Restriction map for
pFN21AE5545
(Circular) (Six-base) MAPSORT of: pFN21AE5545.seq Check: 3892 from: 1 to: 5869
pFN21AE5545 5869 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2412 2412
Size: 5869
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3679 278
Size: 53 83 186 936 2143 2468
Fragments arranged by size:
2468 2143 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3212 1937
Size: 1275 4594
Acc65I G'GTAC_C
Cuts at: 3190 3190
Size: 5869
AclI AA'CG_TT
Cuts at: 1563 3996 4369 1563
Size: 2433 373 3063
Fragments arranged by size:
3063 2433 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3932 5316 5584 1632
Size: 2300 1384 268 1917
Fragments arranged by size:
2300 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5672
AflIII A'CryG_T
Cuts at: 1203 2331 4768 5745 1203
Size: 1128 2437 977 1327
Fragments arranged by size:
2437 1327 1128 977
AgeI A'CCGG_T
Cuts at: 4700 4700
Size: 5869
AhdI GACnn_n'nnGTC
Cuts at: 4598 4598
Size: 5869
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5869
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5869
AlwNI CAG_nnn'CTG
Cuts at: 2015 2602 2626 3004 3612 5184 2015
Size: 587 24 378 608 1572 2700
Fragments arranged by size:
2700 1572 608 587 378 24
ApaI G_GGCC'C
Cuts at: 2063 2063
Size: 5869
ApaLI G'TGCA_C
Cuts at: 3926 5082 3926
Size: 1156 4713
ApoI r'AATT_y
Cuts at: 1493 3178 3404 3556 5665 1493
Size: 1685 226 152 2109 1697
Fragments arranged by size:
2109 1697 1685 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4423 160
Size: 872 3391 1606
Fragments arranged by size:
3391 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5869
AvaI C'yCGr_G
Cuts at: 1870 1943 2049 2135 2411 2552 2910 2989 3137
Size: 73 106 86 276 141 358 79 148
Cuts at: 3137 3194 1870
Size: 57 4545
Fragments arranged by size:
4545 358 276 148 141 106 86 79
73 57
BamHI G'GATC_C
Cuts at: 3199 3566 3199
Size: 367 5502
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3046 3190 4645 618
Size: 325 217 627 1259 144 1455 1842
Fragments arranged by size:
1842 1455 1259 627 325 217 144
BanII G_rGCy'C
Cuts at: 1391 2063 2135 2169 2640 3144 3188 1391
Size: 672 72 34 471 504 44 4072
Fragments arranged by size:
4072 672 504 471 72 44 34
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2665 928
Size: 971 766 4132
Fragments arranged by size:
4132 971 766
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4060 4094 1903
Size: 34 2123 34 3678
Fragments arranged by size:
3678 2123 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3761 4971 3761
Size: 1210 4659
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5869
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2192 3110 4480
Size: 107 122 71 631 605 520 918 1370
Cuts at: 4480 136
Size: 1525
Fragments arranged by size:
1525 1370 918 631 605 520 122 107
71
BglII A'GATC_T
Cuts at: 2325 5864 2325
Size: 3539 2330
BlpI GC'TnA_GC
Cuts at: 2570 2786 3286 2570
Size: 216 500 5153
Fragments arranged by size:
5153 500 216
Bme1580I G_kGCm'C
Cuts at: 1790 2063 2900 3051 3930 5086 1790
Size: 273 837 151 879 1156 2573
Fragments arranged by size:
2573 1156 879 837 273 151
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5869
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2238 2297 3600
Size: 416 53 83 399 24 821 59 1303
Cuts at: 3600 4558 442
Size: 958 1753
Fragments arranged by size:
1753 1303 958 821 416 399 83 59
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5869
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2197 3139 2197
Size: 942 4927
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2054 2624 3110 4529 1239
Size: 815 570 486 1419 2579
Fragments arranged by size:
2579 1419 815 570 486
Bpu10I CC'TnA_GC
Cuts at: 2762 2762
Size: 5869
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3994 4859 5157 3994
Size: 865 298 4706
Fragments arranged by size:
4706 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2570 4532 882
Size: 617 1071 1962 2219
Fragments arranged by size:
2219 1962 1071 617
BsaAI yAC'GTr
Cuts at: 493 1204 2566 3660 493
Size: 711 1362 1094 2702
Fragments arranged by size:
2702 1362 1094 711
BsaBI GATnn'nnATC
Cuts at: 5863 5863
Size: 5869
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2259 3676 4058 4693
Size: 53 83 186 936 726 1417 382 635
Cuts at: 4693 275
Size: 1451
Fragments arranged by size:
1451 1417 936 726 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2427 4302 4700 4974 5121 5532 2427
Size: 1875 398 274 147 411 2764
Fragments arranged by size:
2764 1875 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2408 2438 3126 3156 1178
Size: 30 165 30 1005 30 688 30 3891
Fragments arranged by size:
3891 1005 688 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2429 2432 2570 2753 2837 1186
Size: 1243 3 138 183 84 4218
Fragments arranged by size:
4218 1243 183 138 84 3
BseYI C'CCAG_C
Cuts at: 2362 2589 2920 5072 5568 2362
Size: 227 331 2152 496 2663
Fragments arranged by size:
2663 2152 496 331 227
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2390 1163
Size: 573 654 4642
Fragments arranged by size:
4642 654 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3319 4080 5108 1540
Size: 275 186 1318 761 1028 2301
Fragments arranged by size:
2301 1318 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3144 3930 4015 5086 3144
Size: 786 85 1071 3927
Fragments arranged by size:
3927 1071 786 85
BsmI GAATG_Cn'
Cuts at: 3380 3473 3380
Size: 93 5776
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2063 2135 2169 2640 2900 3051 3144
Size: 399 273 72 34 471 260 151 93
Cuts at: 3144 3188 3930 4015 5086 1391
Size: 44 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 471 399 273 260 151
93 85 72 44 34
BspEI T'CCGG_A
Cuts at: 5532 5532
Size: 5869
BspHI T'CATG_A
Cuts at: 2319 3758 5488 2319
Size: 1439 1730 2700
Fragments arranged by size:
2700 1730 1439
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2412 3224 844
Size: 1568 812 3489
Fragments arranged by size:
3489 1568 812
BsrBI CCG'CTC
Cuts at: 1394 1823 2224 2782 2917 3756 1394
Size: 429 401 558 135 839 3507
Fragments arranged by size:
3507 839 558 429 401 135
BsrDI GCAATG_nn'
Cuts at: 65 1248 2376 4364 4538 65
Size: 1183 1128 1988 174 1396
Fragments arranged by size:
1988 1396 1183 1128 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4513 4700 1700
Size: 110 2703 187 2869
Fragments arranged by size:
2869 2703 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5869
BssHII G'CGCG_C
Cuts at: 1925 2644 1925
Size: 719 5150
BssSI C'ACGA_G
Cuts at: 2489 3070 3929 4941 2489
Size: 581 859 1012 3417
Fragments arranged by size:
3417 1012 859 581
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2015 2472 794
Size: 1221 457 4191
Fragments arranged by size:
4191 1221 457
BstBI TT'CG_AA
Cuts at: 2703 4673 2703
Size: 1970 3899
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5869
BstXI CCAn_nnnn'nTGG
Cuts at: 2389 2389
Size: 5869
BstYI r'GATC_y
Cuts at: 1972 2325 3199 3566 3951 3968 5420 5506 5518
Size: 353 874 367 385 17 1452 86 12
Cuts at: 5518 5864 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 874 385 367 353 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2240 2240
Size: 5869
BtgI C'CryG_G
Cuts at: 513 1065 2004 3105 513
Size: 552 939 1101 3277
Fragments arranged by size:
3277 1101 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2052 487
Size: 683 56 826 4304
Fragments arranged by size:
4304 826 683 56
BtsI GCAGTG_nn'
Cuts at: 3385 4179 4199 3385
Size: 794 20 5055
Fragments arranged by size:
5055 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5869
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5834
DraI TTT'AAA
Cuts at: 3173 3173
Size: 5869
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4876 809
Size: 507 3560 1802
Fragments arranged by size:
3560 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2307 3316 4205
Size: 54 1425 50 275 18 477 1009 889
Cuts at: 4205 5834 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1009 889 477 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3316 1537
Size: 275 1504 4090
Fragments arranged by size:
4090 1504 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3799 1353
Size: 2446 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4456 4830 4976 387
Size: 1064 138 2867 374 146 1280
Fragments arranged by size:
2867 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3142 3142
Size: 5869
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2624 3110 3932 4529 5316 5584
Size: 393 422 570 486 822 597 787 268
Cuts at: 5584 1239
Size: 1524
Fragments arranged by size:
1524 822 787 597 570 486 422 393
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2243 2891 2243
Size: 648 5221
EcoO109I rG'GnC_Cy
Cuts at: 2059 2060 2140 2484 2548 2950 2059
Size: 1 80 344 64 402 4978
Fragments arranged by size:
4978 402 344 80 64 1
EcoRI G'AATT_C
Cuts at: 3178 3556 3178
Size: 378 5491
FspI TGC'GCA
Cuts at: 2840 4375 2840
Size: 1535 4334
HaeII r_GCGC'y
Cuts at: 1678 2668 5016 1678
Size: 990 2348 2531
Fragments arranged by size:
2531 2348 990
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3131 3163 4510 4542 4584
Size: 32 135 32 1559 32 1347 32 42
Cuts at: 4584 4616 1373
Size: 32 2626
Fragments arranged by size:
2626 1559 1347 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3213 3459 669
Size: 988 281 1275 246 3079
Fragments arranged by size:
3079 1275 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5869
HpaI GTT'AAC
Cuts at: 3459 3459
Size: 5869
KpnI G_GTAC'C
Cuts at: 3194 3194
Size: 5869
MfeI C'AATT_G
Cuts at: 3468 3468
Size: 5869
MluI A'CGCG_T
Cuts at: 5745 5745
Size: 5869
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4983 5167 1305
Size: 3678 184 2007
Fragments arranged by size:
3678 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4047
Fragments arranged by size:
4047 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3083 3827 4186 4345 518
Size: 648 1917 744 359 159 2042
Fragments arranged by size:
2042 1917 744 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2015 2127 2254 2623 2830 3107 3285 3962
Size: 409 112 127 369 207 277 178 677
Cuts at: 3962 5110 5355 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 409 369 277 245 207
178 127 112
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4378
Fragments arranged by size:
4378 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5869
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5869
NotI GC'GGCC_GC
Cuts at: 3316 3316
Size: 5869
NspI r_CATG'y
Cuts at: 1132 1229 3227 4772 1132
Size: 97 1998 1545 2229
Fragments arranged by size:
2229 1998 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2144 1348
Size: 796 5073
PciI A'CATG_T
Cuts at: 4768 4768
Size: 5869
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2602 1108
Size: 1494 4375
PfoI T'CCnGG_A
Cuts at: 2943 2943
Size: 5869
PmeI GTTT'AAAC
Cuts at: 3173 3173
Size: 5869
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3938 3970 5451 5483 3938
Size: 32 1481 32 4324
Fragments arranged by size:
4324 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2484 2548 2484
Size: 64 5805
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5869
PsiI TTA'TAA
Cuts at: 3439 3439
Size: 5869
PspOMI G'GGCC_C
Cuts at: 2059 2059
Size: 5869
PstI C_TGCA'G
Cuts at: 830 2445 2895 2958 3221 830
Size: 1615 450 63 263 3478
Fragments arranged by size:
3478 1615 450 263 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5869
PvuII CAG'CTG
Cuts at: 2623 2623
Size: 5869
SacI G_AGCT'C
Cuts at: 3144 3144
Size: 5869
SacII CC_GC'GG
Cuts at: 3108 3108
Size: 5869
SalI G'TCGA_C
Cuts at: 1936 3211 1936
Size: 1275 4594
SanDI GG'GwC_CC
Cuts at: 2484 2484
Size: 5869
SbfI CC_TGCA'GG
Cuts at: 2895 3221 2895
Size: 326 5543
ScaI AGT'ACT
Cuts at: 4117 5829 4117
Size: 1712 4157
SexAI A'CCwGG_T
Cuts at: 2088 2088
Size: 5869
SfcI C'TryA_G
Cuts at: 826 1045 2441 2891 2954 3217 4352 5033 5224
Size: 219 1396 450 63 263 1135 681 191
Cuts at: 5224 826
Size: 1471
Fragments arranged by size:
1471 1396 1135 681 450 263 219 191
63
SfiI GGCCn_nnn'nGGCC
Cuts at: 3110 3110
Size: 5869
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5869
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5869
SmaI CCC'GGG
Cuts at: 1872 2051 3196 1872
Size: 179 1145 4545
Fragments arranged by size:
4545 1145 179
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3973 4874 5136 820
Size: 197 926 2030 901 262 1553
Fragments arranged by size:
2030 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3660 493
Size: 3167 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5869
SphI G_CATG'C
Cuts at: 1132 3227 1132
Size: 2095 3774
SspI AAT'ATT
Cuts at: 5 52 3793 5
Size: 47 3741 2081
Fragments arranged by size:
3741 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5869
StyI C'CwwG_G
Cuts at: 513 1065 2004 2583 2802 3308 513
Size: 552 939 579 219 506 3074
Fragments arranged by size:
3074 939 579 552 506 219
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2354 3896 3913 4066 4251 4696 325
Size: 1255 774 1542 17 153 185 445 1498
Fragments arranged by size:
1542 1498 1255 774 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2208 2844 4115
Size: 274 80 33 51 1443 231 636 1271
Cuts at: 4115 5827 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1271 636 274 231 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5829 5861 5829
Size: 32 5837
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5869
XbaI T'CTAG_A
Cuts at: 3205 3205
Size: 5869
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2302 2302
Size: 5869
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5869
XmaI C'CCGG_G
Cuts at: 1870 2049 3194 1870
Size: 179 1145 4545
Fragments arranged by size:
4545 1145 179
XmnI GAAnn'nnTTC
Cuts at: 3998 3998
Size: 5869
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3677 276
Size: 53 83 186 936 2143 2468
Fragments arranged by size:
2468 2143 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SanDI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI RsrII SapI SfoI SrfI SwaI