments arranged by size:
4899 702 237 205
SacI G_AGCT'C
Cuts at: 2052 2052
Size: 6043
SalI G'TCGA_C
Cuts at: 1936 3385 1936
Size: 1449 4594
SapI GCTCTTCn'nnn_
Cuts at: 2928 2928
Size: 6043
SbfI CC_TGCA'GG
Cuts at: 3395 3395
Size: 6043
ScaI AGT'ACT
Cuts at: 4291 6003 4291
Size: 1712 4331
SfcI C'TryA_G
Cuts at: 826 1045 3035 3059 3113 3175 3258 3264 3391
Size: 219 1990 24 54 62 83 6 127
Cuts at: 3391 4526 5207 5398 826
Size: 1135 681 191 1471
Fragments arranged by size:
1990 1471 1135 681 219 191 127 83
62 54 24 6
SfoI GGC'GCC
Cuts at: 3241 3241
Size: 6043
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6043
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6043
SmaI CCC'GGG
Cuts at: 1872 3163 3195 3311 3370 1872
Size: 1291 32 116 59 4545
Fragments arranged by size:
4545 1291 116 59 32
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4147 5048 5310 820
Size: 197 926 2204 901 262 1553
Fragments arranged by size:
2204 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3834 493
Size: 3341 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6043
SphI G_CATG'C
Cuts at: 1132 3401 1132
Size: 2269 3774
SrfI GCCC'GGGC
Cuts at: 3195 3195
Size: 6043
SspI AAT'ATT
Cuts at: 5 52 3967 5
Size: 47 3915 2081
Fragments arranged by size:
3915 2081 47
StuI AGG'CCT
Cuts at: 1517 2654 1517
Size: 1137 4906
StyI C'CwwG_G
Cuts at: 513 1065 2004 2223 2406 3153 3482 513
Size: 552 939 219 183 747 329 3074
Fragments arranged by size:
3074 939 747 552 329 219 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4070 4087 4240 4425 4870 325
Size: 1255 2490 17 153 185 445 1498
Fragments arranged by size:
2490 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2673 2724 4289
Size: 274 80 33 51 1443 696 51 1565
Cuts at: 4289 6001 96
Size: 1712 138
Fragments arranged by size:
1712 1565 1443 696 274 138 80 51
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2180 2212 6003 6035 2180
Size: 32 3791 32 2188
Fragments arranged by size:
3791 2188 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6043
XbaI T'CTAG_A
Cuts at: 3379 3379
Size: 6043
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2221 2221
Size: 6043
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6043
XmaI C'CCGG_G
Cuts at: 1870 3161 3193 3309 3368 1870
Size: 1291 32 116 59 4545
Fragments arranged by size:
4545 1291 116 59 32
XmnI GAAnn'nnTTC
Cuts at: 4172 4172
Size: 6043
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2521 3851 276
Size: 53 83 186 936 987 1330 2468
Fragments arranged by size:
2468 1330 987 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I EcoRV FseI
FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SwaI