Restriction map for
pFN21AE5519
(Circular) (Six-base) MAPSORT of: pFN21AE5519.seq Check: 7128 from: 1 to: 6100
pFN21AE5519 6100 bp
With 182 enzymes: SgfI *
January 30, 2014 17:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3910 278
Size: 53 83 186 936 2374 2468
Fragments arranged by size:
2468 2374 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2537 3443 1937
Size: 600 906 4594
Fragments arranged by size:
4594 906 600
Acc65I G'GTAC_C
Cuts at: 3421 3421
Size: 6100
AclI AA'CG_TT
Cuts at: 1563 4227 4600 1563
Size: 2664 373 3063
Fragments arranged by size:
3063 2664 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2437 2558 4163 5547 5815 1632
Size: 805 121 1605 1384 268 1917
Fragments arranged by size:
1917 1605 1384 805 268 121
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5903
AflIII A'CryG_T
Cuts at: 1203 4999 5976 1203
Size: 3796 977 1327
Fragments arranged by size:
3796 1327 977
AgeI A'CCGG_T
Cuts at: 4931 4931
Size: 6100
AhdI GACnn_n'nnGTC
Cuts at: 3144 4829 3144
Size: 1685 4415
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6100
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6100
AlwNI CAG_nnn'CTG
Cuts at: 2903 3843 5415 2903
Size: 940 1572 3588
Fragments arranged by size:
3588 1572 940
ApaLI G'TGCA_C
Cuts at: 4157 5313 4157
Size: 1156 4944
ApoI r'AATT_y
Cuts at: 1493 2178 2202 2209 2378 2430 2511 2618 3409
Size: 685 24 7 169 52 81 107 791
Cuts at: 3409 3635 3787 5896 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 791 685 226 169 152 107
81 52 24 7
AseI AT'TA_AT
Cuts at: 160 1032 2319 2556 4654 160
Size: 872 1287 237 2098 1606
Fragments arranged by size:
2098 1606 1287 872 237
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6100
AvaI C'yCGr_G
Cuts at: 1870 1943 3425 1870
Size: 73 1482 4545
Fragments arranged by size:
4545 1482 73
BamHI G'GATC_C
Cuts at: 3158 3430 3797 3158
Size: 272 367 5461
Fragments arranged by size:
5461 367 272
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3421 4876 618
Size: 325 217 627 1634 1455 1842
Fragments arranged by size:
1842 1634 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3419 1391
Size: 2028 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2068 3270 928
Size: 971 169 1202 3758
Fragments arranged by size:
3758 1202 971 169
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2895 2929 4291 4325 1903
Size: 34 958 34 1362 34 3678
Fragments arranged by size:
3678 1362 958 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2627 3291 3992 5202 2627
Size: 664 701 1210 3525
Fragments arranged by size:
3525 1210 701 664
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6100
BfrBI ATG'CAT
Cuts at: 2847 2847
Size: 6100
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4711 136
Size: 107 122 71 631 605 3039 1525
Fragments arranged by size:
3039 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6095 6095
Size: 6100
BlpI GC'TnA_GC
Cuts at: 3517 3517
Size: 6100
Bme1580I G_kGCm'C
Cuts at: 1790 4161 5317 1790
Size: 2371 1156 2573
Fragments arranged by size:
2573 2371 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6100
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3831 4789 442
Size: 416 53 83 399 24 2414 958 1753
Fragments arranged by size:
2414 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2722 4760 1239
Size: 1483 2038 2579
Fragments arranged by size:
2579 2038 1483
Bpu10I CC'TnA_GC
Cuts at: 2801 2801
Size: 6100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2446 4225 5090 5388 2446
Size: 1779 865 298 3158
Fragments arranged by size:
3158 1779 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3152 4763 882
Size: 617 1653 1611 2219
Fragments arranged by size:
2219 1653 1611 617
BsaAI yAC'GTr
Cuts at: 493 1204 3891 493
Size: 711 2687 2702
Fragments arranged by size:
2702 2687 711
BsaBI GATnn'nnATC
Cuts at: 6094 6094
Size: 6100
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3907 4289 4924 275
Size: 53 83 186 936 2374 382 635 1451
Fragments arranged by size:
2374 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4533 4931 5205 5352 5763 4533
Size: 398 274 147 411 4870
Fragments arranged by size:
4870 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2041 2071 1178
Size: 30 165 30 638 30 5207
Fragments arranged by size:
5207 638 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6100
BseYI C'CCAG_C
Cuts at: 5303 5799 5303
Size: 496 5604
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5527
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3550 4311 5339 1540
Size: 275 186 1549 761 1028 2301
Fragments arranged by size:
2301 1549 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4161 4246 5317 4161
Size: 85 1071 4944
Fragments arranged by size:
4944 1071 85
BsmI GAATG_Cn'
Cuts at: 2847 3611 3704 2847
Size: 764 93 5243
Fragments arranged by size:
5243 764 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3419 4161 4246 5317 1391
Size: 399 1629 742 85 1071 2174
Fragments arranged by size:
2174 1629 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5763 5763
Size: 6100
BspHI T'CATG_A
Cuts at: 2182 2781 3989 5719 2182
Size: 599 1208 1730 2563
Fragments arranged by size:
2563 1730 1208 599
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3455 844
Size: 2611 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3987 1394
Size: 429 2164 3507
Fragments arranged by size:
3507 2164 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2213 3309 4595 4769 65
Size: 1183 965 1096 1286 174 1396
Fragments arranged by size:
1396 1286 1183 1096 965 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4744 4931 1700
Size: 110 2934 187 2869
Fragments arranged by size:
2934 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6100
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6100
BssSI C'ACGA_G
Cuts at: 4160 5172 4160
Size: 1012 5088
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2903 794
Size: 2109 3991
BstBI TT'CG_AA
Cuts at: 2509 4904 2509
Size: 2395 3705
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6100
BstYI r'GATC_y
Cuts at: 1972 3004 3158 3278 3430 3797 4182 4199 5651
Size: 1032 154 120 152 367 385 17 1452
Cuts at: 5651 5737 5749 6095 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1032 385 367 346 154 152
120 86 17 12
BstZ17I GTA'TAC
Cuts at: 2538 2538
Size: 6100
Bsu36I CC'TnA_GG
Cuts at: 2979 2979
Size: 6100
BtgI C'CryG_G
Cuts at: 513 1065 2004 2592 513
Size: 552 939 588 4021
Fragments arranged by size:
4021 939 588 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3014 487
Size: 683 56 1788 3573
Fragments arranged by size:
3573 1788 683 56
BtsI GCAGTG_nn'
Cuts at: 2904 3616 4410 4430 2904
Size: 712 794 20 4574
Fragments arranged by size:
4574 794 712 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6100
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2254 2289 529
Size: 35 1690 35 4340
Fragments arranged by size:
4340 1690 35 35
DraI TTT'AAA
Cuts at: 2376 3404 2376
Size: 1028 5072
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5107 809
Size: 507 3791 1802
Fragments arranged by size:
3791 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3547 4436 6065
Size: 54 1425 50 275 18 1717 889 1629
Cuts at: 6065 8
Size: 43
Fragments arranged by size:
1717 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3547 1537
Size: 275 1735 4090
Fragments arranged by size:
4090 1735 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2033 4030 1353
Size: 680 1997 3423
Fragments arranged by size:
3423 1997 680
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4687 5061 5207 387
Size: 1064 138 3098 374 146 1280
Fragments arranged by size:
3098 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2437 2558 2722 4163 4760 5547 5815
Size: 393 805 121 164 1441 597 787 268
Cuts at: 5815 1239
Size: 1524
Fragments arranged by size:
1524 1441 805 787 597 393 268 164
121
EcoO109I rG'GnC_Cy
Cuts at: 2009 2009
Size: 6100
EcoRI G'AATT_C
Cuts at: 2209 3409 3787 2209
Size: 1200 378 4522
Fragments arranged by size:
4522 1200 378
EcoRV GAT'ATC
Cuts at: 2194 2194
Size: 6100
FspI TGC'GCA
Cuts at: 4606 4606
Size: 6100
HaeII r_GCGC'y
Cuts at: 1678 5247 1678
Size: 3569 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2476 2508 2695 2727 3130
Size: 32 135 32 904 32 187 32 403
Cuts at: 3130 3162 4741 4773 4815 4847 1373
Size: 32 1579 32 42 32 2626
Fragments arranged by size:
2626 1579 904 403 187 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3444 3690 669
Size: 988 281 1506 246 3079
Fragments arranged by size:
3079 1506 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2019 748
Size: 1271 4829
HpaI GTT'AAC
Cuts at: 3690 3690
Size: 6100
KpnI G_GTAC'C
Cuts at: 3425 3425
Size: 6100
MfeI C'AATT_G
Cuts at: 2958 3699 2958
Size: 741 5359
MluI A'CGCG_T
Cuts at: 5976 5976
Size: 6100
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2404 5214 5398 1305
Size: 1099 2810 184 2007
Fragments arranged by size:
2810 2007 1099 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4278
Fragments arranged by size:
4278 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2396 4058 4417 4576 518
Size: 648 1230 1662 359 159 2042
Fragments arranged by size:
2042 1662 1230 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3516 4193 5341 5586 1606
Size: 1910 677 1148 245 2120
Fragments arranged by size:
2120 1910 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2592 513
Size: 552 939 588 4021
Fragments arranged by size:
4021 939 588 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6100
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6100
NotI GC'GGCC_GC
Cuts at: 3547 3547
Size: 6100
NsiI A_TGCA'T
Cuts at: 2849 2849
Size: 6100
NspI r_CATG'y
Cuts at: 1132 1229 3458 5003 1132
Size: 97 2229 1545 2229
Fragments arranged by size:
2229 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6100
PciI A'CATG_T
Cuts at: 4999 4999
Size: 6100
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6100
PfoI T'CCnGG_A
Cuts at: 3154 3154
Size: 6100
PmeI GTTT'AAAC
Cuts at: 3404 3404
Size: 6100
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4169 4201 5682 5714 4169
Size: 32 1481 32 4555
Fragments arranged by size:
4555 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2009 2009
Size: 6100
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6100
PsiI TTA'TAA
Cuts at: 3670 3670
Size: 6100
PstI C_TGCA'G
Cuts at: 830 3272 3452 830
Size: 2442 180 3478
Fragments arranged by size:
3478 2442 180
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6100
SalI G'TCGA_C
Cuts at: 1936 3442 1936
Size: 1506 4594
SbfI CC_TGCA'GG
Cuts at: 3452 3452
Size: 6100
ScaI AGT'ACT
Cuts at: 4348 6060 4348
Size: 1712 4388
SexAI A'CCwGG_T
Cuts at: 2357 2357
Size: 6100
SfcI C'TryA_G
Cuts at: 826 1045 2928 3268 3448 4583 5264 5455 826
Size: 219 1883 340 180 1135 681 191 1471
Fragments arranged by size:
1883 1471 1135 681 340 219 191 180
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6100
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6100
SmaI CCC'GGG
Cuts at: 1872 3427 1872
Size: 1555 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2461 4204 5105 5367 820
Size: 197 926 518 1743 901 262 1553
Fragments arranged by size:
1743 1553 926 901 518 262 197
SnaBI TAC'GTA
Cuts at: 493 3891 493
Size: 3398 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6100
SphI G_CATG'C
Cuts at: 1132 3458 1132
Size: 2326 3774
SspI AAT'ATT
Cuts at: 5 52 3184 3339 4024 5
Size: 47 3132 155 685 2081
Fragments arranged by size:
3132 2081 685 155 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6100
StyI C'CwwG_G
Cuts at: 513 1065 2004 2592 3539 513
Size: 552 939 588 947 3074
Fragments arranged by size:
3074 947 939 588 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2860 4127 4144 4297 4482 4927 325
Size: 1255 1280 1267 17 153 185 445 1498
Fragments arranged by size:
1498 1280 1267 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2954 4346 6058
Size: 274 80 33 51 1443 977 1392 1712
Cuts at: 6058 96
Size: 138
Fragments arranged by size:
1712 1443 1392 977 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3154 3186 6060 6092 3154
Size: 32 2874 32 3162
Fragments arranged by size:
3162 2874 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6100
XbaI T'CTAG_A
Cuts at: 3332 3436 3332
Size: 104 5996
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6100
XmaI C'CCGG_G
Cuts at: 1870 3425 1870
Size: 1555 4545
XmnI GAAnn'nnTTC
Cuts at: 4229 4229
Size: 6100
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3908 276
Size: 53 83 186 936 2374 2468
Fragments arranged by size:
2468 2374 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI BstXI DraIII EcoICRI EcoNI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI