Restriction map for
pFN21AE5546
(Circular) (Six-base) MAPSORT of: pFN21AE5546.seq Check: 7436 from: 1 to: 5872
pFN21AE5546 5872 bp
With 182 enzymes: SgfI *
December 8, 2014 13:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3682 278
Size: 53 83 186 936 2146 2468
Fragments arranged by size:
2468 2146 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3215 1937
Size: 1278 4594
Acc65I G'GTAC_C
Cuts at: 3193 3193
Size: 5872
AclI AA'CG_TT
Cuts at: 1563 3999 4372 1563
Size: 2436 373 3063
Fragments arranged by size:
3063 2436 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3935 5319 5587 1632
Size: 2303 1384 268 1917
Fragments arranged by size:
2303 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5675
AflIII A'CryG_T
Cuts at: 1203 2844 4771 5748 1203
Size: 1641 1927 977 1327
Fragments arranged by size:
1927 1641 1327 977
AgeI A'CCGG_T
Cuts at: 4703 4703
Size: 5872
AhdI GACnn_n'nnGTC
Cuts at: 4601 4601
Size: 5872
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5872
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5872
AlwNI CAG_nnn'CTG
Cuts at: 2584 3148 3615 5187 2584
Size: 564 467 1572 3269
Fragments arranged by size:
3269 1572 564 467
ApaI G_GGCC'C
Cuts at: 2054 2208 2054
Size: 154 5718
ApaLI G'TGCA_C
Cuts at: 2171 2711 3929 5085 2171
Size: 540 1218 1156 2958
Fragments arranged by size:
2958 1218 1156 540
ApoI r'AATT_y
Cuts at: 1493 3181 3407 3559 5668 1493
Size: 1688 226 152 2109 1697
Fragments arranged by size:
2109 1697 1688 226 152
AscI GG'CGCG_CC
Cuts at: 2065 2065
Size: 5872
AseI AT'TA_AT
Cuts at: 160 1032 4426 160
Size: 872 3394 1606
Fragments arranged by size:
3394 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5872
AvaI C'yCGr_G
Cuts at: 1870 1943 2053 3197 1870
Size: 73 110 1144 4545
Fragments arranged by size:
4545 1144 110 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2556 2589 2556
Size: 33 5839
BamHI G'GATC_C
Cuts at: 3202 3569 3202
Size: 367 5505
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2272 2663 3193 4648 618
Size: 325 217 627 485 391 530 1455 1842
Fragments arranged by size:
1842 1455 627 530 485 391 325 217
BanII G_rGCy'C
Cuts at: 1391 2054 2208 2631 2869 2892 2979 3191 1391
Size: 663 154 423 238 23 87 212 4072
Fragments arranged by size:
4072 663 423 238 212 154 87 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4901
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4063 4097 1903
Size: 34 2126 34 3678
Fragments arranged by size:
3678 2126 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2584 3764 4974 2584
Size: 1180 1210 3482
Fragments arranged by size:
3482 1210 1180
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5872
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2058 4483 136
Size: 107 122 71 631 605 386 2425 1525
Fragments arranged by size:
2425 1525 631 605 386 122 107 71
BglII A'GATC_T
Cuts at: 5867 5867
Size: 5872
BlpI GC'TnA_GC
Cuts at: 3289 3289
Size: 5872
Bme1580I G_kGCm'C
Cuts at: 1790 2054 2175 2208 2546 2715 3933 5089 1790
Size: 264 121 33 338 169 1218 1156 2573
Fragments arranged by size:
2573 1218 1156 338 264 169 121 33
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5872
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2572 3603 4561
Size: 416 53 83 399 24 1155 1031 958
Cuts at: 4561 442
Size: 1753
Fragments arranged by size:
1753 1155 1031 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5872
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2521 2521
Size: 5872
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2723 3137 4532 1239
Size: 1484 414 1395 2579
Fragments arranged by size:
2579 1484 1395 414
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3997 4862 5160 3997
Size: 865 298 4709
Fragments arranged by size:
4709 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2297 4535 882
Size: 617 798 2238 2219
Fragments arranged by size:
2238 2219 798 617
BsaAI yAC'GTr
Cuts at: 493 1204 2845 3663 493
Size: 711 1641 818 2702
Fragments arranged by size:
2702 1641 818 711
BsaBI GATnn'nnATC
Cuts at: 5866 5866
Size: 5872
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3679 4061 4696 275
Size: 53 83 186 936 2146 382 635 1451
Fragments arranged by size:
2146 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2010 4305 4703 4977 5124 5535 2010
Size: 2295 398 274 147 411 2347
Fragments arranged by size:
2347 2295 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2102 2132 1178
Size: 30 165 30 699 30 4918
Fragments arranged by size:
4918 699 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2198 2240 1186
Size: 1012 42 4818
Fragments arranged by size:
4818 1012 42
BseYI C'CCAG_C
Cuts at: 2399 2631 5075 5571 2399
Size: 232 2444 496 2700
Fragments arranged by size:
2700 2444 496 232
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2264 1163
Size: 573 528 4771
Fragments arranged by size:
4771 573 528
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3322 4083 5111 1540
Size: 275 186 1321 761 1028 2301
Fragments arranged by size:
2301 1321 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2175 2715 2892 3933 4018 5089 2175
Size: 540 177 1041 85 1071 2958
Fragments arranged by size:
2958 1071 1041 540 177 85
BsiWI C'GTAC_G
Cuts at: 2871 2871
Size: 5872
BsmI GAATG_Cn'
Cuts at: 2420 3383 3476 2420
Size: 963 93 4816
Fragments arranged by size:
4816 963 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2175 2208 2546 2631 2715 2869
Size: 399 264 121 33 338 85 84 154
Cuts at: 2869 2892 2979 3191 3933 4018 5089 1391
Size: 23 87 212 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 338 264 212 154
121 87 85 85 84 33 23
BspEI T'CCGG_A
Cuts at: 2010 5535 2010
Size: 3525 2347
BspHI T'CATG_A
Cuts at: 2799 3761 5491 2799
Size: 962 1730 3180
Fragments arranged by size:
3180 1730 962
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3227 844
Size: 2383 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3759 1394
Size: 429 1936 3507
Fragments arranged by size:
3507 1936 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2350 4367 4541 65
Size: 1183 1102 2017 174 1396
Fragments arranged by size:
2017 1396 1183 1102 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2838 4516 4703 1700
Size: 110 1028 1678 187 2869
Fragments arranged by size:
2869 1678 1028 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5872
BssHII G'CGCG_C
Cuts at: 1925 2065 1925
Size: 140 5732
BssSI C'ACGA_G
Cuts at: 2450 3932 4944 2450
Size: 1482 1012 3378
Fragments arranged by size:
3378 1482 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5872
BstBI TT'CG_AA
Cuts at: 4676 4676
Size: 5872
BstEII G'GTnAC_C
Cuts at: 1184 2470 1184
Size: 1286 4586
BstXI CCAn_nnnn'nTGG
Cuts at: 3064 3106 3064
Size: 42 5830
BstYI r'GATC_y
Cuts at: 1972 3202 3569 3954 3971 5423 5509 5521 5867
Size: 1230 367 385 17 1452 86 12 346
Cuts at: 5867 1972
Size: 1977
Fragments arranged by size:
1977 1452 1230 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2145 513
Size: 552 1080 4240
Fragments arranged by size:
4240 1080 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2100 2725 3174 487
Size: 683 56 874 625 449 3185
Fragments arranged by size:
3185 874 683 625 449 56
BtsI GCAGTG_nn'
Cuts at: 3388 4182 4202 3388
Size: 794 20 5058
Fragments arranged by size:
5058 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5872
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5837
DraI TTT'AAA
Cuts at: 3176 3176
Size: 5872
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2765 3038 4879 809
Size: 507 1449 273 1841 1802
Fragments arranged by size:
1841 1802 1449 507 273
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2113 2368 3319
Size: 54 1425 50 275 18 283 255 951
Cuts at: 3319 4208 5837 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 951 889 283 275 255 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3319 1537
Size: 275 1507 4090
Fragments arranged by size:
4090 1507 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3802 1353
Size: 2449 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2569 4459 4833 4979 387
Size: 1064 138 980 1890 374 146 1280
Fragments arranged by size:
1890 1280 1064 980 374 146 138
EcoICRI GAG'CTC
Cuts at: 2890 2890
Size: 5872
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2723 3137 3935 4532 5319 5587 1239
Size: 393 1091 414 798 597 787 268 1524
Fragments arranged by size:
1524 1091 798 787 597 414 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2705 2705
Size: 5872
EcoO109I rG'GnC_Cy
Cuts at: 2204 2205 2730 2204
Size: 1 525 5346
Fragments arranged by size:
5346 525 1
EcoRI G'AATT_C
Cuts at: 3181 3559 3181
Size: 378 5494
FspI TGC'GCA
Cuts at: 4378 4378
Size: 5872
HaeII r_GCGC'y
Cuts at: 1678 5019 1678
Size: 3341 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4513 4545 4587 4619 1373
Size: 32 135 32 2941 32 42 32 2626
Fragments arranged by size:
2941 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3216 3462 669
Size: 988 281 1278 246 3079
Fragments arranged by size:
3079 1278 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5872
HpaI GTT'AAC
Cuts at: 3462 3462
Size: 5872
KpnI G_GTAC'C
Cuts at: 3197 3197
Size: 5872
MfeI C'AATT_G
Cuts at: 3471 3471
Size: 5872
MluI A'CGCG_T
Cuts at: 5748 5748
Size: 5872
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2145 2985 3045 4986 5170 1305
Size: 840 840 60 1941 184 2007
Fragments arranged by size:
2007 1941 840 840 184 60
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4050
Fragments arranged by size:
4050 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2178 2343 2654 3830 4189 4348 518
Size: 648 1012 165 311 1176 359 159 2042
Fragments arranged by size:
2042 1176 1012 648 359 311 165 159
MspA1I CmG'CkG
Cuts at: 1606 2128 2279 3046 3288 3965 5113 5358 1606
Size: 522 151 767 242 677 1148 245 2120
Fragments arranged by size:
2120 1148 767 677 522 245 242 151
NcoI C'CATG_G
Cuts at: 513 1065 2145 513
Size: 552 1080 4240
Fragments arranged by size:
4240 1080 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5872
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5872
NotI GC'GGCC_GC
Cuts at: 3319 3319
Size: 5872
NspI r_CATG'y
Cuts at: 1132 1229 2464 2693 3230 4775 1132
Size: 97 1235 229 537 1545 2229
Fragments arranged by size:
2229 1545 1235 537 229 97
PasI CC'CwG_GG
Cuts at: 1348 3148 1348
Size: 1800 4072
PciI A'CATG_T
Cuts at: 4771 4771
Size: 5872
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2256 1108
Size: 1148 4724
PfoI T'CCnGG_A
Cuts at: 2700 3114 2700
Size: 414 5458
PmeI GTTT'AAAC
Cuts at: 3176 3176
Size: 5872
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3941 3973 5454 5486 3941
Size: 32 1481 32 4327
Fragments arranged by size:
4327 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5872
PsiI TTA'TAA
Cuts at: 3442 3442
Size: 5872
PspOMI G'GGCC_C
Cuts at: 2050 2204 2050
Size: 154 5718
PstI C_TGCA'G
Cuts at: 830 2032 2589 3224 830
Size: 1202 557 635 3478
Fragments arranged by size:
3478 1202 635 557
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5872
PvuII CAG'CTG
Cuts at: 2128 2128
Size: 5872
SacI G_AGCT'C
Cuts at: 2892 2892
Size: 5872
SalI G'TCGA_C
Cuts at: 1936 3214 1936
Size: 1278 4594
SbfI CC_TGCA'GG
Cuts at: 3224 3224
Size: 5872
ScaI AGT'ACT
Cuts at: 4120 5832 4120
Size: 1712 4160
SexAI A'CCwGG_T
Cuts at: 2913 2913
Size: 5872
SfcI C'TryA_G
Cuts at: 826 1045 2028 2361 2585 3220 4355 5036 5227
Size: 219 983 333 224 635 1135 681 191
Cuts at: 5227 826
Size: 1471
Fragments arranged by size:
1471 1135 983 681 635 333 224 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5872
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5872
SmaI CCC'GGG
Cuts at: 1872 2055 3199 1872
Size: 183 1144 4545
Fragments arranged by size:
4545 1144 183
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3976 4877 5139 820
Size: 197 926 2033 901 262 1553
Fragments arranged by size:
2033 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3663 493
Size: 3170 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5872
SphI G_CATG'C
Cuts at: 1132 2464 3230 1132
Size: 1332 766 3774
Fragments arranged by size:
3774 1332 766
SspI AAT'ATT
Cuts at: 5 52 3796 5
Size: 47 3744 2081
Fragments arranged by size:
3744 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5872
StyI C'CwwG_G
Cuts at: 513 1065 2145 3099 3311 513
Size: 552 1080 954 212 3074
Fragments arranged by size:
3074 1080 954 552 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2138 2248 3899 3916 4069 4254 4699
Size: 1255 558 110 1651 17 153 185 445
Cuts at: 4699 325
Size: 1498
Fragments arranged by size:
1651 1498 1255 558 445 185 153 110
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4118 5830 96
Size: 274 80 33 51 1443 2141 1712 138
Fragments arranged by size:
2141 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2903 2935 5832 5864 2903
Size: 32 2897 32 2911
Fragments arranged by size:
2911 2897 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5872
XbaI T'CTAG_A
Cuts at: 3208 3208
Size: 5872
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2257 2449 2615 2257
Size: 192 166 5514
Fragments arranged by size:
5514 192 166
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5872
XmaI C'CCGG_G
Cuts at: 1870 2053 3197 1870
Size: 183 1144 4545
Fragments arranged by size:
4545 1144 183
XmnI GAAnn'nnTTC
Cuts at: 4001 4001
Size: 5872
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3680 276
Size: 53 83 186 936 2146 2468
Fragments arranged by size:
2468 2146 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AvrII BbeI BbvCI BfrBI Bpu10I
BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspXI PsrI RsrII SacII SanDI SapI SfiI
SfoI SrfI SwaI