Restriction map for
pFN21AE5562
(Circular) (Six-base) MAPSORT of: pFN21AE5562.seq Check: 4921 from: 1 to: 5941
pFN21AE5562 5941 bp
With 182 enzymes: SgfI *
January 30, 2014 17:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3751 278
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3284 1937
Size: 1347 4594
Acc65I G'GTAC_C
Cuts at: 2602 3262 2602
Size: 660 5281
AclI AA'CG_TT
Cuts at: 1563 4068 4441 1563
Size: 2505 373 3063
Fragments arranged by size:
3063 2505 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2252 4004 5388 5656 1632
Size: 620 1752 1384 268 1917
Fragments arranged by size:
1917 1752 1384 620 268
AfeI AGC'GCT
Cuts at: 2096 2096
Size: 5941
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5744
AflIII A'CryG_T
Cuts at: 1203 2121 4840 5817 1203
Size: 918 2719 977 1327
Fragments arranged by size:
2719 1327 977 918
AgeI A'CCGG_T
Cuts at: 4772 4772
Size: 5941
AhdI GACnn_n'nnGTC
Cuts at: 4670 4670
Size: 5941
AleI CACnn'nnGTG
Cuts at: 1166 2120 1166
Size: 954 4987
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5941
AlwNI CAG_nnn'CTG
Cuts at: 2155 3684 5256 2155
Size: 1529 1572 2840
Fragments arranged by size:
2840 1572 1529
ApaLI G'TGCA_C
Cuts at: 2918 3998 5154 2918
Size: 1080 1156 3705
Fragments arranged by size:
3705 1156 1080
ApoI r'AATT_y
Cuts at: 1493 2131 3250 3476 3628 5737 1493
Size: 638 1119 226 152 2109 1697
Fragments arranged by size:
2109 1697 1119 638 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4495 160
Size: 872 3463 1606
Fragments arranged by size:
3463 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
AvaI C'yCGr_G
Cuts at: 1870 1943 2777 3014 3266 1870
Size: 73 834 237 252 4545
Fragments arranged by size:
4545 834 252 237 73
BamHI G'GATC_C
Cuts at: 3271 3638 3271
Size: 367 5574
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2602 2857 3262 4717 618
Size: 325 217 627 815 255 405 1455 1842
Fragments arranged by size:
1842 1455 815 627 405 325 255 217
BanII G_rGCy'C
Cuts at: 1391 2382 3260 1391
Size: 991 878 4072
Fragments arranged by size:
4072 991 878
BbeI G_GCGC'C
Cuts at: 2861 2861
Size: 5941
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2548 3157 928
Size: 971 649 609 3712
Fragments arranged by size:
3712 971 649 609
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4132 4166 1903
Size: 34 50 34 2111 34 3678
Fragments arranged by size:
3678 2111 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3833 5043 3833
Size: 1210 4731
BclI T'GATC_A
Cuts at: 1548 2637 1548
Size: 1089 4852
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2913 4552 136
Size: 107 122 71 631 605 1241 1639 1525
Fragments arranged by size:
1639 1525 1241 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5936 5936
Size: 5941
BlpI GC'TnA_GC
Cuts at: 3358 3358
Size: 5941
Bme1580I G_kGCm'C
Cuts at: 1790 2922 4002 5158 1790
Size: 1132 1080 1156 2573
Fragments arranged by size:
2573 1156 1132 1080
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5941
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2611 3672 4630
Size: 416 53 83 399 24 1194 1061 958
Cuts at: 4630 442
Size: 1753
Fragments arranged by size:
1753 1194 1061 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2145 3065 4601 1239
Size: 906 920 1536 2579
Fragments arranged by size:
2579 1536 920 906
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4066 4931 5229 4066
Size: 865 298 4778
Fragments arranged by size:
4778 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4604 882
Size: 617 3105 2219
Fragments arranged by size:
3105 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3732 493
Size: 711 2528 2702
Fragments arranged by size:
2702 2528 711
BsaBI GATnn'nnATC
Cuts at: 5935 5935
Size: 5941
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2103 2858 3748 4130
Size: 53 83 186 936 570 755 890 382
Cuts at: 4130 4765 275
Size: 635 1451
Fragments arranged by size:
1451 936 890 755 635 570 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4374 4772 5046 5193 5604 4374
Size: 398 274 147 411 4711
Fragments arranged by size:
4711 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3209 3239 1178
Size: 30 165 30 1806 30 3880
Fragments arranged by size:
3880 1806 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2362 3062 1186
Size: 1176 700 4065
Fragments arranged by size:
4065 1176 700
BseYI C'CCAG_C
Cuts at: 5144 5640 5144
Size: 496 5445
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2237 2981 3163 1163
Size: 573 501 744 182 3941
Fragments arranged by size:
3941 744 573 501 182
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3391 4152 5180 1540
Size: 275 186 1390 761 1028 2301
Fragments arranged by size:
2301 1390 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2463 2922 2991 4002 4087 5158 2463
Size: 459 69 1011 85 1071 3246
Fragments arranged by size:
3246 1071 1011 459 85 69
BsmI GAATG_Cn'
Cuts at: 3452 3545 3452
Size: 93 5848
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2382 2463 2922 2991 3260 4002 4087
Size: 399 592 81 459 69 269 742 85
Cuts at: 4087 5158 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 592 459 399 269 85
81 69
BspEI T'CCGG_A
Cuts at: 5604 5604
Size: 5941
BspHI T'CATG_A
Cuts at: 3830 5560 3830
Size: 1730 4211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2163 2451 2892 3296 844
Size: 1319 288 441 404 3489
Fragments arranged by size:
3489 1319 441 404 288
BsrBI CCG'CTC
Cuts at: 1394 1823 3828 1394
Size: 429 2005 3507
Fragments arranged by size:
3507 2005 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2143 3060 4436 4610 65
Size: 1183 895 917 1376 174 1396
Fragments arranged by size:
1396 1376 1183 917 895 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4585 4772 1700
Size: 110 2775 187 2869
Fragments arranged by size:
2869 2775 187 110
BsrGI T'GTAC_A
Cuts at: 96 2889 96
Size: 2793 3148
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5941
BssSI C'ACGA_G
Cuts at: 2567 4001 5013 2567
Size: 1434 1012 3495
Fragments arranged by size:
3495 1434 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2967 2995 794
Size: 2173 28 3740
Fragments arranged by size:
3740 2173 28
BstBI TT'CG_AA
Cuts at: 4745 4745
Size: 5941
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5941
BstXI CCAn_nnnn'nTGG
Cuts at: 3043 3214 3043
Size: 171 5770
BstYI r'GATC_y
Cuts at: 1972 2269 3039 3271 3638 4023 4040 5492 5578
Size: 297 770 232 367 385 17 1452 86
Cuts at: 5578 5590 5936 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 770 385 367 346 297 232
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2853 2902 513
Size: 552 1788 49 3552
Fragments arranged by size:
3552 1788 552 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5202
Fragments arranged by size:
5202 683 56
BtsI GCAGTG_nn'
Cuts at: 2221 3457 4251 4271 2221
Size: 1236 794 20 3891
Fragments arranged by size:
3891 1236 794 20
ClaI AT'CG_AT
Cuts at: 1554 2190 1554
Size: 636 5305
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5906
DraI TTT'AAA
Cuts at: 3245 3245
Size: 5941
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4948 809
Size: 507 3632 1802
Fragments arranged by size:
3632 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2832 2956 3033
Size: 54 1425 50 275 18 1002 124 77
Cuts at: 3033 3388 4277 5906 8
Size: 355 889 1629 43
Fragments arranged by size:
1629 1425 1002 889 355 275 124 77
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3388 1537
Size: 275 1576 4090
Fragments arranged by size:
4090 1576 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2765 3871 1353
Size: 1412 1106 3423
Fragments arranged by size:
3423 1412 1106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2458 4528 4902 5048 387
Size: 1064 138 869 2070 374 146 1280
Fragments arranged by size:
2070 1280 1064 869 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2145 2252 3065 4004 4601 5388 5656
Size: 393 513 107 813 939 597 787 268
Cuts at: 5656 1239
Size: 1524
Fragments arranged by size:
1524 939 813 787 597 513 393 268
107
EcoNI CCTnn'n_nnAGG
Cuts at: 3047 3047
Size: 5941
EcoO109I rG'GnC_Cy
Cuts at: 2241 2323 3022 2241
Size: 82 699 5160
Fragments arranged by size:
5160 699 82
EcoRI G'AATT_C
Cuts at: 2131 3250 3628 2131
Size: 1119 378 4444
Fragments arranged by size:
4444 1119 378
EcoRV GAT'ATC
Cuts at: 2293 2293
Size: 5941
FspI TGC'GCA
Cuts at: 4447 4447
Size: 5941
HaeII r_GCGC'y
Cuts at: 1678 2098 2824 2853 2861 5088 1678
Size: 420 726 29 8 2227 2531
Fragments arranged by size:
2531 2227 726 420 29 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3187 3209 3219 3241 4582
Size: 32 135 32 1615 22 10 22 1341
Cuts at: 4582 4614 4656 4688 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1615 1341 135 42 32 32 32
32 22 22 10
HincII GTy'rAC
Cuts at: 669 1657 1938 3285 3531 669
Size: 988 281 1347 246 3079
Fragments arranged by size:
3079 1347 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5941
HpaI GTT'AAC
Cuts at: 3531 3531
Size: 5941
KasI G'GCGC_C
Cuts at: 2857 2857
Size: 5941
KpnI G_GTAC'C
Cuts at: 2606 3266 2606
Size: 660 5281
MfeI C'AATT_G
Cuts at: 2042 3540 2042
Size: 1498 4443
MluI A'CGCG_T
Cuts at: 5817 5817
Size: 5941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5055 5239 1305
Size: 3750 184 2007
Fragments arranged by size:
3750 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2834 3035 10
Size: 54 1425 343 1002 201 2916
Fragments arranged by size:
2916 1425 1002 343 201 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2120 3194 3899 4258 4417 518
Size: 648 954 1074 705 359 159 2042
Fragments arranged by size:
2042 1074 954 705 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2818 2904 3078 3357 4034 5182 5427 1606
Size: 1212 86 174 279 677 1148 245 2120
Fragments arranged by size:
2120 1212 1148 677 279 245 174 86
NarI GG'CG_CC
Cuts at: 2858 2858
Size: 5941
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5389
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5941
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5941
NotI GC'GGCC_GC
Cuts at: 3388 3388
Size: 5941
NspI r_CATG'y
Cuts at: 1132 1229 3299 4844 1132
Size: 97 2070 1545 2229
Fragments arranged by size:
2229 2070 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2524 1348
Size: 1176 4765
PciI A'CATG_T
Cuts at: 4840 4840
Size: 5941
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5941
PfoI T'CCnGG_A
Cuts at: 3042 3042
Size: 5941
PmeI GTTT'AAAC
Cuts at: 3245 3245
Size: 5941
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3101 3133 4010 4042 5523 5555 3101
Size: 32 877 32 1481 32 3487
Fragments arranged by size:
3487 1481 877 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2241 2323 3022 2241
Size: 82 699 5160
Fragments arranged by size:
5160 699 82
PshAI GACnn'nnGTC
Cuts at: 1613 2729 1613
Size: 1116 4825
PsiI TTA'TAA
Cuts at: 3511 3511
Size: 5941
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2873 2905 2873
Size: 32 5909
PstI C_TGCA'G
Cuts at: 830 2277 2503 2682 2828 3107 3293 830
Size: 1447 226 179 146 279 186 3478
Fragments arranged by size:
3478 1447 279 226 186 179 146
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5941
SacII CC_GC'GG
Cuts at: 2905 2905
Size: 5941
SalI G'TCGA_C
Cuts at: 1936 3283 1936
Size: 1347 4594
SanDI GG'GwC_CC
Cuts at: 3022 3022
Size: 5941
SbfI CC_TGCA'GG
Cuts at: 3293 3293
Size: 5941
ScaI AGT'ACT
Cuts at: 2621 4189 5901 2621
Size: 1568 1712 2661
Fragments arranged by size:
2661 1712 1568
SfcI C'TryA_G
Cuts at: 826 1045 2273 2499 2678 2824 3103 3289 4424
Size: 219 1228 226 179 146 279 186 1135
Cuts at: 4424 5105 5296 826
Size: 681 191 1471
Fragments arranged by size:
1471 1228 1135 681 279 226 219 191
186 179 146
SfoI GGC'GCC
Cuts at: 2859 2859
Size: 5941
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5941
SmaI CCC'GGG
Cuts at: 1872 2779 3268 1872
Size: 907 489 4545
Fragments arranged by size:
4545 907 489
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4045 4946 5208 820
Size: 197 926 2102 901 262 1553
Fragments arranged by size:
2102 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3732 493
Size: 3239 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5941
SphI G_CATG'C
Cuts at: 1132 3299 1132
Size: 2167 3774
SspI AAT'ATT
Cuts at: 5 52 2015 3865 5
Size: 47 1963 1850 2081
Fragments arranged by size:
2081 1963 1850 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5941
StyI C'CwwG_G
Cuts at: 513 1065 2265 3380 513
Size: 552 1200 1115 3074
Fragments arranged by size:
3074 1200 1115 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3151 3968 3985 4138 4323 4768 325
Size: 1255 1571 817 17 153 185 445 1498
Fragments arranged by size:
1571 1498 1255 817 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2619 2889 4187
Size: 274 80 33 51 1443 642 270 1298
Cuts at: 4187 5899 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1298 642 274 270 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3128 3160 5901 5933 3128
Size: 32 2741 32 3136
Fragments arranged by size:
3136 2741 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5941
XbaI T'CTAG_A
Cuts at: 3277 3277
Size: 5941
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2641 2641
Size: 5941
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5941
XmaI C'CCGG_G
Cuts at: 1870 2777 3266 1870
Size: 907 489 4545
Fragments arranged by size:
4545 907 489
XmnI GAAnn'nnTTC
Cuts at: 4070 4070
Size: 5941
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3749 276
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI SacII SalI
SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PvuII RsrII SacI SapI SexAI
SfiI SrfI SwaI