Restriction map for
pFN21AE5560
(Circular) (Six-base) MAPSORT of: pFN21AE5560.seq Check: 3689 from: 1 to: 5839
pFN21AE5560 5839 bp
With 182 enzymes: SgfI *
January 30, 2014 17:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3649 278
Size: 53 83 186 936 2113 2468
Fragments arranged by size:
2468 2113 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2161 3182 1937
Size: 224 1021 4594
Fragments arranged by size:
4594 1021 224
Acc65I G'GTAC_C
Cuts at: 3160 3160
Size: 5839
AclI AA'CG_TT
Cuts at: 1563 3966 4339 1563
Size: 2403 373 3063
Fragments arranged by size:
3063 2403 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2535 2970 3902 5286 5554 1632
Size: 903 435 932 1384 268 1917
Fragments arranged by size:
1917 1384 932 903 435 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5642
AflIII A'CryG_T
Cuts at: 1203 4738 5715 1203
Size: 3535 977 1327
Fragments arranged by size:
3535 1327 977
AgeI A'CCGG_T
Cuts at: 4670 4670
Size: 5839
AhdI GACnn_n'nnGTC
Cuts at: 2434 4568 2434
Size: 2134 3705
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5839
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5839
AlwNI CAG_nnn'CTG
Cuts at: 2570 2627 2854 2875 2948 2992 3582 5154 2570
Size: 57 227 21 73 44 590 1572 3255
Fragments arranged by size:
3255 1572 590 227 73 57 44 21
ApaLI G'TGCA_C
Cuts at: 3896 5052 3896
Size: 1156 4683
ApoI r'AATT_y
Cuts at: 1493 2573 3014 3148 3374 3526 5635 1493
Size: 1080 441 134 226 152 2109 1697
Fragments arranged by size:
2109 1697 1080 441 226 152 134
AseI AT'TA_AT
Cuts at: 160 1032 4393 160
Size: 872 3361 1606
Fragments arranged by size:
3361 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5839
AvaI C'yCGr_G
Cuts at: 1870 1943 3164 1870
Size: 73 1221 4545
Fragments arranged by size:
4545 1221 73
BamHI G'GATC_C
Cuts at: 3169 3536 3169
Size: 367 5472
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3160 4615 618
Size: 325 217 627 1373 1455 1842
Fragments arranged by size:
1842 1455 1373 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2084 2257 3158 1391
Size: 693 173 901 4072
Fragments arranged by size:
4072 901 693 173
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2424 928
Size: 971 525 4343
Fragments arranged by size:
4343 971 525
BbvCI CC'TCA_GC
Cuts at: 2015 2653 2015
Size: 638 5201
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4030 4064 1903
Size: 34 50 34 2009 34 3678
Fragments arranged by size:
3678 2009 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3731 4941 3731
Size: 1210 4629
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5839
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4450 136
Size: 107 122 71 631 605 2778 1525
Fragments arranged by size:
2778 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5834 5834
Size: 5839
BlpI GC'TnA_GC
Cuts at: 3256 3256
Size: 5839
Bme1580I G_kGCm'C
Cuts at: 1790 3900 5056 1790
Size: 2110 1156 2573
Fragments arranged by size:
2573 2110 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5839
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3570 4528 442
Size: 416 53 83 399 24 2153 958 1753
Fragments arranged by size:
2153 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5839
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1994 2326 2520 2825 4499 1239
Size: 755 332 194 305 1674 2579
Fragments arranged by size:
2579 1674 755 332 305 194
Bpu10I CC'TnA_GC
Cuts at: 2015 2653 2015
Size: 638 5201
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3964 4829 5127 3964
Size: 865 298 4676
Fragments arranged by size:
4676 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4502 882
Size: 617 3003 2219
Fragments arranged by size:
3003 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3630 493
Size: 711 2426 2702
Fragments arranged by size:
2702 2426 711
BsaBI GATnn'nnATC
Cuts at: 5833 5833
Size: 5839
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3646 4028 4663 275
Size: 53 83 186 936 2113 382 635 1451
Fragments arranged by size:
2113 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2440 4272 4670 4944 5091 5502 2440
Size: 1832 398 274 147 411 2777
Fragments arranged by size:
2777 1832 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2518 2548 1178
Size: 30 165 30 1115 30 4469
Fragments arranged by size:
4469 1115 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2577 1186
Size: 1391 4448
BseYI C'CCAG_C
Cuts at: 2083 2668 5042 5538 2083
Size: 585 2374 496 2384
Fragments arranged by size:
2384 2374 585 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2904 1163
Size: 573 1168 4098
Fragments arranged by size:
4098 1168 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3289 4050 5078 1540
Size: 275 186 1288 761 1028 2301
Fragments arranged by size:
2301 1288 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2257 3900 3985 5056 2257
Size: 1643 85 1071 3040
Fragments arranged by size:
3040 1643 1071 85
BsmI GAATG_Cn'
Cuts at: 2090 2976 3350 3443 2090
Size: 886 374 93 4486
Fragments arranged by size:
4486 886 374 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2084 2257 3158 3900 3985 5056 1391
Size: 399 294 173 901 742 85 1071 2174
Fragments arranged by size:
2174 1071 901 742 399 294 173 85
BspEI T'CCGG_A
Cuts at: 2440 5502 2440
Size: 3062 2777
BspHI T'CATG_A
Cuts at: 3728 5458 3728
Size: 1730 4109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3194 844
Size: 2350 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3726 1394
Size: 429 1903 3507
Fragments arranged by size:
3507 1903 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4334 4508 65
Size: 1183 3086 174 1396
Fragments arranged by size:
3086 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4483 4670 1700
Size: 110 2673 187 2869
Fragments arranged by size:
2869 2673 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5839
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5839
BssSI C'ACGA_G
Cuts at: 3899 4911 3899
Size: 1012 4827
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2727 794
Size: 1933 3906
BstBI TT'CG_AA
Cuts at: 4643 4643
Size: 5839
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5839
BstYI r'GATC_y
Cuts at: 1972 2734 2871 3169 3536 3921 3938 5390 5476
Size: 762 137 298 367 385 17 1452 86
Cuts at: 5476 5488 5834 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 762 385 367 346 298 137
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2612 2744 513
Size: 552 1547 132 3608
Fragments arranged by size:
3608 1547 552 132
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5100
Fragments arranged by size:
5100 683 56
BtsI GCAGTG_nn'
Cuts at: 2431 3355 4149 4169 2431
Size: 924 794 20 4101
Fragments arranged by size:
4101 924 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5839
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5804
DraI TTT'AAA
Cuts at: 3143 3143
Size: 5839
DraIII CAC_nnn'GTG
Cuts at: 2944 2962 2944
Size: 18 5821
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4846 809
Size: 507 3530 1802
Fragments arranged by size:
3530 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3286 4175 5804
Size: 54 1425 50 275 18 1456 889 1629
Cuts at: 5804 8
Size: 43
Fragments arranged by size:
1629 1456 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3286 1537
Size: 275 1474 4090
Fragments arranged by size:
4090 1474 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2072 3769 1353
Size: 719 1697 3423
Fragments arranged by size:
3423 1697 719
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4426 4800 4946 387
Size: 1064 138 2837 374 146 1280
Fragments arranged by size:
2837 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2255 2255
Size: 5839
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1994 2326 2520 2535 2825 2970 3902
Size: 393 362 332 194 15 290 145 932
Cuts at: 3902 4499 5286 5554 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 932 787 597 393 362 332 290
268 194 145 15
EcoRI G'AATT_C
Cuts at: 2573 3148 3526 2573
Size: 575 378 4886
Fragments arranged by size:
4886 575 378
FspI TGC'GCA
Cuts at: 4345 4345
Size: 5839
HaeII r_GCGC'y
Cuts at: 1678 4986 1678
Size: 3308 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2143 2175 2420 2452 2863
Size: 32 135 32 571 32 245 32 411
Cuts at: 2863 2895 4480 4512 4554 4586 1373
Size: 32 1585 32 42 32 2626
Fragments arranged by size:
2626 1585 571 411 245 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2162 3183 3429 669
Size: 988 281 224 1021 246 3079
Fragments arranged by size:
3079 1021 988 281 246 224
HindIII A'AGCT_T
Cuts at: 748 2110 2931 748
Size: 1362 821 3656
Fragments arranged by size:
3656 1362 821
HpaI GTT'AAC
Cuts at: 3429 3429
Size: 5839
KpnI G_GTAC'C
Cuts at: 3164 3164
Size: 5839
MfeI C'AATT_G
Cuts at: 3438 3438
Size: 5839
MluI A'CGCG_T
Cuts at: 5715 5715
Size: 5839
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2615 4953 5137 1305
Size: 1310 2338 184 2007
Fragments arranged by size:
2338 2007 1310 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4017
Fragments arranged by size:
4017 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3797 4156 4315 518
Size: 648 2631 359 159 2042
Fragments arranged by size:
2631 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2014 2087 2854 3255 3932 5080 5325 1606
Size: 408 73 767 401 677 1148 245 2120
Fragments arranged by size:
2120 1148 767 677 408 401 245 73
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5287
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5839
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5839
NotI GC'GGCC_GC
Cuts at: 3286 3286
Size: 5839
NspI r_CATG'y
Cuts at: 1132 1229 3197 4742 1132
Size: 97 1968 1545 2229
Fragments arranged by size:
2229 1968 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5839
PciI A'CATG_T
Cuts at: 4738 4738
Size: 5839
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5839
PmeI GTTT'AAAC
Cuts at: 3143 3143
Size: 5839
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3908 3940 5421 5453 3908
Size: 32 1481 32 4294
Fragments arranged by size:
4294 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5839
PsiI TTA'TAA
Cuts at: 3409 3409
Size: 5839
PstI C_TGCA'G
Cuts at: 830 2229 2599 2623 2871 3191 830
Size: 1399 370 24 248 320 3478
Fragments arranged by size:
3478 1399 370 320 248 24
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5839
PvuII CAG'CTG
Cuts at: 2014 2854 2014
Size: 840 4999
SacI G_AGCT'C
Cuts at: 2257 2257
Size: 5839
SalI G'TCGA_C
Cuts at: 1936 2160 3181 1936
Size: 224 1021 4594
Fragments arranged by size:
4594 1021 224
SapI GCTCTTCn'nnn_
Cuts at: 2072 2072
Size: 5839
SbfI CC_TGCA'GG
Cuts at: 2229 3191 2229
Size: 962 4877
ScaI AGT'ACT
Cuts at: 3088 4087 5799 3088
Size: 999 1712 3128
Fragments arranged by size:
3128 1712 999
SexAI A'CCwGG_T
Cuts at: 2296 2296
Size: 5839
SfcI C'TryA_G
Cuts at: 826 1045 2225 2595 2619 2867 3187 4322 5003
Size: 219 1180 370 24 248 320 1135 681
Cuts at: 5003 5194 826
Size: 191 1471
Fragments arranged by size:
1471 1180 1135 681 370 320 248 219
191 24
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5839
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5839
SmaI CCC'GGG
Cuts at: 1872 3166 1872
Size: 1294 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3943 4844 5106 820
Size: 197 926 2000 901 262 1553
Fragments arranged by size:
2000 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3630 493
Size: 3137 2702
SpeI A'CTAG_T
Cuts at: 152 2047 152
Size: 1895 3944
SphI G_CATG'C
Cuts at: 1132 3197 1132
Size: 2065 3774
SspI AAT'ATT
Cuts at: 5 52 3763 5
Size: 47 3711 2081
Fragments arranged by size:
3711 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5839
StyI C'CwwG_G
Cuts at: 513 1065 3278 513
Size: 552 2213 3074
Fragments arranged by size:
3074 2213 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3866 3883 4036 4221 4666 325
Size: 1255 2286 17 153 185 445 1498
Fragments arranged by size:
2286 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3086 4085 5797
Size: 274 80 33 51 1443 1109 999 1712
Cuts at: 5797 96
Size: 138
Fragments arranged by size:
1712 1443 1109 999 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5799 5831 5799
Size: 32 5807
Tth111I GACn'n_nGTC
Cuts at: 1316 2298 1316
Size: 982 4857
XbaI T'CTAG_A
Cuts at: 3175 3175
Size: 5839
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5839
XmaI C'CCGG_G
Cuts at: 1870 3164 1870
Size: 1294 4545
XmnI GAAnn'nnTTC
Cuts at: 3968 3968
Size: 5839
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3647 276
Size: 53 83 186 936 2113 2468
Fragments arranged by size:
2468 2113 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI
SwaI XcmI