Restriction map for
pFN21AE5501
(Circular) (Six-base) MAPSORT of: pFN21AE5501.seq Check: 4373 from: 1 to: 5962
pFN21AE5501 5962 bp
With 182 enzymes: SgfI *
January 30, 2014 17:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2154 2154
Size: 5962
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2199 3772 278
Size: 53 83 186 936 663 1573 2468
Fragments arranged by size:
2468 1573 936 663 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2874 3305 1937
Size: 937 431 4594
Fragments arranged by size:
4594 937 431
Acc65I G'GTAC_C
Cuts at: 3283 3283
Size: 5962
AclI AA'CG_TT
Cuts at: 1563 4089 4462 1563
Size: 2526 373 3063
Fragments arranged by size:
3063 2526 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2504 4025 5409 5677 1632
Size: 872 1521 1384 268 1917
Fragments arranged by size:
1917 1521 1384 872 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5765
AflIII A'CryG_T
Cuts at: 1203 4861 5838 1203
Size: 3658 977 1327
Fragments arranged by size:
3658 1327 977
AgeI A'CCGG_T
Cuts at: 4793 4793
Size: 5962
AhdI GACnn_n'nnGTC
Cuts at: 2284 4691 2284
Size: 2407 3555
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5962
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5962
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2300 2332 2300
Size: 32 5930
AlwNI CAG_nnn'CTG
Cuts at: 2290 2772 2975 3705 5277 2290
Size: 482 203 730 1572 2975
Fragments arranged by size:
2975 1572 730 482 203
ApaI G_GGCC'C
Cuts at: 2171 2171
Size: 5962
ApaLI G'TGCA_C
Cuts at: 4019 5175 4019
Size: 1156 4806
ApoI r'AATT_y
Cuts at: 1493 3271 3497 3649 5758 1493
Size: 1778 226 152 2109 1697
Fragments arranged by size:
2109 1778 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4516 160
Size: 872 3484 1606
Fragments arranged by size:
3484 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
AvaI C'yCGr_G
Cuts at: 1870 1943 2331 2544 3287 1870
Size: 73 388 213 743 4545
Fragments arranged by size:
4545 743 388 213 73
BamHI G'GATC_C
Cuts at: 3292 3659 3292
Size: 367 5595
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3283 4738 618
Size: 325 217 627 1496 1455 1842
Fragments arranged by size:
1842 1496 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2171 3147 3281 1391
Size: 780 976 134 4072
Fragments arranged by size:
4072 976 780 134
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4991
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4153 4187 1903
Size: 34 50 34 2132 34 3678
Fragments arranged by size:
3678 2132 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2955 3854 5064 2955
Size: 899 1210 3853
Fragments arranged by size:
3853 1210 899
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5962
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2102 4573 136
Size: 107 122 71 631 605 430 2471 1525
Fragments arranged by size:
2471 1525 631 605 430 122 107 71
BglII A'GATC_T
Cuts at: 2232 2385 5957 2232
Size: 153 3572 2237
Fragments arranged by size:
3572 2237 153
BlpI GC'TnA_GC
Cuts at: 3379 3379
Size: 5962
Bme1580I G_kGCm'C
Cuts at: 1790 2171 2208 4023 5179 1790
Size: 381 37 1815 1156 2573
Fragments arranged by size:
2573 1815 1156 381 37
BmgBI CAC'GTC
Cuts at: 1318 2281 3213 1318
Size: 963 932 4067
Fragments arranged by size:
4067 963 932
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2782 3165 3693
Size: 416 53 83 399 24 1365 383 528
Cuts at: 3693 4651 442
Size: 958 1753
Fragments arranged by size:
1753 1365 958 528 416 399 383 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5962
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2150 2159 2261 2399 2675 4622 1239
Size: 911 9 102 138 276 1947 2579
Fragments arranged by size:
2579 1947 911 276 138 102 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2580 4087 4952 5250 2580
Size: 1507 865 298 3292
Fragments arranged by size:
3292 1507 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2258 4625 882
Size: 617 759 2367 2219
Fragments arranged by size:
2367 2219 759 617
BsaAI yAC'GTr
Cuts at: 493 1204 2479 3753 493
Size: 711 1275 1274 2702
Fragments arranged by size:
2702 1275 1274 711
BsaBI GATnn'nnATC
Cuts at: 5956 5956
Size: 5962
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2196 2467 3769 4151
Size: 53 83 186 936 663 271 1302 382
Cuts at: 4151 4786 275
Size: 635 1451
Fragments arranged by size:
1451 1302 936 663 635 382 271 186
83 53
BsaWI w'CCGG_w
Cuts at: 2325 2795 4395 4793 5067 5214 5625 2325
Size: 470 1600 398 274 147 411 2662
Fragments arranged by size:
2662 1600 470 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2168 2198 2300 2330 2849
Size: 30 165 30 765 30 102 30 519
Cuts at: 2849 2879 2912 2942 1178
Size: 30 33 30 4198
Fragments arranged by size:
4198 765 519 165 102 33 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2315 2396 2927 1186
Size: 1129 81 531 4221
Fragments arranged by size:
4221 1129 531 81
BseYI C'CCAG_C
Cuts at: 2213 2931 5165 5661 2213
Size: 718 2234 496 2514
Fragments arranged by size:
2514 2234 718 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2303 1163
Size: 573 567 4822
Fragments arranged by size:
4822 573 567
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3412 4173 5201 1540
Size: 275 186 1411 761 1028 2301
Fragments arranged by size:
2301 1411 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2082 2145 3147 3243 4023 4108 5179 2082
Size: 63 1002 96 780 85 1071 2865
Fragments arranged by size:
2865 1071 1002 780 96 85 63
BsmI GAATG_Cn'
Cuts at: 3473 3566 3473
Size: 93 5869
BsmBI CGTCTCn'nnnn_
Cuts at: 3203 3203
Size: 5962
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2082 2145 2171 2208 3147 3243 3281
Size: 399 292 63 26 37 939 96 38
Cuts at: 3281 4023 4108 5179 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 939 742 399 292 96 85
63 38 37 26
BspEI T'CCGG_A
Cuts at: 2795 5625 2795
Size: 2830 3132
BspHI T'CATG_A
Cuts at: 3851 5581 3851
Size: 1730 4232
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2154 2412 3317 844
Size: 1310 258 905 3489
Fragments arranged by size:
3489 1310 905 258
BsrBI CCG'CTC
Cuts at: 1394 1823 3849 1394
Size: 429 2026 3507
Fragments arranged by size:
3507 2026 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4457 4631 65
Size: 1183 3209 174 1396
Fragments arranged by size:
3209 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4606 4793 1700
Size: 110 2796 187 2869
Fragments arranged by size:
2869 2796 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5962
BssHII G'CGCG_C
Cuts at: 1925 2336 1925
Size: 411 5551
BssSI C'ACGA_G
Cuts at: 4022 5034 4022
Size: 1012 4950
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2077 2290 794
Size: 1283 213 4466
Fragments arranged by size:
4466 1283 213
BstBI TT'CG_AA
Cuts at: 4766 4766
Size: 5962
BstEII G'GTnAC_C
Cuts at: 1184 2554 1184
Size: 1370 4592
BstXI CCAn_nnnn'nTGG
Cuts at: 2520 2567 2520
Size: 47 5915
BstYI r'GATC_y
Cuts at: 1972 2232 2385 2439 3292 3659 4044 4061 5513
Size: 260 153 54 853 367 385 17 1452
Cuts at: 5513 5599 5611 5957 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 853 385 367 346 260 153
86 54 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2248 2830 2890 513
Size: 552 1183 582 60 3585
Fragments arranged by size:
3585 1183 582 552 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5223
Fragments arranged by size:
5223 683 56
BtsI GCAGTG_nn'
Cuts at: 3478 4272 4292 3478
Size: 794 20 5148
Fragments arranged by size:
5148 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5962
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5927
DraI TTT'AAA
Cuts at: 3266 3266
Size: 5962
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4969 809
Size: 507 3653 1802
Fragments arranged by size:
3653 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3409 4298 5927
Size: 54 1425 50 275 18 1579 889 1629
Cuts at: 5927 8
Size: 43
Fragments arranged by size:
1629 1579 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3409 1537
Size: 275 1597 4090
Fragments arranged by size:
4090 1597 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3892 1353
Size: 2539 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2429 4549 4923 5069 387
Size: 1064 138 840 2120 374 146 1280
Fragments arranged by size:
2120 1280 1064 840 374 146 138
EcoICRI GAG'CTC
Cuts at: 3145 3145
Size: 5962
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2150 2159 2261 2399 2504 2675 4025
Size: 393 518 9 102 138 105 171 1350
Cuts at: 4025 4622 5409 5677 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1350 787 597 518 393 268 171
138 105 102 9
EcoO109I rG'GnC_Cy
Cuts at: 2134 2167 2168 2134
Size: 33 1 5928
Fragments arranged by size:
5928 33 1
EcoRI G'AATT_C
Cuts at: 3271 3649 3271
Size: 378 5584
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2077 3148 2077
Size: 1071 4891
FspI TGC'GCA
Cuts at: 4468 4468
Size: 5962
HaeII r_GCGC'y
Cuts at: 1678 3163 3224 5109 1678
Size: 1485 61 1885 2531
Fragments arranged by size:
2531 1885 1485 61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2270 2302 2849 2881 4603
Size: 32 135 32 698 32 547 32 1722
Cuts at: 4603 4635 4677 4709 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1722 698 547 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3306 3552 669
Size: 988 281 1368 246 3079
Fragments arranged by size:
3079 1368 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5962
HpaI GTT'AAC
Cuts at: 3552 3552
Size: 5962
KpnI G_GTAC'C
Cuts at: 3287 3287
Size: 5962
MfeI C'AATT_G
Cuts at: 3561 3561
Size: 5962
MluI A'CGCG_T
Cuts at: 5838 5838
Size: 5962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5076 5260 1305
Size: 3771 184 2007
Fragments arranged by size:
3771 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4140
Fragments arranged by size:
4140 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2477 3920 4279 4438 518
Size: 648 1311 1443 359 159 2042
Fragments arranged by size:
2042 1443 1311 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2152 2832 3378 4055 5203 5448 1606
Size: 546 680 546 677 1148 245 2120
Fragments arranged by size:
2120 1148 680 677 546 546 245
NcoI C'CATG_G
Cuts at: 513 1065 2890 513
Size: 552 1825 3585
Fragments arranged by size:
3585 1825 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5962
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5962
NotI GC'GGCC_GC
Cuts at: 3409 3409
Size: 5962
NspI r_CATG'y
Cuts at: 1132 1229 2710 3320 4865 1132
Size: 97 1481 610 1545 2229
Fragments arranged by size:
2229 1545 1481 610 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5962
PciI A'CATG_T
Cuts at: 4861 4861
Size: 5962
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2377 1108
Size: 1269 4693
PfoI T'CCnGG_A
Cuts at: 2397 2652 3147 2397
Size: 255 495 5212
Fragments arranged by size:
5212 495 255
PmeI GTTT'AAAC
Cuts at: 3266 3266
Size: 5962
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2300 2332 4031 4063 5544 5576 2300
Size: 32 1699 32 1481 32 2686
Fragments arranged by size:
2686 1699 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2134 2134
Size: 5962
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5962
PsiI TTA'TAA
Cuts at: 3532 3532
Size: 5962
PspOMI G'GGCC_C
Cuts at: 2167 2167
Size: 5962
PstI C_TGCA'G
Cuts at: 830 2151 3314 830
Size: 1321 1163 3478
Fragments arranged by size:
3478 1321 1163
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5962
PvuII CAG'CTG
Cuts at: 2152 2152
Size: 5962
SacI G_AGCT'C
Cuts at: 3147 3147
Size: 5962
SacII CC_GC'GG
Cuts at: 2833 2833
Size: 5962
SalI G'TCGA_C
Cuts at: 1936 3304 1936
Size: 1368 4594
SbfI CC_TGCA'GG
Cuts at: 3314 3314
Size: 5962
ScaI AGT'ACT
Cuts at: 3128 4210 5922 3128
Size: 1082 1712 3168
Fragments arranged by size:
3168 1712 1082
SfcI C'TryA_G
Cuts at: 826 1045 2091 2147 3109 3310 4445 5126 5317
Size: 219 1046 56 962 201 1135 681 191
Cuts at: 5317 826
Size: 1471
Fragments arranged by size:
1471 1135 1046 962 681 219 201 191
56
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5962
SmaI CCC'GGG
Cuts at: 1872 2333 2546 3289 1872
Size: 461 213 743 4545
Fragments arranged by size:
4545 743 461 213
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2595 4066 4967 5229 820
Size: 197 926 652 1471 901 262 1553
Fragments arranged by size:
1553 1471 926 901 652 262 197
SnaBI TAC'GTA
Cuts at: 493 3753 493
Size: 3260 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5962
SphI G_CATG'C
Cuts at: 1132 3320 1132
Size: 2188 3774
SspI AAT'ATT
Cuts at: 5 52 3886 5
Size: 47 3834 2081
Fragments arranged by size:
3834 2081 47
StuI AGG'CCT
Cuts at: 1517 2906 1517
Size: 1389 4573
StyI C'CwwG_G
Cuts at: 513 1065 2640 2890 3003 3204 3401 513
Size: 552 1575 250 113 201 197 3074
Fragments arranged by size:
3074 1575 552 250 201 197 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2573 2712 3989 4006 4159 4344 4789
Size: 1255 993 139 1277 17 153 185 445
Cuts at: 4789 325
Size: 1498
Fragments arranged by size:
1498 1277 1255 993 445 185 153 139
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3126 4208 5920
Size: 274 80 33 51 1443 1149 1082 1712
Cuts at: 5920 96
Size: 138
Fragments arranged by size:
1712 1443 1149 1082 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2168 2200 2912 2944 5922 5954 2168
Size: 32 712 32 2978 32 2176
Fragments arranged by size:
2978 2176 712 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2722 1316
Size: 1406 4556
XbaI T'CTAG_A
Cuts at: 3298 3298
Size: 5962
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2851 2851
Size: 5962
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5962
XmaI C'CCGG_G
Cuts at: 1870 2331 2544 3287 1870
Size: 461 213 743 4545
Fragments arranged by size:
4545 743 461 213
XmnI GAAnn'nnTTC
Cuts at: 4091 4091
Size: 5962
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2197 3770 276
Size: 53 83 186 936 663 1573 2468
Fragments arranged by size:
2468 1573 936 663 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SanDI SapI SexAI SfiI
SfoI SrfI SwaI