Restriction map for
pFN21AE5567
(Circular) (Six-base) MAPSORT of: pFN21AE5567.seq Check: 3384 from: 1 to: 6004
pFN21AE5567 6004 bp
With 182 enzymes: SgfI *
January 30, 2014 17:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3814 278
Size: 53 83 186 936 2278 2468
Fragments arranged by size:
2468 2278 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3233 3347 1937
Size: 1296 114 4594
Fragments arranged by size:
4594 1296 114
Acc65I G'GTAC_C
Cuts at: 3032 3325 3032
Size: 293 5711
AclI AA'CG_TT
Cuts at: 1563 4131 4504 1563
Size: 2568 373 3063
Fragments arranged by size:
3063 2568 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2076 2149 2194 3204 4067 5451 5719 1632
Size: 444 73 45 1010 863 1384 268 1917
Fragments arranged by size:
1917 1384 1010 863 444 268 73 45
AfeI AGC'GCT
Cuts at: 2162 2162
Size: 6004
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5807
AflIII A'CryG_T
Cuts at: 1203 4903 5880 1203
Size: 3700 977 1327
Fragments arranged by size:
3700 1327 977
AgeI A'CCGG_T
Cuts at: 4835 4835
Size: 6004
AhdI GACnn_n'nnGTC
Cuts at: 4733 4733
Size: 6004
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6004
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6004
AlwNI CAG_nnn'CTG
Cuts at: 2054 2275 2302 3747 5319 2054
Size: 221 27 1445 1572 2739
Fragments arranged by size:
2739 1572 1445 221 27
ApaI G_GGCC'C
Cuts at: 3246 3262 3246
Size: 16 5988
ApaLI G'TGCA_C
Cuts at: 4061 5217 4061
Size: 1156 4848
ApoI r'AATT_y
Cuts at: 1493 2683 3313 3539 3691 5800 1493
Size: 1190 630 226 152 2109 1697
Fragments arranged by size:
2109 1697 1190 630 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4558 160
Size: 872 3526 1606
Fragments arranged by size:
3526 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
AvaI C'yCGr_G
Cuts at: 1870 1943 3131 3329 1870
Size: 73 1188 198 4545
Fragments arranged by size:
4545 1188 198 73
AvrII C'CTAG_G
Cuts at: 2315 2315
Size: 6004
BamHI G'GATC_C
Cuts at: 2500 3178 3334 3701 2500
Size: 678 156 367 4803
Fragments arranged by size:
4803 678 367 156
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2391 2584 3032 3325 4780
Size: 325 217 627 604 193 448 293 1455
Cuts at: 4780 618
Size: 1842
Fragments arranged by size:
1842 1455 627 604 448 325 293 217
193
BanII G_rGCy'C
Cuts at: 1391 2227 3076 3246 3262 3323 1391
Size: 836 849 170 16 61 4072
Fragments arranged by size:
4072 849 836 170 61 16
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3204 928
Size: 971 1305 3728
Fragments arranged by size:
3728 1305 971
BbvCI CC'TCA_GC
Cuts at: 2243 2243
Size: 6004
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2400 2434 4195 4229 1903
Size: 34 463 34 1761 34 3678
Fragments arranged by size:
3678 1761 463 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3896 5106 3896
Size: 1210 4794
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6004
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2582 4615 136
Size: 107 122 71 631 605 910 2033 1525
Fragments arranged by size:
2033 1525 910 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5999 5999
Size: 6004
BlpI GC'TnA_GC
Cuts at: 3421 3421
Size: 6004
Bme1580I G_kGCm'C
Cuts at: 1790 3246 3262 4065 5221 1790
Size: 1456 16 803 1156 2573
Fragments arranged by size:
2573 1456 1156 803 16
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6004
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2827 3735 4693
Size: 416 53 83 399 24 1410 908 958
Cuts at: 4693 442
Size: 1753
Fragments arranged by size:
1753 1410 958 908 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6004
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2880 2880
Size: 6004
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2297 2312 2767 2981 3077 4664 1239
Size: 1058 15 455 214 96 1587 2579
Fragments arranged by size:
2579 1587 1058 455 214 96 15
Bpu10I CC'TnA_GC
Cuts at: 2243 2733 2933 2243
Size: 490 200 5314
Fragments arranged by size:
5314 490 200
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4129 4994 5292 4129
Size: 865 298 4841
Fragments arranged by size:
4841 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2393 2438 2825 4667 882
Size: 617 894 45 387 1842 2219
Fragments arranged by size:
2219 1842 894 617 387 45
BsaAI yAC'GTr
Cuts at: 493 1204 3795 493
Size: 711 2591 2702
Fragments arranged by size:
2702 2591 711
BsaBI GATnn'nnATC
Cuts at: 2101 5998 2101
Size: 3897 2107
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3811 4193 4828 275
Size: 53 83 186 936 2278 382 635 1451
Fragments arranged by size:
2278 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2883 3003 4437 4835 5109 5256 5667 2883
Size: 120 1434 398 274 147 411 3220
Fragments arranged by size:
3220 1434 411 398 274 147 120
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2601 2631 3099 3129 1178
Size: 30 165 30 1198 30 468 30 4053
Fragments arranged by size:
4053 1198 468 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2378 2843 3065 1186
Size: 1192 465 222 4125
Fragments arranged by size:
4125 1192 465 222
BseYI C'CCAG_C
Cuts at: 3174 3262 5207 5703 3174
Size: 88 1945 496 3475
Fragments arranged by size:
3475 1945 496 88
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2357 1163
Size: 573 621 4810
Fragments arranged by size:
4810 621 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3454 4215 5243 1540
Size: 275 186 1453 761 1028 2301
Fragments arranged by size:
2301 1453 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2702 4065 4150 5221 2702
Size: 1363 85 1071 3485
Fragments arranged by size:
3485 1363 1071 85
BsmI GAATG_Cn'
Cuts at: 3515 3608 3515
Size: 93 5911
BsmBI CGTCTCn'nnnn_
Cuts at: 2264 2264
Size: 6004
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2227 2702 3076 3246 3262 3323 4065
Size: 399 437 475 374 170 16 61 742
Cuts at: 4065 4150 5221 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 475 437 399 374 170
85 61 16
BspEI T'CCGG_A
Cuts at: 2883 5667 2883
Size: 2784 3220
BspHI T'CATG_A
Cuts at: 3893 5623 3893
Size: 1730 4274
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3044 3359 844
Size: 2200 315 3489
Fragments arranged by size:
3489 2200 315
BsrBI CCG'CTC
Cuts at: 1394 1823 3891 1394
Size: 429 2068 3507
Fragments arranged by size:
3507 2068 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2919 2972 4499 4673 65
Size: 1183 1671 53 1527 174 1396
Fragments arranged by size:
1671 1527 1396 1183 174 53
BsrFI r'CCGG_y
Cuts at: 1700 1810 4648 4835 1700
Size: 110 2838 187 2869
Fragments arranged by size:
2869 2838 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6004
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6004
BssSI C'ACGA_G
Cuts at: 2201 2285 4064 5076 2201
Size: 84 1779 1012 3129
Fragments arranged by size:
3129 1779 1012 84
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6004
BstBI TT'CG_AA
Cuts at: 4808 4808
Size: 6004
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6004
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2664 2011
Size: 653 5351
BstYI r'GATC_y
Cuts at: 1972 2253 2271 2500 3178 3334 3701 4086 4103
Size: 281 18 229 678 156 367 385 17
Cuts at: 4103 5555 5641 5653 5999 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 678 385 367 346 281 229
156 86 18 17 12
Bsu36I CC'TnA_GG
Cuts at: 2511 2511
Size: 6004
BtgI C'CryG_G
Cuts at: 513 1065 2004 2125 3114 513
Size: 552 939 121 989 3403
Fragments arranged by size:
3403 989 939 552 121
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5265
Fragments arranged by size:
5265 683 56
BtsI GCAGTG_nn'
Cuts at: 2700 3520 4314 4334 2700
Size: 820 794 20 4370
Fragments arranged by size:
4370 820 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6004
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2577 2612 2762 2797 529
Size: 35 2013 35 150 35 3736
Fragments arranged by size:
3736 2013 150 35 35 35
DraI TTT'AAA
Cuts at: 3308 3308
Size: 6004
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5011 809
Size: 507 3695 1802
Fragments arranged by size:
3695 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3451 4340 5969
Size: 54 1425 50 275 18 1621 889 1629
Cuts at: 5969 8
Size: 43
Fragments arranged by size:
1629 1621 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3451 1537
Size: 275 1639 4090
Fragments arranged by size:
4090 1639 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2076 2436 3934 1353
Size: 723 360 1498 3423
Fragments arranged by size:
3423 1498 723 360
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4591 4965 5111 387
Size: 1064 138 3002 374 146 1280
Fragments arranged by size:
3002 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2076 2149 2194 2297 2312 2767 2981
Size: 393 444 73 45 103 15 455 214
Cuts at: 2981 3077 3204 4067 4664 5451 5719 1239
Size: 96 127 863 597 787 268 1524
Fragments arranged by size:
1524 863 787 597 455 444 393 268
214 127 103 96 73 45 15
EcoO109I rG'GnC_Cy
Cuts at: 2451 3061 3242 3258 3259 2451
Size: 610 181 16 1 5196
Fragments arranged by size:
5196 610 181 16 1
EcoRI G'AATT_C
Cuts at: 3313 3691 3313
Size: 378 5626
FspI TGC'GCA
Cuts at: 4510 4510
Size: 6004
HaeII r_GCGC'y
Cuts at: 1678 2164 5151 1678
Size: 486 2987 2531
Fragments arranged by size:
2987 2531 486
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2114 2146 2504 2536 4645
Size: 32 135 32 542 32 358 32 2109
Cuts at: 4645 4677 4719 4751 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2109 542 358 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3348 3594 669
Size: 988 281 1410 246 3079
Fragments arranged by size:
3079 1410 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6004
HpaI GTT'AAC
Cuts at: 3594 3594
Size: 6004
KpnI G_GTAC'C
Cuts at: 3036 3329 3036
Size: 293 5711
MfeI C'AATT_G
Cuts at: 3603 3603
Size: 6004
MluI A'CGCG_T
Cuts at: 5880 5880
Size: 6004
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5118 5302 1305
Size: 3813 184 2007
Fragments arranged by size:
3813 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4182
Fragments arranged by size:
4182 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2571 2801 3962 4321 4480 518
Size: 648 843 562 230 1161 359 159 2042
Fragments arranged by size:
2042 1161 843 648 562 359 230 159
MspA1I CmG'CkG
Cuts at: 1606 2192 2488 3420 4097 5245 5490 1606
Size: 586 296 932 677 1148 245 2120
Fragments arranged by size:
2120 1148 932 677 586 296 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4513
Fragments arranged by size:
4513 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6004
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6004
NotI GC'GGCC_GC
Cuts at: 3451 3451
Size: 6004
NspI r_CATG'y
Cuts at: 1132 1229 2550 3362 4907 1132
Size: 97 1321 812 1545 2229
Fragments arranged by size:
2229 1545 1321 812 97
PasI CC'CwG_GG
Cuts at: 1348 3238 1348
Size: 1890 4114
PciI A'CATG_T
Cuts at: 4903 4903
Size: 6004
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2112 1108
Size: 1004 5000
PfoI T'CCnGG_A
Cuts at: 2274 2274
Size: 6004
PmeI GTTT'AAAC
Cuts at: 3308 3308
Size: 6004
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4073 4105 5586 5618 4073
Size: 32 1481 32 4459
Fragments arranged by size:
4459 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6004
PsiI TTA'TAA
Cuts at: 2681 3574 2681
Size: 893 5111
PspOMI G'GGCC_C
Cuts at: 3242 3258 3242
Size: 16 5988
PstI C_TGCA'G
Cuts at: 830 2413 2493 2707 3356 830
Size: 1583 80 214 649 3478
Fragments arranged by size:
3478 1583 649 214 80
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6004
PvuII CAG'CTG
Cuts at: 2192 2488 2192
Size: 296 5708
SalI G'TCGA_C
Cuts at: 1936 3346 1936
Size: 1410 4594
SbfI CC_TGCA'GG
Cuts at: 3356 3356
Size: 6004
ScaI AGT'ACT
Cuts at: 4252 5964 4252
Size: 1712 4292
SexAI A'CCwGG_T
Cuts at: 3028 3028
Size: 6004
SfcI C'TryA_G
Cuts at: 826 1045 2409 2489 2703 2974 3352 4487 5168
Size: 219 1364 80 214 271 378 1135 681
Cuts at: 5168 5359 826
Size: 191 1471
Fragments arranged by size:
1471 1364 1135 681 378 271 219 214
191 80
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6004
SmaI CCC'GGG
Cuts at: 1872 3331 1872
Size: 1459 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4108 5009 5271 820
Size: 197 926 2165 901 262 1553
Fragments arranged by size:
2165 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3795 493
Size: 3302 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6004
SphI G_CATG'C
Cuts at: 1132 3362 1132
Size: 2230 3774
SspI AAT'ATT
Cuts at: 5 52 3928 5
Size: 47 3876 2081
Fragments arranged by size:
3876 2081 47
StuI AGG'CCT
Cuts at: 1517 2150 2207 1517
Size: 633 57 5314
Fragments arranged by size:
5314 633 57
StyI C'CwwG_G
Cuts at: 513 1065 2004 2315 2376 3443 513
Size: 552 939 311 61 1067 3074
Fragments arranged by size:
3074 1067 939 552 311 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2323 3039 4031 4048 4201 4386 4831
Size: 1255 743 716 992 17 153 185 445
Cuts at: 4831 325
Size: 1498
Fragments arranged by size:
1498 1255 992 743 716 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4250 5962 96
Size: 274 80 33 51 1443 2273 1712 138
Fragments arranged by size:
2273 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3099 3131 5964 5996 3099
Size: 32 2833 32 3107
Fragments arranged by size:
3107 2833 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6004
XbaI T'CTAG_A
Cuts at: 3340 3340
Size: 6004
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2354 2502 2842 3281 2354
Size: 148 340 439 5077
Fragments arranged by size:
5077 439 340 148
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6004
XmaI C'CCGG_G
Cuts at: 1870 3329 1870
Size: 1459 4545
XmnI GAAnn'nnTTC
Cuts at: 4133 4133
Size: 6004
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3812 276
Size: 53 83 186 936 2278 2468
Fragments arranged by size:
2468 2278 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BbeI BfrBI BsiWI BstZ17I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspXI PsrI RsrII SacI SacII SanDI SapI SfiI
SfoI SrfI SwaI