Restriction map for
pFN21AE5559
(Circular) (Six-base) MAPSORT of: pFN21AE5559.seq Check: 2982 from: 1 to: 5917
pFN21AE5559 5917 bp
With 182 enzymes: SgfI *
January 30, 2014 17:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1999 1999
Size: 5917
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3727 278
Size: 53 83 186 936 2191 2468
Fragments arranged by size:
2468 2191 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3260 1937
Size: 1323 4594
Acc65I G'GTAC_C
Cuts at: 2291 2856 3238 2291
Size: 565 382 4970
Fragments arranged by size:
4970 565 382
AclI AA'CG_TT
Cuts at: 1563 4044 4417 1563
Size: 2481 373 3063
Fragments arranged by size:
3063 2481 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3980 5364 5632 1632
Size: 2348 1384 268 1917
Fragments arranged by size:
2348 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5720
AflIII A'CryG_T
Cuts at: 1203 4816 5793 1203
Size: 3613 977 1327
Fragments arranged by size:
3613 1327 977
AgeI A'CCGG_T
Cuts at: 4748 4748
Size: 5917
AhdI GACnn_n'nnGTC
Cuts at: 4646 4646
Size: 5917
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5917
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5917
AlwNI CAG_nnn'CTG
Cuts at: 2668 3145 3660 5232 2668
Size: 477 515 1572 3353
Fragments arranged by size:
3353 1572 515 477
ApaI G_GGCC'C
Cuts at: 2087 2206 2087
Size: 119 5798
ApaLI G'TGCA_C
Cuts at: 3974 5130 3974
Size: 1156 4761
ApoI r'AATT_y
Cuts at: 1493 3226 3452 3604 5713 1493
Size: 1733 226 152 2109 1697
Fragments arranged by size:
2109 1733 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4471 160
Size: 872 3439 1606
Fragments arranged by size:
3439 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5917
AvaI C'yCGr_G
Cuts at: 1870 1943 2087 2163 3242 1870
Size: 73 144 76 1079 4545
Fragments arranged by size:
4545 1079 144 76 73
AvrII C'CTAG_G
Cuts at: 2251 2287 2422 2251
Size: 36 135 5746
Fragments arranged by size:
5746 135 36
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2283 2316 2283
Size: 33 5884
BamHI G'GATC_C
Cuts at: 2552 3247 3614 2552
Size: 695 367 4855
Fragments arranged by size:
4855 695 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2291 2856 3238 4693 618
Size: 325 217 627 504 565 382 1455 1842
Fragments arranged by size:
1842 1455 627 565 504 382 325 217
BanII G_rGCy'C
Cuts at: 1391 2087 2206 2823 3189 3236 1391
Size: 696 119 617 366 47 4072
Fragments arranged by size:
4072 696 617 366 119 47
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2258 928
Size: 971 359 4587
Fragments arranged by size:
4587 971 359
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2055 4108 4142 1903
Size: 34 50 34 34 2053 34 3678
Fragments arranged by size:
3678 2053 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2284 2570 3809 5019 2284
Size: 286 1239 1210 3182
Fragments arranged by size:
3182 1239 1210 286
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5917
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2091 4528 136
Size: 107 122 71 631 605 419 2437 1525
Fragments arranged by size:
2437 1525 631 605 419 122 107 71
BglII A'GATC_T
Cuts at: 2316 5912 2316
Size: 3596 2321
BlpI GC'TnA_GC
Cuts at: 3146 3334 3146
Size: 188 5729
Bme1580I G_kGCm'C
Cuts at: 1790 2087 2124 2206 3978 5134 1790
Size: 297 37 82 1772 1156 2573
Fragments arranged by size:
2573 1772 1156 297 82 37
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5917
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2689 3648 4606
Size: 416 53 83 399 24 1272 959 958
Cuts at: 4606 442
Size: 1753
Fragments arranged by size:
1753 1272 959 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5917
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2740 2740
Size: 5917
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2552 2739 4577 1239
Size: 1313 187 1838 2579
Fragments arranged by size:
2579 1838 1313 187
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2420 2930 4042 4907 5205 2420
Size: 510 1112 865 298 3132
Fragments arranged by size:
3132 1112 865 510 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2879 4580 882
Size: 617 1380 1701 2219
Fragments arranged by size:
2219 1701 1380 617
BsaAI yAC'GTr
Cuts at: 493 1204 2581 3708 493
Size: 711 1377 1127 2702
Fragments arranged by size:
2702 1377 1127 711
BsaBI GATnn'nnATC
Cuts at: 5911 5911
Size: 5917
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3724 4106 4741 275
Size: 53 83 186 936 2191 382 635 1451
Fragments arranged by size:
2191 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4350 4748 5022 5169 5580 4350
Size: 398 274 147 411 4687
Fragments arranged by size:
4687 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5692
Fragments arranged by size:
5692 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2467 2642 1186
Size: 1281 175 4461
Fragments arranged by size:
4461 1281 175
BseYI C'CCAG_C
Cuts at: 2188 2198 2572 3022 3039 3091 5120 5616 2188
Size: 10 374 450 17 52 2029 496 2489
Fragments arranged by size:
2489 2029 496 450 374 52 17 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2154 2978 3013 1163
Size: 573 418 824 35 4067
Fragments arranged by size:
4067 824 573 418 35
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2081 2112 2224 3367 4128 5156
Size: 275 186 80 31 112 1143 761 1028
Cuts at: 5156 1540
Size: 2301
Fragments arranged by size:
2301 1143 1028 761 275 186 112 80
31
BsiHKAI G_wGCw'C
Cuts at: 2219 2778 2823 3978 4063 5134 2219
Size: 559 45 1155 85 1071 3002
Fragments arranged by size:
3002 1155 1071 559 85 45
BsmI GAATG_Cn'
Cuts at: 3205 3428 3521 3205
Size: 223 93 5601
Fragments arranged by size:
5601 223 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 2124 2206 2219 2778 2823 3189
Size: 399 297 37 82 13 559 45 366
Cuts at: 3189 3236 3978 4063 5134 1391
Size: 47 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 559 399 366 297 85
82 47 45 37 13
BspEI T'CCGG_A
Cuts at: 5580 5580
Size: 5917
BspHI T'CATG_A
Cuts at: 3806 5536 3806
Size: 1730 4187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 1999 3272 844
Size: 1155 1273 3489
Fragments arranged by size:
3489 1273 1155
BsrBI CCG'CTC
Cuts at: 1394 1823 3804 1394
Size: 429 1981 3507
Fragments arranged by size:
3507 1981 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4412 4586 65
Size: 1183 3164 174 1396
Fragments arranged by size:
3164 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2107 2111 2219 4561 4748 1700
Size: 110 297 4 108 2342 187 2869
Fragments arranged by size:
2869 2342 297 187 110 108 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5917
BssHII G'CGCG_C
Cuts at: 1925 3132 1925
Size: 1207 4710
BssSI C'ACGA_G
Cuts at: 3977 4989 3977
Size: 1012 4905
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5917
BstBI TT'CG_AA
Cuts at: 4721 4721
Size: 5917
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5917
BstXI CCAn_nnnn'nTGG
Cuts at: 2834 2995 2834
Size: 161 5756
BstYI r'GATC_y
Cuts at: 1972 2316 2552 3247 3614 3999 4016 5468 5554
Size: 344 236 695 367 385 17 1452 86
Cuts at: 5554 5566 5912 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 695 385 367 346 344 236
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2727 3033 513
Size: 552 1662 306 3397
Fragments arranged by size:
3397 1662 552 306
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5178
Fragments arranged by size:
5178 683 56
BtsI GCAGTG_nn'
Cuts at: 2059 3073 3433 4227 4247 2059
Size: 1014 360 794 20 3729
Fragments arranged by size:
3729 1014 794 360 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5917
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5882
DraI TTT'AAA
Cuts at: 3221 3221
Size: 5917
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4924 809
Size: 507 3608 1802
Fragments arranged by size:
3608 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2078 2109 2221
Size: 54 1425 50 275 18 248 31 112
Cuts at: 2221 2730 3364 4253 5882 8
Size: 509 634 889 1629 43
Fragments arranged by size:
1629 1425 889 634 509 275 248 112
54 50 43 31 18
EagI C'GGCC_G
Cuts at: 1537 1812 2078 2109 2221 3364 1537
Size: 275 266 31 112 1143 4090
Fragments arranged by size:
4090 1143 275 266 112 31
EarI CTCTTCn'nnn_
Cuts at: 1353 3847 1353
Size: 2494 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4504 4878 5024 387
Size: 1064 138 2915 374 146 1280
Fragments arranged by size:
2915 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2821 2821
Size: 5917
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2552 2739 3980 4577 5364 5632 1239
Size: 393 920 187 1241 597 787 268 1524
Fragments arranged by size:
1524 1241 920 787 597 393 268 187
EcoO109I rG'GnC_Cy
Cuts at: 2084 2202 2203 2665 2919 2084
Size: 118 1 462 254 5082
Fragments arranged by size:
5082 462 254 118 1
EcoRI G'AATT_C
Cuts at: 3226 3604 3226
Size: 378 5539
FseI GG_CCGG'CC
Cuts at: 2111 2111
Size: 5917
FspI TGC'GCA
Cuts at: 4423 4423
Size: 5917
HaeII r_GCGC'y
Cuts at: 1678 2195 5064 1678
Size: 517 2869 2531
Fragments arranged by size:
2869 2531 517
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4558 4590 4632 4664 1373
Size: 32 135 32 2986 32 42 32 2626
Fragments arranged by size:
2986 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3261 3507 669
Size: 988 281 1323 246 3079
Fragments arranged by size:
