Restriction map for
pFN21AE5495
(Circular) (Six-base) MAPSORT of: pFN21AE5495.seq Check: 1429 from: 1 to: 5749
pFN21AE5495 5749 bp
With 182 enzymes: SgfI *
December 8, 2014 13:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2828 2828
Size: 5749
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3559 278
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3092 1937
Size: 1155 4594
Acc65I G'GTAC_C
Cuts at: 3070 3070
Size: 5749
AclI AA'CG_TT
Cuts at: 1563 3876 4249 1563
Size: 2313 373 3063
Fragments arranged by size:
3063 2313 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2053 2137 3023 3812 5196 5464 1632
Size: 421 84 886 789 1384 268 1917
Fragments arranged by size:
1917 1384 886 789 421 268 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5552
AflIII A'CryG_T
Cuts at: 1203 4648 5625 1203
Size: 3445 977 1327
Fragments arranged by size:
3445 1327 977
AgeI A'CCGG_T
Cuts at: 4580 4580
Size: 5749
AhdI GACnn_n'nnGTC
Cuts at: 4478 4478
Size: 5749
AleI CACnn'nnGTG
Cuts at: 1166 2948 1166
Size: 1782 3967
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5749
AlwNI CAG_nnn'CTG
Cuts at: 2384 3492 5064 2384
Size: 1108 1572 3069
Fragments arranged by size:
3069 1572 1108
ApaLI G'TGCA_C
Cuts at: 3806 4962 3806
Size: 1156 4593
ApoI r'AATT_y
Cuts at: 1493 2432 3058 3284 3436 5545 1493
Size: 939 626 226 152 2109 1697
Fragments arranged by size:
2109 1697 939 626 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4303 160
Size: 872 3271 1606
Fragments arranged by size:
3271 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
AvaI C'yCGr_G
Cuts at: 1870 1943 2613 3074 1870
Size: 73 670 461 4545
Fragments arranged by size:
4545 670 461 73
BamHI G'GATC_C
Cuts at: 2609 3079 3446 2609
Size: 470 367 4912
Fragments arranged by size:
4912 470 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3070 4525 618
Size: 325 217 627 1283 1455 1842
Fragments arranged by size:
1842 1455 1283 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2558 3001 3068 1391
Size: 1167 443 67 4072
Fragments arranged by size:
4072 1167 443 67
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2920 928
Size: 971 1021 3757
Fragments arranged by size:
3757 1021 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3940 3974 1903
Size: 34 2003 34 3678
Fragments arranged by size:
3678 2003 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3641 4851 3641
Size: 1210 4539
BclI T'GATC_A
Cuts at: 1548 2736 1548
Size: 1188 4561
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2453 4360 136
Size: 107 122 71 631 605 781 1907 1525
Fragments arranged by size:
1907 1525 781 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2373 5744 2373
Size: 3371 2378
BlpI GC'TnA_GC
Cuts at: 3166 3166
Size: 5749
Bme1580I G_kGCm'C
Cuts at: 1790 2337 3810 4966 1790
Size: 547 1473 1156 2573
Fragments arranged by size:
2573 1473 1156 547
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5749
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3480 4438 442
Size: 416 53 83 399 24 2063 958 1753
Fragments arranged by size:
2063 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5749
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2931 4409 1239
Size: 1692 1478 2579
Fragments arranged by size:
2579 1692 1478
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3874 4739 5037 3874
Size: 865 298 4586
Fragments arranged by size:
4586 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4412 882
Size: 617 2913 2219
Fragments arranged by size:
2913 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2413 2470 3540 493
Size: 711 1209 57 1070 2702
Fragments arranged by size:
2702 1209 1070 711 57
BsaBI GATnn'nnATC
Cuts at: 5743 5743
Size: 5749
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2256 3556 3938 4573
Size: 53 83 186 936 723 1300 382 635
Cuts at: 4573 275
Size: 1451
Fragments arranged by size:
1451 1300 936 723 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4182 4580 4854 5001 5412 4182
Size: 398 274 147 411 4519
Fragments arranged by size:
4519 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2563 2593 2748 2778 1178
Size: 30 165 30 1160 30 155 30 4149
Fragments arranged by size:
4149 1160 165 155 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2711 2756 2815 1186
Size: 1525 45 59 4120
Fragments arranged by size:
4120 1525 59 45
BseYI C'CCAG_C
Cuts at: 2885 4952 5448 2885
Size: 2067 496 3186
Fragments arranged by size:
3186 2067 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5176
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3199 3960 4988 1540
Size: 275 186 1198 761 1028 2301
Fragments arranged by size:
2301 1198 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3810 3895 4966 3810
Size: 85 1071 4593
Fragments arranged by size:
4593 1071 85
BsmI GAATG_Cn'
Cuts at: 3260 3353 3260
Size: 93 5656
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2337 2558 3001 3068 3810 3895 4966
Size: 399 547 221 443 67 742 85 1071
Cuts at: 4966 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 547 443 399 221 85
67
BspEI T'CCGG_A
Cuts at: 5412 5412
Size: 5749
BspHI T'CATG_A
Cuts at: 2505 3638 5368 2505
Size: 1133 1730 2886
Fragments arranged by size:
2886 1730 1133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2414 2828 3034 3104 844
Size: 1570 414 206 70 3489
Fragments arranged by size:
3489 1570 414 206 70
BsrBI CCG'CTC
Cuts at: 1394 1823 3636 1394
Size: 429 1813 3507
Fragments arranged by size:
3507 1813 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4244 4418 65
Size: 1183 2996 174 1396
Fragments arranged by size:
2996 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4393 4580 1700
Size: 110 2583 187 2869
Fragments arranged by size:
2869 2583 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5749
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5749
BssSI C'ACGA_G
Cuts at: 3809 4821 3809
Size: 1012 4737
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5749
BstBI TT'CG_AA
Cuts at: 4553 4553
Size: 5749
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5749
BstYI r'GATC_y
Cuts at: 1972 2373 2609 3079 3446 3831 3848 5300 5386
Size: 401 236 470 367 385 17 1452 86
Cuts at: 5386 5398 5744 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 470 401 385 367 346 236
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2451 2605 2776 513
Size: 552 939 447 154 171 3486
Fragments arranged by size:
3486 939 552 447 171 154
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5010
Fragments arranged by size:
5010 683 56
BtsI GCAGTG_nn'
Cuts at: 3265 4059 4079 3265
Size: 794 20 4935
Fragments arranged by size:
4935 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5749
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5714
DraI TTT'AAA
Cuts at: 3053 3053
Size: 5749
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4756 809
Size: 507 3440 1802
Fragments arranged by size:
3440 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2091 2109
Size: 54 1425 50 275 18 195 66 18
Cuts at: 2109 2472 3196 4085 5714 8
Size: 363 724 889 1629 43
Fragments arranged by size:
1629 1425 889 724 363 275 195 66
54 50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 3196 1537
Size: 275 1384 4090
Fragments arranged by size:
4090 1384 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2072 2156 2531 2855 3679 1353
Size: 719 84 375 324 824 3423
Fragments arranged by size:
3423 824 719 375 324 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4336 4710 4856 387
Size: 1064 138 2747 374 146 1280
Fragments arranged by size:
2747 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2053 2137 2931 3023 3812 4409 5196
Size: 393 421 84 794 92 789 597 787
Cuts at: 5196 5464 1239
Size: 268 1524
Fragments arranged by size:
1524 794 789 787 597 421 393 268
92 84
EcoNI CCTnn'n_nnAGG
Cuts at: 2351 2351
Size: 5749
EcoRI G'AATT_C
Cuts at: 3058 3436 3058
Size: 378 5371
FspI TGC'GCA
Cuts at: 4255 4255
Size: 5749
HaeII r_GCGC'y
Cuts at: 1678 4896 1678
Size: 3218 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4390 4422 4464 4496 1373
Size: 32 135 32 2818 32 42 32 2626
Fragments arranged by size:
2818 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3015 3093 3339 669
Size: 988 281 1077 78 246 3079
Fragments arranged by size:
3079 1077 988 281 246 78
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5749
HpaI GTT'AAC
Cuts at: 3015 3339 3015
Size: 324 5425
KpnI G_GTAC'C
Cuts at: 3074 3074
Size: 5749
MfeI C'AATT_G
Cuts at: 2294 3348 2294
Size: 1054 4695
MluI A'CGCG_T
Cuts at: 5625 5625
Size: 5749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2025 2109 4863 5047 1305
Size: 720 84 2754 184 2007
Fragments arranged by size:
2754 2007 720 184 84
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2027 2093 2111 2474 10
Size: 54 1425 343 195 66 18 363 3285
Fragments arranged by size:
3285 1425 363 343 195 66 54 18
MslI CAynn'nnrTG
Cuts at: 518 1166 2021 2057 2105 2195 2273 2309 2363
Size: 648 855 36 48 90 78 36 54
Cuts at: 2363 2588 2729 2876 2948 3707 4066 4225 518
Size: 225 141 147 72 759 359 159 2042
Fragments arranged by size:
2042 855 759 648 359 225 159 147
141 90 78 72 54 48 36 36
MspA1I CmG'CkG
Cuts at: 1606 3165 3842 4990 5235 1606
Size: 1559 677 1148 245 2120
Fragments arranged by size:
2120 1559 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2451 513
Size: 552 939 447 3811
Fragments arranged by size:
3811 939 552 447
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5749
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5749
NotI GC'GGCC_GC
Cuts at: 3196 3196
Size: 5749
NspI r_CATG'y
Cuts at: 1132 1229 2881 3107 4652 1132
Size: 97 1652 226 1545 2229
Fragments arranged by size:
2229 1652 1545 226 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5749
PciI A'CATG_T
Cuts at: 4648 4648
Size: 5749
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2359 1108
Size: 1251 4498
PfoI T'CCnGG_A
Cuts at: 2346 2346
Size: 5749
PmeI GTTT'AAAC
Cuts at: 3053 3053
Size: 5749
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3818 3850 5331 5363 3818
Size: 32 1481 32 4204
Fragments arranged by size:
4204 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2840 1613
Size: 1227 4522
PsiI TTA'TAA
Cuts at: 3319 3319
Size: 5749
PstI C_TGCA'G
Cuts at: 830 3101 830
Size: 2271 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5749
SalI G'TCGA_C
Cuts at: 1936 3091 1936
Size: 1155 4594
SbfI CC_TGCA'GG
Cuts at: 3101 3101
Size: 5749
ScaI AGT'ACT
Cuts at: 3997 5709 3997
Size: 1712 4037
SexAI A'CCwGG_T
Cuts at: 2352 2352
Size: 5749
SfcI C'TryA_G
Cuts at: 826 1045 2968 3097 4232 4913 5104 826
Size: 219 1923 129 1135 681 191 1471
Fragments arranged by size:
1923 1471 1135 681 219 191 129
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5749
SmaI CCC'GGG
Cuts at: 1872 2615 3076 1872
Size: 743 461 4545
Fragments arranged by size:
4545 743 461
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3853 4754 5016 820
Size: 197 926 1910 901 262 1553
Fragments arranged by size:
1910 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3540 493
Size: 3047 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5749
SphI G_CATG'C
Cuts at: 1132 3107 1132
Size: 1975 3774
SspI AAT'ATT
Cuts at: 5 52 3673 5
Size: 47 3621 2081
Fragments arranged by size:
3621 2081 47
StuI AGG'CCT
Cuts at: 1517 2810 1517
Size: 1293 4456
StyI C'CwwG_G
Cuts at: 513 1065 2004 2451 3188 513
Size: 552 939 447 737 3074
Fragments arranged by size:
3074 939 737 552 447
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2351 3776 3793 3946 4131 4576 325
Size: 1255 771 1425 17 153 185 445 1498
Fragments arranged by size:
1498 1425 1255 771 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2277 2697 3995
Size: 274 80 33 51 1443 300 420 1298
Cuts at: 3995 5707 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1298 420 300 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2563 2595 5709 5741 2563
Size: 32 3114 32 2571
Fragments arranged by size:
3114 2571 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2354 1316
Size: 1038 4711
XbaI T'CTAG_A
Cuts at: 3085 3085
Size: 5749
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2086 2344 2086
Size: 258 5491
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5749
XmaI C'CCGG_G
Cuts at: 1870 2613 3074 1870
Size: 743 461 4545
Fragments arranged by size:
4545 743 461
XmnI GAAnn'nnTTC
Cuts at: 3878 3878
Size: 5749
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3557 276
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI