Restriction map for
pFN21AE5520
(Circular) (Six-base) MAPSORT of: pFN21AE5520.seq Check: 1941 from: 1 to: 6097
pFN21AE5520 6097 bp
With 182 enzymes: SgfI *
January 30, 2014 17:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3907 278
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3178 3440 1937
Size: 1241 262 4594
Fragments arranged by size:
4594 1241 262
Acc65I G'GTAC_C
Cuts at: 3418 3418
Size: 6097
AclI AA'CG_TT
Cuts at: 1563 4224 4597 1563
Size: 2661 373 3063
Fragments arranged by size:
3063 2661 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4160 5544 5812 1632
Size: 2528 1384 268 1917
Fragments arranged by size:
2528 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5900
AflIII A'CryG_T
Cuts at: 1203 2555 2726 4996 5973 1203
Size: 1352 171 2270 977 1327
Fragments arranged by size:
2270 1352 1327 977 171
AgeI A'CCGG_T
Cuts at: 4928 4928
Size: 6097
AhdI GACnn_n'nnGTC
Cuts at: 4826 4826
Size: 6097
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6097
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6097
AlwNI CAG_nnn'CTG
Cuts at: 2665 3840 5412 2665
Size: 1175 1572 3350
Fragments arranged by size:
3350 1572 1175
ApaI G_GGCC'C
Cuts at: 3212 3212
Size: 6097
ApaLI G'TGCA_C
Cuts at: 4154 5310 4154
Size: 1156 4941
ApoI r'AATT_y
Cuts at: 1493 2460 2533 3016 3360 3406 3632 3784 5893
Size: 967 73 483 344 46 226 152 2109
Cuts at: 5893 1493
Size: 1697
Fragments arranged by size:
2109 1697 967 483 344 226 152 73
46
AseI AT'TA_AT
Cuts at: 160 1032 2153 4651 160
Size: 872 1121 2498 1606
Fragments arranged by size:
2498 1606 1121 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
AvaI C'yCGr_G
Cuts at: 1870 1943 3300 3422 1870
Size: 73 1357 122 4545
Fragments arranged by size:
4545 1357 122 73
BamHI G'GATC_C
Cuts at: 3427 3794 3427
Size: 367 5730
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2417 2623 3418 4873 618
Size: 325 217 627 630 206 795 1455 1842
Fragments arranged by size:
1842 1455 795 630 627 325 217 206
BanII G_rGCy'C
Cuts at: 1391 3212 3416 1391
Size: 1821 204 4072
Fragments arranged by size:
4072 1821 204
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5126
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4288 4322 1903
Size: 34 2351 34 3678
Fragments arranged by size:
3678 2351 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2759 3989 5199 2759
Size: 1230 1210 3657
Fragments arranged by size:
3657 1230 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6097
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4708 136
Size: 107 122 71 631 605 3036 1525
Fragments arranged by size:
3036 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2108 6092 2108
Size: 3984 2113
BlpI GC'TnA_GC
Cuts at: 3514 3514
Size: 6097
Bme1580I G_kGCm'C
Cuts at: 1790 3212 4158 5314 1790
Size: 1422 946 1156 2573
Fragments arranged by size:
2573 1422 1156 946
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6097
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2653 2787 3355
Size: 416 53 83 399 24 1236 134 568
Cuts at: 3355 3828 4786 442
Size: 473 958 1753
Fragments arranged by size:
1753 1236 958 568 473 416 399 134
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6097
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2193 2771 3020 3113 4757 1239
Size: 954 578 249 93 1644 2579
Fragments arranged by size:
2579 1644 954 578 249 93
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2513 4222 5087 5385 2513
Size: 1709 865 298 3225
Fragments arranged by size:
3225 1709 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4760 882
Size: 617 3261 2219
Fragments arranged by size:
3261 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2014 2727 3888 493
Size: 711 810 713 1161 2702
Fragments arranged by size:
2702 1161 810 713 711
BsaBI GATnn'nnATC
Cuts at: 3381 6091 3381
Size: 2710 3387
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3904 4286 4921 275
Size: 53 83 186 936 2371 382 635 1451
Fragments arranged by size:
2371 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4530 4928 5202 5349 5760 4530
Size: 398 274 147 411 4867
Fragments arranged by size:
4867 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5872
Fragments arranged by size:
5872 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6097
BseYI C'CCAG_C
Cuts at: 5300 5796 5300
Size: 496 5601
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2680 1163
Size: 573 944 4580
Fragments arranged by size:
4580 944 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3274 3547 4308 5336 1540
Size: 275 186 1273 273 761 1028 2301
Fragments arranged by size:
2301 1273 1028 761 275 273 186
BsiHKAI G_wGCw'C
Cuts at: 2122 4158 4243 5314 2122
Size: 2036 85 1071 2905
Fragments arranged by size:
2905 2036 1071 85
BsmI GAATG_Cn'
Cuts at: 3608 3701 3608
Size: 93 6004
BsmBI CGTCTCn'nnnn_
Cuts at: 2255 2255
Size: 6097
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2122 3212 3416 4158 4243 5314 1391
Size: 399 332 1090 204 742 85 1071 2174
Fragments arranged by size:
2174 1090 1071 742 399 332 204 85
BspEI T'CCGG_A
Cuts at: 5760 5760
Size: 6097
BspHI T'CATG_A
Cuts at: 2428 3986 5716 2428
Size: 1558 1730 2809
Fragments arranged by size:
2809 1730 1558
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3452 844
Size: 2608 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3984 1394
Size: 429 2161 3507
Fragments arranged by size:
3507 2161 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4592 4766 65
Size: 1183 3344 174 1396
Fragments arranged by size:
3344 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2851 4741 4928 1700
Size: 110 1041 1890 187 2869
Fragments arranged by size:
2869 1890 1041 187 110
BsrGI T'GTAC_A
Cuts at: 96 2548 2784 96
Size: 2452 236 3409
Fragments arranged by size:
3409 2452 236
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6097
BssSI C'ACGA_G
Cuts at: 2913 4157 5169 2913
Size: 1244 1012 3841
Fragments arranged by size:
3841 1244 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6097
BstBI TT'CG_AA
Cuts at: 4901 4901
Size: 6097
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6097
BstYI r'GATC_y
Cuts at: 1972 2108 2524 3427 3794 4179 4196 5648 5734
Size: 136 416 903 367 385 17 1452 86
Cuts at: 5734 5746 6092 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 903 416 385 367 346 136
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2268 2541 513
Size: 552 939 264 273 4069
Fragments arranged by size:
4069 939 552 273 264
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5358
Fragments arranged by size:
5358 683 56
BtsI GCAGTG_nn'
Cuts at: 3613 4407 4427 3613
Size: 794 20 5283
Fragments arranged by size:
5283 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6097
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6062
DraI TTT'AAA
Cuts at: 2200 3401 2200
Size: 1201 4896
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5104 809
Size: 507 3788 1802
Fragments arranged by size:
3788 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2853 3544 4433
Size: 54 1425 50 275 18 1023 691 889
Cuts at: 4433 6062 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1023 889 691 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3544 1537
Size: 275 1732 4090
Fragments arranged by size:
4090 1732 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4027 1353
Size: 2674 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4684 5058 5204 387
Size: 1064 138 3095 374 146 1280
Fragments arranged by size:
3095 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2193 2771 3020 3113 4160 4757 5544
Size: 393 561 578 249 93 1047 597 787
Cuts at: 5544 5812 1239
Size: 268 1524
Fragments arranged by size:
1524 1047 787 597 578 561 393 268
249 93
EcoO109I rG'GnC_Cy
Cuts at: 2204 3208 3209 2204
Size: 1004 1 5092
Fragments arranged by size:
5092 1004 1
EcoRI G'AATT_C
Cuts at: 3406 3784 3406
Size: 378 5719
FspI TGC'GCA
Cuts at: 4603 4603
Size: 6097
HaeII r_GCGC'y
Cuts at: 1678 5244 1678
Size: 3566 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2271 2303 3281 3313 4738
Size: 32 135 32 699 32 978 32 1425
Cuts at: 4738 4770 4812 4844 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1425 978 699 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3441 3687 669
Size: 988 281 1503 246 3079
Fragments arranged by size:
3079 1503 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2575 748
Size: 1827 4270
HpaI GTT'AAC
Cuts at: 3687 3687
Size: 6097
KpnI G_GTAC'C
Cuts at: 3422 3422
Size: 6097
MfeI C'AATT_G
Cuts at: 3696 3696
Size: 6097
MluI A'CGCG_T
Cuts at: 5973 5973
Size: 6097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2352 5211 5395 1305
Size: 1047 2859 184 2007
Fragments arranged by size:
2859 2007 1047 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4275
Fragments arranged by size:
4275 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4055 4414 4573 518
Size: 648 2889 359 159 2042
Fragments arranged by size:
2889 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3011 3513 4190 5338 5583 1606
Size: 1405 502 677 1148 245 2120
Fragments arranged by size:
2120 1405 1148 677 502 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2268 2541 513
Size: 552 939 264 273 4069
Fragments arranged by size:
4069 939 552 273 264
NdeI CA'TA_TG
Cuts at: 387 2938 387
Size: 2551 3546
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6097
NotI GC'GGCC_GC
Cuts at: 3544 3544
Size: 6097
NspI r_CATG'y
Cuts at: 1132 1229 2226 2559 3455 5000 1132
Size: 97 997 333 896 1545 2229
Fragments arranged by size:
2229 1545 997 896 333 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6097
PciI A'CATG_T
Cuts at: 2555 4996 2555
Size: 2441 3656
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6097
PmeI GTTT'AAAC
Cuts at: 3401 3401
Size: 6097
PmlI CAC'GTG
Cuts at: 2014 2014
Size: 6097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4166 4198 5679 5711 4166
Size: 32 1481 32 4552
Fragments arranged by size:
4552 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6097
PsiI TTA'TAA
Cuts at: 3667 3667
Size: 6097
PspOMI G'GGCC_C
Cuts at: 3208 3208
Size: 6097
PstI C_TGCA'G
Cuts at: 830 3449 830
Size: 2619 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6097
PvuII CAG'CTG
Cuts at: 3011 3011
Size: 6097
SalI G'TCGA_C
Cuts at: 1936 3439 1936
Size: 1503 4594
SbfI CC_TGCA'GG
Cuts at: 3449 3449
Size: 6097
ScaI AGT'ACT
Cuts at: 4345 6057 4345
Size: 1712 4385
SfcI C'TryA_G
Cuts at: 826 1045 2439 3445 4580 5261 5452 826
Size: 219 1394 1006 1135 681 191 1471
Fragments arranged by size:
1471 1394 1135 1006 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6097
SmaI CCC'GGG
Cuts at: 1872 3424 1872
Size: 1552 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2528 4201 5102 5364 820
Size: 197 926 585 1673 901 262 1553
Fragments arranged by size:
1673 1553 926 901 585 262 197
SnaBI TAC'GTA
Cuts at: 493 3888 493
Size: 3395 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6097
SphI G_CATG'C
Cuts at: 1132 3455 1132
Size: 2323 3774
SspI AAT'ATT
Cuts at: 5 52 3337 4021 5
Size: 47 3285 684 2081
Fragments arranged by size:
3285 2081 684 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6097
StyI C'CwwG_G
Cuts at: 513 1065 2004 2268 2541 3536 513
Size: 552 939 264 273 995 3074
Fragments arranged by size:
3074 995 939 552 273 264
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4124 4141 4294 4479 4924 325
Size: 1255 2544 17 153 185 445 1498
Fragments arranged by size:
2544 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2548 2784 4343
Size: 274 80 33 51 1443 571 236 1559
Cuts at: 4343 6055 96
Size: 1712 138
Fragments arranged by size:
1712 1559 1443 571 274 236 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6057 6089 6057
Size: 32 6065
Tth111I GACn'n_nGTC
Cuts at: 1316 3174 1316
Size: 1858 4239
XbaI T'CTAG_A
Cuts at: 3433 3433
Size: 6097
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2863 2863
Size: 6097
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6097
XmaI C'CCGG_G
Cuts at: 1870 3422 1870
Size: 1552 4545
XmnI GAAnn'nnTTC
Cuts at: 4226 4226
Size: 6097
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3905 276
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI