Restriction map for
pFN21AE5563
(Circular) (Six-base) MAPSORT of: pFN21AE5563.seq Check: 4027 from: 1 to: 5941
pFN21AE5563 5941 bp
With 182 enzymes: SgfI *
December 8, 2014 13:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2427 3751 278
Size: 53 83 186 936 891 1324 2468
Fragments arranged by size:
2468 1324 936 891 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3284 1937
Size: 1347 4594
Acc65I G'GTAC_C
Cuts at: 3262 3262
Size: 5941
AclI AA'CG_TT
Cuts at: 1563 4068 4441 1563
Size: 2505 373 3063
Fragments arranged by size:
3063 2505 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4004 5388 5656 1632
Size: 2372 1384 268 1917
Fragments arranged by size:
2372 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2044 820
Size: 197 1027 4717
Fragments arranged by size:
4717 1027 197
AflIII A'CryG_T
Cuts at: 1203 2896 4840 5817 1203
Size: 1693 1944 977 1327
Fragments arranged by size:
1944 1693 1327 977
AgeI A'CCGG_T
Cuts at: 4772 4772
Size: 5941
AhdI GACnn_n'nnGTC
Cuts at: 4670 4670
Size: 5941
AleI CACnn'nnGTG
Cuts at: 1166 3033 1166
Size: 1867 4074
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5941
AlwNI CAG_nnn'CTG
Cuts at: 3233 3684 5256 3233
Size: 451 1572 3918
Fragments arranged by size:
3918 1572 451
ApaI G_GGCC'C
Cuts at: 2672 2672
Size: 5941
ApaLI G'TGCA_C
Cuts at: 3188 3998 5154 3188
Size: 810 1156 3975
Fragments arranged by size:
3975 1156 810
ApoI r'AATT_y
Cuts at: 1493 2397 2456 3182 3250 3476 3628 5737 1493
Size: 904 59 726 68 226 152 2109 1697
Fragments arranged by size:
2109 1697 904 726 226 152 68 59
AseI AT'TA_AT
Cuts at: 160 1032 2610 4495 160
Size: 872 1578 1885 1606
Fragments arranged by size:
1885 1606 1578 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
AvaI C'yCGr_G
Cuts at: 1870 1943 3266 1870
Size: 73 1323 4545
Fragments arranged by size:
4545 1323 73
BamHI G'GATC_C
Cuts at: 2485 3271 3638 2485
Size: 786 367 4788
Fragments arranged by size:
4788 786 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3262 4717 618
Size: 325 217 627 1475 1455 1842
Fragments arranged by size:
1842 1475 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2672 3260 1391
Size: 1281 588 4072
Fragments arranged by size:
4072 1281 588
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2724 2994 928
Size: 971 825 270 3875
Fragments arranged by size:
3875 971 825 270
BbvCI CC'TCA_GC
Cuts at: 2417 2555 2417
Size: 138 5803
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4132 4166 1903
Size: 34 2195 34 3678
Fragments arranged by size:
3678 2195 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3833 5043 3833
Size: 1210 4731
BclI T'GATC_A
Cuts at: 1548 2676 2739 2929 1548
Size: 1128 63 190 4560
Fragments arranged by size:
4560 1128 190 63
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4552 136
Size: 107 122 71 631 605 2880 1525
Fragments arranged by size:
2880 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5936 5936
Size: 5941
BlpI GC'TnA_GC
Cuts at: 3358 3358
Size: 5941
Bme1580I G_kGCm'C
Cuts at: 1790 2672 2953 3192 4002 5158 1790
Size: 882 281 239 810 1156 2573
Fragments arranged by size:
2573 1156 882 810 281 239
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5941
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3672 4630 442
Size: 416 53 83 399 24 2255 958 1753
Fragments arranged by size:
2255 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2925 4601 1239
Size: 1686 1676 2579
Fragments arranged by size:
2579 1686 1676
Bpu10I CC'TnA_GC
Cuts at: 2145 2417 2555 2145
Size: 272 138 5531
Fragments arranged by size:
5531 272 138
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2169 4066 4931 5229 2169
Size: 1897 865 298 2881
Fragments arranged by size:
2881 1897 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2415 4604 882
Size: 617 916 2189 2219
Fragments arranged by size:
2219 2189 916 617
BsaAI yAC'GTr
Cuts at: 493 1204 3732 493
Size: 711 2528 2702
Fragments arranged by size:
2702 2528 711
BsaBI GATnn'nnATC
Cuts at: 5935 5935
Size: 5941
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2424 3748 4130 4765
Size: 53 83 186 936 891 1324 382 635
Cuts at: 4765 275
Size: 1451
Fragments arranged by size:
1451 1324 936 891 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4374 4772 5046 5193 5604 4374
Size: 398 274 147 411 4711
Fragments arranged by size:
4711 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2430 2460 1178
Size: 30 165 30 1027 30 4659
Fragments arranged by size:
4659 1027 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5941
BseYI C'CCAG_C
Cuts at: 2952 5144 5640 2952
Size: 2192 496 3253
Fragments arranged by size:
3253 2192 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3057 1163
Size: 573 1321 4047
Fragments arranged by size:
4047 1321 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3391 4152 5180 1540
Size: 275 186 1390 761 1028 2301
Fragments arranged by size:
2301 1390 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2200 2603 3192 4002 4087 5158 2200
Size: 403 589 810 85 1071 2983
Fragments arranged by size:
2983 1071 810 589 403 85
BsmI GAATG_Cn'
Cuts at: 3452 3545 3452
Size: 93 5848
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2200 2603 2672 2953 3192 3260 4002
Size: 399 410 403 69 281 239 68 742
Cuts at: 4002 4087 5158 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 410 403 399 281 239
85 69 68
BspEI T'CCGG_A
Cuts at: 5604 5604
Size: 5941
BspHI T'CATG_A
Cuts at: 3830 5560 3830
Size: 1730 4211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3296 844
Size: 2452 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3828 1394
Size: 429 2005 3507
Fragments arranged by size:
3507 2005 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2376 2655 4436 4610 65
Size: 1183 1128 279 1781 174 1396
Fragments arranged by size:
1781 1396 1183 1128 279 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4585 4772 1700
Size: 110 2775 187 2869
Fragments arranged by size:
2869 2775 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5941
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5941
BssSI C'ACGA_G
Cuts at: 4001 5013 4001
Size: 1012 4929
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3129 794
Size: 2335 3606
BstBI TT'CG_AA
Cuts at: 2860 4745 2860
Size: 1885 4056
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5941
BstYI r'GATC_y
Cuts at: 1972 2073 2485 3271 3638 4023 4040 5492 5578
Size: 101 412 786 367 385 17 1452 86
Cuts at: 5578 5590 5936 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 786 412 385 367 346 101
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2354 2685 513
Size: 552 1289 331 3769
Fragments arranged by size:
3769 1289 552 331
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5202
Fragments arranged by size:
5202 683 56
BtsI GCAGTG_nn'
Cuts at: 3028 3040 3457 4251 4271 3028
Size: 12 417 794 20 4698
Fragments arranged by size:
4698 794 417 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5941
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5906
DraI TTT'AAA
Cuts at: 3245 3245
Size: 5941
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4948 809
Size: 507 3632 1802
Fragments arranged by size:
3632 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2783 3388 4277
Size: 54 1425 50 275 18 953 605 889
Cuts at: 4277 5906 8
Size: 1629 43
Fragments arranged by size:
1629 1425 953 889 605 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3388 1537
Size: 275 1576 4090
Fragments arranged by size:
4090 1576 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2272 3871 1353
Size: 919 1599 3423
Fragments arranged by size:
3423 1599 919
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4528 4902 5048 387
Size: 1064 138 2939 374 146 1280
Fragments arranged by size:
2939 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2925 4004 4601 5388 5656 1239
Size: 393 1293 1079 597 787 268 1524
Fragments arranged by size:
1524 1293 1079 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2152 2668 2669 3014 2152
Size: 516 1 345 5079
Fragments arranged by size:
5079 516 345 1
EcoRI G'AATT_C
Cuts at: 2397 2456 3250 3628 2397
Size: 59 794 378 4710
Fragments arranged by size:
4710 794 378 59
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3011 3011
Size: 5941
FspI TGC'GCA
Cuts at: 4447 4447
Size: 5941
HaeII r_GCGC'y
Cuts at: 1678 5088 1678
Size: 3410 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4582 4614 4656 4688 1373
Size: 32 135 32 3010 32 42 32 2626
Fragments arranged by size:
3010 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3086 3285 3531 669
Size: 988 281 1148 199 246 3079
Fragments arranged by size:
3079 1148 988 281 246 199
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5941
HpaI GTT'AAC
Cuts at: 3531 3531
Size: 5941
KpnI G_GTAC'C
Cuts at: 3266 3266
Size: 5941
MfeI C'AATT_G
Cuts at: 3540 3540
Size: 5941
MluI A'CGCG_T
Cuts at: 5817 5817
Size: 5941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2164 2310 5055 5239 1305
Size: 859 146 2745 184 2007
Fragments arranged by size:
2745 2007 859 184 146
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4119
Fragments arranged by size:
4119 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3033 3899 4258 4417 518
Size: 648 1867 866 359 159 2042
Fragments arranged by size:
2042 1867 866 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2416 3091 3357 4034 5182 5427 1606
Size: 810 675 266 677 1148 245 2120
Fragments arranged by size:
2120 1148 810 677 675 266 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5389
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5941
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5941
NotI GC'GGCC_GC
Cuts at: 3388 3388
Size: 5941
NspI r_CATG'y
Cuts at: 1132 1229 2900 3299 4844 1132
Size: 97 1671 399 1545 2229
Fragments arranged by size:
2229 1671 1545 399 97
PasI CC'CwG_GG
Cuts at: 1348 3004 1348
Size: 1656 4285
PciI A'CATG_T
Cuts at: 2896 4840 2896
Size: 1944 3997
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2134 1108
Size: 1026 4915
PmeI GTTT'AAAC
Cuts at: 3245 3245
Size: 5941
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4010 4042 5523 5555 4010
Size: 32 1481 32 4396
Fragments arranged by size:
4396 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5941
PsiI TTA'TAA
Cuts at: 3511 3511
Size: 5941
PspOMI G'GGCC_C
Cuts at: 2668 2668
Size: 5941
PstI C_TGCA'G
Cuts at: 830 2385 3035 3293 830
Size: 1555 650 258 3478
Fragments arranged by size:
3478 1555 650 258
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5941
PvuII CAG'CTG
Cuts at: 2416 3091 2416
Size: 675 5266
SalI G'TCGA_C
Cuts at: 1936 3283 1936
Size: 1347 4594
SbfI CC_TGCA'GG
Cuts at: 3293 3293
Size: 5941
ScaI AGT'ACT
Cuts at: 4189 5901 4189
Size: 1712 4229
SfcI C'TryA_G
Cuts at: 826 1045 2381 2632 2963 3031 3289 4424 5105
Size: 219 1336 251 331 68 258 1135 681
Cuts at: 5105 5296 826
Size: 191 1471
Fragments arranged by size:
1471 1336 1135 681 331 258 251 219
191 68
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5941
SmaI CCC'GGG
Cuts at: 1872 3268 1872
Size: 1396 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2044 2184 4045 4946 5208 820
Size: 197 926 101 140 1861 901 262 1553
Fragments arranged by size:
1861 1553 926 901 262 197 140 101
SnaBI TAC'GTA
Cuts at: 493 3732 493
Size: 3239 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5941
SphI G_CATG'C
Cuts at: 1132 3299 1132
Size: 2167 3774
SspI AAT'ATT
Cuts at: 5 52 3865 5
Size: 47 3813 2081
Fragments arranged by size:
3813 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5941
StyI C'CwwG_G
Cuts at: 513 1065 3380 513
Size: 552 2315 3074
Fragments arranged by size:
3074 2315 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2906 3968 3985 4138 4323 4768 325
Size: 1255 1326 1062 17 153 185 445 1498
Fragments arranged by size:
1498 1326 1255 1062 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4187 5899 96
Size: 274 80 33 51 1443 2210 1712 138
Fragments arranged by size:
2210 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2430 2462 5901 5933 2430
Size: 32 3439 32 2438
Fragments arranged by size:
3439 2438 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5941
XbaI T'CTAG_A
Cuts at: 3277 3277
Size: 5941
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5941
XmaI C'CCGG_G
Cuts at: 1870 3266 1870
Size: 1396 4545
XmnI GAAnn'nnTTC
Cuts at: 2031 2651 4070 2031
Size: 620 1419 3902
Fragments arranged by size:
3902 1419 620
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2425 3749 276
Size: 53 83 186 936 891 1324 2468
Fragments arranged by size:
2468 1324 936 891 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI