Restriction map for
pFN21AE5551
(Circular) (Six-base) MAPSORT of: pFN21AE5551.seq Check: 881 from: 1 to: 5938
pFN21AE5551 5938 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2065 2065
Size: 5938
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3748 278
Size: 53 83 186 936 2212 2468
Fragments arranged by size:
2468 2212 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2460 3145 3281 1937
Size: 523 685 136 4594
Fragments arranged by size:
4594 685 523 136
Acc65I G'GTAC_C
Cuts at: 3259 3259
Size: 5938
AclI AA'CG_TT
Cuts at: 1563 4065 4438 1563
Size: 2502 373 3063
Fragments arranged by size:
3063 2502 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4001 5385 5653 1632
Size: 2369 1384 268 1917
Fragments arranged by size:
2369 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5741
AflIII A'CryG_T
Cuts at: 1203 3114 4837 5814 1203
Size: 1911 1723 977 1327
Fragments arranged by size:
1911 1723 1327 977
AgeI A'CCGG_T
Cuts at: 4769 4769
Size: 5938
AhdI GACnn_n'nnGTC
Cuts at: 4667 4667
Size: 5938
AleI CACnn'nnGTG
Cuts at: 1166 2079 1166
Size: 913 5025
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5938
AlwNI CAG_nnn'CTG
Cuts at: 3681 5253 3681
Size: 1572 4366
ApaLI G'TGCA_C
Cuts at: 3995 5151 3995
Size: 1156 4782
ApoI r'AATT_y
Cuts at: 1493 2030 2084 2229 2759 3192 3206 3247 3473
Size: 537 54 145 530 433 14 41 226
Cuts at: 3473 3625 5734 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 537 530 433 226 152 145
54 41 14
AseI AT'TA_AT
Cuts at: 160 1032 3005 3009 4492 160
Size: 872 1973 4 1483 1606
Fragments arranged by size:
1973 1606 1483 872 4
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5938
AvaI C'yCGr_G
Cuts at: 1870 1943 3263 1870
Size: 73 1320 4545
Fragments arranged by size:
4545 1320 73
BamHI G'GATC_C
Cuts at: 3268 3635 3268
Size: 367 5571
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2053 3259 4714 618
Size: 325 217 627 266 1206 1455 1842
Fragments arranged by size:
1842 1455 1206 627 325 266 217
BanII G_rGCy'C
Cuts at: 1391 3257 1391
Size: 1866 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4967
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4129 4163 1903
Size: 34 2192 34 3678
Fragments arranged by size:
3678 2192 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2847 3830 5040 2847
Size: 983 1210 3745
Fragments arranged by size:
3745 1210 983
BclI T'GATC_A
Cuts at: 1548 2533 1548
Size: 985 4953
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4549 136
Size: 107 122 71 631 605 2877 1525
Fragments arranged by size:
2877 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2602 5933 2602
Size: 3331 2607
BlpI GC'TnA_GC
Cuts at: 3355 3355
Size: 5938
Bme1580I G_kGCm'C
Cuts at: 1790 2671 3999 5155 1790
Size: 881 1328 1156 2573
Fragments arranged by size:
2573 1328 1156 881
BmgBI CAC'GTC
Cuts at: 1318 3117 1318
Size: 1799 4139
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3669 4627 442
Size: 416 53 83 399 24 2252 958 1753
Fragments arranged by size:
2252 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5938
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4598 1239
Size: 3359 2579
Bpu10I CC'TnA_GC
Cuts at: 2493 2493
Size: 5938
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2583 4063 4928 5226 2583
Size: 1480 865 298 3295
Fragments arranged by size:
3295 1480 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4601 882
Size: 617 3102 2219
Fragments arranged by size:
3102 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3729 493
Size: 711 2525 2702
Fragments arranged by size:
2702 2525 711
BsaBI GATnn'nnATC
Cuts at: 5932 5932
Size: 5938
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3745 4127 4762 275
Size: 53 83 186 936 2212 382 635 1451
Fragments arranged by size:
2212 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4371 4769 5043 5190 5601 4371
Size: 398 274 147 411 4708
Fragments arranged by size:
4708 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5713
Fragments arranged by size:
5713 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2063 1186
Size: 877 5061
BseYI C'CCAG_C
Cuts at: 5141 5637 5141
Size: 496 5442
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3071 1163
Size: 573 1335 4030
Fragments arranged by size:
4030 1335 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3388 4149 5177 1540
Size: 275 186 1387 761 1028 2301
Fragments arranged by size:
2301 1387 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3999 4084 5155 3999
Size: 85 1071 4782
Fragments arranged by size:
4782 1071 85
BsmI GAATG_Cn'
Cuts at: 3449 3542 3449
Size: 93 5845
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2671 3257 3999 4084 5155 1391
Size: 399 881 586 742 85 1071 2174
Fragments arranged by size:
2174 1071 881 742 586 399 85
BspEI T'CCGG_A
Cuts at: 5601 5601
Size: 5938
BspHI T'CATG_A
Cuts at: 2523 3827 5557 2523
Size: 1304 1730 2904
Fragments arranged by size:
2904 1730 1304
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2065 3293 844
Size: 1221 1228 3489
Fragments arranged by size:
3489 1228 1221
BsrBI CCG'CTC
Cuts at: 1394 1823 3825 1394
Size: 429 2002 3507
Fragments arranged by size:
3507 2002 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4433 4607 65
Size: 1183 3185 174 1396
Fragments arranged by size:
3185 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4582 4769 1700
Size: 110 2772 187 2869
Fragments arranged by size:
2869 2772 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5938
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5938
BssSI C'ACGA_G
Cuts at: 3998 5010 3998
Size: 1012 4926
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5938
BstBI TT'CG_AA
Cuts at: 3065 4742 3065
Size: 1677 4261
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5938
BstYI r'GATC_y
Cuts at: 1972 2602 3268 3635 4020 4037 5489 5575 5587
Size: 630 666 367 385 17 1452 86 12
Cuts at: 5587 5933 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 666 630 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5386
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5199
Fragments arranged by size:
5199 683 56
BtsI GCAGTG_nn'
Cuts at: 2479 3454 4248 4268 2479
Size: 975 794 20 4149
Fragments arranged by size:
4149 975 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5938
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5903
DraI TTT'AAA
Cuts at: 2035 2089 3242 2035
Size: 54 1153 4731
Fragments arranged by size:
4731 1153 54
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4945 809
Size: 507 3629 1802
Fragments arranged by size:
3629 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3385 4274 5903
Size: 54 1425 50 275 18 1555 889 1629
Cuts at: 5903 8
Size: 43
Fragments arranged by size:
1629 1555 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3385 1537
Size: 275 1573 4090
Fragments arranged by size:
4090 1573 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3067 3868 1353
Size: 1714 801 3423
Fragments arranged by size:
3423 1714 801
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4525 4899 5045 387
Size: 1064 138 2936 374 146 1280
Fragments arranged by size:
2936 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4001 4598 5385 5653 1239
Size: 393 2369 597 787 268 1524
Fragments arranged by size:
2369 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3247 3625 3247
Size: 378 5560
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2406 2959 2406
Size: 553 5385
FspI TGC'GCA
Cuts at: 4444 4444
Size: 5938
HaeII r_GCGC'y
Cuts at: 1678 5085 1678
Size: 3407 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2518 2550 2841 2873 4579
Size: 32 135 32 946 32 291 32 1706
Cuts at: 4579 4611 4653 4685 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1706 946 291 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3282 3528 669
Size: 988 281 1344 246 3079
Fragments arranged by size:
3079 1344 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5938
HpaI GTT'AAC
Cuts at: 3528 3528
Size: 5938
KpnI G_GTAC'C
Cuts at: 3263 3263
Size: 5938
MfeI C'AATT_G
Cuts at: 2406 3088 3537 2406
Size: 682 449 4807
Fragments arranged by size:
4807 682 449
MluI A'CGCG_T
Cuts at: 5814 5814
Size: 5938
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2779 5052 5236 1305
Size: 1474 2273 184 2007
Fragments arranged by size:
2273 2007 1474 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4116
Fragments arranged by size:
4116 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2079 2182 3896 4255 4414 518
Size: 648 913 103 1714 359 159 2042
Fragments arranged by size:
2042 1714 913 648 359 159 103
MspA1I CmG'CkG
Cuts at: 1606 3354 4031 5179 5424 1606
Size: 1748 677 1148 245 2120
Fragments arranged by size:
2120 1748 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5386
NdeI CA'TA_TG
Cuts at: 387 2179 387
Size: 1792 4146
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5938
NotI GC'GGCC_GC
Cuts at: 3385 3385
Size: 5938
NspI r_CATG'y
Cuts at: 1132 1229 3296 4841 1132
Size: 97 2067 1545 2229
Fragments arranged by size:
2229 2067 1545 97
PacI TTA_AT'TAA
Cuts at: 3009 3009
Size: 5938
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5938
PciI A'CATG_T
Cuts at: 4837 4837
Size: 5938
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2378 1108
Size: 1270 4668
PmeI GTTT'AAAC
Cuts at: 3242 3242
Size: 5938
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4007 4039 5520 5552 4007
Size: 32 1481 32 4393
Fragments arranged by size:
4393 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3090 1613
Size: 1477 4461
PsiI TTA'TAA
Cuts at: 2864 3508 2864
Size: 644 5294
PstI C_TGCA'G
Cuts at: 830 3290 830
Size: 2460 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5938
SalI G'TCGA_C
Cuts at: 1936 3280 1936
Size: 1344 4594
SbfI CC_TGCA'GG
Cuts at: 3290 3290
Size: 5938
ScaI AGT'ACT
Cuts at: 2953 4186 5898 2953
Size: 1233 1712 2993
Fragments arranged by size:
2993 1712 1233
SfcI C'TryA_G
Cuts at: 826 1045 2395 3286 4421 5102 5293 826
Size: 219 1350 891 1135 681 191 1471
Fragments arranged by size:
1471 1350 1135 891 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5938
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5938
SmaI CCC'GGG
Cuts at: 1872 3265 1872
Size: 1393 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2598 4042 4943 5205 820
Size: 197 926 655 1444 901 262 1553
Fragments arranged by size:
1553 1444 926 901 655 262 197
SnaBI TAC'GTA
Cuts at: 493 3729 493
Size: 3236 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5938
SphI G_CATG'C
Cuts at: 1132 3296 1132
Size: 2164 3774
SspI AAT'ATT
Cuts at: 5 52 2691 3862 5
Size: 47 2639 1171 2081
Fragments arranged by size:
2639 2081 1171 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5938
StyI C'CwwG_G
Cuts at: 513 1065 2574 3377 513
Size: 552 1509 803 3074
Fragments arranged by size:
3074 1509 803 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3965 3982 4135 4320 4765 325
Size: 1255 2385 17 153 185 445 1498
Fragments arranged by size:
2385 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2951 4184 5896
Size: 274 80 33 51 1443 974 1233 1712
Cuts at: 5896 96
Size: 138
Fragments arranged by size:
1712 1443 1233 974 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5898 5930 5898
Size: 32 5906
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5938
XbaI T'CTAG_A
Cuts at: 2428 3274 2428
Size: 846 5092
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3102 3102
Size: 5938
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5938
XmaI C'CCGG_G
Cuts at: 1870 3263 1870
Size: 1393 4545
XmnI GAAnn'nnTTC
Cuts at: 2968 4067 2968
Size: 1099 4839
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3746 276
Size: 53 83 186 936 2212 2468
Fragments arranged by size:
2468 2212 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PacI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI