Restriction map for
pFN21AE5522
(Circular) (Six-base) MAPSORT of: pFN21AE5522.seq Check: 6439 from: 1 to: 5776
pFN21AE5522 5776 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3586 278
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2649 3119 1937
Size: 712 470 4594
Fragments arranged by size:
4594 712 470
Acc65I G'GTAC_C
Cuts at: 3097 3097
Size: 5776
AclI AA'CG_TT
Cuts at: 1563 3903 4276 1563
Size: 2340 373 3063
Fragments arranged by size:
3063 2340 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3839 5223 5491 1632
Size: 2207 1384 268 1917
Fragments arranged by size:
2207 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5579
AflIII A'CryG_T
Cuts at: 1203 2058 4675 5652 1203
Size: 855 2617 977 1327
Fragments arranged by size:
2617 1327 977 855
AgeI A'CCGG_T
Cuts at: 2505 4607 2505
Size: 2102 3674
AhdI GACnn_n'nnGTC
Cuts at: 4505 4505
Size: 5776
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5776
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5776
AlwNI CAG_nnn'CTG
Cuts at: 2584 2984 3519 5091 2584
Size: 400 535 1572 3269
Fragments arranged by size:
3269 1572 535 400
ApaLI G'TGCA_C
Cuts at: 2767 3833 4989 2767
Size: 1066 1156 3554
Fragments arranged by size:
3554 1156 1066
ApoI r'AATT_y
Cuts at: 1493 2690 3085 3311 3463 5572 1493
Size: 1197 395 226 152 2109 1697
Fragments arranged by size:
2109 1697 1197 395 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4330 160
Size: 872 3298 1606
Fragments arranged by size:
3298 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
AvaI C'yCGr_G
Cuts at: 1870 1943 2119 2201 2832 3101 1870
Size: 73 176 82 631 269 4545
Fragments arranged by size:
4545 631 269 176 82 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2564 2597 2564
Size: 33 5743
BamHI G'GATC_C
Cuts at: 3106 3473 3106
Size: 367 5409
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2141 2348 3038 3097 4552
Size: 325 217 627 354 207 690 59 1455
Cuts at: 4552 618
Size: 1842
Fragments arranged by size:
1842 1455 690 627 354 325 217 207
59
BanII G_rGCy'C
Cuts at: 1391 2465 2619 2715 3095 1391
Size: 1074 154 96 380 4072
Fragments arranged by size:
4072 1074 380 154 96
BbeI G_GCGC'C
Cuts at: 2145 2145
Size: 5776
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2428 2527 2788 928
Size: 971 529 99 261 3916
Fragments arranged by size:
3916 971 529 261 99
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3967 4001 1903
Size: 34 2030 34 3678
Fragments arranged by size:
3678 2030 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3668 4878 3668
Size: 1210 4566
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5776
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2266 4387 136
Size: 107 122 71 631 605 594 2121 1525
Fragments arranged by size:
2121 1525 631 605 594 122 107 71
BglII A'GATC_T
Cuts at: 5771 5771
Size: 5776
BlpI GC'TnA_GC
Cuts at: 2613 3193 2613
Size: 580 5196
Bme1580I G_kGCm'C
Cuts at: 1790 2771 3041 3837 4993 1790
Size: 981 270 796 1156 2573
Fragments arranged by size:
2573 1156 981 796 270
BmgBI CAC'GTC
Cuts at: 1318 2059 2953 1318
Size: 741 894 4141
Fragments arranged by size:
4141 894 741
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3507 4465 442
Size: 416 53 83 399 24 2090 958 1753
Fragments arranged by size:
2090 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2150 2681 4436 1239
Size: 911 531 1755 2579
Fragments arranged by size:
2579 1755 911 531
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2381 2699 3901 4766 5064 2381
Size: 318 1202 865 298 3093
Fragments arranged by size:
3093 1202 865 318 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2877 4439 882
Size: 617 1378 1562 2219
Fragments arranged by size:
2219 1562 1378 617
BsaAI yAC'GTr
Cuts at: 493 1204 3567 493
Size: 711 2363 2702
Fragments arranged by size:
2702 2363 711
BsaBI GATnn'nnATC
Cuts at: 5770 5770
Size: 5776
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2142 2793 3583 3965
Size: 53 83 186 936 609 651 790 382
Cuts at: 3965 4600 275
Size: 635 1451
Fragments arranged by size:
1451 936 790 651 635 609 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2505 4209 4607 4881 5028 5439 2505
Size: 1704 398 274 147 411 2842
Fragments arranged by size:
2842 1704 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2478 2508 1178
Size: 30 165 30 1075 30 4446
Fragments arranged by size:
4446 1075 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2486 1186
Size: 1300 4476
BseYI C'CCAG_C
Cuts at: 2326 2788 4979 5475 2326
Size: 462 2191 496 2627
Fragments arranged by size:
2627 2191 496 462
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2219 2735 1163
Size: 573 483 516 4204
Fragments arranged by size:
4204 573 516 483
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3226 3987 5015 1540
Size: 275 186 1225 761 1028 2301
Fragments arranged by size:
2301 1225 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2619 2715 2771 3837 3922 4993 2619
Size: 96 56 1066 85 1071 3402
Fragments arranged by size:
3402 1071 1066 96 85 56
BsmI GAATG_Cn'
Cuts at: 3287 3380 3287
Size: 93 5683
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2465 2619 2715 2771 3041 3095 3837
Size: 399 675 154 96 56 270 54 742
Cuts at: 3837 3922 4993 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 675 399 270 154 96
85 56 54
BspEI T'CCGG_A
Cuts at: 5439 5439
Size: 5776
BspHI T'CATG_A
Cuts at: 2619 3070 3665 5395 2619
Size: 451 595 1730 3000
Fragments arranged by size:
3000 1730 595 451
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2757 3131 844
Size: 1913 374 3489
Fragments arranged by size:
3489 1913 374
BsrBI CCG'CTC
Cuts at: 1394 1823 2206 2928 3663 1394
Size: 429 383 722 735 3507
Fragments arranged by size:
3507 735 722 429 383
BsrDI GCAATG_nn'
Cuts at: 65 1248 4271 4445 65
Size: 1183 3023 174 1396
Fragments arranged by size:
3023 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2505 4420 4607 1700
Size: 110 695 1915 187 2869
Fragments arranged by size:
2869 1915 695 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5776
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5776
BssSI C'ACGA_G
Cuts at: 2900 3836 4848 2900
Size: 936 1012 3828
Fragments arranged by size:
3828 1012 936
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5776
BstBI TT'CG_AA
Cuts at: 4580 4580
Size: 5776
BstEII G'GTnAC_C
Cuts at: 1184 2089 1184
Size: 905 4871
BstXI CCAn_nnnn'nTGG
Cuts at: 2017 2017
Size: 5776
BstYI r'GATC_y
Cuts at: 1972 3106 3473 3858 3875 5327 5413 5425 5771
Size: 1134 367 385 17 1452 86 12 346
Cuts at: 5771 1972
Size: 1977
Fragments arranged by size:
1977 1452 1134 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2145 2172 3046 513
Size: 552 1080 27 874 3243
Fragments arranged by size:
3243 1080 874 552 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5037
Fragments arranged by size:
5037 683 56
BtsI GCAGTG_nn'
Cuts at: 2769 3292 4086 4106 2769
Size: 523 794 20 4439
Fragments arranged by size:
4439 794 523 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5776
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5741
DraI TTT'AAA
Cuts at: 2428 3080 2428
Size: 652 5124
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4783 809
Size: 507 3467 1802
Fragments arranged by size:
3467 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2511 2850 2939
Size: 54 1425 50 275 18 681 339 89
Cuts at: 2939 3223 4112 5741 8
Size: 284 889 1629 43
Fragments arranged by size:
1629 1425 889 681 339 284 275 89
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3223 1537
Size: 275 1411 4090
Fragments arranged by size:
4090 1411 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3706 1353
Size: 2353 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2247 4363 4737 4883 387
Size: 1064 138 658 2116 374 146 1280
Fragments arranged by size:
2116 1280 1064 658 374 146 138
EcoICRI GAG'CTC
Cuts at: 2617 2713 2617
Size: 96 5680
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2150 2681 3839 4436 5223 5491 1239
Size: 393 518 531 1158 597 787 268 1524
Fragments arranged by size:
1524 1158 787 597 531 518 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2737 3016 2737
Size: 279 5497
EcoRI G'AATT_C
Cuts at: 3085 3463 3085
Size: 378 5398
FspI TGC'GCA
Cuts at: 4282 4282
Size: 5776
HaeII r_GCGC'y
Cuts at: 1678 2145 4923 1678
Size: 467 2778 2531
Fragments arranged by size:
2778 2531 467
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2069 2101 4417 4449 4491
Size: 32 135 32 497 32 2316 32 42
Cuts at: 4491 4523 1373
Size: 32 2626
Fragments arranged by size:
2626 2316 497 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2650 3120 3366 669
Size: 988 281 712 470 246 3079
Fragments arranged by size:
3079 988 712 470 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5776
HpaI GTT'AAC
Cuts at: 3366 3366
Size: 5776
KasI G'GCGC_C
Cuts at: 2141 2141
Size: 5776
KpnI G_GTAC'C
Cuts at: 3101 3101
Size: 5776
MfeI C'AATT_G
Cuts at: 3375 3375
Size: 5776
MluI A'CGCG_T
Cuts at: 5652 5652
Size: 5776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2955 3031 4890 5074 1305
Size: 1650 76 1859 184 2007
Fragments arranged by size:
2007 1859 1650 184 76
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2513 2941 10
Size: 54 1425 343 681 428 2845
Fragments arranged by size:
2845 1425 681 428 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2672 3734 4093 4252 518
Size: 648 861 645 1062 359 159 2042
Fragments arranged by size:
2042 1062 861 648 645 359 159
MspA1I CmG'CkG
Cuts at: 1606 2153 3048 3192 3869 5017 5262 1606
Size: 547 895 144 677 1148 245 2120
Fragments arranged by size:
2120 1148 895 677 547 245 144
NarI GG'CG_CC
Cuts at: 2142 2142
Size: 5776
NcoI C'CATG_G
Cuts at: 513 1065 2172 513
Size: 552 1107 4117
Fragments arranged by size:
4117 1107 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5776
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5776
NotI GC'GGCC_GC
Cuts at: 3223 3223
Size: 5776
NspI r_CATG'y
Cuts at: 1132 1229 2200 2219 3134 4679 1132
Size: 97 971 19 915 1545 2229
Fragments arranged by size:
2229 1545 971 915 97 19
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5776
PciI A'CATG_T
Cuts at: 4675 4675
Size: 5776
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5776
PfoI T'CCnGG_A
Cuts at: 2127 2697 2127
Size: 570 5206
PmeI GTTT'AAAC
Cuts at: 3080 3080
Size: 5776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3845 3877 5358 5390 3845
Size: 32 1481 32 4231
Fragments arranged by size:
4231 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2737 2737
Size: 5776
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5776
PsiI TTA'TAA
Cuts at: 3346 3346
Size: 5776
PspXI vC'TCGA_Gb
Cuts at: 2201 2201
Size: 5776
PstI C_TGCA'G
Cuts at: 830 2163 2580 3128 830
Size: 1333 417 548 3478
Fragments arranged by size:
3478 1333 548 417
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5776
SacI G_AGCT'C
Cuts at: 2619 2715 2619
Size: 96 5680
SacII CC_GC'GG
Cuts at: 3049 3049
Size: 5776
SalI G'TCGA_C
Cuts at: 1936 2648 3118 1936
Size: 712 470 4594
Fragments arranged by size:
4594 712 470
SbfI CC_TGCA'GG
Cuts at: 3128 3128
Size: 5776
ScaI AGT'ACT
Cuts at: 4024 5736 4024
Size: 1712 4064
SfcI C'TryA_G
Cuts at: 826 1045 2159 2576 3124 4259 4940 5131 826
Size: 219 1114 417 548 1135 681 191 1471
Fragments arranged by size:
1471 1135 1114 681 548 417 219 191
SfoI GGC'GCC
Cuts at: 2143 2143
Size: 5776
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5776
SmaI CCC'GGG
Cuts at: 1872 2834 3103 1872
Size: 962 269 4545
Fragments arranged by size:
4545 962 269
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2201 2396 2714 3880 4781 5043
Size: 197 926 258 195 318 1166 901 262
Cuts at: 5043 820
Size: 1553
Fragments arranged by size:
1553 1166 926 901 318 262 258 197
195
SnaBI TAC'GTA
Cuts at: 493 3567 493
Size: 3074 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5776
SphI G_CATG'C
Cuts at: 1132 2219 3134 1132
Size: 1087 915 3774
Fragments arranged by size:
3774 1087 915
SspI AAT'ATT
Cuts at: 5 52 2479 3700 5
Size: 47 2427 1221 2081
Fragments arranged by size:
2427 2081 1221 47
StuI AGG'CCT
Cuts at: 1517 2309 1517
Size: 792 4984
StyI C'CwwG_G
Cuts at: 513 1065 2172 2638 3215 513
Size: 552 1107 466 577 3074
Fragments arranged by size:
3074 1107 577 552 466
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2108 3803 3820 3973 4158 4603 325
Size: 1255 528 1695 17 153 185 445 1498
Fragments arranged by size:
1695 1498 1255 528 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4022 5734 96
Size: 274 80 33 51 1443 2045 1712 138
Fragments arranged by size:
2045 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5736 5768 5736
Size: 32 5744
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5776
XbaI T'CTAG_A
Cuts at: 3112 3112
Size: 5776
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2359 2996 2359
Size: 637 5139
XhoI C'TCGA_G
Cuts at: 1943 2201 1943
Size: 258 5518
XmaI C'CCGG_G
Cuts at: 1870 2832 3101 1870
Size: 962 269 4545
Fragments arranged by size:
4545 962 269
XmnI GAAnn'nnTTC
Cuts at: 3905 3905
Size: 5776
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3584 276
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspXI PstI PvuI SacI SacII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PmlI PspOMI PsrI PvuII RsrII SanDI SapI
SexAI SfiI SrfI SwaI