3079 1323 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5917
HpaI GTT'AAC
Cuts at: 3507 3507
Size: 5917
KpnI G_GTAC'C
Cuts at: 2295 2860 3242 2295
Size: 565 382 4970
Fragments arranged by size:
4970 565 382
MfeI C'AATT_G
Cuts at: 3516 3516
Size: 5917
MluI A'CGCG_T
Cuts at: 5793 5793
Size: 5917
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2429 2821 5031 5215 1305
Size: 1124 392 2210 184 2007
Fragments arranged by size:
2210 2007 1124 392 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2732 10
Size: 54 1425 343 900 3195
Fragments arranged by size:
3195 1425 900 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2234 2692 3875 4234 4393 518
Size: 648 843 225 458 1183 359 159 2042
Fragments arranged by size:
2042 1183 843 648 458 359 225 159
MspA1I CmG'CkG
Cuts at: 1606 2198 2572 2812 3022 3154 3333 4010 5158
Size: 592 374 240 210 132 179 677 1148
Cuts at: 5158 5403 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 592 374 245 240 210
179 132
NaeI GCC'GGC
Cuts at: 2109 2113 2109
Size: 4 5913
NcoI C'CATG_G
Cuts at: 513 1065 2727 513
Size: 552 1662 3703
Fragments arranged by size:
3703 1662 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5917
NgoMIV G'CCGG_C
Cuts at: 2107 2111 2107
Size: 4 5913
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5917
NotI GC'GGCC_GC
Cuts at: 3364 3364
Size: 5917
NspI r_CATG'y
Cuts at: 1132 1229 3275 4820 1132
Size: 97 2046 1545 2229
Fragments arranged by size:
2229 2046 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5917
PciI A'CATG_T
Cuts at: 4816 4816
Size: 5917
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2668 1108
Size: 1560 4357
PmeI GTTT'AAAC
Cuts at: 3221 3221
Size: 5917
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3986 4018 5499 5531 3986
Size: 32 1481 32 4372
Fragments arranged by size:
4372 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2651 1613
Size: 1038 4879
PsiI TTA'TAA
Cuts at: 3487 3487
Size: 5917
PspOMI G'GGCC_C
Cuts at: 2083 2202 2083
Size: 119 5798
PstI C_TGCA'G
Cuts at: 830 2707 3269 830
Size: 1877 562 3478
Fragments arranged by size:
3478 1877 562
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5917
PvuII CAG'CTG
Cuts at: 2572 2812 3022 3154 2572
Size: 240 210 132 5335
Fragments arranged by size:
5335 240 210 132
SacI G_AGCT'C
Cuts at: 2823 2823
Size: 5917
SalI G'TCGA_C
Cuts at: 1936 3259 1936
Size: 1323 4594
SbfI CC_TGCA'GG
Cuts at: 3269 3269
Size: 5917
ScaI AGT'ACT
Cuts at: 4165 5877 4165
Size: 1712 4205
SfcI C'TryA_G
Cuts at: 826 1045 2349 2703 3137 3265 4400 5081 5272
Size: 219 1304 354 434 128 1135 681 191
Cuts at: 5272 826
Size: 1471
Fragments arranged by size:
1471 1304 1135 681 434 354 219 191
128
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5917
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5917
SmaI CCC'GGG
Cuts at: 1872 2089 3244 1872
Size: 217 1155 4545
Fragments arranged by size:
4545 1155 217
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2435 2945 4021 4922 5184 820
Size: 197 926 492 510 1076 901 262 1553
Fragments arranged by size:
1553 1076 926 901 510 492 262 197
SnaBI TAC'GTA
Cuts at: 493 3708 493
Size: 3215 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5917
SphI G_CATG'C
Cuts at: 1132 3275 1132
Size: 2143 3774
SspI AAT'ATT
Cuts at: 5 52 3841 5
Size: 47 3789 2081
Fragments arranged by size:
3789 2081 47
StuI AGG'CCT
Cuts at: 1517 2720 1517
Size: 1203 4714
StyI C'CwwG_G
Cuts at: 513 1065 2157 2251 2287 2422 2727 3356 513
Size: 552 1092 94 36 135 305 629 3074
Fragments arranged by size:
3074 1092 629 552 305 135 94 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3944 3961 4114 4299 4744 325
Size: 1255 2364 17 153 185 445 1498
Fragments arranged by size:
2364 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4163 5875 96
Size: 274 80 33 51 1443 2186 1712 138
Fragments arranged by size:
2186 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2051 2083 5877 5909 2051
Size: 32 3794 32 2059
Fragments arranged by size:
3794 2059 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2588 1316
Size: 1272 4645
XbaI T'CTAG_A
Cuts at: 3253 3253
Size: 5917
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2620 2620
Size: 5917
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5917
XmaI C'CCGG_G
Cuts at: 1870 2087 3242 1870
Size: 217 1155 4545
Fragments arranged by size:
4545 1155 217
XmnI GAAnn'nnTTC
Cuts at: 4046 4046
Size: 5917
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3725 276
Size: 53 83 186 936 2191 2468
Fragments arranged by size:
2468 2191 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI BbeI BbvCI BfrBI Bpu10I BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI