Restriction map for
pFN21AE5511
(Circular) (Six-base) MAPSORT of: pFN21AE5511.seq Check: 5614 from: 1 to: 5929
pFN21AE5511 5929 bp
With 182 enzymes: SgfI *
January 30, 2014 17:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3739 278
Size: 53 83 186 936 2203 2468
Fragments arranged by size:
2468 2203 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3272 1937
Size: 1335 4594
Acc65I G'GTAC_C
Cuts at: 3250 3250
Size: 5929
AclI AA'CG_TT
Cuts at: 1563 4056 4429 1563
Size: 2493 373 3063
Fragments arranged by size:
3063 2493 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2167 2552 3992 5376 5644 1632
Size: 535 385 1440 1384 268 1917
Fragments arranged by size:
1917 1440 1384 535 385 268
AfeI AGC'GCT
Cuts at: 2159 2159
Size: 5929
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5732
AflIII A'CryG_T
Cuts at: 1203 4828 5805 1203
Size: 3625 977 1327
Fragments arranged by size:
3625 1327 977
AgeI A'CCGG_T
Cuts at: 4760 4760
Size: 5929
AhdI GACnn_n'nnGTC
Cuts at: 4658 4658
Size: 5929
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5929
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5929
AlwNI CAG_nnn'CTG
Cuts at: 3672 5244 3672
Size: 1572 4357
ApaI G_GGCC'C
Cuts at: 2033 2515 2033
Size: 482 5447
ApaLI G'TGCA_C
Cuts at: 3986 5142 3986
Size: 1156 4773
ApoI r'AATT_y
Cuts at: 1493 3161 3238 3464 3616 5725 1493
Size: 1668 77 226 152 2109 1697
Fragments arranged by size:
2109 1697 1668 226 152 77
AseI AT'TA_AT
Cuts at: 160 1032 4483 160
Size: 872 3451 1606
Fragments arranged by size:
3451 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5929
AvaI C'yCGr_G
Cuts at: 1870 1943 2032 2250 3150 3254 1870
Size: 73 89 218 900 104 4545
Fragments arranged by size:
4545 900 218 104 89 73
BamHI G'GATC_C
Cuts at: 2871 3259 3626 2871
Size: 388 367 5174
Fragments arranged by size:
5174 388 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2105 2300 2318 3021 3250
Size: 325 217 627 318 195 18 703 229
Cuts at: 3250 4705 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 703 627 325 318 229 217
195 18
BanII G_rGCy'C
Cuts at: 1391 2033 2515 3248 1391
Size: 642 482 733 4072
Fragments arranged by size:
4072 733 642 482
BbeI G_GCGC'C
Cuts at: 2109 2304 3025 2109
Size: 195 721 5013
Fragments arranged by size:
5013 721 195
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2107 2785 928
Size: 971 208 678 4072
Fragments arranged by size:
4072 971 678 208
BbvCI CC'TCA_GC
Cuts at: 2641 2641
Size: 5929
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4120 4154 1903
Size: 34 2183 34 3678
Fragments arranged by size:
3678 2183 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3821 5031 3821
Size: 1210 4719
BclI T'GATC_A
Cuts at: 1548 2451 1548
Size: 903 5026
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2252 2568 3074
Size: 107 122 71 631 605 580 316 506
Cuts at: 3074 3083 4540 136
Size: 9 1457 1525
Fragments arranged by size:
1525 1457 631 605 580 506 316 122
107 71 9
BglII A'GATC_T
Cuts at: 5924 5924
Size: 5929
BlpI GC'TnA_GC
Cuts at: 3346 3346
Size: 5929
Bme1580I G_kGCm'C
Cuts at: 1790 2033 2321 2515 3990 5146 1790
Size: 243 288 194 1475 1156 2573
Fragments arranged by size:
2573 1475 1156 288 243 194
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5929
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3660 4618 442
Size: 416 53 83 399 24 2243 958 1753
Fragments arranged by size:
2243 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5929
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3061 3061
Size: 5929
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2147 2462 2689 4589 1239
Size: 908 315 227 1900 2579
Fragments arranged by size:
2579 1900 908 315 227
Bpu10I CC'TnA_GC
Cuts at: 2201 2641 2201
Size: 440 5489
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4054 4919 5217 4054
Size: 865 298 4766
Fragments arranged by size:
4766 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3206 4592 882
Size: 617 1707 1386 2219
Fragments arranged by size:
2219 1707 1386 617
BsaAI yAC'GTr
Cuts at: 493 1204 3720 493
Size: 711 2516 2702
Fragments arranged by size:
2702 2516 711
BsaBI GATnn'nnATC
Cuts at: 2456 5923 2456
Size: 3467 2462
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2106 2301 3022 3736
Size: 53 83 186 936 573 195 721 714
Cuts at: 3736 4118 4753 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 721 714 635 573 382 195
186 83 53
BsaWI w'CCGG_w
Cuts at: 2499 2961 4362 4760 5034 5181 5592 2499
Size: 462 1401 398 274 147 411 2836
Fragments arranged by size:
2836 1401 462 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5704
Fragments arranged by size:
5704 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2180 2255 1186
Size: 994 75 4860
Fragments arranged by size:
4860 994 75
BseYI C'CCAG_C
Cuts at: 2025 2098 2160 2286 5132 5628 2025
Size: 73 62 126 2846 496 2326
Fragments arranged by size:
2846 2326 496 126 73 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5356
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3379 4140 5168 1540
Size: 275 186 1378 761 1028 2301
Fragments arranged by size:
2301 1378 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2091 3990 4075 5146 2091
Size: 1899 85 1071 2874
Fragments arranged by size:
2874 1899 1071 85
BsmI GAATG_Cn'
Cuts at: 3440 3533 3440
Size: 93 5836
BsmBI CGTCTCn'nnnn_
Cuts at: 2497 2497
Size: 5929
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2033 2091 2321 2515 3248 3990 4075
Size: 399 243 58 230 194 733 742 85
Cuts at: 4075 5146 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 733 399 243 230 194
85 58
BspEI T'CCGG_A
Cuts at: 2499 2961 5592 2499
Size: 462 2631 2836
Fragments arranged by size:
2836 2631 462
BspHI T'CATG_A
Cuts at: 2472 3192 3818 5548 2472
Size: 720 626 1730 2853
Fragments arranged by size:
2853 1730 720 626
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2103 3284 844
Size: 1259 1181 3489
Fragments arranged by size:
3489 1259 1181
BsrBI CCG'CTC
Cuts at: 1394 1823 3816 1394
Size: 429 1993 3507
Fragments arranged by size:
3507 1993 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2912 4424 4598 65
Size: 1183 1664 1512 174 1396
Fragments arranged by size:
1664 1512 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2255 2297 2567 3024 4573 4760 1700
Size: 110 445 42 270 457 1549 187 2869
Fragments arranged by size:
2869 1549 457 445 270 187 110 42
BsrGI T'GTAC_A
Cuts at: 96 2649 96
Size: 2553 3376
BssHII G'CGCG_C
Cuts at: 1925 2057 1925
Size: 132 5797
BssSI C'ACGA_G
Cuts at: 2138 3989 5001 2138
Size: 1851 1012 3066
Fragments arranged by size:
3066 1851 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5929
BstBI TT'CG_AA
Cuts at: 4733 4733
Size: 5929
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5929
BstXI CCAn_nnnn'nTGG
Cuts at: 3145 3145
Size: 5929
BstYI r'GATC_y
Cuts at: 1972 2871 3259 3626 4011 4028 5480 5566 5578
Size: 899 388 367 385 17 1452 86 12
Cuts at: 5578 5924 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 899 388 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2876 2876
Size: 5929
BtgI C'CryG_G
Cuts at: 513 1065 2004 2188 513
Size: 552 939 184 4254
Fragments arranged by size:
4254 939 552 184
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2446 487
Size: 683 56 1220 3970
Fragments arranged by size:
3970 1220 683 56
BtsI GCAGTG_nn'
Cuts at: 2552 3445 4239 4259 2552
Size: 893 794 20 4222
Fragments arranged by size:
4222 893 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5929
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5894
DraI TTT'AAA
Cuts at: 3233 3233
Size: 5929
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4936 809
Size: 507 3620 1802
Fragments arranged by size:
3620 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2011 2073 2175
Size: 54 1425 50 275 18 181 62 102
Cuts at: 2175 2191 2565 3084 3114 3376 4265 5894 8
Size: 16 374 519 30 262 889 1629 43
Fragments arranged by size:
1629 1425 889 519 374 275 262 181
102 62 54 50 43 30 18 16
EagI C'GGCC_G
Cuts at: 1537 1812 3376 1537
Size: 275 1564 4090
Fragments arranged by size:
4090 1564 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2441 3859 1353
Size: 1088 1418 3423
Fragments arranged by size:
3423 1418 1088
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2219 2267 4516 4890 5036 387
Size: 1064 138 630 48 2249 374 146 1280
Fragments arranged by size:
2249 1280 1064 630 374 146 138 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2147 2167 2462 2552 2689 3992 4589
Size: 393 515 20 295 90 137 1303 597
Cuts at: 4589 5376 5644 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1303 787 597 515 393 295 268
137 90 20
EcoNI CCTnn'n_nnAGG
Cuts at: 2045 2519 2045
Size: 474 5455
EcoO109I rG'GnC_Cy
Cuts at: 2029 2511 2512 2728 2029
Size: 482 1 216 5230
Fragments arranged by size:
5230 482 216 1
EcoRI G'AATT_C
Cuts at: 3161 3238 3616 3161
Size: 77 378 5474
Fragments arranged by size:
5474 378 77
FspI TGC'GCA
Cuts at: 4435 4435
Size: 5929
HaeII r_GCGC'y
Cuts at: 1678 2109 2146 2161 2173 2304 2449 3025 5076
Size: 431 37 15 12 131 145 576 2051
Cuts at: 5076 1678
Size: 2531
Fragments arranged by size:
2531 2051 576 431 145 131 37 15
12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2338 2361 2370 2393 4570
Size: 32 135 32 766 23 9 23 2177
Cuts at: 4570 4602 4644 4676 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2177 766 135 42 32 32 32
32 23 23 9
HincII GTy'rAC
Cuts at: 669 1657 1938 3273 3519 669
Size: 988 281 1335 246 3079
Fragments arranged by size:
3079 1335 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5929
HpaI GTT'AAC
Cuts at: 3519 3519
Size: 5929
KasI G'GCGC_C
Cuts at: 2105 2300 3021 2105
Size: 195 721 5013
Fragments arranged by size:
5013 721 195
KpnI G_GTAC'C
Cuts at: 3254 3254
Size: 5929
MfeI C'AATT_G
Cuts at: 3528 3528
Size: 5929
MluI A'CGCG_T
Cuts at: 5805 5805
Size: 5929
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5043 5227 1305
Size: 3738 184 2007
Fragments arranged by size:
3738 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2177 3086 3116 10
Size: 54 1425 343 345 909 30 2823
Fragments arranged by size:
2823 1425 909 345 343 54 30
MslI CAynn'nnrTG
Cuts at: 518 1166 2264 2801 3887 4246 4405 518
Size: 648 1098 537 1086 359 159 2042
Fragments arranged by size:
2042 1098 1086 648 537 359 159
MspA1I CmG'CkG
Cuts at: 1606 2025 2190 2491 2892 2970 3345 4022 5170
Size: 419 165 301 401 78 375 677 1148
Cuts at: 5170 5415 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 419 401 375 301 245
165 78
NaeI GCC'GGC
Cuts at: 2257 2569 3026 2257
Size: 312 457 5160
Fragments arranged by size:
5160 457 312
NarI GG'CG_CC
Cuts at: 2106 2301 3022 2106
Size: 195 721 5013
Fragments arranged by size:
5013 721 195
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4438
Fragments arranged by size:
4438 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5929
NgoMIV G'CCGG_C
Cuts at: 2255 2567 3024 2255
Size: 312 457 5160
Fragments arranged by size:
5160 457 312
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5929
NotI GC'GGCC_GC
Cuts at: 3376 3376
Size: 5929
NspI r_CATG'y
Cuts at: 1132 1229 3287 4832 1132
Size: 97 2058 1545 2229
Fragments arranged by size:
2229 2058 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5929
PciI A'CATG_T
Cuts at: 4828 4828
Size: 5929
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5929
PfoI T'CCnGG_A
Cuts at: 2439 2439
Size: 5929
PmeI GTTT'AAAC
Cuts at: 3233 3233
Size: 5929
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3998 4030 5511 5543 3998
Size: 32 1481 32 4384
Fragments arranged by size:
4384 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2728 2728
Size: 5929
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5929
PsiI TTA'TAA
Cuts at: 3499 3499
Size: 5929
PspOMI G'GGCC_C
Cuts at: 2029 2511 2029
Size: 482 5447
PstI C_TGCA'G
Cuts at: 830 3096 3281 830
Size: 2266 185 3478
Fragments arranged by size:
3478 2266 185
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5929
PvuII CAG'CTG
Cuts at: 2025 2025
Size: 5929
SacII CC_GC'GG
Cuts at: 2191 2191
Size: 5929
SalI G'TCGA_C
Cuts at: 1936 3271 1936
Size: 1335 4594
SbfI CC_TGCA'GG
Cuts at: 3281 3281
Size: 5929
ScaI AGT'ACT
Cuts at: 4177 5889 4177
Size: 1712 4217
SexAI A'CCwGG_T
Cuts at: 2730 2730
Size: 5929
SfcI C'TryA_G
Cuts at: 826 1045 3092 3277 4412 5093 5284 826
Size: 219 2047 185 1135 681 191 1471
Fragments arranged by size:
2047 1471 1135 681 219 191 185
SfiI GGCCn_nnn'nGGCC
Cuts at: 2252 3083 2252
Size: 831 5098
SfoI GGC'GCC
Cuts at: 2107 2302 3023 2107
Size: 195 721 5013
Fragments arranged by size:
5013 721 195
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5929
SgrAI Cr'CCGG_yG
Cuts at: 1700 2297 1700
Size: 597 5332
SmaI CCC'GGG
Cuts at: 1872 2034 3152 3256 1872
Size: 162 1118 104 4545
Fragments arranged by size:
4545 1118 162 104
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4033 4934 5196 820
Size: 197 926 2090 901 262 1553
Fragments arranged by size:
2090 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3720 493
Size: 3227 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5929
SphI G_CATG'C
Cuts at: 1132 3287 1132
Size: 2155 3774
SrfI GCCC'GGGC
Cuts at: 2034 2034
Size: 5929
SspI AAT'ATT
Cuts at: 5 52 3853 5
Size: 47 3801 2081
Fragments arranged by size:
3801 2081 47
StuI AGG'CCT
Cuts at: 1517 2769 3077 1517
Size: 1252 308 4369
Fragments arranged by size:
4369 1252 308
StyI C'CwwG_G
Cuts at: 513 1065 2004 2764 3368 513
Size: 552 939 760 604 3074
Fragments arranged by size:
3074 939 760 604 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2394 2795 3956 3973 4126 4311 4756
Size: 1255 814 401 1161 17 153 185 445
Cuts at: 4756 325
Size: 1498
Fragments arranged by size:
1498 1255 1161 814 445 401 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2586 2649 4175
Size: 274 80 33 51 1443 609 63 1526
Cuts at: 4175 5887 96
Size: 1712 138
Fragments arranged by size:
1712 1526 1443 609 274 138 80 63
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2879 2911 5889 5921 2879
Size: 32 2978 32 2887
Fragments arranged by size:
2978 2887 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2273 2732 1316
Size: 957 459 4513
Fragments arranged by size:
4513 957 459
XbaI T'CTAG_A
Cuts at: 3265 3265
Size: 5929
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5929
XmaI C'CCGG_G
Cuts at: 1870 2032 3150 3254 1870
Size: 162 1118 104 4545
Fragments arranged by size:
4545 1118 162 104
XmnI GAAnn'nnTTC
Cuts at: 4058 4058
Size: 5929
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3737 276
Size: 53 83 186 936 2203 2468
Fragments arranged by size:
2468 2203 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI
PacI PmlI PspXI PsrI RsrII SacI SanDI SapI
SwaI XcmI