Restriction map for
pFN21AE5507
(Circular) (Six-base) MAPSORT of: pFN21AE5507.seq Check: 1980 from: 1 to: 5947
pFN21AE5507 5947 bp
With 182 enzymes: SgfI *
January 30, 2014 17:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3757 278
Size: 53 83 186 936 2221 2468
Fragments arranged by size:
2468 2221 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3186 3290 1937
Size: 1249 104 4594
Fragments arranged by size:
4594 1249 104
Acc65I G'GTAC_C
Cuts at: 3268 3268
Size: 5947
AclI AA'CG_TT
Cuts at: 1563 4074 4447 1563
Size: 2511 373 3063
Fragments arranged by size:
3063 2511 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2232 2829 4010 5394 5662 1632
Size: 600 597 1181 1384 268 1917
Fragments arranged by size:
1917 1384 1181 600 597 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5750
AflIII A'CryG_T
Cuts at: 1203 2776 4846 5823 1203
Size: 1573 2070 977 1327
Fragments arranged by size:
2070 1573 1327 977
AgeI A'CCGG_T
Cuts at: 4778 4778
Size: 5947
AhdI GACnn_n'nnGTC
Cuts at: 4676 4676
Size: 5947
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5947
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3055 1184
Size: 1871 4076
AlwNI CAG_nnn'CTG
Cuts at: 2156 2159 3690 5262 2156
Size: 3 1531 1572 2841
Fragments arranged by size:
2841 1572 1531 3
ApaLI G'TGCA_C
Cuts at: 3040 4004 5160 3040
Size: 964 1156 3827
Fragments arranged by size:
3827 1156 964
ApoI r'AATT_y
Cuts at: 1493 2066 2489 3018 3256 3482 3634 5743 1493
Size: 573 423 529 238 226 152 2109 1697
Fragments arranged by size:
2109 1697 573 529 423 238 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4501 160
Size: 872 3469 1606
Fragments arranged by size:
3469 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
AvaI C'yCGr_G
Cuts at: 1870 1943 3272 1870
Size: 73 1329 4545
Fragments arranged by size:
4545 1329 73
BamHI G'GATC_C
Cuts at: 3277 3644 3277
Size: 367 5580
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3268 4723 618
Size: 325 217 627 1481 1455 1842
Fragments arranged by size:
1842 1481 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3013 3266 1391
Size: 1622 253 4072
Fragments arranged by size:
4072 1622 253
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2629 2835 928
Size: 971 730 206 4040
Fragments arranged by size:
4040 971 730 206
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4138 4172 1903
Size: 34 50 34 2117 34 3678
Fragments arranged by size:
3678 2117 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3839 5049 3839
Size: 1210 4737
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5947
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4558 136
Size: 107 122 71 631 605 2886 1525
Fragments arranged by size:
2886 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5942 5942
Size: 5947
BlpI GC'TnA_GC
Cuts at: 3364 3364
Size: 5947
Bme1580I G_kGCm'C
Cuts at: 1790 3044 4008 5164 1790
Size: 1254 964 1156 2573
Fragments arranged by size:
2573 1254 1156 964
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5947
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3678 4636 442
Size: 416 53 83 399 24 2261 958 1753
Fragments arranged by size:
2261 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5947
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2229 2538 4607 1239
Size: 990 309 2069 2579
Fragments arranged by size:
2579 2069 990 309
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4072 4937 5235 4072
Size: 865 298 4784
Fragments arranged by size:
4784 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4610 882
Size: 617 3111 2219
Fragments arranged by size:
3111 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3119 3738 493
Size: 711 1915 619 2702
Fragments arranged by size:
2702 1915 711 619
BsaBI GATnn'nnATC
Cuts at: 5941 5941
Size: 5947
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3754 4136 4771 275
Size: 53 83 186 936 2221 382 635 1451
Fragments arranged by size:
2221 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4380 4778 5052 5199 5610 4380
Size: 398 274 147 411 4717
Fragments arranged by size:
4717 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5722
Fragments arranged by size:
5722 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3048 1186
Size: 1862 4085
BseYI C'CCAG_C
Cuts at: 5150 5646 5150
Size: 496 5451
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5374
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3397 4158 5186 1540
Size: 275 186 1396 761 1028 2301
Fragments arranged by size:
2301 1396 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2654 3013 3044 4008 4093 5164 2654
Size: 359 31 964 85 1071 3437
Fragments arranged by size:
3437 1071 964 359 85 31
BsmI GAATG_Cn'
Cuts at: 3458 3551 3458
Size: 93 5854
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2654 3013 3044 3266 4008 4093 5164
Size: 399 864 359 31 222 742 85 1071
Cuts at: 5164 1391
Size: 2174
Fragments arranged by size:
2174 1071 864 742 399 359 222 85
31
BspEI T'CCGG_A
Cuts at: 5610 5610
Size: 5947
BspHI T'CATG_A
Cuts at: 3836 5566 3836
Size: 1730 4217
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3302 844
Size: 2458 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3834 1394
Size: 429 2011 3507
Fragments arranged by size:
3507 2011 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2856 4442 4616 65
Size: 1183 1608 1586 174 1396
Fragments arranged by size:
1608 1586 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4591 4778 1700
Size: 110 2781 187 2869
Fragments arranged by size:
2869 2781 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5947
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5947
BssSI C'ACGA_G
Cuts at: 4007 5019 4007
Size: 1012 4935
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2858 794
Size: 2064 3883
BstBI TT'CG_AA
Cuts at: 4751 4751
Size: 5947
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5947
BstYI r'GATC_y
Cuts at: 1972 3277 3644 4029 4046 5498 5584 5596 5942
Size: 1305 367 385 17 1452 86 12 346
Cuts at: 5942 1972
Size: 1977
Fragments arranged by size:
1977 1452 1305 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5395
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5208
Fragments arranged by size:
5208 683 56
BtsI GCAGTG_nn'
Cuts at: 3463 4257 4277 3463
Size: 794 20 5133
Fragments arranged by size:
5133 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5947
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5912
DraI TTT'AAA
Cuts at: 2041 2223 3251 2041
Size: 182 1028 4737
Fragments arranged by size:
4737 1028 182
DraIII CAC_nnn'GTG
Cuts at: 2597 2597
Size: 5947
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4954 809
Size: 507 3638 1802
Fragments arranged by size:
3638 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2688 3394 4283
Size: 54 1425 50 275 18 858 706 889
Cuts at: 4283 5912 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 858 706 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3394 1537
Size: 275 1582 4090
Fragments arranged by size:
4090 1582 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3877 1353
Size: 2524 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4534 4908 5054 387
Size: 1064 138 2945 374 146 1280
Fragments arranged by size:
2945 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3011 3011
Size: 5947
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2229 2232 2538 2829 4010 4607 5394
Size: 393 597 3 306 291 1181 597 787
Cuts at: 5394 5662 1239
Size: 268 1524
Fragments arranged by size:
1524 1181 787 597 597 393 306 291
268 3
EcoRI G'AATT_C
Cuts at: 3256 3634 3256
Size: 378 5569
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2315 2646 2315
Size: 331 5616
FspI TGC'GCA
Cuts at: 4453 4453
Size: 5947
HaeII r_GCGC'y
Cuts at: 1678 5094 1678
Size: 3416 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2227 2259 4588 4620 4662
Size: 32 135 32 655 32 2329 32 42
Cuts at: 4662 4694 1373
Size: 32 2626
Fragments arranged by size:
2626 2329 655 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2475 3291 3537 669
Size: 988 281 537 816 246 3079
Fragments arranged by size:
3079 988 816 537 281 246
HindIII A'AGCT_T
Cuts at: 748 3210 748
Size: 2462 3485
HpaI GTT'AAC
Cuts at: 3537 3537
Size: 5947
KpnI G_GTAC'C
Cuts at: 3272 3272
Size: 5947
MfeI C'AATT_G
Cuts at: 2337 3546 2337
Size: 1209 4738
MluI A'CGCG_T
Cuts at: 5823 5823
Size: 5947
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3184 5061 5245 1305
Size: 1879 1877 184 2007
Fragments arranged by size:
2007 1879 1877 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2690 10
Size: 54 1425 343 858 3267
Fragments arranged by size:
3267 1425 858 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3905 4264 4423 518
Size: 648 2739 359 159 2042
Fragments arranged by size:
2739 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2156 2404 3363 4040 5188 5433 1606
Size: 550 248 959 677 1148 245 2120
Fragments arranged by size:
2120 1148 959 677 550 248 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5395
NdeI CA'TA_TG
Cuts at: 387 2445 387
Size: 2058 3889
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5947
NotI GC'GGCC_GC
Cuts at: 3394 3394
Size: 5947
NspI r_CATG'y
Cuts at: 1132 1229 2605 2889 3305 4850 1132
Size: 97 1376 284 416 1545 2229
Fragments arranged by size:
2229 1545 1376 416 284 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5947
PciI A'CATG_T
Cuts at: 4846 4846
Size: 5947
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5947
PfoI T'CCnGG_A
Cuts at: 2092 2092
Size: 5947
PmeI GTTT'AAAC
Cuts at: 3251 3251
Size: 5947
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4016 4048 5529 5561 4016
Size: 32 1481 32 4402
Fragments arranged by size:
4402 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5947
PsiI TTA'TAA
Cuts at: 3517 3517
Size: 5947
PstI C_TGCA'G
Cuts at: 830 2029 3299 830
Size: 1199 1270 3478
Fragments arranged by size:
3478 1270 1199
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5947
PvuII CAG'CTG
Cuts at: 2156 2404 2156
Size: 248 5699
SacI G_AGCT'C
Cuts at: 3013 3013
Size: 5947
SalI G'TCGA_C
Cuts at: 1936 3289 1936
Size: 1353 4594
SbfI CC_TGCA'GG
Cuts at: 3299 3299
Size: 5947
ScaI AGT'ACT
Cuts at: 2964 4195 5907 2964
Size: 1231 1712 3004
Fragments arranged by size:
3004 1712 1231
SexAI A'CCwGG_T
Cuts at: 2593 2593
Size: 5947
SfcI C'TryA_G
Cuts at: 826 1045 2025 3295 4430 5111 5302 826
Size: 219 980 1270 1135 681 191 1471
Fragments arranged by size:
1471 1270 1135 980 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5947
SmaI CCC'GGG
Cuts at: 1872 3274 1872
Size: 1402 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4051 4952 5214 820
Size: 197 926 2108 901 262 1553
Fragments arranged by size:
2108 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3738 493
Size: 3245 2702
SpeI A'CTAG_T
Cuts at: 152 2135 152
Size: 1983 3964
SphI G_CATG'C
Cuts at: 1132 3305 1132
Size: 2173 3774
SspI AAT'ATT
Cuts at: 5 52 2275 3871 5
Size: 47 2223 1596 2081
Fragments arranged by size:
2223 2081 1596 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5947
StyI C'CwwG_G
Cuts at: 513 1065 3386 513
Size: 552 2321 3074
Fragments arranged by size:
3074 2321 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3974 3991 4144 4329 4774 325
Size: 1255 2394 17 153 185 445 1498
Fragments arranged by size:
2394 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2287 2869 2962
Size: 274 80 33 51 1443 310 582 93
Cuts at: 2962 4193 5905 96
Size: 1231 1712 138
Fragments arranged by size:
1712 1443 1231 582 310 274 138 93
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5907 5939 5907
Size: 32 5915
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5947
XbaI T'CTAG_A
Cuts at: 3283 3283
Size: 5947
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2443 2683 2443
Size: 240 5707
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5947
XmaI C'CCGG_G
Cuts at: 1870 3272 1870
Size: 1402 4545
XmnI GAAnn'nnTTC
Cuts at: 2274 2626 4076 2274
Size: 352 1450 4145
Fragments arranged by size:
4145 1450 352
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3755 276
Size: 53 83 186 936 2221 2468
Fragments arranged by size:
2468 2221 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI
SrfI SwaI