Restriction map for
pFN21AE5512
(Circular) (Six-base) MAPSORT of: pFN21AE5512.seq Check: 7988 from: 1 to: 5785
pFN21AE5512 5785 bp
With 182 enzymes: SgfI *
January 30, 2014 17:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3595 278
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3023 3128 1937
Size: 1086 105 4594
Fragments arranged by size:
4594 1086 105
Acc65I G'GTAC_C
Cuts at: 3106 3106
Size: 5785
AclI AA'CG_TT
Cuts at: 1563 3912 4285 1563
Size: 2349 373 3063
Fragments arranged by size:
3063 2349 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2223 2325 3848 5232 5500 1632
Size: 591 102 1523 1384 268 1917
Fragments arranged by size:
1917 1523 1384 591 268 102
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5588
AflIII A'CryG_T
Cuts at: 1203 4684 5661 1203
Size: 3481 977 1327
Fragments arranged by size:
3481 1327 977
AgeI A'CCGG_T
Cuts at: 4616 4616
Size: 5785
AhdI GACnn_n'nnGTC
Cuts at: 4514 4514
Size: 5785
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5785
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5785
AlwNI CAG_nnn'CTG
Cuts at: 2026 3528 5100 2026
Size: 1502 1572 2711
Fragments arranged by size:
2711 1572 1502
ApaLI G'TGCA_C
Cuts at: 3842 4998 3842
Size: 1156 4629
ApoI r'AATT_y
Cuts at: 1493 2687 3094 3320 3472 5581 1493
Size: 1194 407 226 152 2109 1697
Fragments arranged by size:
2109 1697 1194 407 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4339 160
Size: 872 3307 1606
Fragments arranged by size:
3307 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
AvaI C'yCGr_G
Cuts at: 1870 1943 3110 1870
Size: 73 1167 4545
Fragments arranged by size:
4545 1167 73
BamHI G'GATC_C
Cuts at: 3115 3482 3115
Size: 367 5418
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2913 3106 4561 618
Size: 325 217 627 1126 193 1455 1842
Fragments arranged by size:
1842 1455 1126 627 325 217 193
BanII G_rGCy'C
Cuts at: 1391 2395 3104 1391
Size: 1004 709 4072
Fragments arranged by size:
4072 1004 709
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2200 928
Size: 971 301 4513
Fragments arranged by size:
4513 971 301
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3976 4010 1903
Size: 34 2039 34 3678
Fragments arranged by size:
3678 2039 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3677 4887 3677
Size: 1210 4575
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5785
BfrBI ATG'CAT
Cuts at: 2629 2629
Size: 5785
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4396 136
Size: 107 122 71 631 605 2724 1525
Fragments arranged by size:
2724 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2053 5780 2053
Size: 3727 2058
BlpI GC'TnA_GC
Cuts at: 3202 3202
Size: 5785
Bme1580I G_kGCm'C
Cuts at: 1790 2378 2916 3003 3846 5002 1790
Size: 588 538 87 843 1156 2573
Fragments arranged by size:
2573 1156 843 588 538 87
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5785
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2086 2666 3516
Size: 416 53 83 399 24 669 580 850
Cuts at: 3516 4474 442
Size: 958 1753
Fragments arranged by size:
1753 958 850 669 580 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5785
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2392 2906 4445 1239
Size: 1153 514 1539 2579
Fragments arranged by size:
2579 1539 1153 514
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2034 3910 4775 5073 2034
Size: 1876 865 298 2746
Fragments arranged by size:
2746 1876 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4448 882
Size: 617 2949 2219
Fragments arranged by size:
2949 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3576 493
Size: 711 2372 2702
Fragments arranged by size:
2702 2372 711
BsaBI GATnn'nnATC
Cuts at: 5779 5779
Size: 5785
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3592 3974 4609 275
Size: 53 83 186 936 2059 382 635 1451
Fragments arranged by size:
2059 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4218 4616 4890 5037 5448 4218
Size: 398 274 147 411 4555
Fragments arranged by size:
4555 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3005 3035 1178
Size: 30 165 30 1602 30 3928
Fragments arranged by size:
3928 1602 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5785
BseYI C'CCAG_C
Cuts at: 2909 4988 5484 2909
Size: 2079 496 3210
Fragments arranged by size:
3210 2079 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5212
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3235 3996 5024 1540
Size: 275 186 1234 761 1028 2301
Fragments arranged by size:
2301 1234 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2940 3018 3846 3931 5002 2940
Size: 78 828 85 1071 3723
Fragments arranged by size:
3723 1071 828 85 78
BsmI GAATG_Cn'
Cuts at: 2629 3296 3389 2629
Size: 667 93 5025
Fragments arranged by size:
5025 667 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2378 2395 2916 2940 3003 3018 3104
Size: 399 588 17 521 24 63 15 86
Cuts at: 3104 3846 3931 5002 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 588 521 399 86 85
63 24 17 15
BspEI T'CCGG_A
Cuts at: 5448 5448
Size: 5785
BspHI T'CATG_A
Cuts at: 3674 5404 3674
Size: 1730 4055
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3140 844
Size: 2296 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3672 1394
Size: 429 1849 3507
Fragments arranged by size:
3507 1849 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4280 4454 65
Size: 1183 3032 174 1396
Fragments arranged by size:
3032 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2875 4429 4616 1700
Size: 110 1065 1554 187 2869
Fragments arranged by size:
2869 1554 1065 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5785
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5785
BssSI C'ACGA_G
Cuts at: 3845 4857 3845
Size: 1012 4773
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5785
BstBI TT'CG_AA
Cuts at: 4589 4589
Size: 5785
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5785
BstXI CCAn_nnnn'nTGG
Cuts at: 2170 2197 2170
Size: 27 5758
BstYI r'GATC_y
Cuts at: 1972 2053 3115 3482 3867 3884 5336 5422 5434
Size: 81 1062 367 385 17 1452 86 12
Cuts at: 5434 5780 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1062 385 367 346 86 81
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4294
Fragments arranged by size:
4294 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5046
Fragments arranged by size:
5046 683 56
BtsI GCAGTG_nn'
Cuts at: 3301 4095 4115 3301
Size: 794 20 4971
Fragments arranged by size:
4971 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5785
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5750
DraI TTT'AAA
Cuts at: 2686 3089 2686
Size: 403 5382
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4792 809
Size: 507 3476 1802
Fragments arranged by size:
3476 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3232 4121 5750
Size: 54 1425 50 275 18 1402 889 1629
Cuts at: 5750 8
Size: 43
Fragments arranged by size:
1629 1425 1402 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3232 1537
Size: 275 1420 4090
Fragments arranged by size:
4090 1420 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2004 2783 2939 3715 1353
Size: 651 779 156 776 3423
Fragments arranged by size:
3423 779 776 651 156
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4372 4746 4892 387
Size: 1064 138 2783 374 146 1280
Fragments arranged by size:
2783 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2223 2325 2392 2906 3848 4445 5232
Size: 393 591 102 67 514 942 597 787
Cuts at: 5232 5500 1239
Size: 268 1524
Fragments arranged by size:
1524 942 787 597 591 514 393 268
102 67
EcoO109I rG'GnC_Cy
Cuts at: 2023 2023
Size: 5785
EcoRI G'AATT_C
Cuts at: 3094 3472 3094
Size: 378 5407
FspI TGC'GCA
Cuts at: 4291 4291
Size: 5785
HaeII r_GCGC'y
Cuts at: 1678 2134 4932 1678
Size: 456 2798 2531
Fragments arranged by size:
2798 2531 456
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2382 2414 2726 2758 3005
Size: 32 135 32 810 32 312 32 247
Cuts at: 3005 3037 4426 4458 4500 4532 1373
Size: 32 1389 32 42 32 2626
Fragments arranged by size:
2626 1389 810 312 247 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2452 3129 3375 669
Size: 988 281 514 677 246 3079
Fragments arranged by size:
3079 988 677 514 281 246
HindIII A'AGCT_T
Cuts at: 748 2672 748
Size: 1924 3861
HpaI GTT'AAC
Cuts at: 3375 3375
Size: 5785
KpnI G_GTAC'C
Cuts at: 3110 3110
Size: 5785
MfeI C'AATT_G
Cuts at: 2306 3384 2306
Size: 1078 4707
MluI A'CGCG_T
Cuts at: 5661 5661
Size: 5785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2362 4899 5083 1305
Size: 1057 2537 184 2007
Fragments arranged by size:
2537 2007 1057 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3963
Fragments arranged by size:
3963 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3743 4102 4261 518
Size: 648 2577 359 159 2042
Fragments arranged by size:
2577 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2069 2887 3201 3878 5026 5271 1606
Size: 463 818 314 677 1148 245 2120
Fragments arranged by size:
2120 1148 818 677 463 314 245
NaeI GCC'GGC
Cuts at: 2877 2877
Size: 5785
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4294
Fragments arranged by size:
4294 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5785
NgoMIV G'CCGG_C
Cuts at: 2875 2875
Size: 5785
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5785
NotI GC'GGCC_GC
Cuts at: 3232 3232
Size: 5785
NsiI A_TGCA'T
Cuts at: 2631 2631
Size: 5785
NspI r_CATG'y
Cuts at: 1132 1229 3143 4688 1132
Size: 97 1914 1545 2229
Fragments arranged by size:
2229 1914 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5785
PciI A'CATG_T
Cuts at: 4684 4684
Size: 5785
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5785
PmeI GTTT'AAAC
Cuts at: 3089 3089
Size: 5785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3854 3886 5367 5399 3854
Size: 32 1481 32 4240
Fragments arranged by size:
4240 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5785
PsiI TTA'TAA
Cuts at: 3355 3355
Size: 5785
PstI C_TGCA'G
Cuts at: 830 2157 3137 830
Size: 1327 980 3478
Fragments arranged by size:
3478 1327 980
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5785
PvuII CAG'CTG
Cuts at: 2069 2069
Size: 5785
SalI G'TCGA_C
Cuts at: 1936 3127 1936
Size: 1191 4594
SapI GCTCTTCn'nnn_
Cuts at: 2939 2939
Size: 5785
SbfI CC_TGCA'GG
Cuts at: 3137 3137
Size: 5785
ScaI AGT'ACT
Cuts at: 4033 5745 4033
Size: 1712 4073
SfcI C'TryA_G
Cuts at: 826 1045 2153 2800 3133 4268 4949 5140 826
Size: 219 1108 647 333 1135 681 191 1471
Fragments arranged by size:
1471 1135 1108 681 647 333 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5785
SmaI CCC'GGG
Cuts at: 1872 3112 1872
Size: 1240 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2049 3889 4790 5052 820
Size: 197 926 106 1840 901 262 1553
Fragments arranged by size:
1840 1553 926 901 262 197 106
SnaBI TAC'GTA
Cuts at: 493 3576 493
Size: 3083 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5785
SphI G_CATG'C
Cuts at: 1132 3143 1132
Size: 2011 3774
SspI AAT'ATT
Cuts at: 5 52 3709 5
Size: 47 3657 2081
Fragments arranged by size:
3657 2081 47
StuI AGG'CCT
Cuts at: 1517 2987 1517
Size: 1470 4315
StyI C'CwwG_G
Cuts at: 513 1065 2004 2115 2820 2860 3224 513
Size: 552 939 111 705 40 364 3074
Fragments arranged by size:
3074 939 705 552 364 111 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3812 3829 3982 4167 4612 325
Size: 1255 2232 17 153 185 445 1498
Fragments arranged by size:
2232 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2094 2106 4031
Size: 274 80 33 51 1443 117 12 1925
Cuts at: 4031 5743 96
Size: 1712 138
Fragments arranged by size:
1925 1712 1443 274 138 117 80 51
33 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2212 2244 2555 2587 5745 5777 2212
Size: 32 311 32 3158 32 2220
Fragments arranged by size:
3158 2220 311 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2273 1316
Size: 957 4828
XbaI T'CTAG_A
Cuts at: 2518 3121 2518
Size: 603 5182
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5785
XmaI C'CCGG_G
Cuts at: 1870 3110 1870
Size: 1240 4545
XmnI GAAnn'nnTTC
Cuts at: 3914 3914
Size: 5785
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3593 276
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI