Restriction map for
pFN21AE5505
(Circular) (Six-base) MAPSORT of: pFN21AE5505.seq Check: 5195 from: 1 to: 5965
pFN21AE5505 5965 bp
With 182 enzymes: SgfI *
January 30, 2014 17:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3775 278
Size: 53 83 186 936 2239 2468
Fragments arranged by size:
2468 2239 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2715 3308 1937
Size: 778 593 4594
Fragments arranged by size:
4594 778 593
Acc65I G'GTAC_C
Cuts at: 3286 3286
Size: 5965
AclI AA'CG_TT
Cuts at: 1563 4092 4465 1563
Size: 2529 373 3063
Fragments arranged by size:
3063 2529 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2892 4028 5412 5680 1632
Size: 1260 1136 1384 268 1917
Fragments arranged by size:
1917 1384 1260 1136 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5768
AflIII A'CryG_T
Cuts at: 1203 4864 5841 1203
Size: 3661 977 1327
Fragments arranged by size:
3661 1327 977
AgeI A'CCGG_T
Cuts at: 4796 4796
Size: 5965
AhdI GACnn_n'nnGTC
Cuts at: 4694 4694
Size: 5965
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5965
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5965
AlwNI CAG_nnn'CTG
Cuts at: 2376 2960 3708 5280 2376
Size: 584 748 1572 3061
Fragments arranged by size:
3061 1572 748 584
ApaI G_GGCC'C
Cuts at: 2354 2354
Size: 5965
ApaLI G'TGCA_C
Cuts at: 4022 5178 4022
Size: 1156 4809
ApoI r'AATT_y
Cuts at: 1493 3274 3500 3652 5761 1493
Size: 1781 226 152 2109 1697
Fragments arranged by size:
2109 1781 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4519 160
Size: 872 3487 1606
Fragments arranged by size:
3487 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5965
AvaI C'yCGr_G
Cuts at: 1870 1943 3290 1870
Size: 73 1347 4545
Fragments arranged by size:
4545 1347 73
BamHI G'GATC_C
Cuts at: 3295 3662 3295
Size: 367 5598
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3286 4741 618
Size: 325 217 627 1499 1455 1842
Fragments arranged by size:
1842 1499 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2354 3284 1391
Size: 963 930 4072
Fragments arranged by size:
4072 963 930
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2288 2461 2601 3151 928
Size: 971 389 173 140 550 3742
Fragments arranged by size:
3742 971 550 389 173 140
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4156 4190 1903
Size: 34 2219 34 3678
Fragments arranged by size:
3678 2219 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3857 5067 3857
Size: 1210 4755
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5965
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4576 136
Size: 107 122 71 631 605 2904 1525
Fragments arranged by size:
2904 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2645 5960 2645
Size: 3315 2650
BlpI GC'TnA_GC
Cuts at: 3382 3382
Size: 5965
Bme1580I G_kGCm'C
Cuts at: 1790 2354 4026 5182 1790
Size: 564 1672 1156 2573
Fragments arranged by size:
2573 1672 1156 564
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5965
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2414 3696 4654
Size: 416 53 83 399 24 997 1282 958
Cuts at: 4654 442
Size: 1753
Fragments arranged by size:
1753 1282 997 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5965
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4625 1239
Size: 3386 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4090 4955 5253 4090
Size: 865 298 4802
Fragments arranged by size:
4802 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4628 882
Size: 617 3129 2219
Fragments arranged by size:
3129 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3756 493
Size: 711 2552 2702
Fragments arranged by size:
2702 2552 711
BsaBI GATnn'nnATC
Cuts at: 3014 5959 3014
Size: 2945 3020
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3772 4154 4789 275
Size: 53 83 186 936 2239 382 635 1451
Fragments arranged by size:
2239 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4398 4796 5070 5217 5628 4398
Size: 398 274 147 411 4735
Fragments arranged by size:
4735 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5740
Fragments arranged by size:
5740 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5965
BseYI C'CCAG_C
Cuts at: 2226 5168 5664 2226
Size: 2942 496 2527
Fragments arranged by size:
2942 2527 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5392
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3415 4176 5204 1540
Size: 275 186 1414 761 1028 2301
Fragments arranged by size:
2301 1414 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4026 4111 5182 4026
Size: 85 1071 4809
Fragments arranged by size:
4809 1071 85
BsmI GAATG_Cn'
Cuts at: 3476 3569 3476
Size: 93 5872
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2354 3284 4026 4111 5182 1391
Size: 399 564 930 742 85 1071 2174
Fragments arranged by size:
2174 1071 930 742 564 399 85
BspEI T'CCGG_A
Cuts at: 5628 5628
Size: 5965
BspHI T'CATG_A
Cuts at: 2178 3854 5584 2178
Size: 1676 1730 2559
Fragments arranged by size:
2559 1730 1676
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3320 844
Size: 2476 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3852 1394
Size: 429 2029 3507
Fragments arranged by size:
3507 2029 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2382 4460 4634 65
Size: 1183 1134 2078 174 1396
Fragments arranged by size:
2078 1396 1183 1134 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4609 4796 1700
Size: 110 2799 187 2869
Fragments arranged by size:
2869 2799 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5965
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5965
BssSI C'ACGA_G
Cuts at: 4025 5037 4025
Size: 1012 4953
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2376 794
Size: 1582 4383
BstBI TT'CG_AA
Cuts at: 4769 4769
Size: 5965
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5965
BstYI r'GATC_y
Cuts at: 1972 2645 3295 3662 4047 4064 5516 5602 5614
Size: 673 650 367 385 17 1452 86 12
Cuts at: 5614 5960 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 673 650 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2716 2716
Size: 5965
BtgI C'CryG_G
Cuts at: 513 1065 2004 2190 2199 2549 513
Size: 552 939 186 9 350 3929
Fragments arranged by size:
3929 939 552 350 186 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5226
Fragments arranged by size:
5226 683 56
BtsI GCAGTG_nn'
Cuts at: 3481 4275 4295 3481
Size: 794 20 5151
Fragments arranged by size:
5151 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5965
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5930
DraI TTT'AAA
Cuts at: 3269 3269
Size: 5965
DraIII CAC_nnn'GTG
Cuts at: 2997 2997
Size: 5965
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4972 809
Size: 507 3656 1802
Fragments arranged by size:
3656 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3412 4301
Size: 54 1425 50 275 18 177 1405 889
Cuts at: 4301 5930 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1405 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3412 1537
Size: 275 1600 4090
Fragments arranged by size:
4090 1600 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2628 2911 3203 3895 1353
Size: 1275 283 292 692 3423
Fragments arranged by size:
3423 1275 692 292 283
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4552 4926 5072 387
Size: 1064 138 2963 374 146 1280
Fragments arranged by size:
2963 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2892 4028 4625 5412 5680 1239
Size: 393 1260 1136 597 787 268 1524
Fragments arranged by size:
1524 1260 1136 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2350 2351 3133 2350
Size: 1 782 5182
Fragments arranged by size:
5182 782 1
EcoRI G'AATT_C
Cuts at: 3274 3652 3274
Size: 378 5587
FspI TGC'GCA
Cuts at: 4471 4471
Size: 5965
HaeII r_GCGC'y
Cuts at: 1678 5112 1678
Size: 3434 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4606 4638 4680 4712 1373
Size: 32 135 32 3034 32 42 32 2626
Fragments arranged by size:
3034 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2206 2260 2752 3181 3309 3555
Size: 988 281 268 54 492 429 128 246
Cuts at: 3555 669
Size: 3079
Fragments arranged by size:
3079 988 492 429 281 268 246 128
54
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5965
HpaI GTT'AAC
Cuts at: 2752 3181 3555 2752
Size: 429 374 5162
Fragments arranged by size:
5162 429 374
KpnI G_GTAC'C
Cuts at: 3290 3290
Size: 5965
MfeI C'AATT_G
Cuts at: 3564 3564
Size: 5965
MluI A'CGCG_T
Cuts at: 5841 5841
Size: 5965
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5079 5263 1305
Size: 3774 184 2007
Fragments arranged by size:
3774 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 3966
Fragments arranged by size:
3966 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2189 2810 2867 3923 4282 4441 518
Size: 648 1023 621 57 1056 359 159 2042
Fragments arranged by size:
2042 1056 1023 648 621 359 159 57
MspA1I CmG'CkG
Cuts at: 1606 3114 3381 4058 5206 5451 1606
Size: 1508 267 677 1148 245 2120
Fragments arranged by size:
2120 1508 1148 677 267 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2190 2199 2549 513
Size: 552 939 186 9 350 3929
Fragments arranged by size:
3929 939 552 350 186 9
NdeI CA'TA_TG
Cuts at: 387 2666 387
Size: 2279 3686
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5965
NotI GC'GGCC_GC
Cuts at: 3412 3412
Size: 5965
NspI r_CATG'y
Cuts at: 1132 1229 3323 4868 1132
Size: 97 2094 1545 2229
Fragments arranged by size:
2229 2094 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5965
PciI A'CATG_T
Cuts at: 4864 4864
Size: 5965
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5965
PmeI GTTT'AAAC
Cuts at: 3269 3269
Size: 5965
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4034 4066 5547 5579 4034
Size: 32 1481 32 4420
Fragments arranged by size:
4420 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3133 3133
Size: 5965
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5965
PsiI TTA'TAA
Cuts at: 3535 3535
Size: 5965
PspOMI G'GGCC_C
Cuts at: 2350 2350
Size: 5965
PstI C_TGCA'G
Cuts at: 830 2381 3317 830
Size: 1551 936 3478
Fragments arranged by size:
3478 1551 936
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5965
SalI G'TCGA_C
Cuts at: 1936 3307 1936
Size: 1371 4594
SbfI CC_TGCA'GG
Cuts at: 3317 3317
Size: 5965
ScaI AGT'ACT
Cuts at: 4213 5925 4213
Size: 1712 4253
SfcI C'TryA_G
Cuts at: 826 1045 2377 3313 4448 5129 5320 826
Size: 219 1332 936 1135 681 191 1471
Fragments arranged by size:
1471 1332 1135 936 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5965
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5965
SmaI CCC'GGG
Cuts at: 1872 3292 1872
Size: 1420 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4069 4970 5232 820
Size: 197 926 2126 901 262 1553
Fragments arranged by size:
2126 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3756 493
Size: 3263 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5965
SphI G_CATG'C
Cuts at: 1132 3323 1132
Size: 2191 3774
SspI AAT'ATT
Cuts at: 5 52 3889 5
Size: 47 3837 2081
Fragments arranged by size:
3837 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5965
StyI C'CwwG_G
Cuts at: 513 1065 2004 2190 2199 2452 2549 3038 3404
Size: 552 939 186 9 253 97 489 366
Cuts at: 3404 513
Size: 3074
Fragments arranged by size:
3074 939 552 489 366 253 186 97
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2445 3135 3992 4009 4162 4347 4792
Size: 1255 865 690 857 17 153 185 445
Cuts at: 4792 325
Size: 1498
Fragments arranged by size:
1498 1255 865 857 690 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4211 5923 96
Size: 274 80 33 51 1443 2234 1712 138
Fragments arranged by size:
2234 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5925 5957 5925
Size: 32 5933
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5965
XbaI T'CTAG_A
Cuts at: 3301 3301
Size: 5965
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2197 3055 2197
Size: 858 5107
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5965
XmaI C'CCGG_G
Cuts at: 1870 3290 1870
Size: 1420 4545
XmnI GAAnn'nnTTC
Cuts at: 3158 4094 3158
Size: 936 5029
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3773 276
Size: 53 83 186 936 2239 2468
Fragments arranged by size:
2468 2239 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI