Restriction map for
pFN21AE5561
(Circular) (Six-base) MAPSORT of: pFN21AE5561.seq Check: 9231 from: 1 to: 5971
pFN21AE5561 5971 bp
With 182 enzymes: SgfI *
January 30, 2014 17:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3781 278
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3314 1937
Size: 1377 4594
Acc65I G'GTAC_C
Cuts at: 3292 3292
Size: 5971
AclI AA'CG_TT
Cuts at: 1563 2713 4098 4471 1563
Size: 1150 1385 373 3063
Fragments arranged by size:
3063 1385 1150 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2084 2380 3152 4034 5418 5686 1632
Size: 452 296 772 882 1384 268 1917
Fragments arranged by size:
1917 1384 882 772 452 296 268
AflII C'TTAA_G
Cuts at: 820 1017 2231 820
Size: 197 1214 4560
Fragments arranged by size:
4560 1214 197
AflIII A'CryG_T
Cuts at: 1203 4870 5847 1203
Size: 3667 977 1327
Fragments arranged by size:
3667 1327 977
AgeI A'CCGG_T
Cuts at: 4802 4802
Size: 5971
AhdI GACnn_n'nnGTC
Cuts at: 4700 4700
Size: 5971
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5971
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2555 1184
Size: 1371 4600
AlwNI CAG_nnn'CTG
Cuts at: 3714 5286 3714
Size: 1572 4399
ApaLI G'TGCA_C
Cuts at: 4028 5184 4028
Size: 1156 4815
ApoI r'AATT_y
Cuts at: 1493 2057 2805 3280 3506 3658 5767 1493
Size: 564 748 475 226 152 2109 1697
Fragments arranged by size:
2109 1697 748 564 475 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4525 160
Size: 872 3493 1606
Fragments arranged by size:
3493 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
AvaI C'yCGr_G
Cuts at: 1870 1943 3296 1870
Size: 73 1353 4545
Fragments arranged by size:
4545 1353 73
BamHI G'GATC_C
Cuts at: 3301 3668 3301
Size: 367 5604
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3292 4747 618
Size: 325 217 627 1505 1455 1842
Fragments arranged by size:
1842 1505 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2457 3075 3290 1391
Size: 1066 618 215 4072
Fragments arranged by size:
4072 1066 618 215
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2770 2799 3108 3158 928
Size: 971 871 29 309 50 3741
Fragments arranged by size:
3741 971 871 309 50 29
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4162 4196 1903
Size: 34 50 34 2141 34 3678
Fragments arranged by size:
3678 2141 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2539 3863 5073 2539
Size: 1324 1210 3437
Fragments arranged by size:
3437 1324 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5971
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4582 136
Size: 107 122 71 631 605 2910 1525
Fragments arranged by size:
2910 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5966 5966
Size: 5971
BlpI GC'TnA_GC
Cuts at: 2373 3388 2373
Size: 1015 4956
Bme1580I G_kGCm'C
Cuts at: 1790 2028 4032 5188 1790
Size: 238 2004 1156 2573
Fragments arranged by size:
2573 2004 1156 238
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5971
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3702 4660 442
Size: 416 53 83 399 24 2285 958 1753
Fragments arranged by size:
2285 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5971
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2036 2376 2468 4631 1239
Size: 797 340 92 2163 2579
Fragments arranged by size:
2579 2163 797 340 92
Bpu10I CC'TnA_GC
Cuts at: 2082 2082
Size: 5971
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4096 4961 5259 4096
Size: 865 298 4808
Fragments arranged by size:
4808 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4634 882
Size: 617 3135 2219
Fragments arranged by size:
3135 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3762 493
Size: 711 2558 2702
Fragments arranged by size:
2702 2558 711
BsaBI GATnn'nnATC
Cuts at: 5965 5965
Size: 5971
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3778 4160 4795 275
Size: 53 83 186 936 2245 382 635 1451
Fragments arranged by size:
2245 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4404 4802 5076 5223 5634 4404
Size: 398 274 147 411 4741
Fragments arranged by size:
4741 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2425 2455 2490 2520 2539
Size: 30 165 30 1022 30 35 30 19
Cuts at: 2539 2569 1178
Size: 30 4580
Fragments arranged by size:
4580 1022 165 35 30 30 30 30
30 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2145 1186
Size: 959 5012
BseYI C'CCAG_C
Cuts at: 2065 5174 5670 2065
Size: 3109 496 2366
Fragments arranged by size:
3109 2366 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3092 1163
Size: 573 1356 4042
Fragments arranged by size:
4042 1356 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3421 4182 5210 1540
Size: 275 186 1420 761 1028 2301
Fragments arranged by size:
2301 1420 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3075 4032 4117 5188 3075
Size: 957 85 1071 3858
Fragments arranged by size:
3858 1071 957 85
BsmI GAATG_Cn'
Cuts at: 2076 3482 3575 2076
Size: 1406 93 4472
Fragments arranged by size:
4472 1406 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2457 3075 3290 4032 4117 5188
Size: 399 238 429 618 215 742 85 1071
Cuts at: 5188 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 618 429 399 238 215
85
BspEI T'CCGG_A
Cuts at: 5634 5634
Size: 5971
BspHI T'CATG_A
Cuts at: 3860 5590 3860
Size: 1730 4241
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2627 2641 2654 3326 844
Size: 1783 14 13 672 3489
Fragments arranged by size:
3489 1783 672 14 13
BsrBI CCG'CTC
Cuts at: 1394 1823 3858 1394
Size: 429 2035 3507
Fragments arranged by size:
3507 2035 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2057 2593 3204 4466 4640 65
Size: 1183 809 536 611 1262 174 1396
Fragments arranged by size:
1396 1262 1183 809 611 536 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4615 4802 1700
Size: 110 2805 187 2869
Fragments arranged by size:
2869 2805 187 110
BsrGI T'GTAC_A
Cuts at: 96 2361 2517 96
Size: 2265 156 3550
Fragments arranged by size:
3550 2265 156
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5971
BssSI C'ACGA_G
Cuts at: 4031 5043 4031
Size: 1012 4959
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5971
BstBI TT'CG_AA
Cuts at: 4775 4775
Size: 5971
BstEII G'GTnAC_C
Cuts at: 1184 3214 1184
Size: 2030 3941
BstXI CCAn_nnnn'nTGG
Cuts at: 2339 2339
Size: 5971
BstYI r'GATC_y
Cuts at: 1972 2988 3301 3668 4053 4070 5522 5608 5620
Size: 1016 313 367 385 17 1452 86 12
Cuts at: 5620 5966 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1016 385 367 346 313 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2467 2494 513
Size: 552 1402 27 3990
Fragments arranged by size:
3990 1402 552 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2957 487
Size: 683 56 1731 3501
Fragments arranged by size:
3501 1731 683 56
BtsI GCAGTG_nn'
Cuts at: 3487 4281 4301 3487
Size: 794 20 5157
Fragments arranged by size:
5157 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5971
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5936
DraI TTT'AAA
Cuts at: 3275 3275
Size: 5971
DraIII CAC_nnn'GTG
Cuts at: 2914 3088 2914
Size: 174 5797
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4978 809
Size: 507 3662 1802
Fragments arranged by size:
3662 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3418 4307 5936
Size: 54 1425 50 275 18 1588 889 1629
Cuts at: 5936 8
Size: 43
Fragments arranged by size:
1629 1588 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3418 1537
Size: 275 1606 4090
Fragments arranged by size:
4090 1606 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2387 3901 1353
Size: 1034 1514 3423
Fragments arranged by size:
3423 1514 1034
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4558 4932 5078 387
Size: 1064 138 2969 374 146 1280
Fragments arranged by size:
2969 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3073 3073
Size: 5971
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2084 2376 2380 2468 3152 4034
Size: 393 404 48 292 4 88 684 882
Cuts at: 4034 4631 5418 5686 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 882 787 684 597 404 393 292
268 88 48 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2186 2186
Size: 5971
EcoRI G'AATT_C
Cuts at: 2057 3280 3658 2057
Size: 1223 378 4370
Fragments arranged by size:
4370 1223 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2765 2765
Size: 5971
FspI TGC'GCA
Cuts at: 4477 4477
Size: 5971
HaeII r_GCGC'y
Cuts at: 1678 5118 1678
Size: 3440 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2425 2457 2871 2903 4612
Size: 32 135 32 853 32 414 32 1709
Cuts at: 4612 4644 4686 4718 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1709 853 414 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3315 3561 669
Size: 988 281 1377 246 3079
Fragments arranged by size:
3079 1377 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5971
HpaI GTT'AAC
Cuts at: 3561 3561
Size: 5971
KpnI G_GTAC'C
Cuts at: 3296 3296
Size: 5971
MfeI C'AATT_G
Cuts at: 3570 3570
Size: 5971
MluI A'CGCG_T
Cuts at: 5847 5847
Size: 5971
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5085 5269 1305
Size: 3780 184 2007
Fragments arranged by size:
3780 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4149
Fragments arranged by size:
4149 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2842 2976 3929 4288 4447 518
Size: 648 1676 134 953 359 159 2042
Fragments arranged by size:
2042 1676 953 648 359 159 134
MspA1I CmG'CkG
Cuts at: 1606 2372 3260 3387 4064 5212 5457 1606
Size: 766 888 127 677 1148 245 2120
Fragments arranged by size:
2120 1148 888 766 677 245 127
NcoI C'CATG_G
Cuts at: 513 1065 2467 2494 513
Size: 552 1402 27 3990
Fragments arranged by size:
3990 1402 552 27
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5971
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5971
NotI GC'GGCC_GC
Cuts at: 3418 3418
Size: 5971
NspI r_CATG'y
Cuts at: 1132 1229 2758 3039 3329 4874 1132
Size: 97 1529 281 290 1545 2229
Fragments arranged by size:
2229 1545 1529 290 281 97
PasI CC'CwG_GG
Cuts at: 1348 2945 1348
Size: 1597 4374
PciI A'CATG_T
Cuts at: 4870 4870
Size: 5971
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2473 3088 1108
Size: 1365 615 3991
Fragments arranged by size:
3991 1365 615
PfoI T'CCnGG_A
Cuts at: 2353 3066 2353
Size: 713 5258
PmeI GTTT'AAAC
Cuts at: 3275 3275
Size: 5971
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4040 4072 5553 5585 4040
Size: 32 1481 32 4426
Fragments arranged by size:
4426 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5971
PsiI TTA'TAA
Cuts at: 2955 3541 2955
Size: 586 5385
PstI C_TGCA'G
Cuts at: 830 2544 2638 2665 3323 830
Size: 1714 94 27 658 3478
Fragments arranged by size:
3478 1714 658 94 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5971
SacI G_AGCT'C
Cuts at: 3075 3075
Size: 5971
SalI G'TCGA_C
Cuts at: 1936 3313 1936
Size: 1377 4594
SapI GCTCTTCn'nnn_
Cuts at: 2387 2387
Size: 5971
SbfI CC_TGCA'GG
Cuts at: 2544 2638 3323 2544
Size: 94 685 5192
Fragments arranged by size:
5192 685 94
ScaI AGT'ACT
Cuts at: 4219 5931 4219
Size: 1712 4259
SfcI C'TryA_G
Cuts at: 826 1045 2140 2524 2540 2634 2661 3046 3319
Size: 219 1095 384 16 94 27 385 273
Cuts at: 3319 4454 5135 5326 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1095 681 385 384 273 219
191 94 27 16
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5971
SmaI CCC'GGG
Cuts at: 1872 3298 1872
Size: 1426 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2231 4075 4976 5238 820
Size: 197 926 288 1844 901 262 1553
Fragments arranged by size:
1844 1553 926 901 288 262 197
SnaBI TAC'GTA
Cuts at: 493 3762 493
Size: 3269 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5971
SphI G_CATG'C
Cuts at: 1132 2758 3329 1132
Size: 1626 571 3774
Fragments arranged by size:
3774 1626 571
SspI AAT'ATT
Cuts at: 5 52 2276 3895 5
Size: 47 2224 1619 2081
Fragments arranged by size:
2224 2081 1619 47
StuI AGG'CCT
Cuts at: 1517 3238 1517
Size: 1721 4250
StyI C'CwwG_G
Cuts at: 513 1065 2467 2494 2550 3410 513
Size: 552 1402 27 56 860 3074
Fragments arranged by size:
3074 1402 860 552 56 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3998 4015 4168 4353 4798 325
Size: 1255 2418 17 153 185 445 1498
Fragments arranged by size:
2418 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2361 2517 4217
Size: 274 80 33 51 1443 384 156 1700
Cuts at: 4217 5929 96
Size: 1712 138
Fragments arranged by size:
1712 1700 1443 384 274 156 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3101 3133 5931 5963 3101
Size: 32 2798 32 3109
Fragments arranged by size:
3109 2798 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5971
XbaI T'CTAG_A
Cuts at: 3307 3307
Size: 5971
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2492 2011
Size: 481 5490
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5971
XmaI C'CCGG_G
Cuts at: 1870 3296 1870
Size: 1426 4545
XmnI GAAnn'nnTTC
Cuts at: 4100 4100
Size: 5971
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3779 276
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI