Restriction map for
pFN21AE5544
(Circular) (Six-base) MAPSORT of: pFN21AE5544.seq Check: 3726 from: 1 to: 5848
pFN21AE5544 5848 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2697 2697
Size: 5848
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3658 278
Size: 53 83 186 936 2122 2468
Fragments arranged by size:
2468 2122 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3048 3191 1937
Size: 1111 143 4594
Fragments arranged by size:
4594 1111 143
Acc65I G'GTAC_C
Cuts at: 3169 3169
Size: 5848
AclI AA'CG_TT
Cuts at: 1563 3975 4348 1563
Size: 2412 373 3063
Fragments arranged by size:
3063 2412 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3911 5295 5563 1632
Size: 2279 1384 268 1917
Fragments arranged by size:
2279 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 3059 820
Size: 197 2042 3609
Fragments arranged by size:
3609 2042 197
AflIII A'CryG_T
Cuts at: 1203 2336 4747 5724 1203
Size: 1133 2411 977 1327
Fragments arranged by size:
2411 1327 1133 977
AgeI A'CCGG_T
Cuts at: 4679 4679
Size: 5848
AhdI GACnn_n'nnGTC
Cuts at: 4577 4577
Size: 5848
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5848
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5848
AlwNI CAG_nnn'CTG
Cuts at: 2101 2453 2698 2779 3591 5163 2101
Size: 352 245 81 812 1572 2786
Fragments arranged by size:
2786 1572 812 352 245 81
ApaI G_GGCC'C
Cuts at: 2037 2578 2037
Size: 541 5307
ApaLI G'TGCA_C
Cuts at: 3905 5061 3905
Size: 1156 4692
ApoI r'AATT_y
Cuts at: 1493 2922 3157 3383 3535 5644 1493
Size: 1429 235 226 152 2109 1697
Fragments arranged by size:
2109 1697 1429 235 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4402 160
Size: 872 3370 1606
Fragments arranged by size:
3370 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5848
AvaI C'yCGr_G
Cuts at: 1870 1943 2036 3090 3173 1870
Size: 73 93 1054 83 4545
Fragments arranged by size:
4545 1054 93 83 73
BamHI G'GATC_C
Cuts at: 3178 3545 3178
Size: 367 5481
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3169 4624 618
Size: 325 217 627 1382 1455 1842
Fragments arranged by size:
1842 1455 1382 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2037 2236 2331 2578 3167 1391
Size: 646 199 95 247 589 4072
Fragments arranged by size:
4072 646 589 247 199 95
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4877
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4039 4073 1903
Size: 34 2102 34 3678
Fragments arranged by size:
3678 2102 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3740 4950 3740
Size: 1210 4638
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5848
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4459 136
Size: 107 122 71 631 605 2787 1525
Fragments arranged by size:
2787 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5843 5843
Size: 5848
BlpI GC'TnA_GC
Cuts at: 3265 3265
Size: 5848
Bme1580I G_kGCm'C
Cuts at: 1790 2037 2410 2578 2712 3909 5065 1790
Size: 247 373 168 134 1197 1156 2573
Fragments arranged by size:
2573 1197 1156 373 247 168 134
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5848
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2098 2933 3579
Size: 416 53 83 399 24 681 835 646
Cuts at: 3579 4537 442
Size: 958 1753
Fragments arranged by size:
1753 958 835 681 646 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5848
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2872 2872
Size: 5848
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2033 2603 2766 2903 3092 4508 1239
Size: 794 570 163 137 189 1416 2579
Fragments arranged by size:
2579 1416 794 570 189 163 137
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3973 4838 5136 3973
Size: 865 298 4685
Fragments arranged by size:
4685 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2729 2899 4511 882
Size: 617 1230 170 1612 2219
Fragments arranged by size:
2219 1612 1230 617 170
BsaAI yAC'GTr
Cuts at: 493 1204 3039 3639 493
Size: 711 1835 600 2702
Fragments arranged by size:
2702 1835 711 600
BsaBI GATnn'nnATC
Cuts at: 5842 5842
Size: 5848
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3655 4037 4672 275
Size: 53 83 186 936 2122 382 635 1451
Fragments arranged by size:
2122 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4281 4679 4953 5100 5511 4281
Size: 398 274 147 411 4618
Fragments arranged by size:
4618 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5623
Fragments arranged by size:
5623 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2244 2546 2882 2887 3125 1186
Size: 1058 302 336 5 238 3909
Fragments arranged by size:
3909 1058 336 302 238 5
BseYI C'CCAG_C
Cuts at: 5051 5547 5051
Size: 496 5352
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2191 2237 2346 2351 1163
Size: 573 455 46 109 5 4660
Fragments arranged by size:
4660 573 455 109 46 5
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2984 3298 4059 5087 1540
Size: 275 186 983 314 761 1028 2301
Fragments arranged by size:
2301 1028 983 761 314 275 186
BsiHKAI G_wGCw'C
Cuts at: 2499 3909 3994 5065 2499
Size: 1410 85 1071 3282
Fragments arranged by size:
3282 1410 1071 85
BsmI GAATG_Cn'
Cuts at: 3359 3452 3359
Size: 93 5755
BsmBI CGTCTCn'nnnn_
Cuts at: 2237 2237
Size: 5848
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2236 2331 2410 2499 2578 2712
Size: 399 247 199 95 79 89 79 134
Cuts at: 2712 3167 3909 3994 5065 1391
Size: 455 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 455 399 247 199 134
95 89 85 79 79
BspEI T'CCGG_A
Cuts at: 5511 5511
Size: 5848
BspHI T'CATG_A
Cuts at: 3737 5467 3737
Size: 1730 4118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2342 2664 2697 3021 3203 844
Size: 1498 322 33 324 182 3489
Fragments arranged by size:
3489 1498 324 322 182 33
BsrBI CCG'CTC
Cuts at: 1394 1823 3735 1394
Size: 429 1912 3507
Fragments arranged by size:
3507 1912 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4343 4517 65
Size: 1183 3095 174 1396
Fragments arranged by size:
3095 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2979 4492 4679 1700
Size: 110 1169 1513 187 2869
Fragments arranged by size:
2869 1513 1169 187 110
BsrGI T'GTAC_A
Cuts at: 96 2649 96
Size: 2553 3295
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5848
BssSI C'ACGA_G
Cuts at: 3908 4920 3908
Size: 1012 4836
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2782 794
Size: 1988 3860
BstBI TT'CG_AA
Cuts at: 4652 4652
Size: 5848
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5848
BstYI r'GATC_y
Cuts at: 1972 3178 3545 3930 3947 5399 5485 5497 5843
Size: 1206 367 385 17 1452 86 12 346
Cuts at: 5843 1972
Size: 1977
Fragments arranged by size:
1977 1452 1206 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2022 2022
Size: 5848
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4357
Fragments arranged by size:
4357 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2356 487
Size: 683 56 1130 3979
Fragments arranged by size:
3979 1130 683 56
BtsI GCAGTG_nn'
Cuts at: 2147 2363 2451 3364 4158 4178 2147
Size: 216 88 913 794 20 3817
Fragments arranged by size:
3817 913 794 216 88 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5848
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5813
DraI TTT'AAA
Cuts at: 3152 3152
Size: 5848
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4855 809
Size: 507 3539 1802
Fragments arranged by size:
3539 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2977 2981 3295
Size: 54 1425 50 275 18 1147 4 314
Cuts at: 3295 4184 5813 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1147 889 314 275 54 50
43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2981 3295 1537
Size: 275 1169 314 4090
Fragments arranged by size:
4090 1169 314 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2123 3778 1353
Size: 770 1655 3423
Fragments arranged by size:
3423 1655 770
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2450 4435 4809 4955 387
Size: 1064 138 861 1985 374 146 1280
Fragments arranged by size:
1985 1280 1064 861 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2033 2603 2766 2903 3092 3911 4508
Size: 393 401 570 163 137 189 819 597
Cuts at: 4508 5295 5563 1239
Size: 787 268 1524
Fragments arranged by size:
1524 819 787 597 570 401 393 268
189 163 137
EcoO109I rG'GnC_Cy
Cuts at: 2033 2574 2033
Size: 541 5307
EcoRI G'AATT_C
Cuts at: 3157 3535 3157
Size: 378 5470
FseI GG_CCGG'CC
Cuts at: 2983 2983
Size: 5848
FspI TGC'GCA
Cuts at: 4354 4354
Size: 5848
HaeII r_GCGC'y
Cuts at: 1678 2252 4995 1678
Size: 574 2743 2531
Fragments arranged by size:
2743 2531 574
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2553 2585 2858 2890 4489
Size: 32 135 32 981 32 273 32 1599
Cuts at: 4489 4521 4563 4595 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1599 981 273 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3192 3438 669
Size: 988 281 1254 246 3079
Fragments arranged by size:
3079 1254 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2108 748
Size: 1360 4488
HpaI GTT'AAC
Cuts at: 3438 3438
Size: 5848
KpnI G_GTAC'C
Cuts at: 3173 3173
Size: 5848
MfeI C'AATT_G
Cuts at: 3447 3447
Size: 5848
MluI A'CGCG_T
Cuts at: 5724 5724
Size: 5848
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4962 5146 1305
Size: 3657 184 2007
Fragments arranged by size:
3657 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4026
Fragments arranged by size:
4026 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2765 3806 4165 4324 518
Size: 648 1599 1041 359 159 2042
Fragments arranged by size:
2042 1599 1041 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3264 3941 5089 5334 1606
Size: 1658 677 1148 245 2120
Fragments arranged by size:
2120 1658 1148 677 245
NaeI GCC'GGC
Cuts at: 2981 2981
Size: 5848
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4357
Fragments arranged by size:
4357 939 552
NdeI CA'TA_TG
Cuts at: 387 2115 387
Size: 1728 4120
NgoMIV G'CCGG_C
Cuts at: 2979 2979
Size: 5848
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5848
NotI GC'GGCC_GC
Cuts at: 3295 3295
Size: 5848
NspI r_CATG'y
Cuts at: 1132 1229 2340 3206 4751 1132
Size: 97 1111 866 1545 2229
Fragments arranged by size:
2229 1545 1111 866 97
PasI CC'CwG_GG
Cuts at: 1348 2236 2587 1348
Size: 888 351 4609
Fragments arranged by size:
4609 888 351
PciI A'CATG_T
Cuts at: 2336 4747 2336
Size: 2411 3437
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2101 2926 1108
Size: 993 825 4030
Fragments arranged by size:
4030 993 825
PfoI T'CCnGG_A
Cuts at: 2880 2880
Size: 5848
PmeI GTTT'AAAC
Cuts at: 3152 3152
Size: 5848
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3917 3949 5430 5462 3917
Size: 32 1481 32 4303
Fragments arranged by size:
4303 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5848
PsiI TTA'TAA
Cuts at: 3418 3418
Size: 5848
PspOMI G'GGCC_C
Cuts at: 2033 2574 2033
Size: 541 5307
PstI C_TGCA'G
Cuts at: 830 2124 2694 3200 830
Size: 1294 570 506 3478
Fragments arranged by size:
3478 1294 570 506
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5848
SalI G'TCGA_C
Cuts at: 1936 3190 1936
Size: 1254 4594
SbfI CC_TGCA'GG
Cuts at: 3200 3200
Size: 5848
ScaI AGT'ACT
Cuts at: 4096 5808 4096
Size: 1712 4136
SexAI A'CCwGG_T
Cuts at: 2397 2397
Size: 5848
SfcI C'TryA_G
Cuts at: 826 1045 2120 2690 3122 3196 4331 5012 5203
Size: 219 1075 570 432 74 1135 681 191
Cuts at: 5203 826
Size: 1471
Fragments arranged by size:
1471 1135 1075 681 570 432 219 191
74
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5848
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5848
SmaI CCC'GGG
Cuts at: 1872 2038 3175 1872
Size: 166 1137 4545
Fragments arranged by size:
4545 1137 166
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3059 3090 3952 4853 5115 820
Size: 197 926 1116 31 862 901 262 1553
Fragments arranged by size:
1553 1116 926 901 862 262 197 31
SnaBI TAC'GTA
Cuts at: 493 3639 493
Size: 3146 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5848
SphI G_CATG'C
Cuts at: 1132 3206 1132
Size: 2074 3774
SspI AAT'ATT
Cuts at: 5 52 3772 5
Size: 47 3720 2081
Fragments arranged by size:
3720 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5848
StyI C'CwwG_G
Cuts at: 513 1065 2004 2163 2289 3287 513
Size: 552 939 159 126 998 3074
Fragments arranged by size:
3074 998 939 552 159 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3875 3892 4045 4230 4675 325
Size: 1255 2295 17 153 185 445 1498
Fragments arranged by size:
2295 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2649 4094 5806
Size: 274 80 33 51 1443 672 1445 1712
Cuts at: 5806 96
Size: 138
Fragments arranged by size:
1712 1445 1443 672 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2489 2521 5808 5840 2489
Size: 32 3287 32 2497
Fragments arranged by size:
3287 2497 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5848
XbaI T'CTAG_A
Cuts at: 2137 3184 2137
Size: 1047 4801
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2810 2810
Size: 5848
XhoI C'TCGA_G
Cuts at: 1943 3090 1943
Size: 1147 4701
XmaI C'CCGG_G
Cuts at: 1870 2036 3173 1870
Size: 166 1137 4545
Fragments arranged by size:
4545 1137 166
XmnI GAAnn'nnTTC
Cuts at: 3977 3977
Size: 5848
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3656 276
Size: 53 83 186 936 2122 2468
Fragments arranged by size:
2468 2122 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV
FalI FspAI KasI NarI NruI NsiI PacI PmlI
PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI