Restriction map for
pFN21AE5515
(Circular) (Six-base) MAPSORT of: pFN21AE5515.seq Check: 1386 from: 1 to: 5785
pFN21AE5515 5785 bp
With 182 enzymes: SgfI *
January 30, 2014 17:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3595 278
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3128 1937
Size: 1191 4594
Acc65I G'GTAC_C
Cuts at: 2372 3106 2372
Size: 734 5051
AclI AA'CG_TT
Cuts at: 1563 3912 4285 1563
Size: 2349 373 3063
Fragments arranged by size:
3063 2349 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3848 5232 5500 1632
Size: 2216 1384 268 1917
Fragments arranged by size:
2216 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5588
AflIII A'CryG_T
Cuts at: 1203 4684 5661 1203
Size: 3481 977 1327
Fragments arranged by size:
3481 1327 977
AgeI A'CCGG_T
Cuts at: 4616 4616
Size: 5785
AhdI GACnn_n'nnGTC
Cuts at: 4514 4514
Size: 5785
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5785
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5785
AlwNI CAG_nnn'CTG
Cuts at: 3528 5100 3528
Size: 1572 4213
ApaLI G'TGCA_C
Cuts at: 3842 4998 3842
Size: 1156 4629
ApoI r'AATT_y
Cuts at: 1493 2953 2975 3094 3320 3472 5581 1493
Size: 1460 22 119 226 152 2109 1697
Fragments arranged by size:
2109 1697 1460 226 152 119 22
AseI AT'TA_AT
Cuts at: 160 1032 4339 160
Size: 872 3307 1606
Fragments arranged by size:
3307 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
AvaI C'yCGr_G
Cuts at: 1870 1943 2916 3110 1870
Size: 73 973 194 4545
Fragments arranged by size:
4545 973 194 73
AvrII C'CTAG_G
Cuts at: 2434 2434
Size: 5785
BamHI G'GATC_C
Cuts at: 3115 3482 3115
Size: 367 5418
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2372 3106 4561 618
Size: 325 217 627 585 734 1455 1842
Fragments arranged by size:
1842 1455 734 627 585 325 217
BanII G_rGCy'C
Cuts at: 1391 3104 1391
Size: 1713 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4814
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3976 4010 1903
Size: 34 2039 34 3678
Fragments arranged by size:
3678 2039 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3677 4887 3677
Size: 1210 4575
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5785
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4396 136
Size: 107 122 71 631 605 2724 1525
Fragments arranged by size:
2724 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3060 5780 3060
Size: 2720 3065
BlpI GC'TnA_GC
Cuts at: 3202 3202
Size: 5785
Bme1580I G_kGCm'C
Cuts at: 1790 2837 3846 5002 1790
Size: 1047 1009 1156 2573
Fragments arranged by size:
2573 1156 1047 1009
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5785
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2379 3516 4474
Size: 416 53 83 399 24 962 1137 958
Cuts at: 4474 442
Size: 1753
Fragments arranged by size:
1753 1137 962 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5785
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2366 2759 2859 4445 1239
Size: 1127 393 100 1586 2579
Fragments arranged by size:
2579 1586 1127 393 100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3910 4775 5073 3910
Size: 865 298 4622
Fragments arranged by size:
4622 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2620 4448 882
Size: 617 1121 1828 2219
Fragments arranged by size:
2219 1828 1121 617
BsaAI yAC'GTr
Cuts at: 493 1204 3576 493
Size: 711 2372 2702
Fragments arranged by size:
2702 2372 711
BsaBI GATnn'nnATC
Cuts at: 5779 5779
Size: 5785
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3592 3974 4609 275
Size: 53 83 186 936 2059 382 635 1451
Fragments arranged by size:
2059 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2055 4218 4616 4890 5037 5448 2055
Size: 2163 398 274 147 411 2392
Fragments arranged by size:
2392 2163 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2415 2445 2723 2753 1178
Size: 30 165 30 1012 30 278 30 4210
Fragments arranged by size:
4210 1012 278 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2025 2976 1186
Size: 839 951 3995
Fragments arranged by size:
3995 951 839
BseYI C'CCAG_C
Cuts at: 4988 5484 4988
Size: 496 5289
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5212
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3235 3996 5024 1540
Size: 275 186 1234 761 1028 2301
Fragments arranged by size:
2301 1234 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2226 3846 3931 5002 2226
Size: 1620 85 1071 3009
Fragments arranged by size:
3009 1620 1071 85
BsmI GAATG_Cn'
Cuts at: 2330 3296 3389 2330
Size: 966 93 4726
Fragments arranged by size:
4726 966 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2226 2837 3104 3846 3931 5002 1391
Size: 399 436 611 267 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 611 436 399 267 85
BspEI T'CCGG_A
Cuts at: 5448 5448
Size: 5785
BspHI T'CATG_A
Cuts at: 2269 3674 5404 2269
Size: 1405 1730 2650
Fragments arranged by size:
2650 1730 1405
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3140 844
Size: 2296 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3672 1394
Size: 429 1849 3507
Fragments arranged by size:
3507 1849 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2829 4280 4454 65
Size: 1183 1581 1451 174 1396
Fragments arranged by size:
1581 1451 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4429 4616 1700
Size: 110 2619 187 2869
Fragments arranged by size:
2869 2619 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5785
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5785
BssSI C'ACGA_G
Cuts at: 3845 4857 3845
Size: 1012 4773
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2019 794
Size: 1225 4560
BstBI TT'CG_AA
Cuts at: 4589 4589
Size: 5785
BstEII G'GTnAC_C
Cuts at: 1184 2065 1184
Size: 881 4904
BstXI CCAn_nnnn'nTGG
Cuts at: 2684 3035 2684
Size: 351 5434
BstYI r'GATC_y
Cuts at: 1972 3060 3115 3482 3867 3884 5336 5422 5434
Size: 1088 55 367 385 17 1452 86 12
Cuts at: 5434 5780 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1088 385 367 346 86 55
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4294
Fragments arranged by size:
4294 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5046
Fragments arranged by size:
5046 683 56
BtsI GCAGTG_nn'
Cuts at: 2226 3301 4095 4115 2226
Size: 1075 794 20 3896
Fragments arranged by size:
3896 1075 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5785
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5750
DraI TTT'AAA
Cuts at: 3089 3089
Size: 5785
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2966 4792 809
Size: 507 1650 1826 1802
Fragments arranged by size:
1826 1802 1650 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2077 2489 2577
Size: 54 1425 50 275 18 247 412 88
Cuts at: 2577 3232 4121 5750 8
Size: 655 889 1629 43
Fragments arranged by size:
1629 1425 889 655 412 275 247 88
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3232 1537
Size: 275 1420 4090
Fragments arranged by size:
4090 1420 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3715 1353
Size: 2362 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4372 4746 4892 387
Size: 1064 138 2783 374 146 1280
Fragments arranged by size:
2783 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2366 2759 2859 3848 4445 5232 5500
Size: 393 734 393 100 989 597 787 268
Cuts at: 5500 1239
Size: 1524
Fragments arranged by size:
1524 989 787 734 597 393 393 268
100
EcoRI G'AATT_C
Cuts at: 3094 3472 3094
Size: 378 5407
EcoRV GAT'ATC
Cuts at: 3025 3025
Size: 5785
FspI TGC'GCA
Cuts at: 4291 4291
Size: 5785
HaeII r_GCGC'y
Cuts at: 1678 2106 4932 1678
Size: 428 2826 2531
Fragments arranged by size:
2826 2531 428
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4426 4458 4500 4532 1373
Size: 32 135 32 2854 32 42 32 2626
Fragments arranged by size:
2854 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2913 3129 3375 669
Size: 988 281 975 216 246 3079
Fragments arranged by size:
3079 988 975 281 246 216
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5785
HpaI GTT'AAC
Cuts at: 3375 3375
Size: 5785
KpnI G_GTAC'C
Cuts at: 2376 3110 2376
Size: 734 5051
MfeI C'AATT_G
Cuts at: 2361 3384 2361
Size: 1023 4762
MluI A'CGCG_T
Cuts at: 5661 5661
Size: 5785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2147 3005 4899 5083 1305
Size: 842 858 1894 184 2007
Fragments arranged by size:
2007 1894 858 842 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2079 2579 10
Size: 54 1425 343 247 500 3216
Fragments arranged by size:
3216 1425 500 343 247 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2426 2547 2682 3033 3743 4102 4261
Size: 648 1260 121 135 351 710 359 159
Cuts at: 4261 518
Size: 2042
Fragments arranged by size:
2042 1260 710 648 359 351 159 135
121
MspA1I CmG'CkG
Cuts at: 1606 2327 2621 3201 3878 5026 5271 1606
Size: 721 294 580 677 1148 245 2120
Fragments arranged by size:
2120 1148 721 677 580 294 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4294
Fragments arranged by size:
4294 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5785
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5785
NotI GC'GGCC_GC
Cuts at: 3232 3232
Size: 5785
NspI r_CATG'y
Cuts at: 1132 1229 2158 3143 4688 1132
Size: 97 929 985 1545 2229
Fragments arranged by size:
2229 1545 985 929 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5785
PciI A'CATG_T
Cuts at: 4684 4684
Size: 5785
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5785
PmeI GTTT'AAAC
Cuts at: 3089 3089
Size: 5785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3854 3886 5367 5399 3854
Size: 32 1481 32 4240
Fragments arranged by size:
4240 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2703 1613
Size: 1090 4695
PsiI TTA'TAA
Cuts at: 3355 3355
Size: 5785
PstI C_TGCA'G
Cuts at: 830 3137 830
Size: 2307 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5785
PvuII CAG'CTG
Cuts at: 2327 2621 2327
Size: 294 5491
SalI G'TCGA_C
Cuts at: 1936 3127 1936
Size: 1191 4594
SbfI CC_TGCA'GG
Cuts at: 3137 3137
Size: 5785
ScaI AGT'ACT
Cuts at: 4033 5745 4033
Size: 1712 4073
SfcI C'TryA_G
Cuts at: 826 1045 2870 3133 4268 4949 5140 826
Size: 219 1825 263 1135 681 191 1471
Fragments arranged by size:
1825 1471 1135 681 263 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5785
SmaI CCC'GGG
Cuts at: 1872 3112 1872
Size: 1240 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3889 4790 5052 820
Size: 197 926 1946 901 262 1553
Fragments arranged by size:
1946 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3576 493
Size: 3083 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5785
SphI G_CATG'C
Cuts at: 1132 3143 1132
Size: 2011 3774
SspI AAT'ATT
Cuts at: 5 52 3709 5
Size: 47 3657 2081
Fragments arranged by size:
3657 2081 47
StuI AGG'CCT
Cuts at: 1517 2439 1517
Size: 922 4863
StyI C'CwwG_G
Cuts at: 513 1065 2004 2070 2434 3224 513
Size: 552 939 66 364 790 3074
Fragments arranged by size:
3074 939 790 552 364 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3812 3829 3982 4167 4612 325
Size: 1255 2232 17 153 185 445 1498
Fragments arranged by size:
2232 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2623 4031 5743
Size: 274 80 33 51 1443 646 1408 1712
Cuts at: 5743 96
Size: 138
Fragments arranged by size:
1712 1443 1408 646 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5745 5777 5745
Size: 32 5753
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5785
XbaI T'CTAG_A
Cuts at: 3121 3121
Size: 5785
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5785
XmaI C'CCGG_G
Cuts at: 1870 3110 1870
Size: 1240 4545
XmnI GAAnn'nnTTC
Cuts at: 3914 3914
Size: 5785
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3593 276
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI