Restriction map for
pFN21AE5535
(Circular) (Six-base) MAPSORT of: pFN21AE5535.seq Check: 8303 from: 1 to: 6070
pFN21AE5535 6070 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3880 278
Size: 53 83 186 936 2344 2468
Fragments arranged by size:
2468 2344 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3413 1937
Size: 1476 4594
Acc65I G'GTAC_C
Cuts at: 2195 3391 2195
Size: 1196 4874
AclI AA'CG_TT
Cuts at: 1563 4197 4570 1563
Size: 2634 373 3063
Fragments arranged by size:
3063 2634 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2035 2732 3245 4133 5517 5785 1632
Size: 403 697 513 888 1384 268 1917
Fragments arranged by size:
1917 1384 888 697 513 403 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5873
AflIII A'CryG_T
Cuts at: 1203 2182 4969 5946 1203
Size: 979 2787 977 1327
Fragments arranged by size:
2787 1327 979 977
AgeI A'CCGG_T
Cuts at: 3311 4901 3311
Size: 1590 4480
AhdI GACnn_n'nnGTC
Cuts at: 4799 4799
Size: 6070
AleI CACnn'nnGTG
Cuts at: 1166 2834 2918 1166
Size: 1668 84 4318
Fragments arranged by size:
4318 1668 84
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6070
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2592 2624 3212 3244 2592
Size: 32 588 32 5418
Fragments arranged by size:
5418 588 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2092 2435 3813 5385 2092
Size: 343 1378 1572 2777
Fragments arranged by size:
2777 1572 1378 343
ApaI G_GGCC'C
Cuts at: 2986 2986
Size: 6070
ApaLI G'TGCA_C
Cuts at: 4127 5283 4127
Size: 1156 4914
ApoI r'AATT_y
Cuts at: 1493 3379 3605 3757 5866 1493
Size: 1886 226 152 2109 1697
Fragments arranged by size:
2109 1886 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4624 160
Size: 872 3592 1606
Fragments arranged by size:
3592 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6070
AvaI C'yCGr_G
Cuts at: 1870 1943 2322 2395 2496 2766 2978 3212 3395
Size: 73 379 73 101 270 212 234 183
Cuts at: 3395 1870
Size: 4545
Fragments arranged by size:
4545 379 270 234 212 183 101 73
73
AvrII C'CTAG_G
Cuts at: 2890 2890
Size: 6070
BamHI G'GATC_C
Cuts at: 3400 3767 3400
Size: 367 5703
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2195 2828 3308 3391 4846
Size: 325 217 627 408 633 480 83 1455
Cuts at: 4846 618
Size: 1842
Fragments arranged by size:
1842 1455 633 627 480 408 325 217
83
BanII G_rGCy'C
Cuts at: 1391 2323 2889 2986 3389 1391
Size: 932 566 97 403 4072
Fragments arranged by size:
4072 932 566 403 97
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2165 2228 928
Size: 971 266 63 4770
Fragments arranged by size:
4770 971 266 63
BbvCI CC'TCA_GC
Cuts at: 2259 3089 2259
Size: 830 5240
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4261 4295 1903
Size: 34 2324 34 3678
Fragments arranged by size:
3678 2324 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3962 5172 3962
Size: 1210 4860
BclI T'GATC_A
Cuts at: 1548 2424 1548
Size: 876 5194
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2150 4681 136
Size: 107 122 71 631 605 478 2531 1525
Fragments arranged by size:
2531 1525 631 605 478 122 107 71
BglII A'GATC_T
Cuts at: 6065 6065
Size: 6070
BlpI GC'TnA_GC
Cuts at: 3487 3487
Size: 6070
Bme1580I G_kGCm'C
Cuts at: 1790 2683 2763 2986 4131 5287 1790
Size: 893 80 223 1145 1156 2573
Fragments arranged by size:
2573 1156 1145 893 223 80
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6070
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2648 2750 3801
Size: 416 53 83 399 24 1231 102 1051
Cuts at: 3801 4759 442
Size: 958 1753
Fragments arranged by size:
1753 1231 1051 958 416 399 102 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6070
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2993 2993
Size: 6070
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2624 2795 2972 4730 1239
Size: 1385 171 177 1758 2579
Fragments arranged by size:
2579 1758 1385 177 171
Bpu10I CC'TnA_GC
Cuts at: 2259 3089 2259
Size: 830 5240
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2705 4195 5060 5358 2705
Size: 1490 865 298 3417
Fragments arranged by size:
3417 1490 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2063 2642 4733 882
Size: 617 564 579 2091 2219
Fragments arranged by size:
2219 2091 617 579 564
BsaAI yAC'GTr
Cuts at: 493 1204 2167 3861 493
Size: 711 963 1694 2702
Fragments arranged by size:
2702 1694 963 711
BsaBI GATnn'nnATC
Cuts at: 6064 6064
Size: 6070
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2042 3877 4259 4894
Size: 53 83 186 936 509 1835 382 635
Cuts at: 4894 275
Size: 1451
Fragments arranged by size:
1835 1451 936 635 509 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3297 3311 4503 4901 5175 5322 5733 3297
Size: 14 1192 398 274 147 411 3634
Fragments arranged by size:
3634 1192 411 398 274 147 14
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2792 2822 1178
Size: 30 165 30 1389 30 4426
Fragments arranged by size:
4426 1389 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2306 2327 2660 3359 3362 1186
Size: 1120 21 333 699 3 3894
Fragments arranged by size:
3894 1120 699 333 21 3
BseYI C'CCAG_C
Cuts at: 5273 5769 5273
Size: 496 5574
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2421 2741 1163
Size: 573 685 320 4492
Fragments arranged by size:
4492 685 573 320
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2022 2071 3520 4281 5309 1540
Size: 275 186 21 49 1449 761 1028 2301
Fragments arranged by size:
2301 1449 1028 761 275 186 49 21
BsiHKAI G_wGCw'C
Cuts at: 2323 2505 2889 4131 4216 5287 2323
Size: 182 384 1242 85 1071 3106
Fragments arranged by size:
3106 1242 1071 384 182 85
BsmI GAATG_Cn'
Cuts at: 3581 3674 3581
Size: 93 5977
BsmBI CGTCTCn'nnnn_
Cuts at: 2868 2868
Size: 6070
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2323 2505 2683 2763 2889 2986 3389
Size: 399 533 182 178 80 126 97 403
Cuts at: 3389 4131 4216 5287 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 533 403 399 182 178
126 97 85 80
BspEI T'CCGG_A
Cuts at: 5733 5733
Size: 6070
BspHI T'CATG_A
Cuts at: 3959 5689 3959
Size: 1730 4340
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3425 844
Size: 2581 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3957 1394
Size: 429 2134 3507
Fragments arranged by size:
3507 2134 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2117 4565 4739 65
Size: 1183 869 2448 174 1396
Fragments arranged by size:
2448 1396 1183 869 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2066 3311 4714 4901 1700
Size: 110 256 1245 1403 187 2869
Fragments arranged by size:
2869 1403 1245 256 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6070
BssHII G'CGCG_C
Cuts at: 1925 2035 1925
Size: 110 5960
BssSI C'ACGA_G
Cuts at: 4130 5142 4130
Size: 1012 5058
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6070
BstBI TT'CG_AA
Cuts at: 4874 4874
Size: 6070
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6070
BstXI CCAn_nnnn'nTGG
Cuts at: 3331 3331
Size: 6070
BstYI r'GATC_y
Cuts at: 1972 2607 3400 3767 4152 4169 5621 5707 5719
Size: 635 793 367 385 17 1452 86 12
Cuts at: 5719 6065 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 793 635 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2134 2145 2215 2824 2832 513
Size: 552 1069 11 70 609 8 3751
Fragments arranged by size:
3751 1069 609 552 70 11 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5331
Fragments arranged by size:
5331 683 56
BtsI GCAGTG_nn'
Cuts at: 2361 2433 3586 4380 4400 2361
Size: 72 1153 794 20 4031
Fragments arranged by size:
4031 1153 794 72 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6070
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6035
DraI TTT'AAA
Cuts at: 2865 3374 2865
Size: 509 5561
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5077 809
Size: 507 3761 1802
Fragments arranged by size:
3761 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2019 2068 2142
Size: 54 1425 50 275 18 189 49 74
Cuts at: 2142 2203 2514 2821 3106 3333 3517 4406 6035
Size: 61 311 307 285 227 184 889 1629
Cuts at: 6035 8
Size: 43
Fragments arranged by size:
1629 1425 889 311 307 285 275 227
189 184 74 61 54 50 49 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2019 2068 3517 1537
Size: 275 207 49 1449 4090
Fragments arranged by size:
4090 1449 275 207 49
EarI CTCTTCn'nnn_
Cuts at: 1353 4000 1353
Size: 2647 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4657 5031 5177 387
Size: 1064 138 3068 374 146 1280
Fragments arranged by size:
3068 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2321 2887 2321
Size: 566 5504
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2035 2624 2732 2795 2972 3245 4133
Size: 393 403 589 108 63 177 273 888
Cuts at: 4133 4730 5517 5785 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 888 787 597 589 403 393 273
268 177 108 63
EcoNI CCTnn'n_nnAGG
Cuts at: 2084 2703 3092 2084
Size: 619 389 5062
Fragments arranged by size:
5062 619 389
EcoO109I rG'GnC_Cy
Cuts at: 2695 2770 2982 2983 3022 3220 2695
Size: 75 212 1 39 198 5545
Fragments arranged by size:
5545 212 198 75 39 1
EcoRI G'AATT_C
Cuts at: 3379 3757 3379
Size: 378 5692
FspI TGC'GCA
Cuts at: 4576 4576
Size: 6070
HaeII r_GCGC'y
Cuts at: 1678 5217 1678
Size: 3539 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4711 4743 4785 4817 1373
Size: 32 135 32 3139 32 42 32 2626
Fragments arranged by size:
3139 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3414 3660 669
Size: 988 281 1476 246 3079
Fragments arranged by size:
3079 1476 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6070
HpaI GTT'AAC
Cuts at: 3660 3660
Size: 6070
KpnI G_GTAC'C
Cuts at: 2199 3395 2199
Size: 1196 4874
MfeI C'AATT_G
Cuts at: 3669 3669
Size: 6070
MluI A'CGCG_T
Cuts at: 5946 5946
Size: 6070
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3155 5184 5368 1305
Size: 1850 2029 184 2007
Fragments arranged by size:
2029 2007 1850 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2516 3335 10
Size: 54 1425 343 684 819 2745
Fragments arranged by size:
2745 1425 819 684 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2187 2459 2756 2834 2918 2933 4028
Size: 648 1021 272 297 78 84 15 1095
Cuts at: 4028 4387 4546 518
Size: 359 159 2042
Fragments arranged by size:
2042 1095 1021 648 359 297 272 159
84 78 15
MspA1I CmG'CkG
Cuts at: 1606 2055 2136 3486 4163 5311 5556 1606
Size: 449 81 1350 677 1148 245 2120
Fragments arranged by size:
2120 1350 1148 677 449 245 81
NaeI GCC'GGC
Cuts at: 2068 2068
Size: 6070
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5518
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6070
NgoMIV G'CCGG_C
Cuts at: 2066 2066
Size: 6070
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6070
NotI GC'GGCC_GC
Cuts at: 2019 3517 2019
Size: 1498 4572
NspI r_CATG'y
Cuts at: 1132 1229 2186 2540 3428 4973 1132
Size: 97 957 354 888 1545 2229
Fragments arranged by size:
2229 1545 957 888 354 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6070
PciI A'CATG_T
Cuts at: 2182 4969 2182
Size: 2787 3283
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3067 1108
Size: 1959 4111
PfoI T'CCnGG_A
Cuts at: 2571 2610 2571
Size: 39 6031
PmeI GTTT'AAAC
Cuts at: 3374 3374
Size: 6070
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4139 4171 5652 5684 4139
Size: 32 1481 32 4525
Fragments arranged by size:
4525 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2695 2770 3220 2695
Size: 75 450 5545
Fragments arranged by size:
5545 450 75
PshAI GACnn'nnGTC
Cuts at: 1613 2654 1613
Size: 1041 5029
PsiI TTA'TAA
Cuts at: 3640 3640
Size: 6070
PspOMI G'GGCC_C
Cuts at: 2982 2982
Size: 6070
PstI C_TGCA'G
Cuts at: 830 2088 2239 3422 830
Size: 1258 151 1183 3478
Fragments arranged by size:
3478 1258 1183 151
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6070
SacI G_AGCT'C
Cuts at: 2323 2889 2323
Size: 566 5504
SacII CC_GC'GG
Cuts at: 2137 2137
Size: 6070
SalI G'TCGA_C
Cuts at: 1936 3412 1936
Size: 1476 4594
SanDI GG'GwC_CC
Cuts at: 2695 2695
Size: 6070
SbfI CC_TGCA'GG
Cuts at: 3422 3422
Size: 6070
ScaI AGT'ACT
Cuts at: 4318 6030 4318
Size: 1712 4358
SfcI C'TryA_G
Cuts at: 826 1045 2084 2235 3418 4553 5234 5425 826
Size: 219 1039 151 1183 1135 681 191 1471
Fragments arranged by size:
1471 1183 1135 1039 681 219 191 151
SfiI GGCCn_nnn'nGGCC
Cuts at: 2150 2150
Size: 6070
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6070
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6070
SmaI CCC'GGG
Cuts at: 1872 2397 2768 3214 3397 1872
Size: 525 371 446 183 4545
Fragments arranged by size:
4545 525 446 371 183
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2684 4174 5075 5337 820
Size: 197 926 741 1490 901 262 1553
Fragments arranged by size:
1553 1490 926 901 741 262 197
SnaBI TAC'GTA
Cuts at: 493 3861 493
Size: 3368 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6070
SphI G_CATG'C
Cuts at: 1132 3428 1132
Size: 2296 3774
SspI AAT'ATT
Cuts at: 5 52 3994 5
Size: 47 3942 2081
Fragments arranged by size:
3942 2081 47
StuI AGG'CCT
Cuts at: 1517 2709 1517
Size: 1192 4878
StyI C'CwwG_G
Cuts at: 513 1065 2890 3509 513
Size: 552 1825 619 3074
Fragments arranged by size:
3074 1825 619 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4097 4114 4267 4452 4897 325
Size: 1255 2517 17 153 185 445 1498
Fragments arranged by size:
2517 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2808 4316 6028
Size: 274 80 33 51 1443 831 1508 1712
Cuts at: 6028 96
Size: 138
Fragments arranged by size:
1712 1508 1443 831 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6030 6062 6030
Size: 32 6038
Tth111I GACn'n_nGTC
Cuts at: 1316 2567 1316
Size: 1251 4819
XbaI T'CTAG_A
Cuts at: 3406 3406
Size: 6070
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2206 2011
Size: 195 5875
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6070
XmaI C'CCGG_G
Cuts at: 1870 2395 2766 3212 3395 1870
Size: 525 371 446 183 4545
Fragments arranged by size:
4545 525 446 371 183
XmnI GAAnn'nnTTC
Cuts at: 2783 4199 2783
Size: 1416 4654
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3878 276
Size: 53 83 186 936 2344 2468
Fragments arranged by size:
2468 2344 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SacI SacII SalI SanDI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BaeI BbeI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII
SapI SexAI SfoI SrfI SwaI