Restriction map for
pFN21AE5517
(Circular) (Six-base) MAPSORT of: pFN21AE5517.seq Check: 4118 from: 1 to: 6028
pFN21AE5517 6028 bp
With 182 enzymes: SgfI *
January 30, 2014 17:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3838 278
Size: 53 83 186 936 2302 2468
Fragments arranged by size:
2468 2302 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3207 3371 1937
Size: 1270 164 4594
Fragments arranged by size:
4594 1270 164
Acc65I G'GTAC_C
Cuts at: 3349 3349
Size: 6028
AclI AA'CG_TT
Cuts at: 1563 4155 4528 1563
Size: 2592 373 3063
Fragments arranged by size:
3063 2592 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2667 2691 4091 5475 5743 1632
Size: 1035 24 1400 1384 268 1917
Fragments arranged by size:
1917 1400 1384 1035 268 24
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5831
AflIII A'CryG_T
Cuts at: 1203 4927 5904 1203
Size: 3724 977 1327
Fragments arranged by size:
3724 1327 977
AgeI A'CCGG_T
Cuts at: 4859 4859
Size: 6028
AhdI GACnn_n'nnGTC
Cuts at: 4757 4757
Size: 6028
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6028
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6028
AlwNI CAG_nnn'CTG
Cuts at: 3771 5343 3771
Size: 1572 4456
ApaLI G'TGCA_C
Cuts at: 4085 5241 4085
Size: 1156 4872
ApoI r'AATT_y
Cuts at: 1493 2721 3337 3563 3715 5824 1493
Size: 1228 616 226 152 2109 1697
Fragments arranged by size:
2109 1697 1228 616 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4582 160
Size: 872 3550 1606
Fragments arranged by size:
3550 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6028
AvaI C'yCGr_G
Cuts at: 1870 1943 2172 3353 1870
Size: 73 229 1181 4545
Fragments arranged by size:
4545 1181 229 73
AvrII C'CTAG_G
Cuts at: 2315 2315
Size: 6028
BamHI G'GATC_C
Cuts at: 3148 3358 3725 3148
Size: 210 367 5451
Fragments arranged by size:
5451 367 210
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3349 4804 618
Size: 325 217 627 1562 1455 1842
Fragments arranged by size:
1842 1562 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2360 2965 3347 1391
Size: 969 605 382 4072
Fragments arranged by size:
4072 969 605 382
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2679 928
Size: 971 780 4277
Fragments arranged by size:
4277 971 780
BbvCI CC'TCA_GC
Cuts at: 3257 3257
Size: 6028
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4219 4253 1903
Size: 34 50 34 2198 34 3678
Fragments arranged by size:
3678 2198 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3920 5130 3920
Size: 1210 4818
BclI T'GATC_A
Cuts at: 1548 2785 1548
Size: 1237 4791
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2317 3131 4639
Size: 107 122 71 631 605 645 814 1508
Cuts at: 4639 136
Size: 1525
Fragments arranged by size:
1525 1508 814 645 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6023 6023
Size: 6028
BlpI GC'TnA_GC
Cuts at: 3445 3445
Size: 6028
Bme1580I G_kGCm'C
Cuts at: 1790 4089 5245 1790
Size: 2299 1156 2573
Fragments arranged by size:
2573 2299 1156
BmgBI CAC'GTC
Cuts at: 1318 2783 1318
Size: 1465 4563
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2566 3759 4717
Size: 416 53 83 399 24 1149 1193 958
Cuts at: 4717 442
Size: 1753
Fragments arranged by size:
1753 1193 1149 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6028
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2512 2512
Size: 6028
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2093 2358 4688 1239
Size: 854 265 2330 2579
Fragments arranged by size:
2579 2330 854 265
Bpu10I CC'TnA_GC
Cuts at: 3257 3257
Size: 6028
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4153 5018 5316 4153
Size: 865 298 4865
Fragments arranged by size:
4865 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4691 882
Size: 617 3192 2219
Fragments arranged by size:
3192 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3819 493
Size: 711 2615 2702
Fragments arranged by size:
2702 2615 711
BsaBI GATnn'nnATC
Cuts at: 6022 6022
Size: 6028
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3083 3835 4217 4852
Size: 53 83 186 936 1550 752 382 635
Cuts at: 4852 275
Size: 1451
Fragments arranged by size:
1550 1451 936 752 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4461 4859 5133 5280 5691 4461
Size: 398 274 147 411 4798
Fragments arranged by size:
4798 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2877 2907 2983 3013 1178
Size: 30 165 30 1474 30 76 30 4193
Fragments arranged by size:
4193 1474 165 76 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 3021 1186
Size: 847 988 4193
Fragments arranged by size:
4193 988 847
BseYI C'CCAG_C
Cuts at: 5231 5727 5231
Size: 496 5532
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2327 1163
Size: 573 591 4864
Fragments arranged by size:
4864 591 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3478 4239 5267 1540
Size: 275 186 1477 761 1028 2301
Fragments arranged by size:
2301 1477 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2965 4089 4174 5245 2965
Size: 1124 85 1071 3748
Fragments arranged by size:
3748 1124 1071 85
BsmI GAATG_Cn'
Cuts at: 3539 3632 3539
Size: 93 5935
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2360 2965 3347 4089 4174 5245 1391
Size: 399 570 605 382 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 605 570 399 382 85
BspEI T'CCGG_A
Cuts at: 5691 5691
Size: 6028
BspHI T'CATG_A
Cuts at: 3917 5647 3917
Size: 1730 4298
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3383 844
Size: 2539 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3915 1394
Size: 429 2092 3507
Fragments arranged by size:
3507 2092 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4523 4697 65
Size: 1183 3275 174 1396
Fragments arranged by size:
3275 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2065 3268 4672 4859 1700
Size: 110 255 1203 1404 187 2869
Fragments arranged by size:
2869 1404 1203 255 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6028
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6028
BssSI C'ACGA_G
Cuts at: 4088 5100 4088
Size: 1012 5016
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3122 794
Size: 2328 3700
BstBI TT'CG_AA
Cuts at: 4832 4832
Size: 6028
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6028
BstYI r'GATC_y
Cuts at: 1972 2199 3148 3358 3725 4110 4127 5579 5665
Size: 227 949 210 367 385 17 1452 86
Cuts at: 5665 5677 6023 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 949 385 367 346 227 210
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2925 513
Size: 552 1860 3616
Fragments arranged by size:
3616 1860 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5289
Fragments arranged by size:
5289 683 56
BtsI GCAGTG_nn'
Cuts at: 2763 2935 3544 4338 4358 2763
Size: 172 609 794 20 4433
Fragments arranged by size:
4433 794 609 172 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6028
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5993
DraI TTT'AAA
Cuts at: 3091 3332 3091
Size: 241 5787
DraIII CAC_nnn'GTG
Cuts at: 2708 2708
Size: 6028
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5035 809
Size: 507 3719 1802
Fragments arranged by size:
3719 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2127 2391 3006
Size: 54 1425 50 275 18 297 264 615
Cuts at: 3006 3475 4364 5993 8
Size: 469 889 1629 43
Fragments arranged by size:
1629 1425 889 615 469 297 275 264
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3475 1537
Size: 275 1663 4090
Fragments arranged by size:
4090 1663 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2831 3958 1353
Size: 1478 1127 3423
Fragments arranged by size:
3423 1478 1127
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4615 4989 5135 387
Size: 1064 138 3026 374 146 1280
Fragments arranged by size:
3026 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2963 2963
Size: 6028
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2358 2667 2691 4091 4688 5475
Size: 393 461 265 309 24 1400 597 787
Cuts at: 5475 5743 1239
Size: 268 1524
Fragments arranged by size:
1524 1400 787 597 461 393 309 268
265 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2182 2182
Size: 6028
EcoO109I rG'GnC_Cy
Cuts at: 2027 3306 2027
Size: 1279 4749
EcoRI G'AATT_C
Cuts at: 2721 3337 3715 2721
Size: 616 378 5034
Fragments arranged by size:
5034 616 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2218 2218
Size: 6028
FspI TGC'GCA
Cuts at: 4534 4534
Size: 6028
HaeII r_GCGC'y
Cuts at: 1678 5175 1678
Size: 3497 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2068 2100 2347 2379 2877
Size: 32 135 32 496 32 247 32 498
Cuts at: 2877 2909 2983 3015 3140 3172 4669 4701 4743
Size: 32 74 32 125 32 1497 32 42
Cuts at: 4743 4775 1373
Size: 32 2626
Fragments arranged by size:
2626 1497 498 496 247 135 125 74
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3372 3618 669
Size: 988 281 1434 246 3079
Fragments arranged by size:
3079 1434 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3173 748
Size: 2425 3603
HpaI GTT'AAC
Cuts at: 3618 3618
Size: 6028
KpnI G_GTAC'C
Cuts at: 3353 3353
Size: 6028
MfeI C'AATT_G
Cuts at: 3627 3627
Size: 6028
MluI A'CGCG_T
Cuts at: 5904 5904
Size: 6028
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5142 5326 1305
Size: 3837 184 2007
Fragments arranged by size:
3837 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2393 3008 10
Size: 54 1425 343 561 615 3030
Fragments arranged by size:
3030 1425 615 561 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3986 4345 4504 518
Size: 648 2820 359 159 2042
Fragments arranged by size:
2820 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2595 3146 3444 4121 5269 5514 1606
Size: 989 551 298 677 1148 245 2120
Fragments arranged by size:
2120 1148 989 677 551 298 245
NaeI GCC'GGC
Cuts at: 2067 3270 2067
Size: 1203 4825
NcoI C'CATG_G
Cuts at: 513 1065 2925 513
Size: 552 1860 3616
Fragments arranged by size:
3616 1860 552
NdeI CA'TA_TG
Cuts at: 387 3288 387
Size: 2901 3127
NgoMIV G'CCGG_C
Cuts at: 2065 3268 2065
Size: 1203 4825
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6028
NotI GC'GGCC_GC
Cuts at: 3475 3475
Size: 6028
NspI r_CATG'y
Cuts at: 1132 1229 3221 3386 4931 1132
Size: 97 1992 165 1545 2229
Fragments arranged by size:
2229 1992 1545 165 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6028
PciI A'CATG_T
Cuts at: 4927 4927
Size: 6028
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6028
PmeI GTTT'AAAC
Cuts at: 3332 3332
Size: 6028
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4097 4129 5610 5642 4097
Size: 32 1481 32 4483
Fragments arranged by size:
4483 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3306 3306
Size: 6028
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6028
PsiI TTA'TAA
Cuts at: 3598 3598
Size: 6028
PstI C_TGCA'G
Cuts at: 830 3380 830
Size: 2550 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6028
PvuII CAG'CTG
Cuts at: 2595 2595
Size: 6028
SacI G_AGCT'C
Cuts at: 2965 2965
Size: 6028
SalI G'TCGA_C
Cuts at: 1936 3370 1936
Size: 1434 4594
SapI GCTCTTCn'nnn_
Cuts at: 2831 2831
Size: 6028
SbfI CC_TGCA'GG
Cuts at: 3380 3380
Size: 6028
ScaI AGT'ACT
Cuts at: 4276 5988 4276
Size: 1712 4316
SfcI C'TryA_G
Cuts at: 826 1045 2037 3204 3376 4511 5192 5383 826
Size: 219 992 1167 172 1135 681 191 1471
Fragments arranged by size:
1471 1167 1135 992 681 219 191 172
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6028
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6028
SmaI CCC'GGG
Cuts at: 1872 3355 1872
Size: 1483 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2172 4132 5033 5295 820
Size: 197 926 229 1960 901 262 1553
Fragments arranged by size:
1960 1553 926 901 262 229 197
SnaBI TAC'GTA
Cuts at: 493 3819 493
Size: 3326 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6028
SphI G_CATG'C
Cuts at: 1132 3386 1132
Size: 2254 3774
SspI AAT'ATT
Cuts at: 5 52 3095 3952 5
Size: 47 3043 857 2081
Fragments arranged by size:
3043 2081 857 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6028
StyI C'CwwG_G
Cuts at: 513 1065 2315 2925 3049 3309 3467 513
Size: 552 1250 610 124 260 158 3074
Fragments arranged by size:
3074 1250 610 552 260 158 124
SwaI ATTT'AAAT
Cuts at: 3091 3091
Size: 6028
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2977 3144 4055 4072 4225 4410 4855
Size: 1255 1397 167 911 17 153 185 445
Cuts at: 4855 325
Size: 1498
Fragments arranged by size:
1498 1397 1255 911 445 185 167 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4274 5986 96
Size: 274 80 33 51 1443 2297 1712 138
Fragments arranged by size:
2297 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5988 6020 5988
Size: 32 5996
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6028
XbaI T'CTAG_A
Cuts at: 3364 3364
Size: 6028
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6028
XhoI C'TCGA_G
Cuts at: 1943 2172 1943
Size: 229 5799
XmaI C'CCGG_G
Cuts at: 1870 3353 1870
Size: 1483 4545
XmnI GAAnn'nnTTC
Cuts at: 4157 4157
Size: 6028
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3836 276
Size: 53 83 186 936 2302 2468
Fragments arranged by size:
2468 2302 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SrfI