Restriction map for
pFN21AE5538
(Circular) (Six-base) MAPSORT of: pFN21AE5538.seq Check: 8532 from: 1 to: 5800
pFN21AE5538 5800 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3610 278
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3143 1937
Size: 1206 4594
Acc65I G'GTAC_C
Cuts at: 3121 3121
Size: 5800
AclI AA'CG_TT
Cuts at: 1563 3927 4300 1563
Size: 2364 373 3063
Fragments arranged by size:
3063 2364 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3863 5247 5515 1632
Size: 2231 1384 268 1917
Fragments arranged by size:
2231 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5603
AflIII A'CryG_T
Cuts at: 1203 4699 5676 1203
Size: 3496 977 1327
Fragments arranged by size:
3496 1327 977
AgeI A'CCGG_T
Cuts at: 4631 4631
Size: 5800
AhdI GACnn_n'nnGTC
Cuts at: 4529 4529
Size: 5800
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5800
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5800
AlwNI CAG_nnn'CTG
Cuts at: 2137 2918 3543 5115 2137
Size: 781 625 1572 2822
Fragments arranged by size:
2822 1572 781 625
ApaLI G'TGCA_C
Cuts at: 3857 5013 3857
Size: 1156 4644
ApoI r'AATT_y
Cuts at: 1493 2479 2689 2992 3109 3335 3487 5596 1493
Size: 986 210 303 117 226 152 2109 1697
Fragments arranged by size:
2109 1697 986 303 226 210 152 117
AseI AT'TA_AT
Cuts at: 160 1032 4354 160
Size: 872 3322 1606
Fragments arranged by size:
3322 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
AvaI C'yCGr_G
Cuts at: 1870 1943 3125 1870
Size: 73 1182 4545
Fragments arranged by size:
4545 1182 73
AvrII C'CTAG_G
Cuts at: 2032 2032
Size: 5800
BamHI G'GATC_C
Cuts at: 2457 3130 3497 2457
Size: 673 367 4760
Fragments arranged by size:
4760 673 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2975 3121 4576 618
Size: 325 217 627 1188 146 1455 1842
Fragments arranged by size:
1842 1455 1188 627 325 217 146
BanII G_rGCy'C
Cuts at: 1391 3119 1391
Size: 1728 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2020 3043 928
Size: 971 121 1023 3685
Fragments arranged by size:
3685 1023 971 121
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3991 4025 1903
Size: 34 2054 34 3678
Fragments arranged by size:
3678 2054 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3692 4902 3692
Size: 1210 4590
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5800
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4411 136
Size: 107 122 71 631 605 2739 1525
Fragments arranged by size:
2739 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5795 5795
Size: 5800
BlpI GC'TnA_GC
Cuts at: 3217 3217
Size: 5800
Bme1580I G_kGCm'C
Cuts at: 1790 2978 3861 5017 1790
Size: 1188 883 1156 2573
Fragments arranged by size:
2573 1188 1156 883
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5800
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2196 2777 3531
Size: 416 53 83 399 24 779 581 754
Cuts at: 3531 4489 442
Size: 958 1753
Fragments arranged by size:
1753 958 779 754 581 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5800
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2734 2734
Size: 5800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2036 2907 4460 1239
Size: 797 871 1553 2579
Fragments arranged by size:
2579 1553 871 797
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3925 4790 5088 3925
Size: 865 298 4637
Fragments arranged by size:
4637 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4463 882
Size: 617 2964 2219
Fragments arranged by size:
2964 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3591 493
Size: 711 2387 2702
Fragments arranged by size:
2702 2387 711
BsaBI GATnn'nnATC
Cuts at: 5794 5794
Size: 5800
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3607 3989 4624 275
Size: 53 83 186 936 2074 382 635 1451
Fragments arranged by size:
2074 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4233 4631 4905 5052 5463 4233
Size: 398 274 147 411 4570
Fragments arranged by size:
4570 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5575
Fragments arranged by size:
5575 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2141 1186
Size: 955 4845
BseYI C'CCAG_C
Cuts at: 2124 2130 5003 5499 2124
Size: 6 2873 496 2425
Fragments arranged by size:
2873 2425 496 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5227
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3250 4011 5039 1540
Size: 275 186 1249 761 1028 2301
Fragments arranged by size:
2301 1249 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3087 3861 3946 5017 3087
Size: 774 85 1071 3870
Fragments arranged by size:
3870 1071 774 85
BsmI GAATG_Cn'
Cuts at: 3311 3404 3311
Size: 93 5707
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2978 3087 3119 3861 3946 5017 1391
Size: 399 1188 109 32 742 85 1071 2174
Fragments arranged by size:
2174 1188 1071 742 399 109 85 32
BspEI T'CCGG_A
Cuts at: 5463 5463
Size: 5800
BspHI T'CATG_A
Cuts at: 2802 3689 5419 2802
Size: 887 1730 3183
Fragments arranged by size:
3183 1730 887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3155 844
Size: 2311 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3687 1394
Size: 429 1864 3507
Fragments arranged by size:
3507 1864 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2443 4295 4469 65
Size: 1183 1195 1852 174 1396
Fragments arranged by size:
1852 1396 1195 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4444 4631 1700
Size: 110 2634 187 2869
Fragments arranged by size:
2869 2634 187 110
BsrGI T'GTAC_A
Cuts at: 96 2556 96
Size: 2460 3340
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5800
BssSI C'ACGA_G
Cuts at: 2495 3860 4872 2495
Size: 1365 1012 3423
Fragments arranged by size:
3423 1365 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5800
BstBI TT'CG_AA
Cuts at: 4604 4604
Size: 5800
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5800
BstXI CCAn_nnnn'nTGG
Cuts at: 2524 2524
Size: 5800
BstYI r'GATC_y
Cuts at: 1972 2457 3130 3497 3882 3899 5351 5437 5449
Size: 485 673 367 385 17 1452 86 12
Cuts at: 5449 5795 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 673 485 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5248
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5061
Fragments arranged by size:
5061 683 56
BtsI GCAGTG_nn'
Cuts at: 3316 4110 4130 3316
Size: 794 20 4986
Fragments arranged by size:
4986 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5800
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5765
DraI TTT'AAA
Cuts at: 3104 3104
Size: 5800
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2798 4807 809
Size: 507 1482 2009 1802
Fragments arranged by size:
2009 1802 1482 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3247 4136 5765
Size: 54 1425 50 275 18 1417 889 1629
Cuts at: 5765 8
Size: 43
Fragments arranged by size:
1629 1425 1417 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3247 1537
Size: 275 1435 4090
Fragments arranged by size:
4090 1435 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2104 2735 3730 1353
Size: 751 631 995 3423
Fragments arranged by size:
3423 995 751 631
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4387 4761 4907 387
Size: 1064 138 2798 374 146 1280
Fragments arranged by size:
2798 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2907 3863 4460 5247 5515 1239
Size: 393 404 871 956 597 787 268 1524
Fragments arranged by size:
1524 956 871 787 597 404 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2156 2473 2156
Size: 317 5483
EcoO109I rG'GnC_Cy
Cuts at: 2069 2490 2069
Size: 421 5379
EcoRI G'AATT_C
Cuts at: 2479 3109 3487 2479
Size: 630 378 4792
Fragments arranged by size:
4792 630 378
FspI TGC'GCA
Cuts at: 4306 4306
Size: 5800
HaeII r_GCGC'y
Cuts at: 1678 4947 1678
Size: 3269 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2577 2609 2641 4441 4473
Size: 32 135 32 1005 32 32 1800 32
Cuts at: 4473 4515 4547 1373
Size: 42 32 2626
Fragments arranged by size:
2626 1800 1005 135 42 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2172 2314 3144 3390 669
Size: 988 281 234 142 830 246 3079
Fragments arranged by size:
3079 988 830 281 246 234 142
HindIII A'AGCT_T
Cuts at: 748 2696 748
Size: 1948 3852
HpaI GTT'AAC
Cuts at: 3390 3390
Size: 5800
KpnI G_GTAC'C
Cuts at: 3125 3125
Size: 5800
MfeI C'AATT_G
Cuts at: 3399 3399
Size: 5800
MluI A'CGCG_T
Cuts at: 5676 5676
Size: 5800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3051 4914 5098 1305
Size: 1746 1863 184 2007
Fragments arranged by size:
2007 1863 1746 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3978
Fragments arranged by size:
3978 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2499 2801 3758 4117 4276 518
Size: 648 1333 302 957 359 159 2042
Fragments arranged by size:
2042 1333 957 648 359 302 159
MspA1I CmG'CkG
Cuts at: 1606 3216 3893 5041 5286 1606
Size: 1610 677 1148 245 2120
Fragments arranged by size:
2120 1610 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5248
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5800
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5800
NotI GC'GGCC_GC
Cuts at: 3247 3247
Size: 5800
NspI r_CATG'y
Cuts at: 1132 1229 2602 3158 4703 1132
Size: 97 1373 556 1545 2229
Fragments arranged by size:
2229 1545 1373 556 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5800
PciI A'CATG_T
Cuts at: 4699 4699
Size: 5800
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2137 1108
Size: 1029 4771
PfoI T'CCnGG_A
Cuts at: 2460 2720 2460
Size: 260 5540
PmeI GTTT'AAAC
Cuts at: 3104 3104
Size: 5800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3869 3901 5382 5414 3869
Size: 32 1481 32 4255
Fragments arranged by size:
4255 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2069 2490 2069
Size: 421 5379
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5800
PsiI TTA'TAA
Cuts at: 3370 3370
Size: 5800
PstI C_TGCA'G
Cuts at: 830 3152 830
Size: 2322 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5800
SalI G'TCGA_C
Cuts at: 1936 3142 1936
Size: 1206 4594
SapI GCTCTTCn'nnn_
Cuts at: 2735 2735
Size: 5800
SbfI CC_TGCA'GG
Cuts at: 3152 3152
Size: 5800
ScaI AGT'ACT
Cuts at: 4048 5760 4048
Size: 1712 4088
SexAI A'CCwGG_T
Cuts at: 2378 2378
Size: 5800
SfcI C'TryA_G
Cuts at: 826 1045 3148 4283 4964 5155 826
Size: 219 2103 1135 681 191 1471
Fragments arranged by size:
2103 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5800
SmaI CCC'GGG
Cuts at: 1872 3127 1872
Size: 1255 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3904 4805 5067 820
Size: 197 926 1961 901 262 1553
Fragments arranged by size:
1961 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3591 493
Size: 3098 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5800
SphI G_CATG'C
Cuts at: 1132 3158 1132
Size: 2026 3774
SspI AAT'ATT
Cuts at: 5 52 3724 5
Size: 47 3672 2081
Fragments arranged by size:
3672 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5800
StyI C'CwwG_G
Cuts at: 513 1065 2032 2320 3021 3239 513
Size: 552 967 288 701 218 3074
Fragments arranged by size:
3074 967 701 552 288 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3827 3844 3997 4182 4627 325
Size: 1255 2247 17 153 185 445 1498
Fragments arranged by size:
2247 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2556 4046 5758
Size: 274 80 33 51 1443 579 1490 1712
Cuts at: 5758 96
Size: 138
Fragments arranged by size:
1712 1490 1443 579 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5760 5792 5760
Size: 32 5768
Tth111I GACn'n_nGTC
Cuts at: 1316 2914 1316
Size: 1598 4202
XbaI T'CTAG_A
Cuts at: 3136 3136
Size: 5800
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2968 2968
Size: 5800
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5800
XmaI C'CCGG_G
Cuts at: 1870 3125 1870
Size: 1255 4545
XmnI GAAnn'nnTTC
Cuts at: 2729 3929 2729
Size: 1200 4600
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3608 276
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI