Restriction map for
pFN21AE5564
(Circular) (Six-base) MAPSORT of: pFN21AE5564.seq Check: 8386 from: 1 to: 5977
pFN21AE5564 5977 bp
With 182 enzymes: SgfI *
January 30, 2014 17:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2612 2612
Size: 5977
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2724 3787 278
Size: 53 83 186 936 1188 1063 2468
Fragments arranged by size:
2468 1188 1063 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2355 3320 1937
Size: 418 965 4594
Fragments arranged by size:
4594 965 418
Acc65I G'GTAC_C
Cuts at: 3298 3298
Size: 5977
AclI AA'CG_TT
Cuts at: 1563 4104 4477 1563
Size: 2541 373 3063
Fragments arranged by size:
3063 2541 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2708 4040 5424 5692 1632
Size: 1076 1332 1384 268 1917
Fragments arranged by size:
1917 1384 1332 1076 268
AfeI AGC'GCT
Cuts at: 2059 2388 2059
Size: 329 5648
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5780
AflIII A'CryG_T
Cuts at: 1203 4876 5853 1203
Size: 3673 977 1327
Fragments arranged by size:
3673 1327 977
AgeI A'CCGG_T
Cuts at: 4808 4808
Size: 5977
AhdI GACnn_n'nnGTC
Cuts at: 3149 4706 3149
Size: 1557 4420
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5977
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5977
AlwNI CAG_nnn'CTG
Cuts at: 2224 2881 3030 3720 5292 2224
Size: 657 149 690 1572 2909
Fragments arranged by size:
2909 1572 690 657 149
ApaLI G'TGCA_C
Cuts at: 4034 5190 4034
Size: 1156 4821
ApoI r'AATT_y
Cuts at: 1493 3286 3512 3664 5773 1493
Size: 1793 226 152 2109 1697
Fragments arranged by size:
2109 1793 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4531 160
Size: 872 3499 1606
Fragments arranged by size:
3499 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
AvaI C'yCGr_G
Cuts at: 1870 1943 2016 2348 2441 2667 2725 3302 1870
Size: 73 73 332 93 226 58 577 4545
Fragments arranged by size:
4545 577 332 226 93 73 73 58
BamHI G'GATC_C
Cuts at: 3307 3674 3307
Size: 367 5610
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2437 3007 3298 4753 618
Size: 325 217 627 650 570 291 1455 1842
Fragments arranged by size:
1842 1455 650 627 570 325 291 217
BanII G_rGCy'C
Cuts at: 1391 2017 2023 2772 2859 3296 1391
Size: 626 6 749 87 437 4072
Fragments arranged by size:
4072 749 626 437 87 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2938 2980 3181 928
Size: 971 1039 42 201 3724
Fragments arranged by size:
3724 1039 971 201 42
BbvCI CC'TCA_GC
Cuts at: 2594 2594
Size: 5977
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 1997 2021 2031 4168 4202 1903
Size: 34 50 10 24 10 2137 34 3678
Fragments arranged by size:
3678 2137 50 34 34 24 10 10
BciVI GTATCCnnnnn_n'
Cuts at: 3869 5079 3869
Size: 1210 4767
BclI T'GATC_A
Cuts at: 1548 3063 1548
Size: 1515 4462
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2472 2561 4588
Size: 107 122 71 631 605 800 89 2027
Cuts at: 4588 136
Size: 1525
Fragments arranged by size:
2027 1525 800 631 605 122 107 89
71
BglII A'GATC_T
Cuts at: 5972 5972
Size: 5977
BlpI GC'TnA_GC
Cuts at: 2897 3394 2897
Size: 497 5480
Bme1580I G_kGCm'C
Cuts at: 1790 2552 3012 4038 5194 1790
Size: 762 460 1026 1156 2573
Fragments arranged by size:
2573 1156 1026 762 460
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5977
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2174 3708 4666
Size: 416 53 83 399 24 757 1534 958
Cuts at: 4666 442
Size: 1753
Fragments arranged by size:
1753 1534 958 757 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5977
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2516 2609 2516
Size: 93 5884
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2317 2837 2846 3259 4637 1239
Size: 1078 520 9 413 1378 2579
Fragments arranged by size:
2579 1378 1078 520 413 9
Bpu10I CC'TnA_GC
Cuts at: 2594 2594
Size: 5977
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4102 4967 5265 4102
Size: 865 298 4814
Fragments arranged by size:
4814 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2533 4640 882
Size: 617 1034 2107 2219
Fragments arranged by size:
2219 2107 1034 617
BsaAI yAC'GTr
Cuts at: 493 1204 2095 3199 3768 493
Size: 711 891 1104 569 2702
Fragments arranged by size:
2702 1104 891 711 569
BsaBI GATnn'nnATC
Cuts at: 2420 5971 2420
Size: 3551 2426
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2487 2640 2721 3180
Size: 53 83 186 936 954 153 81 459
Cuts at: 3180 3784 4166 4801 275
Size: 604 382 635 1451
Fragments arranged by size:
1451 954 936 635 604 459 382 186
153 83 81 53
BsaWI w'CCGG_w
Cuts at: 4410 4808 5082 5229 5640 4410
Size: 398 274 147 411 4747
Fragments arranged by size:
4747 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3114 3144 1178
Size: 30 165 30 1711 30 4011
Fragments arranged by size:
4011 1711 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2459 2849 2930 1186
Size: 1273 390 81 4233
Fragments arranged by size:
4233 1273 390 81
BseYI C'CCAG_C
Cuts at: 2224 2470 2512 3100 5180 5676 2224
Size: 246 42 588 2080 496 2525
Fragments arranged by size:
2525 2080 588 496 246 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3016 3221 1163
Size: 573 1280 205 3919
Fragments arranged by size:
3919 1280 573 205
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2328 3153 3427 4188 5216 1540
Size: 275 186 327 825 274 761 1028 2301
Fragments arranged by size:
2301 1028 825 761 327 275 274 186
BsiHKAI G_wGCw'C
Cuts at: 2017 4038 4123 5194 2017
Size: 2021 85 1071 2800
Fragments arranged by size:
2800 2021 1071 85
BsiWI C'GTAC_G
Cuts at: 2535 2535
Size: 5977
BsmI GAATG_Cn'
Cuts at: 3488 3581 3488
Size: 93 5884
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2017 2023 2552 2772 2859 3012 3296
Size: 399 227 6 529 220 87 153 284
Cuts at: 3296 4038 4123 5194 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 529 399 284 227 220
153 87 85 6
BspEI T'CCGG_A
Cuts at: 5640 5640
Size: 5977
BspHI T'CATG_A
Cuts at: 3866 5596 3866
Size: 1730 4247
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2126 2202 2235 2322 2367 2612 3193 3332
Size: 1282 76 33 87 45 245 581 139
Cuts at: 3332 844
Size: 3489
Fragments arranged by size:
3489 1282 581 245 139 87 76 45
33
BsrBI CCG'CTC
Cuts at: 1394 1823 2449 3864 1394
Size: 429 626 1415 3507
Fragments arranged by size:
3507 1415 626 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2178 4472 4646 65
Size: 1183 930 2294 174 1396
Fragments arranged by size:
2294 1396 1183 930 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2067 2233 2466 4621 4808 1700
Size: 110 257 166 233 2155 187 2869
Fragments arranged by size:
2869 2155 257 233 187 166 110
BsrGI T'GTAC_A
Cuts at: 96 3118 96
Size: 3022 2955
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5977
BssSI C'ACGA_G
Cuts at: 4037 5049 4037
Size: 1012 4965
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2401 794
Size: 1607 4370
BstBI TT'CG_AA
Cuts at: 4781 4781
Size: 5977
BstEII G'GTnAC_C
Cuts at: 1184 2869 3046 1184
Size: 1685 177 4115
Fragments arranged by size:
4115 1685 177
BstXI CCAn_nnnn'nTGG
Cuts at: 2581 2941 2581
Size: 360 5617
BstYI r'GATC_y
Cuts at: 1972 2730 3307 3674 4059 4076 5528 5614 5626
Size: 758 577 367 385 17 1452 86 12
Cuts at: 5626 5972 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 758 577 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2454 2799 2967 3147 513
Size: 552 1389 345 168 180 3343
Fragments arranged by size:
3343 1389 552 345 180 168
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2680 487
Size: 683 56 1454 3784
Fragments arranged by size:
3784 1454 683 56
BtsI GCAGTG_nn'
Cuts at: 2651 3125 3493 4287 4307 2651
Size: 474 368 794 20 4321
Fragments arranged by size:
4321 794 474 368 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5977
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5942
DraI TTT'AAA
Cuts at: 3281 3281
Size: 5977
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4984 809
Size: 507 3668 1802
Fragments arranged by size:
3668 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2100 2325 2553
Size: 54 1425 50 275 18 270 225 228
Cuts at: 2553 2571 2964 3193 3424 4313 5942 8
Size: 18 393 229 231 889 1629 43
Fragments arranged by size:
1629 1425 889 393 275 270 231 229
228 225 54 50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2325 3424 1537
Size: 275 513 1099 4090
Fragments arranged by size:
4090 1099 513 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2129 2369 2777 3907 1353
Size: 776 240 408 1130 3423
Fragments arranged by size:
3423 1130 776 408 240
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4564 4938 5084 387
Size: 1064 138 2975 374 146 1280
Fragments arranged by size:
2975 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2015 2015
Size: 5977
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2317 2708 2837 2846 3259 4040 4637
Size: 393 685 391 129 9 413 781 597
Cuts at: 4637 5424 5692 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 781 685 597 413 393 391
268 129 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2741 2741
Size: 5977
EcoO109I rG'GnC_Cy
Cuts at: 2520 2878 3143 2520
Size: 358 265 5354
Fragments arranged by size:
5354 358 265
EcoRI G'AATT_C
Cuts at: 3286 3664 3286
Size: 378 5599
FspI TGC'GCA
Cuts at: 4483 4483
Size: 5977
HaeII r_GCGC'y
Cuts at: 1678 2061 2279 2309 2390 5124 1678
Size: 383 218 30 81 2734 2531
Fragments arranged by size:
2734 2531 383 218 81 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2044 2076 3135 3167 4618
Size: 32 135 32 472 32 1059 32 1451
Cuts at: 4618 4650 4692 4724 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1451 1059 472 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3321 3567 669
Size: 988 281 1383 246 3079
Fragments arranged by size:
3079 1383 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5977
HpaI GTT'AAC
Cuts at: 3567 3567
Size: 5977
KpnI G_GTAC'C
Cuts at: 3302 3302
Size: 5977
MfeI C'AATT_G
Cuts at: 3576 3576
Size: 5977
MluI A'CGCG_T
Cuts at: 5853 5853
Size: 5977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2470 2778 5091 5275 1305
Size: 1165 308 2313 184 2007
Fragments arranged by size:
2313 2007 1165 308 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2573 2966 10
Size: 54 1425 343 741 393 3021
Fragments arranged by size:
3021 1425 741 393 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2780 2798 2954 3935 4294 4453 518
Size: 648 1614 18 156 981 359 159 2042
Fragments arranged by size:
2042 1614 981 648 359 159 156 18
MspA1I CmG'CkG
Cuts at: 1606 2221 2456 2815 2896 3393 4070 5218 5463
Size: 615 235 359 81 497 677 1148 245
Cuts at: 5463 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 615 497 359 245 235
81
NaeI GCC'GGC
Cuts at: 2468 2468
Size: 5977
NcoI C'CATG_G
Cuts at: 513 1065 2799 2967 513
Size: 552 1734 168 3523
Fragments arranged by size:
3523 1734 552 168
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5977
NgoMIV G'CCGG_C
Cuts at: 2466 2466
Size: 5977
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5977
NotI GC'GGCC_GC
Cuts at: 2325 3424 2325
Size: 1099 4878
NspI r_CATG'y
Cuts at: 1132 1229 3061 3335 4880 1132
Size: 97 1832 274 1545 2229
Fragments arranged by size:
2229 1832 1545 274 97
PasI CC'CwG_GG
Cuts at: 1348 2025 2461 2875 1348
Size: 677 436 414 4450
Fragments arranged by size:
4450 677 436 414
PciI A'CATG_T
Cuts at: 4876 4876
Size: 5977
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2881 2973 1108
Size: 1773 92 4112
Fragments arranged by size:
4112 1773 92
PmeI GTTT'AAAC
Cuts at: 3281 3281
Size: 5977
PmlI CAC'GTG
Cuts at: 3199 3199
Size: 5977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4046 4078 5559 5591 4046
Size: 32 1481 32 4432
Fragments arranged by size:
4432 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2520 3143 2520
Size: 623 5354
PshAI GACnn'nnGTC
Cuts at: 1613 2525 1613
Size: 912 5065
PsiI TTA'TAA
Cuts at: 3547 3547
Size: 5977
PstI C_TGCA'G
Cuts at: 830 2220 3329 830
Size: 1390 1109 3478
Fragments arranged by size:
3478 1390 1109
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5977
PvuII CAG'CTG
Cuts at: 2221 2815 2896 2221
Size: 594 81 5302
Fragments arranged by size:
5302 594 81
SacI G_AGCT'C
Cuts at: 2017 2017
Size: 5977
SacII CC_GC'GG
Cuts at: 2457 2457
Size: 5977
SalI G'TCGA_C
Cuts at: 1936 3319 1936
Size: 1383 4594
SanDI GG'GwC_CC
Cuts at: 2520 3143 2520
Size: 623 5354
SapI GCTCTTCn'nnn_
Cuts at: 2369 2777 2369
Size: 408 5569
SbfI CC_TGCA'GG
Cuts at: 3329 3329
Size: 5977
ScaI AGT'ACT
Cuts at: 4225 5937 4225
Size: 1712 4265
SfcI C'TryA_G
Cuts at: 826 1045 2216 3325 4460 5141 5332 826
Size: 219 1171 1109 1135 681 191 1471
Fragments arranged by size:
1471 1171 1135 1109 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2472 2472
Size: 5977
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5977
SmaI CCC'GGG
Cuts at: 1872 2350 2669 3304 1872
Size: 478 319 635 4545
Fragments arranged by size:
4545 635 478 319
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4081 4982 5244 820
Size: 197 926 2138 901 262 1553
Fragments arranged by size:
2138 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3768 493
Size: 3275 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5977
SphI G_CATG'C
Cuts at: 1132 3061 3335 1132
Size: 1929 274 3774
Fragments arranged by size:
3774 1929 274
SspI AAT'ATT
Cuts at: 5 52 3901 5
Size: 47 3849 2081
Fragments arranged by size:
3849 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5977
StyI C'CwwG_G
Cuts at: 513 1065 2301 2799 2967 3416 513
Size: 552 1236 498 168 449 3074
Fragments arranged by size:
3074 1236 552 498 449 168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2065 4004 4021 4174 4359 4804 325
Size: 1255 485 1939 17 153 185 445 1498
Fragments arranged by size:
1939 1498 1255 485 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3118 4223 5935
Size: 274 80 33 51 1443 1141 1105 1712
Cuts at: 5935 96
Size: 138
Fragments arranged by size:
1712 1443 1141 1105 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5937 5969 5937
Size: 32 5945
Tth111I GACn'n_nGTC
Cuts at: 1316 3269 1316
Size: 1953 4024
XbaI T'CTAG_A
Cuts at: 3313 3313
Size: 5977
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2797 2797
Size: 5977
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5977
XmaI C'CCGG_G
Cuts at: 1870 2348 2667 3302 1870
Size: 478 319 635 4545
Fragments arranged by size:
4545 635 478 319
XmnI GAAnn'nnTTC
Cuts at: 4106 4106
Size: 5977
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2722 3785 276
Size: 53 83 186 936 1188 1063 2468
Fragments arranged by size:
2468 1188 1063 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BfrBI BsmBI
BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PfoI PspOMI PspXI PsrI
RsrII SexAI SfoI SrfI SwaI