Restriction map for
pFN21AE5537
(Circular) (Six-base) MAPSORT of: pFN21AE5537.seq Check: 1574 from: 1 to: 6013
pFN21AE5537 6013 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3823 278
Size: 53 83 186 936 2287 2468
Fragments arranged by size:
2468 2287 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3150 3356 1937
Size: 1213 206 4594
Fragments arranged by size:
4594 1213 206
Acc65I G'GTAC_C
Cuts at: 3334 3334
Size: 6013
AclI AA'CG_TT
Cuts at: 1563 4140 4513 1563
Size: 2577 373 3063
Fragments arranged by size:
3063 2577 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2059 2389 2555 3169 4076 5460 5728 1632
Size: 427 330 166 614 907 1384 268 1917
Fragments arranged by size:
1917 1384 907 614 427 330 268 166
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5816
AflIII A'CryG_T
Cuts at: 1203 4912 5889 1203
Size: 3709 977 1327
Fragments arranged by size:
3709 1327 977
AgeI A'CCGG_T
Cuts at: 4844 4844
Size: 6013
AhdI GACnn_n'nnGTC
Cuts at: 4742 4742
Size: 6013
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6013
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6013
AlwNI CAG_nnn'CTG
Cuts at: 2341 2823 3756 5328 2341
Size: 482 933 1572 3026
Fragments arranged by size:
3026 1572 933 482
ApaI G_GGCC'C
Cuts at: 2889 2889
Size: 6013
ApaLI G'TGCA_C
Cuts at: 4070 5226 4070
Size: 1156 4857
ApoI r'AATT_y
Cuts at: 1493 2110 2490 2586 3322 3548 3700 5809 1493
Size: 617 380 96 736 226 152 2109 1697
Fragments arranged by size:
2109 1697 736 617 380 226 152 96
AseI AT'TA_AT
Cuts at: 160 1032 4567 160
Size: 872 3535 1606
Fragments arranged by size:
3535 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6013
AvaI C'yCGr_G
Cuts at: 1870 1943 2171 2595 3338 1870
Size: 73 228 424 743 4545
Fragments arranged by size:
4545 743 424 228 73
BamHI G'GATC_C
Cuts at: 3343 3710 3343
Size: 367 5646
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2559 3334 4789 618
Size: 325 217 627 772 775 1455 1842
Fragments arranged by size:
1842 1455 775 772 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2712 2889 3021 3198 3332 1391
Size: 1321 177 132 177 134 4072
Fragments arranged by size:
4072 1321 177 177 134 132
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2065 2362 928
Size: 971 166 297 4579
Fragments arranged by size:
4579 971 297 166
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4204 4238 1903
Size: 34 50 34 2183 34 3678
Fragments arranged by size:
3678 2183 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3905 5115 3905
Size: 1210 4803
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6013
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4624 136
Size: 107 122 71 631 605 2952 1525
Fragments arranged by size:
2952 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6008 6008
Size: 6013
BlpI GC'TnA_GC
Cuts at: 3430 3430
Size: 6013
Bme1580I G_kGCm'C
Cuts at: 1790 2015 2889 4074 5230 1790
Size: 225 874 1185 1156 2573
Fragments arranged by size:
2573 1185 1156 874 225
BmgBI CAC'GTC
Cuts at: 1318 3264 1318
Size: 1946 4067
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3163 3216 3744
Size: 416 53 83 399 24 1746 53 528
Cuts at: 3744 4702 442
Size: 958 1753
Fragments arranged by size:
1753 1746 958 528 416 399 83 53
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6013
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2040 2201 2312 2405 2450 2568 3035 4673
Size: 801 161 111 93 45 118 467 1638
Cuts at: 4673 1239
Size: 2579
Fragments arranged by size:
2579 1638 801 467 161 118 111 93
45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4138 5003 5301 4138
Size: 865 298 4850
Fragments arranged by size:
4850 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4676 882
Size: 617 3177 2219
Fragments arranged by size:
3177 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3804 493
Size: 711 2600 2702
Fragments arranged by size:
2702 2600 711
BsaBI GATnn'nnATC
Cuts at: 2966 6007 2966
Size: 3041 2972
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3820 4202 4837 275
Size: 53 83 186 936 2287 382 635 1451
Fragments arranged by size:
2287 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4446 4844 5118 5265 5676 4446
Size: 398 274 147 411 4783
Fragments arranged by size:
4783 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2873 2903 3059 3089 1178
Size: 30 165 30 1470 30 156 30 4102
Fragments arranged by size:
4102 1470 165 156 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2447 1186
Size: 1261 4752
BseYI C'CCAG_C
Cuts at: 2221 2231 2563 5216 5712 2221
Size: 10 332 2653 496 2522
Fragments arranged by size:
2653 2522 496 332 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5440
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3007 3463 4224 5252 1540
Size: 275 186 1006 456 761 1028 2301
Fragments arranged by size:
2301 1028 1006 761 456 275 186
BsiHKAI G_wGCw'C
Cuts at: 2712 3198 3294 4074 4159 5230 2712
Size: 486 96 780 85 1071 3495
Fragments arranged by size:
3495 1071 780 486 96 85
BsmI GAATG_Cn'
Cuts at: 2470 2692 3524 3617 2470
Size: 222 832 93 4866
Fragments arranged by size:
4866 832 222 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3254 3254
Size: 6013
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2712 2889 3021 3198 3294 3332
Size: 399 225 697 177 132 177 96 38
Cuts at: 3332 4074 4159 5230 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 697 399 225 177 177
132 96 85 38
BspEI T'CCGG_A
Cuts at: 5676 5676
Size: 6013
BspHI T'CATG_A
Cuts at: 2319 3902 5632 2319
Size: 1583 1730 2700
Fragments arranged by size:
2700 1730 1583
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3368 844
Size: 2524 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3900 1394
Size: 429 2077 3507
Fragments arranged by size:
3507 2077 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4508 4682 65
Size: 1183 3260 174 1396
Fragments arranged by size:
3260 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2379 2618 3229 4657 4844 1700
Size: 110 569 239 611 1428 187 2869
Fragments arranged by size:
2869 1428 611 569 239 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6013
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6013
BssSI C'ACGA_G
Cuts at: 4073 5085 4073
Size: 1012 5001
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2865 794
Size: 2071 3942
BstBI TT'CG_AA
Cuts at: 4817 4817
Size: 6013
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6013
BstXI CCAn_nnnn'nTGG
Cuts at: 2571 2571
Size: 6013
BstYI r'GATC_y
Cuts at: 1972 2176 2809 3343 3710 4095 4112 5564 5650
Size: 204 633 534 367 385 17 1452 86
Cuts at: 5650 5662 6008 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 633 534 385 367 346 204
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2049 2364 2691 2941 3085 3249 513
Size: 552 984 315 327 250 144 164 3277
Fragments arranged by size:
3277 984 552 327 315 250 164 144
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5274
Fragments arranged by size:
5274 683 56
BtsI GCAGTG_nn'
Cuts at: 3529 4323 4343 3529
Size: 794 20 5199
Fragments arranged by size:
5199 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6013
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5978
DraI TTT'AAA
Cuts at: 3317 3317
Size: 6013
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5020 809
Size: 507 3704 1802
Fragments arranged by size:
3704 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2142 3004 3132
Size: 54 1425 50 275 18 312 862 128
Cuts at: 3132 3231 3460 4349 5978 8
Size: 99 229 889 1629 43
Fragments arranged by size:
1629 1425 889 862 312 275 229 128
99 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3004 3460 1537
Size: 275 1192 456 4090
Fragments arranged by size:
4090 1192 456 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3943 1353
Size: 2590 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2074 2779 4600 4974 5120 387
Size: 1064 138 485 705 1821 374 146 1280
Fragments arranged by size:
1821 1280 1064 705 485 374 146 138
EcoICRI GAG'CTC
Cuts at: 2710 3196 2710
Size: 486 5527
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2040 2059 2201 2312 2389 2405 2450
Size: 393 408 19 142 111 77 16 45
Cuts at: 2450 2555 2568 3035 3169 4076 4673 5460 5728
Size: 105 13 467 134 907 597 787 268
Cuts at: 5728 1239
Size: 1524
Fragments arranged by size:
1524 907 787 597 467 408 393 268
142 134 111 105 77 45 19 16
13
EcoO109I rG'GnC_Cy
Cuts at: 2185 2886 2185
Size: 701 5312
EcoRI G'AATT_C
Cuts at: 2586 3322 3700 2586
Size: 736 378 4899
Fragments arranged by size:
4899 736 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2224 3199 2224
Size: 975 5038
FspI TGC'GCA
Cuts at: 4519 4519
Size: 6013
HaeII r_GCGC'y
Cuts at: 1678 3275 5160 1678
Size: 1597 1885 2531
Fragments arranged by size:
2531 1885 1597
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2468 2500 2775 2807 4654
Size: 32 135 32 896 32 275 32 1847
Cuts at: 4654 4686 4728 4760 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1847 896 275 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3151 3357 3603 669
Size: 988 281 1213 206 246 3079
Fragments arranged by size:
3079 1213 988 281 246 206
HindIII A'AGCT_T
Cuts at: 748 2283 748
Size: 1535 4478
HpaI GTT'AAC
Cuts at: 3603 3603
Size: 6013
KpnI G_GTAC'C
Cuts at: 3338 3338
Size: 6013
MfeI C'AATT_G
Cuts at: 3612 3612
Size: 6013
MluI A'CGCG_T
Cuts at: 5889 5889
Size: 6013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2880 5127 5311 1305
Size: 1575 2247 184 2007
Fragments arranged by size:
2247 2007 1575 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2144 3134 10
Size: 54 1425 343 312 990 2889
Fragments arranged by size:
2889 1425 990 343 312 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2336 2804 3971 4330 4489 518
Size: 648 1170 468 1167 359 159 2042
Fragments arranged by size:
2042 1170 1167 648 468 359 159
MspA1I CmG'CkG
Cuts at: 1606 3429 4106 5254 5499 1606
Size: 1823 677 1148 245 2120
Fragments arranged by size:
2120 1823 1148 677 245
NaeI GCC'GGC
Cuts at: 2381 3231 2381
Size: 850 5163
NcoI C'CATG_G
Cuts at: 513 1065 2364 2691 2941 3085 513
Size: 552 1299 327 250 144 3441
Fragments arranged by size:
3441 1299 552 327 250 144
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6013
NgoMIV G'CCGG_C
Cuts at: 2379 3229 2379
Size: 850 5163
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6013
NotI GC'GGCC_GC
Cuts at: 3460 3460
Size: 6013
NspI r_CATG'y
Cuts at: 1132 1229 3371 4916 1132
Size: 97 2142 1545 2229
Fragments arranged by size:
2229 2142 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2036 1348
Size: 688 5325
PciI A'CATG_T
Cuts at: 4912 4912
Size: 6013
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2203 2428 1108
Size: 1095 225 4693
Fragments arranged by size:
4693 1095 225
PfoI T'CCnGG_A
Cuts at: 2017 3198 2017
Size: 1181 4832
PmeI GTTT'AAAC
Cuts at: 3317 3317
Size: 6013
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4082 4114 5595 5627 4082
Size: 32 1481 32 4468
Fragments arranged by size:
4468 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6013
PsiI TTA'TAA
Cuts at: 3583 3583
Size: 6013
PspOMI G'GGCC_C
Cuts at: 2885 2885
Size: 6013
PstI C_TGCA'G
Cuts at: 830 3365 830
Size: 2535 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6013
SacI G_AGCT'C
Cuts at: 2712 3198 2712
Size: 486 5527
SalI G'TCGA_C
Cuts at: 1936 3149 3355 1936
Size: 1213 206 4594
Fragments arranged by size:
4594 1213 206
SbfI CC_TGCA'GG
Cuts at: 3365 3365
Size: 6013
ScaI AGT'ACT
Cuts at: 4261 5973 4261
Size: 1712 4301
SexAI A'CCwGG_T
Cuts at: 2124 2124
Size: 6013
SfcI C'TryA_G
Cuts at: 826 1045 3361 4496 5177 5368 826
Size: 219 2316 1135 681 191 1471
Fragments arranged by size:
2316 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6013
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6013
SmaI CCC'GGG
Cuts at: 1872 2597 3340 1872
Size: 725 743 4545
Fragments arranged by size:
4545 743 725
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4117 5018 5280 820
Size: 197 926 2174 901 262 1553
Fragments arranged by size:
2174 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3804 493
Size: 3311 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6013
SphI G_CATG'C
Cuts at: 1132 3371 1132
Size: 2239 3774
SspI AAT'ATT
Cuts at: 5 52 3937 5
Size: 47 3885 2081
Fragments arranged by size:
3885 2081 47
StuI AGG'CCT
Cuts at: 1517 3071 1517
Size: 1554 4459
StyI C'CwwG_G
Cuts at: 513 1065 2364 2691 2941 3054 3085 3452 513
Size: 552 1299 327 250 113 31 367 3074
Fragments arranged by size:
3074 1299 552 367 327 250 113 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2577 2624 4040 4057 4210 4395 4840
Size: 1255 997 47 1416 17 153 185 445
Cuts at: 4840 325
Size: 1498
Fragments arranged by size:
1498 1416 1255 997 445 185 153 47
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3096 4259 5971
Size: 274 80 33 51 1443 1119 1163 1712
Cuts at: 5971 96
Size: 138
Fragments arranged by size:
1712 1443 1163 1119 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2873 2905 3228 3260 5973 6005 2873
Size: 32 323 32 2713 32 2881
Fragments arranged by size:
2881 2713 323 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2666 1316
Size: 1350 4663
XbaI T'CTAG_A
Cuts at: 3349 3349
Size: 6013
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2239 3061 3127 2239
Size: 822 66 5125
Fragments arranged by size:
5125 822 66
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6013
XmaI C'CCGG_G
Cuts at: 1870 2595 3338 1870
Size: 725 743 4545
Fragments arranged by size:
4545 743 725
XmnI GAAnn'nnTTC
Cuts at: 2848 4142 2848
Size: 1294 4719
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3821 276
Size: 53 83 186 936 2287 2468
Fragments arranged by size:
2468 2287 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI
SapI SfiI SfoI SrfI SwaI