Restriction map for
pFN21AE5555
(Circular) (Six-base) MAPSORT of: pFN21AE5555.seq Check: 6642 from: 1 to: 5806
pFN21AE5555 5806 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3616 278
Size: 53 83 186 936 2080 2468
Fragments arranged by size:
2468 2080 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3149 1937
Size: 1212 4594
Acc65I G'GTAC_C
Cuts at: 2870 3127 2870
Size: 257 5549
AclI AA'CG_TT
Cuts at: 1563 2016 3933 4306 1563
Size: 453 1917 373 3063
Fragments arranged by size:
3063 1917 453 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3869 5253 5521 1632
Size: 2237 1384 268 1917
Fragments arranged by size:
2237 1917 1384 268
AfeI AGC'GCT
Cuts at: 3016 3016
Size: 5806
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5609
AflIII A'CryG_T
Cuts at: 1203 4705 5682 1203
Size: 3502 977 1327
Fragments arranged by size:
3502 1327 977
AgeI A'CCGG_T
Cuts at: 2485 4637 2485
Size: 2152 3654
AhdI GACnn_n'nnGTC
Cuts at: 4535 4535
Size: 5806
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5806
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2295 1184
Size: 1111 4695
AlwNI CAG_nnn'CTG
Cuts at: 3549 5121 3549
Size: 1572 4234
ApaLI G'TGCA_C
Cuts at: 3863 5019 3863
Size: 1156 4650
ApoI r'AATT_y
Cuts at: 1493 2108 2445 3115 3341 3493 5602 1493
Size: 615 337 670 226 152 2109 1697
Fragments arranged by size:
2109 1697 670 615 337 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2247 4360 160
Size: 872 1215 2113 1606
Fragments arranged by size:
2113 1606 1215 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5806
AvaI C'yCGr_G
Cuts at: 1870 1943 2497 2577 3131 1870
Size: 73 554 80 554 4545
Fragments arranged by size:
4545 554 554 80 73
BamHI G'GATC_C
Cuts at: 3136 3503 3136
Size: 367 5439
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2870 3127 4582 618
Size: 325 217 627 1083 257 1455 1842
Fragments arranged by size:
1842 1455 1083 627 325 257 217
BanII G_rGCy'C
Cuts at: 1391 2504 3125 1391
Size: 1113 621 4072
Fragments arranged by size:
4072 1113 621
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4835
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2608 2642 3997 4031 1903
Size: 34 671 34 1355 34 3678
Fragments arranged by size:
3678 1355 671 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3698 4908 3698
Size: 1210 4596
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5806
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2780 4417 136
Size: 107 122 71 631 605 1108 1637 1525
Fragments arranged by size:
1637 1525 1108 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5801 5801
Size: 5806
BlpI GC'TnA_GC
Cuts at: 3223 3223
Size: 5806
Bme1580I G_kGCm'C
Cuts at: 1790 2346 2578 3867 5023 1790
Size: 556 232 1289 1156 2573
Fragments arranged by size:
2573 1289 1156 556 232
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5806
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2018 3537 4495
Size: 416 53 83 399 24 601 1519 958
Cuts at: 4495 442
Size: 1753
Fragments arranged by size:
1753 1519 958 601 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5806
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2442 2561 4466 1239
Size: 1203 119 1905 2579
Fragments arranged by size:
2579 1905 1203 119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3931 4796 5094 3931
Size: 865 298 4643
Fragments arranged by size:
4643 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2579 4469 882
Size: 617 1080 1890 2219
Fragments arranged by size:
2219 1890 1080 617
BsaAI yAC'GTr
Cuts at: 493 1204 3597 493
Size: 711 2393 2702
Fragments arranged by size:
2702 2393 711
BsaBI GATnn'nnATC
Cuts at: 5800 5800
Size: 5806
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3613 3995 4630 275
Size: 53 83 186 936 2080 382 635 1451
Fragments arranged by size:
2080 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2485 4239 4637 4911 5058 5469 2485
Size: 1754 398 274 147 411 2822
Fragments arranged by size:
2822 1754 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5581
Fragments arranged by size:
5581 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5806
BseYI C'CCAG_C
Cuts at: 2298 2891 5009 5505 2298
Size: 593 2118 496 2599
Fragments arranged by size:
2599 2118 593 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2700 1163
Size: 573 964 4269
Fragments arranged by size:
4269 964 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2793 3256 4017 5045 1540
Size: 275 186 792 463 761 1028 2301
Fragments arranged by size:
2301 1028 792 761 463 275 186
BsiHKAI G_wGCw'C
Cuts at: 2827 3867 3952 5023 2827
Size: 1040 85 1071 3610
Fragments arranged by size:
3610 1071 1040 85
BsmI GAATG_Cn'
Cuts at: 2108 3317 3410 2108
Size: 1209 93 4504
Fragments arranged by size:
4504 1209 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2346 2504 2578 2827 3125 3867 3952
Size: 399 556 158 74 249 298 742 85
Cuts at: 3952 5023 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 556 399 298 249 158
85 74
BspEI T'CCGG_A
Cuts at: 5469 5469
Size: 5806
BspHI T'CATG_A
Cuts at: 3695 5425 3695
Size: 1730 4076
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3161 844
Size: 2317 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3693 1394
Size: 429 1870 3507
Fragments arranged by size:
3507 1870 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2295 2872 4301 4475 65
Size: 1183 1047 577 1429 174 1396
Fragments arranged by size:
1429 1396 1183 1047 577 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2485 4450 4637 1700
Size: 110 675 1965 187 2869
Fragments arranged by size:
2869 1965 675 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5806
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5806
BssSI C'ACGA_G
Cuts at: 3866 4878 3866
Size: 1012 4794
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2294 794
Size: 1500 4306
BstBI TT'CG_AA
Cuts at: 4610 4610
Size: 5806
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5806
BstXI CCAn_nnnn'nTGG
Cuts at: 2806 2806
Size: 5806
BstYI r'GATC_y
Cuts at: 1972 3136 3503 3888 3905 5357 5443 5455 5801
Size: 1164 367 385 17 1452 86 12 346
Cuts at: 5801 1972
Size: 1977
Fragments arranged by size:
1977 1452 1164 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2787 2793 513
Size: 552 939 783 6 3526
Fragments arranged by size:
3526 939 783 552 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5067
Fragments arranged by size:
5067 683 56
BtsI GCAGTG_nn'
Cuts at: 3322 4116 4136 3322
Size: 794 20 4992
Fragments arranged by size:
4992 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5806
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5771
DraI TTT'AAA
Cuts at: 3110 3110
Size: 5806
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4813 809
Size: 507 3497 1802
Fragments arranged by size:
3497 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2772 2790 2796
Size: 54 1425 50 275 18 942 18 6
Cuts at: 2796 3253 4142 5771 8
Size: 457 889 1629 43
Fragments arranged by size:
1629 1425 942 889 457 275 54 50
43 18 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2790 3253 1537
Size: 275 978 463 4090
Fragments arranged by size:
4090 978 463 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2143 2832 3736 1353
Size: 790 689 904 3423
Fragments arranged by size:
3423 904 790 689
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3013 4393 4767 4913 387
Size: 1064 138 1424 1380 374 146 1280
Fragments arranged by size:
1424 1380 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2442 2561 3869 4466 5253 5521 1239
Size: 393 810 119 1308 597 787 268 1524
Fragments arranged by size:
1524 1308 810 787 597 393 268 119
EcoO109I rG'GnC_Cy
Cuts at: 2360 2360
Size: 5806
EcoRI G'AATT_C
Cuts at: 3115 3493 3115
Size: 378 5428
FspI TGC'GCA
Cuts at: 2745 4312 2745
Size: 1567 4239
HaeII r_GCGC'y
Cuts at: 1678 3018 4953 1678
Size: 1340 1935 2531
Fragments arranged by size:
2531 1935 1340
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4447 4479 4521 4553 1373
Size: 32 135 32 2875 32 42 32 2626
Fragments arranged by size:
2875 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3150 3396 669
Size: 988 281 1212 246 3079
Fragments arranged by size:
3079 1212 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5806
HpaI GTT'AAC
Cuts at: 3396 3396
Size: 5806
KpnI G_GTAC'C
Cuts at: 2874 3131 2874
Size: 257 5549
MfeI C'AATT_G
Cuts at: 3405 3405
Size: 5806
MluI A'CGCG_T
Cuts at: 5682 5682
Size: 5806
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2243 2981 4920 5104 1305
Size: 938 738 1939 184 2007
Fragments arranged by size:
2007 1939 938 738 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3984
Fragments arranged by size:
3984 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2804 3764 4123 4282 518
Size: 648 1638 960 359 159 2042
Fragments arranged by size:
2042 1638 960 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2789 2795 3222 3899 5047 5292 1606
Size: 1183 6 427 677 1148 245 2120
Fragments arranged by size:
2120 1183 1148 677 427 245 6
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4315
Fragments arranged by size:
4315 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5806
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5806
NotI GC'GGCC_GC
Cuts at: 2790 3253 2790
Size: 463 5343
NspI r_CATG'y
Cuts at: 1132 1229 2085 3164 4709 1132
Size: 97 856 1079 1545 2229
Fragments arranged by size:
2229 1545 1079 856 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5806
PciI A'CATG_T
Cuts at: 4705 4705
Size: 5806
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5806
PfoI T'CCnGG_A
Cuts at: 2320 2473 2320
Size: 153 5653
PmeI GTTT'AAAC
Cuts at: 3110 3110
Size: 5806
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3875 3907 5388 5420 3875
Size: 32 1481 32 4261
Fragments arranged by size:
4261 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2360 2360
Size: 5806
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5806
PsiI TTA'TAA
Cuts at: 3376 3376
Size: 5806
PstI C_TGCA'G
Cuts at: 830 2063 2284 3040 3158 830
Size: 1233 221 756 118 3478
Fragments arranged by size:
3478 1233 756 221 118
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5806
SacII CC_GC'GG
Cuts at: 2790 2796 2790
Size: 6 5800
SalI G'TCGA_C
Cuts at: 1936 3148 1936
Size: 1212 4594
SapI GCTCTTCn'nnn_
Cuts at: 2832 2832
Size: 5806
SbfI CC_TGCA'GG
Cuts at: 3158 3158
Size: 5806
ScaI AGT'ACT
Cuts at: 4054 5766 4054
Size: 1712 4094
SfcI C'TryA_G
Cuts at: 826 1045 2059 2280 2355 3036 3154 4289 4970
Size: 219 1014 221 75 681 118 1135 681
Cuts at: 4970 5161 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1014 681 681 221 219 191
118 75
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5806
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5806
SmaI CCC'GGG
Cuts at: 1872 2499 2579 3133 1872
Size: 627 80 554 4545
Fragments arranged by size:
4545 627 554 80
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3910 4811 5073 820
Size: 197 926 1967 901 262 1553
Fragments arranged by size:
1967 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3597 493
Size: 3104 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5806
SphI G_CATG'C
Cuts at: 1132 3164 1132
Size: 2032 3774
SspI AAT'ATT
Cuts at: 5 52 2702 3730 5
Size: 47 2650 1028 2081
Fragments arranged by size:
2650 2081 1028 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5806
StyI C'CwwG_G
Cuts at: 513 1065 2004 3245 513
Size: 552 939 1241 3074
Fragments arranged by size:
3074 1241 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2603 3833 3850 4003 4188 4633 325
Size: 1255 1023 1230 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1230 1023 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4052 5764 96
Size: 274 80 33 51 1443 2075 1712 138
Fragments arranged by size:
2075 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5766 5798 5766
Size: 32 5774
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5806
XbaI T'CTAG_A
Cuts at: 3142 3142
Size: 5806
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5806
XmaI C'CCGG_G
Cuts at: 1870 2497 2577 3131 1870
Size: 627 80 554 4545
Fragments arranged by size:
4545 627 554 80
XmnI GAAnn'nnTTC
Cuts at: 3935 3935
Size: 5806
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3614 276
Size: 53 83 186 936 2080 2468
Fragments arranged by size:
2468 2080 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SanDI SexAI SfiI SfoI
SrfI SwaI XcmI