Restriction map for
pFN21AE5521
(Circular) (Six-base) MAPSORT of: pFN21AE5521.seq Check: 6092 from: 1 to: 6016
pFN21AE5521 6016 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3306 3306
Size: 6016
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3826 278
Size: 53 83 186 936 2290 2468
Fragments arranged by size:
2468 2290 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3087 3359 1937
Size: 1150 272 4594
Fragments arranged by size:
4594 1150 272
Acc65I G'GTAC_C
Cuts at: 3337 3337
Size: 6016
AclI AA'CG_TT
Cuts at: 1563 3125 4143 4516 1563
Size: 1562 1018 373 3063
Fragments arranged by size:
3063 1562 1018 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2192 2324 3062 4079 5463 5731 1632
Size: 560 132 738 1017 1384 268 1917
Fragments arranged by size:
1917 1384 1017 738 560 268 132
AflII C'TTAA_G
Cuts at: 820 1017 2192 820
Size: 197 1175 4644
Fragments arranged by size:
4644 1175 197
AflIII A'CryG_T
Cuts at: 1203 2487 4915 5892 1203
Size: 1284 2428 977 1327
Fragments arranged by size:
2428 1327 1284 977
AgeI A'CCGG_T
Cuts at: 4847 4847
Size: 6016
AhdI GACnn_n'nnGTC
Cuts at: 4745 4745
Size: 6016
AleI CACnn'nnGTG
Cuts at: 1166 2865 1166
Size: 1699 4317
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6016
AlwNI CAG_nnn'CTG
Cuts at: 2249 2900 3759 5331 2249
Size: 651 859 1572 2934
Fragments arranged by size:
2934 1572 859 651
ApaLI G'TGCA_C
Cuts at: 4073 5229 4073
Size: 1156 4860
ApoI r'AATT_y
Cuts at: 1493 2436 3325 3551 3703 5812 1493
Size: 943 889 226 152 2109 1697
Fragments arranged by size:
2109 1697 943 889 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4570 160
Size: 872 3538 1606
Fragments arranged by size:
3538 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6016
AvaI C'yCGr_G
Cuts at: 1870 1943 2015 2277 3341 1870
Size: 73 72 262 1064 4545
Fragments arranged by size:
4545 1064 262 73 72
BamHI G'GATC_C
Cuts at: 3346 3713 3346
Size: 367 5649
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3337 4792 618
Size: 325 217 627 1550 1455 1842
Fragments arranged by size:
1842 1550 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2284 3140 3335 1391
Size: 893 856 195 4072
Fragments arranged by size:
4072 893 856 195
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5045
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4207 4241 1903
Size: 34 2270 34 3678
Fragments arranged by size:
3678 2270 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2723 3009 3908 5118 2723
Size: 286 899 1210 3621
Fragments arranged by size:
3621 1210 899 286
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6016
BfrBI ATG'CAT
Cuts at: 2452 2978 3185 2452
Size: 526 207 5283
Fragments arranged by size:
5283 526 207
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4627 136
Size: 107 122 71 631 605 2955 1525
Fragments arranged by size:
2955 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2335 6011 2335
Size: 3676 2340
BlpI GC'TnA_GC
Cuts at: 3433 3433
Size: 6016
Bme1580I G_kGCm'C
Cuts at: 1790 2091 4077 5233 1790
Size: 301 1986 1156 2573
Fragments arranged by size:
2573 1986 1156 301
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6016
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3747 4705 442
Size: 416 53 83 399 24 2330 958 1753
Fragments arranged by size:
2330 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6016
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2372 4676 1239
Size: 1133 2304 2579
Fragments arranged by size:
2579 2304 1133
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2770 4141 5006 5304 2770
Size: 1371 865 298 3482
Fragments arranged by size:
3482 1371 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4679 882
Size: 617 3180 2219
Fragments arranged by size:
3180 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3203 3807 493
Size: 711 1999 604 2702
Fragments arranged by size:
2702 1999 711 604
BsaBI GATnn'nnATC
Cuts at: 3242 6010 3242
Size: 2768 3248
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3823 4205 4840 275
Size: 53 83 186 936 2290 382 635 1451
Fragments arranged by size:
2290 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4449 4847 5121 5268 5679 4449
Size: 398 274 147 411 4786
Fragments arranged by size:
4786 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5791
Fragments arranged by size:
5791 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6016
BseYI C'CCAG_C
Cuts at: 2213 2539 2665 5219 5715 2213
Size: 326 126 2554 496 2514
Fragments arranged by size:
2554 2514 496 326 126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5443
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3466 4227 5255 1540
Size: 275 186 1465 761 1028 2301
Fragments arranged by size:
2301 1465 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3140 4077 4162 5233 3140
Size: 937 85 1071 3923
Fragments arranged by size:
3923 1071 937 85
BsmI GAATG_Cn'
Cuts at: 3076 3527 3620 3076
Size: 451 93 5472
Fragments arranged by size:
5472 451 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2284 3140 3335 4077 4162 5233
Size: 399 301 193 856 195 742 85 1071
Cuts at: 5233 1391
Size: 2174
Fragments arranged by size:
2174 1071 856 742 399 301 195 193
85
BspEI T'CCGG_A
Cuts at: 5679 5679
Size: 6016
BspHI T'CATG_A
Cuts at: 3905 5635 3905
Size: 1730 4286
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3306 3371 844
Size: 2462 65 3489
Fragments arranged by size:
3489 2462 65
BsrBI CCG'CTC
Cuts at: 1394 1823 3903 1394
Size: 429 2080 3507
Fragments arranged by size:
3507 2080 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4511 4685 65
Size: 1183 3263 174 1396
Fragments arranged by size:
3263 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4660 4847 1700
Size: 110 2850 187 2869
Fragments arranged by size:
2869 2850 187 110
BsrGI T'GTAC_A
Cuts at: 96 3257 96
Size: 3161 2855
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6016
BssSI C'ACGA_G
Cuts at: 4076 5088 4076
Size: 1012 5004
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6016
BstBI TT'CG_AA
Cuts at: 2935 3195 4820 2935
Size: 260 1625 4131
Fragments arranged by size:
4131 1625 260
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6016
BstYI r'GATC_y
Cuts at: 1972 2335 2390 3346 3713 4098 4115 5567 5653
Size: 363 55 956 367 385 17 1452 86
Cuts at: 5653 5665 6011 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 956 385 367 363 346 86
55 17 12
BstZ17I GTA'TAC
Cuts at: 3088 3088
Size: 6016
BtgI C'CryG_G
Cuts at: 513 1065 2800 513
Size: 552 1735 3729
Fragments arranged by size:
3729 1735 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5277
Fragments arranged by size:
5277 683 56
BtsI GCAGTG_nn'
Cuts at: 3532 4326 4346 3532
Size: 794 20 5202
Fragments arranged by size:
5202 794 20
ClaI AT'CG_AT
Cuts at: 1554 2755 1554
Size: 1201 4815
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5981
DraI TTT'AAA
Cuts at: 2535 3320 2535
Size: 785 5231
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5023 809
Size: 507 3707 1802
Fragments arranged by size:
3707 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3463 4352 5981
Size: 54 1425 50 275 18 1633 889 1629
Cuts at: 5981 8
Size: 43
Fragments arranged by size:
1633 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3463 1537
Size: 275 1651 4090
Fragments arranged by size:
4090 1651 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2148 2649 3946 1353
Size: 795 501 1297 3423
Fragments arranged by size:
3423 1297 795 501
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4603 4977 5123 387
Size: 1064 138 3014 374 146 1280
Fragments arranged by size:
3014 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3138 3138
Size: 6016
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2192 2324 2372 3062 4079 4676 5463
Size: 393 560 132 48 690 1017 597 787
Cuts at: 5463 5731 1239
Size: 268 1524
Fragments arranged by size:
1524 1017 787 690 597 560 393 268
132 48
EcoO109I rG'GnC_Cy
Cuts at: 2323 2323
Size: 6016
EcoRI G'AATT_C
Cuts at: 3325 3703 3325
Size: 378 5638
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2660 2660
Size: 6016
FspI TGC'GCA
Cuts at: 4522 4522
Size: 6016
HaeII r_GCGC'y
Cuts at: 1678 5163 1678
Size: 3485 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2440 2472 2541 2573 2737
Size: 32 135 32 868 32 69 32 164
Cuts at: 2737 2769 3213 3245 4657 4689 4731 4763 1373
Size: 32 444 32 1412 32 42 32 2626
Fragments arranged by size:
2626 1412 868 444 164 135 69 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3360 3606 669
Size: 988 281 1422 246 3079
Fragments arranged by size:
3079 1422 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6016
HpaI GTT'AAC
Cuts at: 3606 3606
Size: 6016
KpnI G_GTAC'C
Cuts at: 3341 3341
Size: 6016
MfeI C'AATT_G
Cuts at: 3615 3615
Size: 6016
MluI A'CGCG_T
Cuts at: 5892 5892
Size: 6016
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2723 5130 5314 1305
Size: 1418 2407 184 2007
Fragments arranged by size:
2407 2007 1418 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4194
Fragments arranged by size:
4194 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2486 2711 2865 2979 3974 4333 4492
Size: 648 1320 225 154 114 995 359 159
Cuts at: 4492 518
Size: 2042
Fragments arranged by size:
2042 1320 995 648 359 225 159 154
114
MspA1I CmG'CkG
Cuts at: 1606 2039 3432 4109 5257 5502 1606
Size: 433 1393 677 1148 245 2120
Fragments arranged by size:
2120 1393 1148 677 433 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5464
NdeI CA'TA_TG
Cuts at: 387 2164 2509 2980 387
Size: 1777 345 471 3423
Fragments arranged by size:
3423 1777 471 345
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6016
NotI GC'GGCC_GC
Cuts at: 3463 3463
Size: 6016
NsiI A_TGCA'T
Cuts at: 2454 2980 3187 2454
Size: 526 207 5283
Fragments arranged by size:
5283 526 207
NspI r_CATG'y
Cuts at: 1132 1229 2491 3374 4919 1132
Size: 97 1262 883 1545 2229
Fragments arranged by size:
2229 1545 1262 883 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6016
PciI A'CATG_T
Cuts at: 2487 4915 2487
Size: 2428 3588
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6016
PmeI GTTT'AAAC
Cuts at: 3320 3320
Size: 6016
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4085 4117 5598 5630 4085
Size: 32 1481 32 4471
Fragments arranged by size:
4471 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2323 2323
Size: 6016
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6016
PsiI TTA'TAA
Cuts at: 2103 3060 3586 2103
Size: 957 526 4533
Fragments arranged by size:
4533 957 526
PstI C_TGCA'G
Cuts at: 830 3055 3368 830
Size: 2225 313 3478
Fragments arranged by size:
3478 2225 313
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6016
PvuII CAG'CTG
Cuts at: 2039 2039
Size: 6016
SacI G_AGCT'C
Cuts at: 3140 3140
Size: 6016
SalI G'TCGA_C
Cuts at: 1936 3358 1936
Size: 1422 4594
SapI GCTCTTCn'nnn_
Cuts at: 2649 2649
Size: 6016
SbfI CC_TGCA'GG
Cuts at: 3368 3368
Size: 6016
ScaI AGT'ACT
Cuts at: 4264 5976 4264
Size: 1712 4304
SfcI C'TryA_G
Cuts at: 826 1045 2919 3051 3364 4499 5180 5371 826
Size: 219 1874 132 313 1135 681 191 1471
Fragments arranged by size:
1874 1471 1135 681 313 219 191 132
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6016
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6016
SmaI CCC'GGG
Cuts at: 1872 3343 1872
Size: 1471 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2192 2785 4120 5021 5283 820
Size: 197 926 249 593 1335 901 262 1553
Fragments arranged by size:
1553 1335 926 901 593 262 249 197
SnaBI TAC'GTA
Cuts at: 493 3807 493
Size: 3314 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6016
SphI G_CATG'C
Cuts at: 1132 3374 1132
Size: 2242 3774
SspI AAT'ATT
Cuts at: 5 52 3940 5
Size: 47 3888 2081
Fragments arranged by size:
3888 2081 47
StuI AGG'CCT
Cuts at: 1517 2478 1517
Size: 961 5055
StyI C'CwwG_G
Cuts at: 513 1065 3455 513
Size: 552 2390 3074
Fragments arranged by size:
3074 2390 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2390 4043 4060 4213 4398 4843 325
Size: 1255 810 1653 17 153 185 445 1498
Fragments arranged by size:
1653 1498 1255 810 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2316 3257 4262
Size: 274 80 33 51 1443 339 941 1005
Cuts at: 4262 5974 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1005 941 339 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2566 2598 5976 6008 2566
Size: 32 3378 32 2574
Fragments arranged by size:
3378 2574 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6016
XbaI T'CTAG_A
Cuts at: 3352 3352
Size: 6016
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6016
XmaI C'CCGG_G
Cuts at: 1870 3341 1870
Size: 1471 4545
XmnI GAAnn'nnTTC
Cuts at: 2337 2436 2643 4145 2337
Size: 99 207 1502 4208
Fragments arranged by size:
4208 1502 207 99
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3824 276
Size: 53 83 186 936 2290 2468
Fragments arranged by size:
2468 2290 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI