Restriction map for
pFN21AE5514
(Circular) (Six-base) MAPSORT of: pFN21AE5514.seq Check: 9739 from: 1 to: 6070
pFN21AE5514 6070 bp
With 182 enzymes: SgfI *
December 8, 2014 13:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3880 278
Size: 53 83 186 936 2344 2468
Fragments arranged by size:
2468 2344 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3413 1937
Size: 1476 4594
Acc65I G'GTAC_C
Cuts at: 3391 3391
Size: 6070
AclI AA'CG_TT
Cuts at: 1563 4197 4570 1563
Size: 2634 373 3063
Fragments arranged by size:
3063 2634 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2697 3258 4133 5517 5785 1632
Size: 1065 561 875 1384 268 1917
Fragments arranged by size:
1917 1384 1065 875 561 268
AfeI AGC'GCT
Cuts at: 3304 3304
Size: 6070
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5873
AflIII A'CryG_T
Cuts at: 1203 2523 4969 5946 1203
Size: 1320 2446 977 1327
Fragments arranged by size:
2446 1327 1320 977
AgeI A'CCGG_T
Cuts at: 4901 4901
Size: 6070
AhdI GACnn_n'nnGTC
Cuts at: 4799 4799
Size: 6070
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6070
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2173 1184
Size: 989 5081
AlwNI CAG_nnn'CTG
Cuts at: 3813 5385 3813
Size: 1572 4498
ApaLI G'TGCA_C
Cuts at: 4127 5283 4127
Size: 1156 4914
ApoI r'AATT_y
Cuts at: 1493 2904 3379 3605 3757 5866 1493
Size: 1411 475 226 152 2109 1697
Fragments arranged by size:
2109 1697 1411 475 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4624 160
Size: 872 3592 1606
Fragments arranged by size:
3592 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6070
AvaI C'yCGr_G
Cuts at: 1870 1943 3395 1870
Size: 73 1452 4545
Fragments arranged by size:
4545 1452 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2099 2132 2099
Size: 33 6037
BamHI G'GATC_C
Cuts at: 2953 3400 3767 2953
Size: 447 367 5256
Fragments arranged by size:
5256 447 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2272 3346 3364 3391 4846
Size: 325 217 627 485 1074 18 27 1455
Cuts at: 4846 618
Size: 1842
Fragments arranged by size:
1842 1455 1074 627 485 325 217 27
18
BanII G_rGCy'C
Cuts at: 1391 2175 3389 1391
Size: 784 1214 4072
Fragments arranged by size:
4072 1214 784
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5099
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4261 4295 1903
Size: 34 2324 34 3678
Fragments arranged by size:
3678 2324 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3962 5172 3962
Size: 1210 4860
BclI T'GATC_A
Cuts at: 1548 2601 3084 1548
Size: 1053 483 4534
Fragments arranged by size:
4534 1053 483
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4681 136
Size: 107 122 71 631 605 3009 1525
Fragments arranged by size:
3009 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6065 6065
Size: 6070
BlpI GC'TnA_GC
Cuts at: 3487 3487
Size: 6070
Bme1580I G_kGCm'C
Cuts at: 1790 4131 5287 1790
Size: 2341 1156 2573
Fragments arranged by size:
2573 2341 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6070
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3801 4759 442
Size: 416 53 83 399 24 2384 958 1753
Fragments arranged by size:
2384 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6070
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2489 2489
Size: 6070
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2008 4730 1239
Size: 769 2722 2579
Fragments arranged by size:
2722 2579 769
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4195 5060 5358 4195
Size: 865 298 4907
Fragments arranged by size:
4907 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4733 882
Size: 617 3234 2219
Fragments arranged by size:
3234 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3861 493
Size: 711 2657 2702
Fragments arranged by size:
2702 2657 711
BsaBI GATnn'nnATC
Cuts at: 6064 6064
Size: 6070
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3877 4259 4894 275
Size: 53 83 186 936 2344 382 635 1451
Fragments arranged by size:
2344 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4503 4901 5175 5322 5733 4503
Size: 398 274 147 411 4840
Fragments arranged by size:
4840 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3164 3194 1178
Size: 30 165 30 1761 30 4054
Fragments arranged by size:
4054 1761 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3172 1186
Size: 1986 4084
BseYI C'CCAG_C
Cuts at: 5273 5769 5273
Size: 496 5574
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5497
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3520 4281 5309 1540
Size: 275 186 1519 761 1028 2301
Fragments arranged by size:
2301 1519 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2175 4131 4216 5287 2175
Size: 1956 85 1071 2958
Fragments arranged by size:
2958 1956 1071 85
BsmI GAATG_Cn'
Cuts at: 3581 3674 3581
Size: 93 5977
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2175 3389 4131 4216 5287 1391
Size: 399 385 1214 742 85 1071 2174
Fragments arranged by size:
2174 1214 1071 742 399 385 85
BspEI T'CCGG_A
Cuts at: 5733 5733
Size: 6070
BspHI T'CATG_A
Cuts at: 2031 3959 5689 2031
Size: 1928 1730 2412
Fragments arranged by size:
2412 1928 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3425 844
Size: 2581 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3957 1394
Size: 429 2134 3507
Fragments arranged by size:
3507 2134 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2634 4565 4739 65
Size: 1183 1386 1931 174 1396
Fragments arranged by size:
1931 1396 1386 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4714 4901 1700
Size: 110 2904 187 2869
Fragments arranged by size:
2904 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2122 96
Size: 2026 4044
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6070
BssSI C'ACGA_G
Cuts at: 4130 5142 4130
Size: 1012 5058
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6070
BstBI TT'CG_AA
Cuts at: 4874 4874
Size: 6070
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6070
BstYI r'GATC_y
Cuts at: 1972 2389 2421 2953 3400 3767 4152 4169 5621
Size: 417 32 532 447 367 385 17 1452
Cuts at: 5621 5707 5719 6065 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 532 447 417 385 367 346
86 32 17 12
Bsu36I CC'TnA_GG
Cuts at: 2537 2537
Size: 6070
BtgI C'CryG_G
Cuts at: 513 1065 2592 513
Size: 552 1527 3991
Fragments arranged by size:
3991 1527 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5331
Fragments arranged by size:
5331 683 56
BtsI GCAGTG_nn'
Cuts at: 3586 4380 4400 3586
Size: 794 20 5256
Fragments arranged by size:
5256 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6070
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6035
DraI TTT'AAA
Cuts at: 2095 3374 2095
Size: 1279 4791
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5077 809
Size: 507 3761 1802
Fragments arranged by size:
3761 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3517 4406 6035
Size: 54 1425 50 275 18 1687 889 1629
Cuts at: 6035 8
Size: 43
Fragments arranged by size:
1687 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3517 1537
Size: 275 1705 4090
Fragments arranged by size:
4090 1705 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2487 3326 4000 1353
Size: 1134 839 674 3423
Fragments arranged by size:
3423 1134 839 674
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4657 5031 5177 387
Size: 1064 138 3068 374 146 1280
Fragments arranged by size:
3068 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2173 2173
Size: 6070
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2008 2697 3258 4133 4730 5517 5785
Size: 393 376 689 561 875 597 787 268
Cuts at: 5785 1239
Size: 1524
Fragments arranged by size:
1524 875 787 689 597 561 393 376
268
EcoRI G'AATT_C
Cuts at: 3379 3757 3379
Size: 378 5692
FspI TGC'GCA
Cuts at: 4576 4576
Size: 6070
HaeII r_GCGC'y
Cuts at: 1678 3306 5217 1678
Size: 1628 1911 2531
Fragments arranged by size:
2531 1911 1628
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3069 3101 4711 4743 4785
Size: 32 135 32 1497 32 1610 32 42
Cuts at: 4785 4817 1373
Size: 32 2626
Fragments arranged by size:
2626 1610 1497 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2506 3414 3660 669
Size: 988 281 568 908 246 3079
Fragments arranged by size:
3079 988 908 568 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6070
HpaI GTT'AAC
Cuts at: 3660 3660
Size: 6070
KpnI G_GTAC'C
Cuts at: 3395 3395
Size: 6070
MfeI C'AATT_G
Cuts at: 2087 3669 2087
Size: 1582 4488
MluI A'CGCG_T
Cuts at: 5946 5946
Size: 6070
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2335 3028 5184 5368 1305
Size: 1030 693 2156 184 2007
Fragments arranged by size:
2156 2007 1030 693 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4248
Fragments arranged by size:
4248 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2522 2597 4028 4387 4546 518
Size: 648 1356 75 1431 359 159 2042
Fragments arranged by size:
2042 1431 1356 648 359 159 75
MspA1I CmG'CkG
Cuts at: 1606 2801 3486 4163 5311 5556 1606
Size: 1195 685 677 1148 245 2120
Fragments arranged by size:
2120 1195 1148 685 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2592 513
Size: 552 1527 3991
Fragments arranged by size:
3991 1527 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6070
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6070
NotI GC'GGCC_GC
Cuts at: 3517 3517
Size: 6070
NspI r_CATG'y
Cuts at: 1132 1229 2527 3172 3428 4973 1132
Size: 97 1298 645 256 1545 2229
Fragments arranged by size:
2229 1545 1298 645 256 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6070
PciI A'CATG_T
Cuts at: 2523 4969 2523
Size: 2446 3624
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2417 1108
Size: 1309 4761
PmeI GTTT'AAAC
Cuts at: 3374 3374
Size: 6070
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4139 4171 5652 5684 4139
Size: 32 1481 32 4525
Fragments arranged by size:
4525 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6070
PsiI TTA'TAA
Cuts at: 3640 3640
Size: 6070
PstI C_TGCA'G
Cuts at: 830 3258 3422 830
Size: 2428 164 3478
Fragments arranged by size:
3478 2428 164
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6070
PvuII CAG'CTG
Cuts at: 2801 2801
Size: 6070
SacI G_AGCT'C
Cuts at: 2175 2175
Size: 6070
SalI G'TCGA_C
Cuts at: 1936 3412 1936
Size: 1476 4594
SapI GCTCTTCn'nnn_
Cuts at: 2487 2487
Size: 6070
SbfI CC_TGCA'GG
Cuts at: 3422 3422
Size: 6070
ScaI AGT'ACT
Cuts at: 4318 6030 4318
Size: 1712 4358
SfcI C'TryA_G
Cuts at: 826 1045 3254 3418 4553 5234 5425 826
Size: 219 2209 164 1135 681 191 1471
Fragments arranged by size:
2209 1471 1135 681 219 191 164
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6070
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6070
SmaI CCC'GGG
Cuts at: 1872 3397 1872
Size: 1525 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4174 5075 5337 820
Size: 197 926 2231 901 262 1553
Fragments arranged by size:
2231 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3861 493
Size: 3368 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6070
SphI G_CATG'C
Cuts at: 1132 3428 1132
Size: 2296 3774
SspI AAT'ATT
Cuts at: 5 52 2858 2926 3994 5
Size: 47 2806 68 1068 2081
Fragments arranged by size:
2806 2081 1068 68 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6070
StyI C'CwwG_G
Cuts at: 513 1065 2592 2817 3244 3509 513
Size: 552 1527 225 427 265 3074
Fragments arranged by size:
3074 1527 552 427 265 225
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2055 4097 4114 4267 4452 4897 325
Size: 1255 475 2042 17 153 185 445 1498
Fragments arranged by size:
2042 1498 1255 475 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2122 3171 4316
Size: 274 80 33 51 1443 145 1049 1145
Cuts at: 4316 6028 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1145 1049 274 145 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6030 6062 6030
Size: 32 6038
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6070
XbaI T'CTAG_A
Cuts at: 3406 3406
Size: 6070
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2682 2682
Size: 6070
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6070
XmaI C'CCGG_G
Cuts at: 1870 3395 1870
Size: 1525 4545
XmnI GAAnn'nnTTC
Cuts at: 2906 4199 2906
Size: 1293 4777
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3878 276
Size: 53 83 186 936 2344 2468
Fragments arranged by size:
2468 2344 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI