Restriction map for
pFN21AE5558
(Circular) (Six-base) MAPSORT of: pFN21AE5558.seq Check: 5740 from: 1 to: 5848
pFN21AE5558 5848 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3658 278
Size: 53 83 186 936 2122 2468
Fragments arranged by size:
2468 2122 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3191 1937
Size: 1254 4594
Acc65I G'GTAC_C
Cuts at: 3169 3169
Size: 5848
AclI AA'CG_TT
Cuts at: 1563 3975 4348 1563
Size: 2412 373 3063
Fragments arranged by size:
3063 2412 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2396 3114 3911 5295 5563 1632
Size: 764 718 797 1384 268 1917
Fragments arranged by size:
1917 1384 797 764 718 268
AflII C'TTAA_G
Cuts at: 820 1017 3107 820
Size: 197 2090 3561
Fragments arranged by size:
3561 2090 197
AflIII A'CryG_T
Cuts at: 1203 4747 5724 1203
Size: 3544 977 1327
Fragments arranged by size:
3544 1327 977
AgeI A'CCGG_T
Cuts at: 4679 4679
Size: 5848
AhdI GACnn_n'nnGTC
Cuts at: 4577 4577
Size: 5848
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5848
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5848
AlwNI CAG_nnn'CTG
Cuts at: 3591 5163 3591
Size: 1572 4276
ApaLI G'TGCA_C
Cuts at: 3905 5061 3905
Size: 1156 4692
ApoI r'AATT_y
Cuts at: 1493 2054 2406 3157 3383 3535 5644 1493
Size: 561 352 751 226 152 2109 1697
Fragments arranged by size:
2109 1697 751 561 352 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4402 160
Size: 872 3370 1606
Fragments arranged by size:
3370 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5848
AvaI C'yCGr_G
Cuts at: 1870 1943 3173 1870
Size: 73 1230 4545
Fragments arranged by size:
4545 1230 73
BamHI G'GATC_C
Cuts at: 3178 3545 3178
Size: 367 5481
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3169 4624 618
Size: 325 217 627 1382 1455 1842
Fragments arranged by size:
1842 1455 1382 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3085 3167 1391
Size: 1694 82 4072
Fragments arranged by size:
4072 1694 82
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2327 2491 928
Size: 971 428 164 4285
Fragments arranged by size:
4285 971 428 164
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4039 4073 1903
Size: 34 2102 34 3678
Fragments arranged by size:
3678 2102 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3740 4950 3740
Size: 1210 4638
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5848
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4459 136
Size: 107 122 71 631 605 2787 1525
Fragments arranged by size:
2787 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2356 5843 2356
Size: 3487 2361
BlpI GC'TnA_GC
Cuts at: 3265 3265
Size: 5848
Bme1580I G_kGCm'C
Cuts at: 1790 3909 5065 1790
Size: 2119 1156 2573
Fragments arranged by size:
2573 2119 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5848
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3086 3579 4537
Size: 416 53 83 399 24 1669 493 958
Cuts at: 4537 442
Size: 1753
Fragments arranged by size:
1753 1669 958 493 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5848
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2042 4508 1239
Size: 803 2466 2579
Fragments arranged by size:
2579 2466 803
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3973 4838 5136 3973
Size: 865 298 4685
Fragments arranged by size:
4685 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4511 882
Size: 617 3012 2219
Fragments arranged by size:
3012 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3639 493
Size: 711 2435 2702
Fragments arranged by size:
2702 2435 711
BsaBI GATnn'nnATC
Cuts at: 5842 5842
Size: 5848
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 3655 4037 4672
Size: 53 83 186 936 476 1646 382 635
Cuts at: 4672 275
Size: 1451
Fragments arranged by size:
1646 1451 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4281 4679 4953 5100 5511 4281
Size: 398 274 147 411 4618
Fragments arranged by size:
4618 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5623
Fragments arranged by size:
5623 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2186 1186
Size: 1000 4848
BseYI C'CCAG_C
Cuts at: 5051 5547 5051
Size: 496 5352
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2261 1163
Size: 573 525 4750
Fragments arranged by size:
4750 573 525
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3298 4059 5087 1540
Size: 275 186 1297 761 1028 2301
Fragments arranged by size:
2301 1297 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3085 3909 3994 5065 3085
Size: 824 85 1071 3868
Fragments arranged by size:
3868 1071 824 85
BsmI GAATG_Cn'
Cuts at: 3359 3452 3359
Size: 93 5755
BsmBI CGTCTCn'nnnn_
Cuts at: 2016 2016
Size: 5848
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3085 3167 3909 3994 5065 1391
Size: 399 1295 82 742 85 1071 2174
Fragments arranged by size:
2174 1295 1071 742 399 85 82
BspEI T'CCGG_A
Cuts at: 5511 5511
Size: 5848
BspHI T'CATG_A
Cuts at: 3737 5467 3737
Size: 1730 4118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3203 844
Size: 2359 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2026 3735 1394
Size: 429 203 1709 3507
Fragments arranged by size:
3507 1709 429 203
BsrDI GCAATG_nn'
Cuts at: 65 1248 4343 4517 65
Size: 1183 3095 174 1396
Fragments arranged by size:
3095 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4492 4679 1700
Size: 110 2682 187 2869
Fragments arranged by size:
2869 2682 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5848
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5848
BssSI C'ACGA_G
Cuts at: 3908 4920 3908
Size: 1012 4836
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5848
BstBI TT'CG_AA
Cuts at: 4652 4652
Size: 5848
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5848
BstYI r'GATC_y
Cuts at: 1972 2356 2510 3178 3545 3930 3947 5399 5485
Size: 384 154 668 367 385 17 1452 86
Cuts at: 5485 5497 5843 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 668 385 384 367 346 154
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2976 3000 2976
Size: 24 5824
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4357
Fragments arranged by size:
4357 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5109
Fragments arranged by size:
5109 683 56
BtsI GCAGTG_nn'
Cuts at: 3364 4158 4178 3364
Size: 794 20 5034
Fragments arranged by size:
5034 794 20
ClaI AT'CG_AT
Cuts at: 1554 2838 1554
Size: 1284 4564
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5813
DraI TTT'AAA
Cuts at: 3152 3152
Size: 5848
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4855 809
Size: 507 3539 1802
Fragments arranged by size:
3539 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2027 2231 3033
Size: 54 1425 50 275 18 197 204 802
Cuts at: 3033 3295 4184 5813 8
Size: 262 889 1629 43
Fragments arranged by size:
1629 1425 889 802 275 262 204 197
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3295 1537
Size: 275 1483 4090
Fragments arranged by size:
4090 1483 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3778 1353
Size: 2425 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4435 4809 4955 387
Size: 1064 138 2846 374 146 1280
Fragments arranged by size:
2846 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3083 3083
Size: 5848
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2396 3114 3911 4508 5295 5563
Size: 393 410 354 718 797 597 787 268
Cuts at: 5563 1239
Size: 1524
Fragments arranged by size:
1524 797 787 718 597 410 393 354
268
EcoRI G'AATT_C
Cuts at: 2406 3157 3535 2406
Size: 751 378 4719
Fragments arranged by size:
4719 751 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2639 2639
Size: 5848
FspI TGC'GCA
Cuts at: 4354 4354
Size: 5848
HaeII r_GCGC'y
Cuts at: 1678 2043 4995 1678
Size: 365 2952 2531
Fragments arranged by size:
2952 2531 365
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2314 2346 4489 4521 4563
Size: 32 135 32 742 32 2143 32 42
Cuts at: 4563 4595 1373
Size: 32 2626
Fragments arranged by size:
2626 2143 742 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3192 3438 669
Size: 988 281 1254 246 3079
Fragments arranged by size:
3079 1254 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2665 3006 748
Size: 1917 341 3590
Fragments arranged by size:
3590 1917 341
HpaI GTT'AAC
Cuts at: 3438 3438
Size: 5848
KpnI G_GTAC'C
Cuts at: 3173 3173
Size: 5848
MfeI C'AATT_G
Cuts at: 2988 3447 2988
Size: 459 5389
MluI A'CGCG_T
Cuts at: 5724 5724
Size: 5848
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2102 4962 5146 1305
Size: 797 2860 184 2007
Fragments arranged by size:
2860 2007 797 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2233 3035 10
Size: 54 1425 343 401 802 2823
Fragments arranged by size:
2823 1425 802 401 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2268 2751 3806 4165 4324 518
Size: 648 1102 483 1055 359 159 2042
Fragments arranged by size:
2042 1102 1055 648 483 359 159
MspA1I CmG'CkG
Cuts at: 1606 3092 3264 3941 5089 5334 1606
Size: 1486 172 677 1148 245 2120
Fragments arranged by size:
2120 1486 1148 677 245 172
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4357
Fragments arranged by size:
4357 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5848
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5848
NotI GC'GGCC_GC
Cuts at: 3295 3295
Size: 5848
NspI r_CATG'y
Cuts at: 1132 1229 2430 3206 4751 1132
Size: 97 1201 776 1545 2229
Fragments arranged by size:
2229 1545 1201 776 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5848
PciI A'CATG_T
Cuts at: 4747 4747
Size: 5848
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5848
PmeI GTTT'AAAC
Cuts at: 3152 3152
Size: 5848
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3917 3949 5430 5462 3917
Size: 32 1481 32 4303
Fragments arranged by size:
4303 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5848
PsiI TTA'TAA
Cuts at: 2655 3418 2655
Size: 763 5085
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2385 2417 2385
Size: 32 5816
PstI C_TGCA'G
Cuts at: 830 3200 830
Size: 2370 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5848
PvuII CAG'CTG
Cuts at: 3092 3092
Size: 5848
SacI G_AGCT'C
Cuts at: 3085 3085
Size: 5848
SalI G'TCGA_C
Cuts at: 1936 3190 1936
Size: 1254 4594
SbfI CC_TGCA'GG
Cuts at: 3200 3200
Size: 5848
ScaI AGT'ACT
Cuts at: 4096 5808 4096
Size: 1712 4136
SfcI C'TryA_G
Cuts at: 826 1045 3196 4331 5012 5203 826
Size: 219 2151 1135 681 191 1471
Fragments arranged by size:
2151 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5848
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5848
SmaI CCC'GGG
Cuts at: 1872 3175 1872
Size: 1303 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3107 3952 4853 5115 820
Size: 197 926 1164 845 901 262 1553
Fragments arranged by size:
1553 1164 926 901 845 262 197
SnaBI TAC'GTA
Cuts at: 493 3639 493
Size: 3146 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5848
SphI G_CATG'C
Cuts at: 1132 3206 1132
Size: 2074 3774
SspI AAT'ATT
Cuts at: 5 52 2641 3772 5
Size: 47 2589 1131 2081
Fragments arranged by size:
2589 2081 1131 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5848
StyI C'CwwG_G
Cuts at: 513 1065 2004 3287 513
Size: 552 939 1283 3074
Fragments arranged by size:
3074 1283 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3875 3892 4045 4230 4675 325
Size: 1255 2295 17 153 185 445 1498
Fragments arranged by size:
2295 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2309 4094 5806
Size: 274 80 33 51 1443 332 1785 1712
Cuts at: 5806 96
Size: 138
Fragments arranged by size:
1785 1712 1443 332 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5808 5840 5808
Size: 32 5816
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5848
XbaI T'CTAG_A
Cuts at: 3184 3184
Size: 5848
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2164 2164
Size: 5848
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5848
XmaI C'CCGG_G
Cuts at: 1870 3173 1870
Size: 1303 4545
XmnI GAAnn'nnTTC
Cuts at: 3977 3977
Size: 5848
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3656 276
Size: 53 83 186 936 2122 2468
Fragments arranged by size:
2468 2122 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I DraIII
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI