Restriction map for
pFN21AE5554
(Circular) (Six-base) MAPSORT of: pFN21AE5554.seq Check: 1731 from: 1 to: 5992
pFN21AE5554 5992 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2053 2053
Size: 5992
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3802 278
Size: 53 83 186 936 2266 2468
Fragments arranged by size:
2468 2266 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3335 1937
Size: 1398 4594
Acc65I G'GTAC_C
Cuts at: 3313 3313
Size: 5992
AclI AA'CG_TT
Cuts at: 1563 4119 4492 1563
Size: 2556 373 3063
Fragments arranged by size:
3063 2556 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2244 4055 5439 5707 1632
Size: 612 1811 1384 268 1917
Fragments arranged by size:
1917 1811 1384 612 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5795
AflIII A'CryG_T
Cuts at: 1203 4891 5868 1203
Size: 3688 977 1327
Fragments arranged by size:
3688 1327 977
AgeI A'CCGG_T
Cuts at: 4823 4823
Size: 5992
AhdI GACnn_n'nnGTC
Cuts at: 2657 4721 2657
Size: 2064 3928
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5992
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5992
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2040 2072 2040
Size: 32 5960
AlwNI CAG_nnn'CTG
Cuts at: 2068 2078 2882 3068 3735 5307 2068
Size: 10 804 186 667 1572 2753
Fragments arranged by size:
2753 1572 804 667 186 10
ApaI G_GGCC'C
Cuts at: 2380 2757 2849 2380
Size: 377 92 5523
Fragments arranged by size:
5523 377 92
ApaLI G'TGCA_C
Cuts at: 4049 5205 4049
Size: 1156 4836
ApoI r'AATT_y
Cuts at: 1493 2922 3301 3527 3679 5788 1493
Size: 1429 379 226 152 2109 1697
Fragments arranged by size:
2109 1697 1429 379 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4546 160
Size: 872 3514 1606
Fragments arranged by size:
3514 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5992
AvaI C'yCGr_G
Cuts at: 1870 1943 2026 2990 3138 3317 1870
Size: 73 83 964 148 179 4545
Fragments arranged by size:
4545 964 179 148 83 73
BamHI G'GATC_C
Cuts at: 3322 3689 3322
Size: 367 5625
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2454 2707 3313 4768 618
Size: 325 217 627 667 253 606 1455 1842
Fragments arranged by size:
1842 1455 667 627 606 325 253 217
BanII G_rGCy'C
Cuts at: 1391 2139 2380 2757 2849 3311 1391
Size: 748 241 377 92 462 4072
Fragments arranged by size:
4072 748 462 377 241 92
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2343 928
Size: 971 444 4577
Fragments arranged by size:
4577 971 444
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2219 2253 2831 2865 4183 4217 1903
Size: 34 282 34 578 34 1318 34 3678
Fragments arranged by size:
3678 1318 578 282 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3884 5094 3884
Size: 1210 4782
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5992
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4603 136
Size: 107 122 71 631 605 2931 1525
Fragments arranged by size:
2931 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5987 5987
Size: 5992
BlpI GC'TnA_GC
Cuts at: 3409 3409
Size: 5992
Bme1580I G_kGCm'C
Cuts at: 1790 2380 2757 2849 4053 5209 1790
Size: 590 377 92 1204 1156 2573
Fragments arranged by size:
2573 1204 1156 590 377 92
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5992
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2408 2870 2922
Size: 416 53 83 399 24 991 462 52
Cuts at: 2922 3723 4681 442
Size: 801 958 1753
Fragments arranged by size:
1753 991 958 801 462 416 399 83
53 52 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5992
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2098 2098
Size: 5992
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2647 3090 4652 1239
Size: 1408 443 1562 2579
Fragments arranged by size:
2579 1562 1408 443
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4117 4982 5280 4117
Size: 865 298 4829
Fragments arranged by size:
4829 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2294 4655 882
Size: 617 795 2361 2219
Fragments arranged by size:
2361 2219 795 617
BsaAI yAC'GTr
Cuts at: 493 1204 3783 493
Size: 711 2579 2702
Fragments arranged by size:
2702 2579 711
BsaBI GATnn'nnATC
Cuts at: 5986 5986
Size: 5992
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2832 2997 3799 4181
Size: 53 83 186 936 1299 165 802 382
Cuts at: 4181 4816 275
Size: 635 1451
Fragments arranged by size:
1451 1299 936 802 635 382 186 165
83 53
BsaWI w'CCGG_w
Cuts at: 4425 4823 5097 5244 5655 4425
Size: 398 274 147 411 4762
Fragments arranged by size:
4762 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2679 2709 1178
Size: 30 165 30 1276 30 4461
Fragments arranged by size:
4461 1276 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3069 1186
Size: 1883 4109
BseYI C'CCAG_C
Cuts at: 2100 2274 2800 3164 5195 5691 2100
Size: 174 526 364 2031 496 2401
Fragments arranged by size:
2401 2031 526 496 364 174
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2816 1163
Size: 573 1080 4339
Fragments arranged by size:
4339 1080 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3442 4203 5231 1540
Size: 275 186 1441 761 1028 2301
Fragments arranged by size:
2301 1441 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2139 4053 4138 5209 2139
Size: 1914 85 1071 2922
Fragments arranged by size:
2922 1914 1071 85
BsmI GAATG_Cn'
Cuts at: 2120 3503 3596 2120
Size: 1383 93 4516
Fragments arranged by size:
4516 1383 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2782 2782
Size: 5992
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2139 2380 2757 2849 3311 4053 4138
Size: 399 349 241 377 92 462 742 85
Cuts at: 4138 5209 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 462 399 377 349 241
92 85
BspEI T'CCGG_A
Cuts at: 5655 5655
Size: 5992
BspHI T'CATG_A
Cuts at: 3881 5611 3881
Size: 1730 4262
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2053 3086 3347 844
Size: 1209 1033 261 3489
Fragments arranged by size:
3489 1209 1033 261
BsrBI CCG'CTC
Cuts at: 1394 1823 2776 3879 1394
Size: 429 953 1103 3507
Fragments arranged by size:
3507 1103 953 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2601 2818 4487 4661 65
Size: 1183 1353 217 1669 174 1396
Fragments arranged by size:
1669 1396 1353 1183 217 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2681 2894 4636 4823 1700
Size: 110 871 213 1742 187 2869
Fragments arranged by size:
2869 1742 871 213 187 110
BsrGI T'GTAC_A
Cuts at: 96 2436 96
Size: 2340 3652
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5992
BssSI C'ACGA_G
Cuts at: 3263 4052 5064 3263
Size: 789 1012 4191
Fragments arranged by size:
4191 1012 789
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5992
BstBI TT'CG_AA
Cuts at: 4796 4796
Size: 5992
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5992
BstYI r'GATC_y
Cuts at: 1972 3322 3689 4074 4091 5543 5629 5641 5987
Size: 1350 367 385 17 1452 86 12 346
Cuts at: 5987 1972
Size: 1977
Fragments arranged by size:
1977 1452 1350 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2946 2946
Size: 5992
BtgI C'CryG_G
Cuts at: 513 1065 2004 2827 513
Size: 552 939 823 3678
Fragments arranged by size:
3678 939 823 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2371 487
Size: 683 56 1145 4108
Fragments arranged by size:
4108 1145 683 56
BtsI GCAGTG_nn'
Cuts at: 2886 3508 4302 4322 2886
Size: 622 794 20 4556
Fragments arranged by size:
4556 794 622 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5992
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5957
DraI TTT'AAA
Cuts at: 3296 3296
Size: 5992
DraIII CAC_nnn'GTG
Cuts at: 2202 2714 3247 2202
Size: 512 533 4947
Fragments arranged by size:
4947 533 512
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4999 809
Size: 507 3683 1802
Fragments arranged by size:
3683 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3439 4328 5957
Size: 54 1425 50 275 18 1609 889 1629
Cuts at: 5957 8
Size: 43
Fragments arranged by size:
1629 1609 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3439 1537
Size: 275 1627 4090
Fragments arranged by size:
4090 1627 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2441 2515 3922 1353
Size: 1088 74 1407 3423
Fragments arranged by size:
3423 1407 1088 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2668 4579 4953 5099 387
Size: 1064 138 1079 1911 374 146 1280
Fragments arranged by size:
1911 1280 1079 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2137 2137
Size: 5992
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2244 2647 3090 4055 4652 5439 5707
Size: 393 612 403 443 965 597 787 268
Cuts at: 5707 1239
Size: 1524
Fragments arranged by size:
1524 965 787 612 597 443 403 393
268
EcoO109I rG'GnC_Cy
Cuts at: 2234 2271 2376 2377 2733 2753 2754 2845 2846
Size: 37 105 1 356 20 1 91 1
Cuts at: 2846 2234
Size: 5380
Fragments arranged by size:
5380 356 105 91 37 20 1 1
1
EcoRI G'AATT_C
Cuts at: 2922 3301 3679 2922
Size: 379 378 5235
Fragments arranged by size:
5235 379 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2060 2060
Size: 5992
FspI TGC'GCA
Cuts at: 4498 4498
Size: 5992
HaeII r_GCGC'y
Cuts at: 1678 5139 1678
Size: 3461 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2462 2494 2643 2675 3104
Size: 32 135 32 890 32 149 32 429
Cuts at: 3104 3136 3242 3274 4633 4665 4707 4739 1373
Size: 32 106 32 1359 32 42 32 2626
Fragments arranged by size:
2626 1359 890 429 149 135 106 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3336 3582 669
Size: 988 281 1398 246 3079
Fragments arranged by size:
3079 1398 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2904 748
Size: 2156 3836
HpaI GTT'AAC
Cuts at: 3582 3582
Size: 5992
KpnI G_GTAC'C
Cuts at: 3317 3317
Size: 5992
MfeI C'AATT_G
Cuts at: 3591 3591
Size: 5992
MluI A'CGCG_T
Cuts at: 5868 5868
Size: 5992
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5106 5290 1305
Size: 3801 184 2007
Fragments arranged by size:
3801 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4170
Fragments arranged by size:
4170 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2768 2811 3950 4309 4468 518
Size: 648 1602 43 1139 359 159 2042
Fragments arranged by size:
2042 1602 1139 648 359 159 43
MspA1I CmG'CkG
Cuts at: 1606 2104 2592 2800 3408 4085 5233 5478 1606
Size: 498 488 208 608 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 608 498 488 245 208
NaeI GCC'GGC
Cuts at: 2896 2896
Size: 5992
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4501
Fragments arranged by size:
4501 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5992
NgoMIV G'CCGG_C
Cuts at: 2894 2894
Size: 5992
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5992
NotI GC'GGCC_GC
Cuts at: 3439 3439
Size: 5992
NspI r_CATG'y
Cuts at: 1132 1229 2285 3350 4895 1132
Size: 97 1056 1065 1545 2229
Fragments arranged by size:
2229 1545 1065 1056 97
PasI CC'CwG_GG
Cuts at: 1348 2748 3035 1348
Size: 1400 287 4305
Fragments arranged by size:
4305 1400 287
PciI A'CATG_T
Cuts at: 4891 4891
Size: 5992
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2398 2487 2592 3247 1108
Size: 1290 89 105 655 3853
Fragments arranged by size:
3853 1290 655 105 89
PfoI T'CCnGG_A
Cuts at: 2932 2932
Size: 5992
PmeI GTTT'AAAC
Cuts at: 3296 3296
Size: 5992
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4061 4093 5574 5606 4061
Size: 32 1481 32 4447
Fragments arranged by size:
4447 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2234 2733 2234
Size: 499 5493
PshAI GACnn'nnGTC
Cuts at: 1613 2364 3096 3147 1613
Size: 751 732 51 4458
Fragments arranged by size:
4458 751 732 51
PsiI TTA'TAA
Cuts at: 3562 3562
Size: 5992
PspOMI G'GGCC_C
Cuts at: 2376 2753 2845 2376
Size: 377 92 5523
Fragments arranged by size:
5523 377 92
PstI C_TGCA'G
Cuts at: 830 2064 2242 3232 3344 830
Size: 1234 178 990 112 3478
Fragments arranged by size:
3478 1234 990 178 112
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5992
PvuII CAG'CTG
Cuts at: 2104 2592 2800 2104
Size: 488 208 5296
Fragments arranged by size:
5296 488 208
SacI G_AGCT'C
Cuts at: 2139 2139
Size: 5992
SalI G'TCGA_C
Cuts at: 1936 3334 1936
Size: 1398 4594
SanDI GG'GwC_CC
Cuts at: 2733 2733
Size: 5992
SapI GCTCTTCn'nnn_
Cuts at: 2441 2515 2441
Size: 74 5918
SbfI CC_TGCA'GG
Cuts at: 2064 3344 2064
Size: 1280 4712
ScaI AGT'ACT
Cuts at: 4240 5952 4240
Size: 1712 4280
SexAI A'CCwGG_T
Cuts at: 2198 2710 2198
Size: 512 5480
SfcI C'TryA_G
Cuts at: 826 1045 2060 2238 2344 3228 3340 4475 5156
Size: 219 1015 178 106 884 112 1135 681
Cuts at: 5156 5347 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1015 884 681 219 191 178
112 106
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5992
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5992
SmaI CCC'GGG
Cuts at: 1872 2028 3319 1872
Size: 156 1291 4545
Fragments arranged by size:
4545 1291 156
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2990 3138 4096 4997 5259 820
Size: 197 926 1047 148 958 901 262 1553
Fragments arranged by size:
1553 1047 958 926 901 262 197 148
SnaBI TAC'GTA
Cuts at: 493 3783 493
Size: 3290 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5992
SphI G_CATG'C
Cuts at: 1132 3350 1132
Size: 2218 3774
SspI AAT'ATT
Cuts at: 5 52 3916 5
Size: 47 3864 2081
Fragments arranged by size:
3864 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5992
StyI C'CwwG_G
Cuts at: 513 1065 2004 3431 513
Size: 552 939 1427 3074
Fragments arranged by size:
3074 1427 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2140 4019 4036 4189 4374 4819 325
Size: 1255 560 1879 17 153 185 445 1498
Fragments arranged by size:
1879 1498 1255 560 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2436 4238 5950
Size: 274 80 33 51 1443 459 1802 1712
Cuts at: 5950 96
Size: 138
Fragments arranged by size:
1802 1712 1443 459 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2679 2711 5952 5984 2679
Size: 32 3241 32 2687
Fragments arranged by size:
3241 2687 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5992
XbaI T'CTAG_A
Cuts at: 3328 3328
Size: 5992
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2203 2861 2203
Size: 658 5334
XhoI C'TCGA_G
Cuts at: 1943 2990 3138 1943
Size: 1047 148 4797
Fragments arranged by size:
4797 1047 148
XmaI C'CCGG_G
Cuts at: 1870 2026 3317 1870
Size: 156 1291 4545
Fragments arranged by size:
4545 1291 156
XmnI GAAnn'nnTTC
Cuts at: 4121 4121
Size: 5992
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3800 276
Size: 53 83 186 936 2266 2468
Fragments arranged by size:
2468 2266 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BstXI BstZ17I EcoNI EcoRV FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspXI PsrI RsrII
SacII SfiI SfoI SrfI SwaI