Restriction map for
pFN21AE5508
(Circular) (Six-base) MAPSORT of: pFN21AE5508.seq Check: 6292 from: 1 to: 6016
pFN21AE5508 6016 bp
With 182 enzymes: SgfI *
January 30, 2014 17:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2053 3090 2053
Size: 1037 4979
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3826 278
Size: 53 83 186 936 2290 2468
Fragments arranged by size:
2468 2290 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3359 1937
Size: 1422 4594
Acc65I G'GTAC_C
Cuts at: 3337 3337
Size: 6016
AclI AA'CG_TT
Cuts at: 1563 4143 4516 1563
Size: 2580 373 3063
Fragments arranged by size:
3063 2580 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2790 4079 5463 5731 1632
Size: 1158 1289 1384 268 1917
Fragments arranged by size:
1917 1384 1289 1158 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5819
AflIII A'CryG_T
Cuts at: 1203 4915 5892 1203
Size: 3712 977 1327
Fragments arranged by size:
3712 1327 977
AgeI A'CCGG_T
Cuts at: 4847 4847
Size: 6016
AhdI GACnn_n'nnGTC
Cuts at: 4745 4745
Size: 6016
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6016
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6016
AlwNI CAG_nnn'CTG
Cuts at: 2404 3759 5331 2404
Size: 1355 1572 3089
Fragments arranged by size:
3089 1572 1355
ApaI G_GGCC'C
Cuts at: 2072 2997 2072
Size: 925 5091
ApaLI G'TGCA_C
Cuts at: 4073 5229 4073
Size: 1156 4860
ApoI r'AATT_y
Cuts at: 1493 2332 3325 3551 3703 5812 1493
Size: 839 993 226 152 2109 1697
Fragments arranged by size:
2109 1697 993 839 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4570 160
Size: 872 3538 1606
Fragments arranged by size:
3538 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6016
AvaI C'yCGr_G
Cuts at: 1870 1943 2337 3341 1870
Size: 73 394 1004 4545
Fragments arranged by size:
4545 1004 394 73
BamHI G'GATC_C
Cuts at: 3346 3713 3346
Size: 367 5649
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2828 3094 3175 3337 4792
Size: 325 217 627 1041 266 81 162 1455
Cuts at: 4792 618
Size: 1842
Fragments arranged by size:
1842 1455 1041 627 325 266 217 162
81
BanII G_rGCy'C
Cuts at: 1391 2072 2207 2368 2556 2754 2997 3335 1391
Size: 681 135 161 188 198 243 338 4072
Fragments arranged by size:
4072 681 338 243 198 188 161 135
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3122 928
Size: 971 1223 3822
Fragments arranged by size:
3822 1223 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2429 2463 4207 4241 1903
Size: 34 492 34 1744 34 3678
Fragments arranged by size:
3678 1744 492 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3908 5118 3908
Size: 1210 4806
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6016
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4627 136
Size: 107 122 71 631 605 2955 1525
Fragments arranged by size:
2955 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2916 3042 6011 2916
Size: 126 2969 2921
Fragments arranged by size:
2969 2921 126
BlpI GC'TnA_GC
Cuts at: 2648 3433 2648
Size: 785 5231
Bme1580I G_kGCm'C
Cuts at: 1790 2072 2997 3180 4077 5233 1790
Size: 282 925 183 897 1156 2573
Fragments arranged by size:
2573 1156 925 897 282 183
BmgBI CAC'GTC
Cuts at: 1318 2950 1318
Size: 1632 4384
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2414 2650 3747
Size: 416 53 83 399 24 997 236 1097
Cuts at: 3747 4705 442
Size: 958 1753
Fragments arranged by size:
1753 1097 997 958 416 399 236 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6016
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2372 3086 2372
Size: 714 5302
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2570 2768 4676 1239
Size: 1331 198 1908 2579
Fragments arranged by size:
2579 1908 1331 198
Bpu10I CC'TnA_GC
Cuts at: 2663 2936 2663
Size: 273 5743
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2780 3296 4141 5006 5304 2780
Size: 516 845 865 298 3492
Fragments arranged by size:
3492 865 845 516 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2477 4679 882
Size: 617 978 2202 2219
Fragments arranged by size:
2219 2202 978 617
BsaAI yAC'GTr
Cuts at: 493 1204 3223 3807 493
Size: 711 2019 584 2702
Fragments arranged by size:
2702 2019 711 584
BsaBI GATnn'nnATC
Cuts at: 6010 6010
Size: 6016
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3823 4205 4840 275
Size: 53 83 186 936 2290 382 635 1451
Fragments arranged by size:
2290 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4449 4847 5121 5268 5679 4449
Size: 398 274 147 411 4786
Fragments arranged by size:
4786 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3212 3242 1178
Size: 30 165 30 1809 30 3952
Fragments arranged by size:
3952 1809 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3299 1186
Size: 2113 3903
BseYI C'CCAG_C
Cuts at: 2015 2840 2845 2985 5219 5715 2015
Size: 825 5 140 2234 496 2316
Fragments arranged by size:
2316 2234 825 496 140 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3033 1163
Size: 573 1297 4146
Fragments arranged by size:
4146 1297 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3466 4227 5255 1540
Size: 275 186 1465 761 1028 2301
Fragments arranged by size:
2301 1465 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2556 2754 4077 4162 5233 2556
Size: 198 1323 85 1071 3339
Fragments arranged by size:
3339 1323 1071 198 85
BsmI GAATG_Cn'
Cuts at: 2377 3527 3620 2377
Size: 1150 93 4773
Fragments arranged by size:
4773 1150 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2207 2368 2556 2754 2997 3180
Size: 399 282 135 161 188 198 243 183
Cuts at: 3180 3335 4077 4162 5233 1391
Size: 155 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 282 243 198 188
183 161 155 135 85
BspEI T'CCGG_A
Cuts at: 5679 5679
Size: 6016
BspHI T'CATG_A
Cuts at: 2706 3905 5635 2706
Size: 1199 1730 3087
Fragments arranged by size:
3087 1730 1199
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2053 3090 3371 844
Size: 1209 1037 281 3489
Fragments arranged by size:
3489 1209 1037 281
BsrBI CCG'CTC
Cuts at: 1394 1823 2089 3903 1394
Size: 429 266 1814 3507
Fragments arranged by size:
3507 1814 429 266
BsrDI GCAATG_nn'
Cuts at: 65 1248 2833 4511 4685 65
Size: 1183 1585 1678 174 1396
Fragments arranged by size:
1678 1585 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4660 4847 1700
Size: 110 2850 187 2869
Fragments arranged by size:
2869 2850 187 110
BsrGI T'GTAC_A
Cuts at: 96 3106 96
Size: 3010 3006
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6016
BssSI C'ACGA_G
Cuts at: 4076 5088 4076
Size: 1012 5004
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2835 794
Size: 2041 3975
BstBI TT'CG_AA
Cuts at: 4820 4820
Size: 6016
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6016
BstXI CCAn_nnnn'nTGG
Cuts at: 2997 2997
Size: 6016
BstYI r'GATC_y
Cuts at: 1972 2569 2688 2916 3042 3346 3713 4098 4115
Size: 597 119 228 126 304 367 385 17
Cuts at: 4115 5567 5653 5665 6011 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 597 385 367 346 304 228
126 119 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2268 2573 513
Size: 552 939 264 305 3956
Fragments arranged by size:
3956 939 552 305 264
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3294 487
Size: 683 56 2068 3209
Fragments arranged by size:
3209 2068 683 56
BtsI GCAGTG_nn'
Cuts at: 3129 3532 4326 4346 3129
Size: 403 794 20 4799
Fragments arranged by size:
4799 794 403 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6016
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5981
DraI TTT'AAA
Cuts at: 3320 3320
Size: 6016
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5023 809
Size: 507 3707 1802
Fragments arranged by size:
3707 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2197 3463 4352
Size: 54 1425 50 275 18 367 1266 889
Cuts at: 4352 5981 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1266 889 367 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3463 1537
Size: 275 1651 4090
Fragments arranged by size:
4090 1651 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2531 2573 3033 3946 1353
Size: 1178 42 460 913 3423
Fragments arranged by size:
3423 1178 913 460 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3281 4603 4977 5123 387
Size: 1064 138 1692 1322 374 146 1280
Fragments arranged by size:
1692 1322 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2554 2752 2554
Size: 198 5818
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2570 2768 2790 4079 4676 5463 5731
Size: 393 938 198 22 1289 597 787 268
Cuts at: 5731 1239
Size: 1524
Fragments arranged by size:
1524 1289 938 787 597 393 268 198
22
EcoNI CCTnn'n_nnAGG
Cuts at: 2939 3086 2939
Size: 147 5869
EcoO109I rG'GnC_Cy
Cuts at: 2069 2341 2857 2993 2994 2069
Size: 272 516 136 1 5091
Fragments arranged by size:
5091 516 272 136 1
EcoRI G'AATT_C
Cuts at: 2332 3325 3703 2332
Size: 993 378 4645
Fragments arranged by size:
4645 993 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2935 2935
Size: 6016
FspI TGC'GCA
Cuts at: 4522 4522
Size: 6016
HaeII r_GCGC'y
Cuts at: 1678 5163 1678
Size: 3485 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4657 4689 4731 4763 1373
Size: 32 135 32 3085 32 42 32 2626
Fragments arranged by size:
3085 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2385 3286 3360 3606 669
Size: 988 281 447 901 74 246 3079
Fragments arranged by size:
3079 988 901 447 281 246 74
HindIII A'AGCT_T
Cuts at: 748 3167 748
Size: 2419 3597
HpaI GTT'AAC
Cuts at: 3606 3606
Size: 6016
