Restriction map for
pFN21AE5542
(Circular) (Six-base) MAPSORT of: pFN21AE5542.seq Check: 5813 from: 1 to: 5800
pFN21AE5542 5800 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3059 3059
Size: 5800
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3610 278
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3143 1937
Size: 1206 4594
Acc65I G'GTAC_C
Cuts at: 3033 3121 3033
Size: 88 5712
AclI AA'CG_TT
Cuts at: 1563 3927 4300 1563
Size: 2364 373 3063
Fragments arranged by size:
3063 2364 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2477 2651 3863 5247 5515 1632
Size: 845 174 1212 1384 268 1917
Fragments arranged by size:
1917 1384 1212 845 268 174
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5603
AflIII A'CryG_T
Cuts at: 1203 3027 4699 5676 1203
Size: 1824 1672 977 1327
Fragments arranged by size:
1824 1672 1327 977
AgeI A'CCGG_T
Cuts at: 4631 4631
Size: 5800
AhdI GACnn_n'nnGTC
Cuts at: 4529 4529
Size: 5800
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5800
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5800
AlwNI CAG_nnn'CTG
Cuts at: 3543 5115 3543
Size: 1572 4228
ApaLI G'TGCA_C
Cuts at: 3857 5013 3857
Size: 1156 4644
ApoI r'AATT_y
Cuts at: 1493 2095 2473 2907 3109 3335 3487 5596 1493
Size: 602 378 434 202 226 152 2109 1697
Fragments arranged by size:
2109 1697 602 434 378 226 202 152
AseI AT'TA_AT
Cuts at: 160 1032 4354 160
Size: 872 3322 1606
Fragments arranged by size:
3322 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
AvaI C'yCGr_G
Cuts at: 1870 1943 3063 3125 1870
Size: 73 1120 62 4545
Fragments arranged by size:
4545 1120 73 62
BamHI G'GATC_C
Cuts at: 3130 3497 3130
Size: 367 5433
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3033 3121 4576 618
Size: 325 217 627 1246 88 1455 1842
Fragments arranged by size:
1842 1455 1246 627 325 217 88
BanII G_rGCy'C
Cuts at: 1391 3119 1391
Size: 1728 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4829
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2421 2455 3991 4025 1903
Size: 34 484 34 1536 34 3678
Fragments arranged by size:
3678 1536 484 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3692 4902 3692
Size: 1210 4590
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5800
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2742 4411 136
Size: 107 122 71 631 605 1070 1669 1525
Fragments arranged by size:
1669 1525 1070 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5795 5795
Size: 5800
BlpI GC'TnA_GC
Cuts at: 3217 3217
Size: 5800
Bme1580I G_kGCm'C
Cuts at: 1790 3861 5017 1790
Size: 2071 1156 2573
Fragments arranged by size:
2573 2071 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5800
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3531 4489 442
Size: 416 53 83 399 24 2114 958 1753
Fragments arranged by size:
2114 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2357 2615 4460 1239
Size: 1118 258 1845 2579
Fragments arranged by size:
2579 1845 1118 258
Bpu10I CC'TnA_GC
Cuts at: 2481 2481
Size: 5800
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3925 4790 5088 3925
Size: 865 298 4637
Fragments arranged by size:
4637 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2822 4463 882
Size: 617 1323 1641 2219
Fragments arranged by size:
2219 1641 1323 617
BsaAI yAC'GTr
Cuts at: 493 1204 3591 493
Size: 711 2387 2702
Fragments arranged by size:
2702 2387 711
BsaBI GATnn'nnATC
Cuts at: 5794 5794
Size: 5800
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3607 3989 4624 275
Size: 53 83 186 936 2074 382 635 1451
Fragments arranged by size:
2074 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2131 4233 4631 4905 5052 5463 2131
Size: 2102 398 274 147 411 2468
Fragments arranged by size:
2468 2102 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2336 2366 2588 2618 2957
Size: 30 165 30 933 30 222 30 339
Cuts at: 2957 2987 2996 3026 1178
Size: 30 9 30 3952
Fragments arranged by size:
3952 933 339 222 165 30 30 30
30 30 30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5800
BseYI C'CCAG_C
Cuts at: 2355 5003 5499 2355
Size: 2648 496 2656
Fragments arranged by size:
2656 2648 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2689 1163
Size: 573 953 4274
Fragments arranged by size:
4274 953 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2746 3250 4011 5039 1540
Size: 275 186 745 504 761 1028 2301
Fragments arranged by size:
2301 1028 761 745 504 275 186
BsiHKAI G_wGCw'C
Cuts at: 3861 3946 5017 3861
Size: 85 1071 4644
Fragments arranged by size:
4644 1071 85
BsmI GAATG_Cn'
Cuts at: 3311 3404 3311
Size: 93 5707
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3119 3861 3946 5017 1391
Size: 399 1329 742 85 1071 2174
Fragments arranged by size:
2174 1329 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5463 5463
Size: 5800
BspHI T'CATG_A
Cuts at: 3689 5419 3689
Size: 1730 4070
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2934 3059 3155 844
Size: 2090 125 96 3489
Fragments arranged by size:
3489 2090 125 96
BsrBI CCG'CTC
Cuts at: 1394 1823 3687 1394
Size: 429 1864 3507
Fragments arranged by size:
3507 1864 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2984 4295 4469 65
Size: 1183 1736 1311 174 1396
Fragments arranged by size:
1736 1396 1311 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4444 4631 1700
Size: 110 2634 187 2869
Fragments arranged by size:
2869 2634 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5800
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5800
BssSI C'ACGA_G
Cuts at: 3860 4872 3860
Size: 1012 4788
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2857 2874 794
Size: 2063 17 3720
Fragments arranged by size:
3720 2063 17
BstBI TT'CG_AA
Cuts at: 4604 4604
Size: 5800
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5800
BstXI CCAn_nnnn'nTGG
Cuts at: 2528 3022 2528
Size: 494 5306
BstYI r'GATC_y
Cuts at: 1972 2822 3130 3497 3882 3899 5351 5437 5449
Size: 850 308 367 385 17 1452 86 12
Cuts at: 5449 5795 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 850 385 367 346 308 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5248
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5061
Fragments arranged by size:
5061 683 56
BtsI GCAGTG_nn'
Cuts at: 2122 3316 4110 4130 2122
Size: 1194 794 20 3792
Fragments arranged by size:
3792 1194 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5800
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5765
DraI TTT'AAA
Cuts at: 3104 3104
Size: 5800
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4807 809
Size: 507 3491 1802
Fragments arranged by size:
3491 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2743 3247 4136
Size: 54 1425 50 275 18 913 504 889
Cuts at: 4136 5765 8
Size: 1629 43
Fragments arranged by size:
1629 1425 913 889 504 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2743 3247 1537
Size: 275 931 504 4090
Fragments arranged by size:
4090 931 504 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2634 3730 1353
Size: 1281 1096 3423
Fragments arranged by size:
3423 1281 1096
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2026 4387 4761 4907 387
Size: 1064 138 437 2361 374 146 1280
Fragments arranged by size:
2361 1280 1064 437 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2357 2477 2615 2651 3863 4460 5247
Size: 393 725 120 138 36 1212 597 787
Cuts at: 5247 5515 1239
Size: 268 1524
Fragments arranged by size:
1524 1212 787 725 597 393 268 138
120 36
EcoNI CCTnn'n_nnAGG
Cuts at: 3066 3066
Size: 5800
EcoRI G'AATT_C
Cuts at: 2095 3109 3487 2095
Size: 1014 378 4408
Fragments arranged by size:
4408 1014 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2830 2830
Size: 5800
FspI TGC'GCA
Cuts at: 4306 4306
Size: 5800
HaeII r_GCGC'y
Cuts at: 1678 4947 1678
Size: 3269 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2474 2506 2560 2592 2996
Size: 32 135 32 902 32 54 32 404
Cuts at: 2996 3028 4441 4473 4515 4547 1373
Size: 32 1413 32 42 32 2626
Fragments arranged by size:
2626 1413 902 404 135 54 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2773 3144 3390 669
Size: 988 281 835 371 246 3079
Fragments arranged by size:
3079 988 835 371 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5800
HpaI GTT'AAC
Cuts at: 3390 3390
Size: 5800
KpnI G_GTAC'C
Cuts at: 3037 3125 3037
Size: 88 5712
MfeI C'AATT_G
Cuts at: 2110 3399 2110
Size: 1289 4511
MluI A'CGCG_T
Cuts at: 5676 5676
Size: 5800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4914 5098 1305
Size: 3609 184 2007
Fragments arranged by size:
3609 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3978
Fragments arranged by size:
3978 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3020 3758 4117 4276 518
Size: 648 1854 738 359 159 2042
Fragments arranged by size:
2042 1854 738 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2524 3216 3893 5041 5286 1606
Size: 918 692 677 1148 245 2120
Fragments arranged by size:
2120 1148 918 692 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5248
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5800
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5800
NotI GC'GGCC_GC
Cuts at: 3247 3247
Size: 5800
NspI r_CATG'y
Cuts at: 1132 1229 2166 3031 3158 4703 1132
Size: 97 937 865 127 1545 2229
Fragments arranged by size:
2229 1545 937 865 127 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5800
PciI A'CATG_T
Cuts at: 3027 4699 3027
Size: 1672 4128
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5800
PfoI T'CCnGG_A
Cuts at: 2709 2709
Size: 5800
PmeI GTTT'AAAC
Cuts at: 3104 3104
Size: 5800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3869 3901 5382 5414 3869
Size: 32 1481 32 4255
Fragments arranged by size:
4255 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5800
PsiI TTA'TAA
Cuts at: 2217 3370 2217
Size: 1153 4647
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2327 2359 2327
Size: 32 5768
PstI C_TGCA'G
Cuts at: 830 2862 2931 3152 830
Size: 2032 69 221 3478
Fragments arranged by size:
3478 2032 221 69
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5800
PvuII CAG'CTG
Cuts at: 2524 2524
Size: 5800
SalI G'TCGA_C
Cuts at: 1936 3142 1936
Size: 1206 4594
SapI GCTCTTCn'nnn_
Cuts at: 2634 2634
Size: 5800
SbfI CC_TGCA'GG
Cuts at: 3152 3152
Size: 5800
ScaI AGT'ACT
Cuts at: 2882 4048 5760 2882
Size: 1166 1712 2922
Fragments arranged by size:
2922 1712 1166
SfcI C'TryA_G
Cuts at: 826 1045 2858 2927 3148 4283 4964 5155 826
Size: 219 1813 69 221 1135 681 191 1471
Fragments arranged by size:
1813 1471 1135 681 221 219 191 69
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5800
SmaI CCC'GGG
Cuts at: 1872 3127 1872
Size: 1255 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3904 4805 5067 820
Size: 197 926 1961 901 262 1553
Fragments arranged by size:
1961 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3591 493
Size: 3098 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5800
SphI G_CATG'C
Cuts at: 1132 2166 3158 1132
Size: 1034 992 3774
Fragments arranged by size:
3774 1034 992
SspI AAT'ATT
Cuts at: 5 52 3724 5
Size: 47 3672 2081
Fragments arranged by size:
3672 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5800
StyI C'CwwG_G
Cuts at: 513 1065 3239 513
Size: 552 2174 3074
Fragments arranged by size:
3074 2174 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3827 3844 3997 4182 4627 325
Size: 1255 2247 17 153 185 445 1498
Fragments arranged by size:
2247 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2880 4046 5758
Size: 274 80 33 51 1443 903 1166 1712
Cuts at: 5758 96
Size: 138
Fragments arranged by size:
1712 1443 1166 903 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2588 2620 2941 2973 5760 5792 2588
Size: 32 321 32 2787 32 2596
Fragments arranged by size:
2787 2596 321 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5800
XbaI T'CTAG_A
Cuts at: 3136 3136
Size: 5800
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2590 2590
Size: 5800
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5800
XmaI C'CCGG_G
Cuts at: 1870 3125 1870
Size: 1255 4545
XmnI GAAnn'nnTTC
Cuts at: 2042 3929 2042
Size: 1887 3913
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3608 276
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI