Restriction map for
pFN21AE5557
(Circular) (Six-base) MAPSORT of: pFN21AE5557.seq Check: 2900 from: 1 to: 5836
pFN21AE5557 5836 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2976 2976
Size: 5836
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3646 278
Size: 53 83 186 936 2110 2468
Fragments arranged by size:
2468 2110 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3179 1937
Size: 1242 4594
Acc65I G'GTAC_C
Cuts at: 3157 3157
Size: 5836
AclI AA'CG_TT
Cuts at: 1563 3963 4336 1563
Size: 2400 373 3063
Fragments arranged by size:
3063 2400 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2939 3899 5283 5551 1632
Size: 1307 960 1384 268 1917
Fragments arranged by size:
1917 1384 1307 960 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5639
AflIII A'CryG_T
Cuts at: 1203 4735 5712 1203
Size: 3532 977 1327
Fragments arranged by size:
3532 1327 977
AgeI A'CCGG_T
Cuts at: 4667 4667
Size: 5836
AhdI GACnn_n'nnGTC
Cuts at: 4565 4565
Size: 5836
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5836
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5836
AlwNI CAG_nnn'CTG
Cuts at: 2902 3579 5151 2902
Size: 677 1572 3587
Fragments arranged by size:
3587 1572 677
ApaI G_GGCC'C
Cuts at: 2036 2036
Size: 5836
ApaLI G'TGCA_C
Cuts at: 3893 5049 3893
Size: 1156 4680
ApoI r'AATT_y
Cuts at: 1493 2298 3145 3371 3523 5632 1493
Size: 805 847 226 152 2109 1697
Fragments arranged by size:
2109 1697 847 805 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4390 160
Size: 872 3358 1606
Fragments arranged by size:
3358 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
AvaI C'yCGr_G
Cuts at: 1870 1943 2292 2668 3161 1870
Size: 73 349 376 493 4545
Fragments arranged by size:
4545 493 376 349 73
BamHI G'GATC_C
Cuts at: 2040 3166 3533 2040
Size: 1126 367 4343
Fragments arranged by size:
4343 1126 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2011 2912 3157 4612 618
Size: 325 217 627 224 901 245 1455 1842
Fragments arranged by size:
1842 1455 901 627 325 245 224 217
BanII G_rGCy'C
Cuts at: 1391 2036 2161 2439 2787 3155 1391
Size: 645 125 278 348 368 4072
Fragments arranged by size:
4072 645 368 348 278 125
BbeI G_GCGC'C
Cuts at: 2015 2015
Size: 5836
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4865
BbvCI CC'TCA_GC
Cuts at: 2590 2590
Size: 5836
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4027 4061 1903
Size: 34 2090 34 3678
Fragments arranged by size:
3678 2090 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3728 4938 3728
Size: 1210 4626
BclI T'GATC_A
Cuts at: 1548 2860 1548
Size: 1312 4524
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2120 4447
Size: 107 122 71 631 605 337 111 2327
Cuts at: 4447 136
Size: 1525
Fragments arranged by size:
2327 1525 631 605 337 122 111 107
71
BglII A'GATC_T
Cuts at: 5831 5831
Size: 5836
BlpI GC'TnA_GC
Cuts at: 2753 3253 2753
Size: 500 5336
Bme1580I G_kGCm'C
Cuts at: 1790 2036 2915 3897 5053 1790
Size: 246 879 982 1156 2573
Fragments arranged by size:
2573 1156 982 879 246
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5836
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2569 3567 4525
Size: 416 53 83 399 24 1152 998 958
Cuts at: 4525 442
Size: 1753
Fragments arranged by size:
1753 1152 998 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5836
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2318 2318
Size: 5836
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2771 4496 1239
Size: 1532 1725 2579
Fragments arranged by size:
2579 1725 1532
Bpu10I CC'TnA_GC
Cuts at: 2280 2590 2280
Size: 310 5526
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3961 4826 5124 3961
Size: 865 298 4673
Fragments arranged by size:
4673 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2351 4499 882
Size: 617 852 2148 2219
Fragments arranged by size:
2219 2148 852 617
BsaAI yAC'GTr
Cuts at: 493 1204 3627 493
Size: 711 2423 2702
Fragments arranged by size:
2702 2423 711
BsaBI GATnn'nnATC
Cuts at: 5830 5830
Size: 5836
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 3643 4025 4660
Size: 53 83 186 936 479 1631 382 635
Cuts at: 4660 275
Size: 1451
Fragments arranged by size:
1631 1451 936 635 479 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4269 4667 4941 5088 5499 4269
Size: 398 274 147 411 4606
Fragments arranged by size:
4606 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2180 2210 3120 3150 1178
Size: 30 165 30 777 30 910 30 3864
Fragments arranged by size:
3864 910 777 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2758 1186
Size: 1572 4264
BseYI C'CCAG_C
Cuts at: 2926 5039 5535 2926
Size: 2113 496 3227
Fragments arranged by size:
3227 2113 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2954 3047 1163
Size: 573 1218 93 3952
Fragments arranged by size:
3952 1218 573 93
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3286 4047 5075 1540
Size: 275 186 1285 761 1028 2301
Fragments arranged by size:
2301 1285 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2754 2787 3133 3897 3982 5053 2754
Size: 33 346 764 85 1071 3537
Fragments arranged by size:
3537 1071 764 346 85 33
BsmI GAATG_Cn'
Cuts at: 2725 3055 3347 3440 2725
Size: 330 292 93 5121
Fragments arranged by size:
5121 330 292 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2089 2089
Size: 5836
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2161 2439 2754 2787 2915 3133
Size: 399 246 125 278 315 33 128 218
Cuts at: 3133 3155 3897 3982 5053 1391
Size: 22 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 315 278 246 218
128 125 85 33 22
BspEI T'CCGG_A
Cuts at: 5499 5499
Size: 5836
BspHI T'CATG_A
Cuts at: 3725 5455 3725
Size: 1730 4106
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2976 3191 844
Size: 2132 215 3489
Fragments arranged by size:
3489 2132 215
BsrBI CCG'CTC
Cuts at: 1394 1823 3723 1394
Size: 429 1900 3507
Fragments arranged by size:
3507 1900 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4331 4505 65
Size: 1183 3083 174 1396
Fragments arranged by size:
3083 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2191 2454 4480 4667 1700
Size: 110 381 263 2026 187 2869
Fragments arranged by size:
2869 2026 381 263 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5836
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5836
BssSI C'ACGA_G
Cuts at: 2777 3896 4908 2777
Size: 1119 1012 3705
Fragments arranged by size:
3705 1119 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3061 3070 794
Size: 2267 9 3560
Fragments arranged by size:
3560 2267 9
BstBI TT'CG_AA
Cuts at: 4640 4640
Size: 5836
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5836
BstXI CCAn_nnnn'nTGG
Cuts at: 3124 3124
Size: 5836
BstYI r'GATC_y
Cuts at: 1972 2040 3166 3533 3918 3935 5387 5473 5485
Size: 68 1126 367 385 17 1452 86 12
Cuts at: 5485 5831 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1126 385 367 346 86 68
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4345
Fragments arranged by size:
4345 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5097
Fragments arranged by size:
5097 683 56
BtsI GCAGTG_nn'
Cuts at: 3352 4146 4166 3352
Size: 794 20 5022
Fragments arranged by size:
5022 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5836
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5801
DraI TTT'AAA
Cuts at: 3140 3140
Size: 5836
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4843 809
Size: 507 3527 1802
Fragments arranged by size:
3527 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2053 2121 2422
Size: 54 1425 50 275 18 223 68 301
Cuts at: 2422 3283 4172 5801 8
Size: 861 889 1629 43
Fragments arranged by size:
1629 1425 889 861 301 275 223 68
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3283 1537
Size: 275 1471 4090
Fragments arranged by size:
4090 1471 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2078 3766 1353
Size: 725 1688 3423
Fragments arranged by size:
3423 1688 725
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2761 4423 4797 4943 387
Size: 1064 138 1172 1662 374 146 1280
Fragments arranged by size:
1662 1280 1172 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2785 2785
Size: 5836
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2771 2939 3899 4496 5283 5551 1239
Size: 393 1139 168 960 597 787 268 1524
Fragments arranged by size:
1524 1139 960 787 597 393 268 168
EcoO109I rG'GnC_Cy
Cuts at: 2033 2033
Size: 5836
EcoRI G'AATT_C
Cuts at: 2298 3145 3523 2298
Size: 847 378 4611
Fragments arranged by size:
4611 847 378
FspI TGC'GCA
Cuts at: 2724 4342 2724
Size: 1618 4218
HaeII r_GCGC'y
Cuts at: 1678 2015 4983 1678
Size: 337 2968 2531
Fragments arranged by size:
2968 2531 337
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2180 2212 4477 4509 4551
Size: 32 135 32 608 32 2265 32 42
Cuts at: 4551 4583 1373
Size: 32 2626
Fragments arranged by size:
2626 2265 608 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3180 3426 669
Size: 988 281 1242 246 3079
Fragments arranged by size:
3079 1242 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5836
HpaI GTT'AAC
Cuts at: 3426 3426
Size: 5836
KasI G'GCGC_C
Cuts at: 2011 2011
Size: 5836
KpnI G_GTAC'C
Cuts at: 3161 3161
Size: 5836
MfeI C'AATT_G
Cuts at: 3435 3435
Size: 5836
MluI A'CGCG_T
Cuts at: 5712 5712
Size: 5836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4950 5134 1305
Size: 3645 184 2007
Fragments arranged by size:
3645 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2424 10
Size: 54 1425 343 592 3422
Fragments arranged by size:
3422 1425 592 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2570 3794 4153 4312 518
Size: 648 1404 1224 359 159 2042
Fragments arranged by size:
2042 1404 1224 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2105 2111 2215 2251 2983 3252 3929 5077
Size: 499 6 104 36 732 269 677 1148
Cuts at: 5077 5322 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 732 677 499 269 245 104
36 6
NarI GG'CG_CC
Cuts at: 2012 2012
Size: 5836
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4345
Fragments arranged by size:
4345 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5836
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5836
NotI GC'GGCC_GC
Cuts at: 3283 3283
Size: 5836
NspI r_CATG'y
Cuts at: 1132 1229 2579 3194 4739 1132
Size: 97 1350 615 1545 2229
Fragments arranged by size:
2229 1545 1350 615 97
PasI CC'CwG_GG
Cuts at: 1348 2366 2998 1348
Size: 1018 632 4186
Fragments arranged by size:
4186 1018 632
PciI A'CATG_T
Cuts at: 4735 4735
Size: 5836
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2458 2855 1108
Size: 1350 397 4089
Fragments arranged by size:
4089 1350 397
PfoI T'CCnGG_A
Cuts at: 3005 3005
Size: 5836
PmeI GTTT'AAAC
Cuts at: 3140 3140
Size: 5836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3905 3937 5418 5450 3905
Size: 32 1481 32 4291
Fragments arranged by size:
4291 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5836
PsiI TTA'TAA
Cuts at: 3406 3406
Size: 5836
PspOMI G'GGCC_C
Cuts at: 2032 2032
Size: 5836
PstI C_TGCA'G
Cuts at: 830 2256 3188 830
Size: 1426 932 3478
Fragments arranged by size:
3478 1426 932
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5836
PvuII CAG'CTG
Cuts at: 2251 2983 2251
Size: 732 5104
SacI G_AGCT'C
Cuts at: 2787 2787
Size: 5836
SalI G'TCGA_C
Cuts at: 1936 3178 1936
Size: 1242 4594
SbfI CC_TGCA'GG
Cuts at: 3188 3188
Size: 5836
ScaI AGT'ACT
Cuts at: 2558 2870 4084 5796 2558
Size: 312 1214 1712 2598
Fragments arranged by size:
2598 1712 1214 312
SexAI A'CCwGG_T
Cuts at: 2745 2745
Size: 5836
SfcI C'TryA_G
Cuts at: 826 1045 2252 3184 4319 5000 5191 826
Size: 219 1207 932 1135 681 191 1471
Fragments arranged by size:
1471 1207 1135 932 681 219 191
SfoI GGC'GCC
Cuts at: 2013 2013
Size: 5836
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5836
SmaI CCC'GGG
Cuts at: 1872 3163 1872
Size: 1291 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3940 4841 5103 820
Size: 197 926 1997 901 262 1553
Fragments arranged by size:
1997 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3627 493
Size: 3134 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5836
SphI G_CATG'C
Cuts at: 1132 3194 1132
Size: 2062 3774
SspI AAT'ATT
Cuts at: 5 52 3760 5
Size: 47 3708 2081
Fragments arranged by size:
3708 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5836
StyI C'CwwG_G
Cuts at: 513 1065 2004 2056 2854 3275 513
Size: 552 939 52 798 421 3074
Fragments arranged by size:
3074 939 798 552 421 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3863 3880 4033 4218 4663 325
Size: 1255 2283 17 153 185 445 1498
Fragments arranged by size:
2283 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2392 2556 2868
Size: 274 80 33 51 1443 415 164 312
Cuts at: 2868 4082 5794 96
Size: 1214 1712 138
Fragments arranged by size:
1712 1443 1214 415 312 274 164 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2502 2534 5796 5828 2502
Size: 32 3262 32 2510
Fragments arranged by size:
3262 2510 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2662 1316
Size: 1346 4490
XbaI T'CTAG_A
Cuts at: 3172 3172
Size: 5836
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2479 2795 2479
Size: 316 5520
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5836
XmaI C'CCGG_G
Cuts at: 1870 3161 1870
Size: 1291 4545
XmnI GAAnn'nnTTC
Cuts at: 3965 3965
Size: 5836
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3644 276
Size: 53 83 186 936 2110 2468
Fragments arranged by size:
2468 2110 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI
RsrII SacII SanDI SapI SfiI SrfI SwaI