KpnI G_GTAC'C
Cuts at: 3341 3341
Size: 6016
MfeI C'AATT_G
Cuts at: 3615 3615
Size: 6016
MluI A'CGCG_T
Cuts at: 5892 5892
Size: 6016
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2146 2418 2544 3184 5130 5314 1305
Size: 841 272 126 640 1946 184 2007
Fragments arranged by size:
2007 1946 841 640 272 184 126
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2199 10
Size: 54 1425 343 367 3827
Fragments arranged by size:
3827 1425 367 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3974 4333 4492 518
Size: 648 2808 359 159 2042
Fragments arranged by size:
2808 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2019 2270 3432 4109 5257 5502 1606
Size: 413 251 1162 677 1148 245 2120
Fragments arranged by size:
2120 1162 1148 677 413 251 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2573 513
Size: 552 939 569 3956
Fragments arranged by size:
3956 939 569 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6016
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6016
NotI GC'GGCC_GC
Cuts at: 3463 3463
Size: 6016
NruI TCG'CGA
Cuts at: 2081 2081
Size: 6016
NspI r_CATG'y
Cuts at: 1132 1229 3374 4919 1132
Size: 97 2145 1545 2229
Fragments arranged by size:
2229 2145 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2997 1348
Size: 1649 4367
PciI A'CATG_T
Cuts at: 4915 4915
Size: 6016
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2404 2461 1108
Size: 1296 57 4663
Fragments arranged by size:
4663 1296 57
PfoI T'CCnGG_A
Cuts at: 2429 2745 2429
Size: 316 5700
PmeI GTTT'AAAC
Cuts at: 3320 3320
Size: 6016
PmlI CAC'GTG
Cuts at: 3223 3223
Size: 6016
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4085 4117 5598 5630 4085
Size: 32 1481 32 4471
Fragments arranged by size:
4471 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2341 2857 2341
Size: 516 5500
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6016
PsiI TTA'TAA
Cuts at: 3586 3586
Size: 6016
PspOMI G'GGCC_C
Cuts at: 2068 2993 2068
Size: 925 5091
PstI C_TGCA'G
Cuts at: 830 3368 830
Size: 2538 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6016
PvuII CAG'CTG
Cuts at: 2019 2019
Size: 6016
SacI G_AGCT'C
Cuts at: 2556 2754 2556
Size: 198 5818
SacII CC_GC'GG
Cuts at: 2271 2271
Size: 6016
SalI G'TCGA_C
Cuts at: 1936 3358 1936
Size: 1422 4594
SapI GCTCTTCn'nnn_
Cuts at: 2573 2573
Size: 6016
SbfI CC_TGCA'GG
Cuts at: 3368 3368
Size: 6016
ScaI AGT'ACT
Cuts at: 2681 4264 5976 2681
Size: 1583 1712 2721
Fragments arranged by size:
2721 1712 1583
SexAI A'CCwGG_T
Cuts at: 3139 3139
Size: 6016
SfcI C'TryA_G
Cuts at: 826 1045 3364 4499 5180 5371 826
Size: 219 2319 1135 681 191 1471
Fragments arranged by size:
2319 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6016
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6016
SmaI CCC'GGG
Cuts at: 1872 2339 3343 1872
Size: 467 1004 4545
Fragments arranged by size:
4545 1004 467
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2795 3311 4120 5021 5283 820
Size: 197 926 852 516 809 901 262 1553
Fragments arranged by size:
1553 926 901 852 809 516 262 197
SnaBI TAC'GTA
Cuts at: 493 3807 493
Size: 3314 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6016
SphI G_CATG'C
Cuts at: 1132 3374 1132
Size: 2242 3774
SspI AAT'ATT
Cuts at: 5 52 3940 5
Size: 47 3888 2081
Fragments arranged by size:
3888 2081 47
StuI AGG'CCT
Cuts at: 1517 2810 1517
Size: 1293 4723
StyI C'CwwG_G
Cuts at: 513 1065 2004 2573 3455 513
Size: 552 939 569 882 3074
Fragments arranged by size:
3074 939 882 569 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2846 4043 4060 4213 4398 4843 325
Size: 1255 1266 1197 17 153 185 445 1498
Fragments arranged by size:
1498 1266 1255 1197 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2679 3106 4262
Size: 274 80 33 51 1443 702 427 1156
Cuts at: 4262 5974 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1156 702 427 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3212 3244 5976 6008 3212
Size: 32 2732 32 3220
Fragments arranged by size:
3220 2732 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6016
XbaI T'CTAG_A
Cuts at: 3352 3352
Size: 6016
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2401 2822 2401
Size: 421 5595
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6016
XmaI C'CCGG_G
Cuts at: 1870 2337 3341 1870
Size: 467 1004 4545
Fragments arranged by size:
4545 1004 467
XmnI GAAnn'nnTTC
Cuts at: 4145 4145
Size: 6016
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3824 276
Size: 53 83 186 936 2290 2468
Fragments arranged by size:
2468 2290 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NruI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NsiI PacI PspXI PsrI
RsrII SanDI SfiI SfoI SrfI SwaI