Restriction map for
pFN21AE5509
(Circular) (Six-base) MAPSORT of: pFN21AE5509.seq Check: 1135 from: 1 to: 5995
pFN21AE5509 5995 bp
With 182 enzymes: SgfI *
January 30, 2014 17:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3216 3805 278
Size: 53 83 186 936 1680 589 2468
Fragments arranged by size:
2468 1680 936 589 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3338 1937
Size: 1401 4594
Acc65I G'GTAC_C
Cuts at: 3316 3316
Size: 5995
AclI AA'CG_TT
Cuts at: 1563 2685 4122 4495 1563
Size: 1122 1437 373 3063
Fragments arranged by size:
3063 1437 1122 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4058 5442 5710 1632
Size: 2426 1384 268 1917
Fragments arranged by size:
2426 1917 1384 268
AfeI AGC'GCT
Cuts at: 3260 3260
Size: 5995
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5798
AflIII A'CryG_T
Cuts at: 1203 2997 4894 5871 1203
Size: 1794 1897 977 1327
Fragments arranged by size:
1897 1794 1327 977
AgeI A'CCGG_T
Cuts at: 4826 4826
Size: 5995
AhdI GACnn_n'nnGTC
Cuts at: 2061 2438 4724 2061
Size: 377 2286 3332
Fragments arranged by size:
3332 2286 377
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5995
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2490 1184
Size: 1306 4689
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2068 2100 2068
Size: 32 5963
AlwNI CAG_nnn'CTG
Cuts at: 2321 3738 5310 2321
Size: 1417 1572 3006
Fragments arranged by size:
3006 1572 1417
ApaI G_GGCC'C
Cuts at: 3174 3174
Size: 5995
ApaLI G'TGCA_C
Cuts at: 2199 4052 5208 2199
Size: 1853 1156 2986
Fragments arranged by size:
2986 1853 1156
ApoI r'AATT_y
Cuts at: 1493 3304 3530 3682 5791 1493
Size: 1811 226 152 2109 1697
Fragments arranged by size:
2109 1811 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3024 4549 160
Size: 872 1992 1525 1606
Fragments arranged by size:
1992 1606 1525 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5995
AvaI C'yCGr_G
Cuts at: 1870 1943 3165 3320 1870
Size: 73 1222 155 4545
Fragments arranged by size:
4545 1222 155 73
BamHI G'GATC_C
Cuts at: 3184 3325 3692 3184
Size: 141 367 5487
Fragments arranged by size:
5487 367 141
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3316 4771 618
Size: 325 217 627 1529 1455 1842
Fragments arranged by size:
1842 1529 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3063 3174 3314 1391
Size: 1672 111 140 4072
Fragments arranged by size:
4072 1672 140 111
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2868 3245 928
Size: 971 969 377 3678
Fragments arranged by size:
3678 971 969 377
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1999 2033 4186 4220 1903
Size: 34 62 34 2153 34 3678
Fragments arranged by size:
3678 2153 62 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2661 3887 5097 2661
Size: 1226 1210 3559
Fragments arranged by size:
3559 1226 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5995
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4606 136
Size: 107 122 71 631 605 2934 1525
Fragments arranged by size:
2934 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5990 5990
Size: 5995
BlpI GC'TnA_GC
Cuts at: 3032 3412 3032
Size: 380 5615
Bme1580I G_kGCm'C
Cuts at: 1790 2203 3174 4056 5212 1790
Size: 413 971 882 1156 2573
Fragments arranged by size:
2573 1156 971 882 413
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5995
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2168 2226 3235
Size: 416 53 83 399 24 751 58 1009
Cuts at: 3235 3726 4684 442
Size: 491 958 1753
Fragments arranged by size:
1753 1009 958 751 491 416 399 83
58 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5995
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2349 3049 4655 1239
Size: 1110 700 1606 2579
Fragments arranged by size:
2579 1606 1110 700
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2212 2693 3196 4120 4985 5283 2212
Size: 481 503 924 865 298 2924
Fragments arranged by size:
2924 924 865 503 481 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3016 4658 882
Size: 617 1517 1642 2219
Fragments arranged by size:
2219 1642 1517 617
BsaAI yAC'GTr
Cuts at: 493 1204 3786 493
Size: 711 2582 2702
Fragments arranged by size:
2702 2582 711
BsaBI GATnn'nnATC
Cuts at: 5989 5989
Size: 5995
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3213 3802 4184 4819
Size: 53 83 186 936 1680 589 382 635
Cuts at: 4819 275
Size: 1451
Fragments arranged by size:
1680 1451 936 635 589 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4428 4826 5100 5247 5658 4428
Size: 398 274 147 411 4765
Fragments arranged by size:
4765 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2068 2098 1178
Size: 30 165 30 665 30 5075
Fragments arranged by size:
5075 665 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5995
BseYI C'CCAG_C
Cuts at: 5198 5694 5198
Size: 496 5499
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2486 3228 1163
Size: 573 750 742 3930
Fragments arranged by size:
3930 750 742 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2013 3445 4206 5234 1540
Size: 275 186 12 1432 761 1028 2301
Fragments arranged by size:
2301 1432 1028 761 275 186 12
BsiHKAI G_wGCw'C
Cuts at: 2203 4056 4141 5212 2203
Size: 1853 85 1071 2986
Fragments arranged by size:
2986 1853 1071 85
BsmI GAATG_Cn'
Cuts at: 3506 3599 3506
Size: 93 5902
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2203 3063 3174 3314 4056 4141 5212
Size: 399 413 860 111 140 742 85 1071
Cuts at: 5212 1391
Size: 2174
Fragments arranged by size:
2174 1071 860 742 413 399 140 111
85
BspEI T'CCGG_A
Cuts at: 5658 5658
Size: 5995
BspHI T'CATG_A
Cuts at: 3884 5614 3884
Size: 1730 4265
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2051 2756 3350 844
Size: 1207 705 594 3489
Fragments arranged by size:
3489 1207 705 594
BsrBI CCG'CTC
Cuts at: 1394 1823 3882 1394
Size: 429 2059 3507
Fragments arranged by size:
3507 2059 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2146 2472 4490 4664 65
Size: 1183 898 326 2018 174 1396
Fragments arranged by size:
2018 1396 1183 898 326 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2976 4639 4826 1700
Size: 110 1166 1663 187 2869
Fragments arranged by size:
2869 1663 1166 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5995
BssHII G'CGCG_C
Cuts at: 1925 2020 1925
Size: 95 5900
BssSI C'ACGA_G
Cuts at: 4055 5067 4055
Size: 1012 4983
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5995
BstBI TT'CG_AA
Cuts at: 4799 4799
Size: 5995
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5995
BstYI r'GATC_y
Cuts at: 1972 2377 3184 3325 3692 4077 4094 5546 5632
Size: 405 807 141 367 385 17 1452 86
Cuts at: 5632 5644 5990 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 807 405 385 367 346 141
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2013 3055 3291 513
Size: 552 939 9 1042 236 3217
Fragments arranged by size:
3217 1042 939 552 236 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5256
Fragments arranged by size:
5256 683 56
BtsI GCAGTG_nn'
Cuts at: 2087 2201 2469 3511 4305 4325 2087
Size: 114 268 1042 794 20 3757
Fragments arranged by size:
3757 1042 794 268 114 20
ClaI AT'CG_AT
Cuts at: 1554 2723 1554
Size: 1169 4826
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5960
DraI TTT'AAA
Cuts at: 2899 3299 2899
Size: 400 5595
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5002 809
Size: 507 3686 1802
Fragments arranged by size:
3686 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2143 3442 4331
Size: 54 1425 50 275 18 313 1299 889
Cuts at: 4331 5960 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1299 889 313 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3442 1537
Size: 275 1630 4090
Fragments arranged by size:
4090 1630 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2120 2338 2487 2529 3056 3275 3925 1353
Size: 767 218 149 42 527 219 650 3423
Fragments arranged by size:
3423 767 650 527 219 218 149 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4582 4956 5102 387
Size: 1064 138 2993 374 146 1280
Fragments arranged by size:
2993 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2349 3049 4058 4655 5442 5710 1239
Size: 393 717 700 1009 597 787 268 1524
Fragments arranged by size:
1524 1009 787 717 700 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3041 3041
Size: 5995
EcoO109I rG'GnC_Cy
Cuts at: 3046 3103 3170 3171 3046
Size: 57 67 1 5870
Fragments arranged by size:
5870 67 57 1
EcoRI G'AATT_C
Cuts at: 3304 3682 3304
Size: 378 5617
FspI TGC'GCA
Cuts at: 4501 4501
Size: 5995
HaeII r_GCGC'y
Cuts at: 1678 2310 3262 5142 1678
Size: 632 952 1880 2531
Fragments arranged by size:
2531 1880 952 632
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2047 2079 2424 2456 3169
Size: 32 135 32 475 32 345 32 713
Cuts at: 3169 3201 4636 4668 4710 4742 1373
Size: 32 1435 32 42 32 2626
Fragments arranged by size:
2626 1435 713 475 345 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3339 3585 669
Size: 988 281 1401 246 3079
Fragments arranged by size:
3079 1401 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5995
HpaI GTT'AAC
Cuts at: 3585 3585
Size: 5995
KpnI G_GTAC'C
Cuts at: 3320 3320
Size: 5995
MfeI C'AATT_G
Cuts at: 3594 3594
Size: 5995
MluI A'CGCG_T
Cuts at: 5871 5871
Size: 5995
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5109 5293 1305
Size: 3804 184 2007
Fragments arranged by size:
3804 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2145 10
Size: 54 1425 343 313 3860
Fragments arranged by size:
3860 1425 343 313 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3026 3953 4312 4471 518
Size: 648 1860 927 359 159 2042
Fragments arranged by size:
2042 1860 927 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2015 2949 3088 3411 4088 5236 5481 1606
Size: 409 934 139 323 677 1148 245 2120
Fragments arranged by size:
2120 1148 934 677 409 323 245 139
NaeI GCC'GGC
Cuts at: 2978 2978
Size: 5995
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4504
Fragments arranged by size:
4504 939 552
NdeI CA'TA_TG
Cuts at: 387 2647 387
Size: 2260 3735
NgoMIV G'CCGG_C
Cuts at: 2976 2976
Size: 5995
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5995
NotI GC'GGCC_GC
Cuts at: 3442 3442
Size: 5995
NspI r_CATG'y
Cuts at: 1132 1229 3001 3353 4898 1132
Size: 97 1772 352 1545 2229
Fragments arranged by size:
2229 1772 1545 352 97
PasI CC'CwG_GG
Cuts at: 1348 3043 1348
Size: 1695 4300
PciI A'CATG_T
Cuts at: 2997 4894 2997
Size: 1897 4098
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3115 1108
Size: 2007 3988
PfoI T'CCnGG_A
Cuts at: 3123 3123
Size: 5995
PmeI GTTT'AAAC
Cuts at: 3299 3299
Size: 5995
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2068 2100 4064 4096 5577 5609 2068
Size: 32 1964 32 1481 32 2454
Fragments arranged by size:
2454 1964 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2628 1613
Size: 1015 4980
PsiI TTA'TAA
Cuts at: 3565 3565
Size: 5995
PspOMI G'GGCC_C
Cuts at: 3170 3170
Size: 5995
PstI C_TGCA'G
Cuts at: 830 2208 2290 2767 3347 830
Size: 1378 82 477 580 3478
Fragments arranged by size:
3478 1378 580 477 82
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5995
PvuII CAG'CTG
Cuts at: 3088 3088
Size: 5995
SacII CC_GC'GG
Cuts at: 2016 2016
Size: 5995
SalI G'TCGA_C
Cuts at: 1936 3337 1936
Size: 1401 4594
SapI GCTCTTCn'nnn_
Cuts at: 2487 2487
Size: 5995
SbfI CC_TGCA'GG
Cuts at: 3347 3347
Size: 5995
ScaI AGT'ACT
Cuts at: 4243 5955 4243
Size: 1712 4283
SfcI C'TryA_G
Cuts at: 826 1045 2204 2286 2763 3343 4478 5159 5350
Size: 219 1159 82 477 580 1135 681 191
Cuts at: 5350 826
Size: 1471
Fragments arranged by size:
1471 1159 1135 681 580 477 219 191
82
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5995
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5995
SmaI CCC'GGG
Cuts at: 1872 3167 3322 1872
Size: 1295 155 4545
Fragments arranged by size:
4545 1295 155
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2191 2672 3175 4099 5000 5262
Size: 197 926 248 481 503 924 901 262
Cuts at: 5262 820
Size: 1553
Fragments arranged by size:
1553 926 924 901 503 481 262 248
197
SnaBI TAC'GTA
Cuts at: 493 3786 493
Size: 3293 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5995
SphI G_CATG'C
Cuts at: 1132 3353 1132
Size: 2221 3774
SspI AAT'ATT
Cuts at: 5 52 2874 3919 5
Size: 47 2822 1045 2081
Fragments arranged by size:
2822 2081 1045 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5995
StyI C'CwwG_G
Cuts at: 513 1065 2004 3006 3434 513
Size: 552 939 1002 428 3074
Fragments arranged by size:
3074 1002 939 552 428
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2189 2559 3056 4022 4039 4192 4377
Size: 1255 609 370 497 966 17 153 185
Cuts at: 4377 4822 325
Size: 445 1498
Fragments arranged by size:
1498 1255 966 609 497 445 370 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4241 5953 96
Size: 274 80 33 51 1443 2264 1712 138
Fragments arranged by size:
2264 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5955 5987 5955
Size: 32 5963
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5995
XbaI T'CTAG_A
Cuts at: 3331 3331
Size: 5995
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5995
XmaI C'CCGG_G
Cuts at: 1870 3165 3320 1870
Size: 1295 155 4545
Fragments arranged by size:
4545 1295 155
XmnI GAAnn'nnTTC
Cuts at: 4124 4124
Size: 5995
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3214 3803 276
Size: 53 83 186 936 1680 589 2468
Fragments arranged by size:
2468 1680 936 589 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PmlI PpuMI PspXI PsrI RsrII SacI SanDI
SexAI SfiI SfoI SrfI SwaI XcmI