Restriction map for
pFN21AE5492
(Circular) (Six-base) MAPSORT of: pFN21AE5492.seq Check: 1983 from: 1 to: 5740
pFN21AE5492 5740 bp
With 182 enzymes: SgfI *
January 30, 2014 17:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3550 278
Size: 53 83 186 936 2014 2468
Fragments arranged by size:
2468 2014 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3083 1937
Size: 1146 4594
Acc65I G'GTAC_C
Cuts at: 3061 3061
Size: 5740
AclI AA'CG_TT
Cuts at: 1563 3867 4240 1563
Size: 2304 373 3063
Fragments arranged by size:
3063 2304 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2874 3803 5187 5455 1632
Size: 1242 929 1384 268 1917
Fragments arranged by size:
1917 1384 1242 929 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5543
AflIII A'CryG_T
Cuts at: 1203 4639 5616 1203
Size: 3436 977 1327
Fragments arranged by size:
3436 1327 977
AgeI A'CCGG_T
Cuts at: 4571 4571
Size: 5740
AhdI GACnn_n'nnGTC
Cuts at: 4469 4469
Size: 5740
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5740
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5740
AlwNI CAG_nnn'CTG
Cuts at: 2020 3483 5055 2020
Size: 1463 1572 2705
Fragments arranged by size:
2705 1572 1463
ApaLI G'TGCA_C
Cuts at: 3797 4953 3797
Size: 1156 4584
ApoI r'AATT_y
Cuts at: 1493 2099 3049 3275 3427 5536 1493
Size: 606 950 226 152 2109 1697
Fragments arranged by size:
2109 1697 950 606 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4294 160
Size: 872 3262 1606
Fragments arranged by size:
3262 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
AvaI C'yCGr_G
Cuts at: 1870 1943 3065 1870
Size: 73 1122 4545
Fragments arranged by size:
4545 1122 73
AvrII C'CTAG_G
Cuts at: 2870 2870
Size: 5740
BamHI G'GATC_C
Cuts at: 2125 3070 3437 2125
Size: 945 367 4428
Fragments arranged by size:
4428 945 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3061 4516 618
Size: 325 217 627 1274 1455 1842
Fragments arranged by size:
1842 1455 1274 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3059 1391
Size: 1668 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2882 928
Size: 971 983 3786
Fragments arranged by size:
3786 983 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 1999 2021 2033 3931 3965 1903
Size: 34 50 12 22 12 1898 34 3678
Fragments arranged by size:
3678 1898 50 34 34 22 12 12
BciVI GTATCCnnnnn_n'
Cuts at: 2982 3632 4842 2982
Size: 650 1210 3880
Fragments arranged by size:
3880 1210 650
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5740
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2490 4351 136
Size: 107 122 71 631 605 818 1861 1525
Fragments arranged by size:
1861 1525 818 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2367 5735 2367
Size: 3368 2372
BlpI GC'TnA_GC
Cuts at: 3157 3157
Size: 5740
Bme1580I G_kGCm'C
Cuts at: 1790 3801 4957 1790
Size: 2011 1156 2573
Fragments arranged by size:
2573 2011 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5740
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3471 4429 442
Size: 416 53 83 399 24 2054 958 1753
Fragments arranged by size:
2054 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5740
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4400 1239
Size: 3161 2579
Bpu10I CC'TnA_GC
Cuts at: 2613 2613
Size: 5740
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2989 3865 4730 5028 2989
Size: 876 865 298 3701
Fragments arranged by size:
3701 876 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2799 4403 882
Size: 617 1300 1604 2219
Fragments arranged by size:
2219 1604 1300 617
BsaAI yAC'GTr
Cuts at: 493 1204 2346 3531 493
Size: 711 1142 1185 2702
Fragments arranged by size:
2702 1185 1142 711
BsaBI GATnn'nnATC
Cuts at: 5734 5734
Size: 5740
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3547 3929 4564 275
Size: 53 83 186 936 2014 382 635 1451
Fragments arranged by size:
2014 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2128 4173 4571 4845 4992 5403 2128
Size: 2045 398 274 147 411 2465
Fragments arranged by size:
2465 2045 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5515
Fragments arranged by size:
5515 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5740
BseYI C'CCAG_C
Cuts at: 4943 5439 4943
Size: 496 5244
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2557 1163
Size: 573 821 4346
Fragments arranged by size:
4346 821 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3190 3951 4979 1540
Size: 275 186 1189 761 1028 2301
Fragments arranged by size:
2301 1189 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3801 3886 4957 3801
Size: 85 1071 4584
Fragments arranged by size:
4584 1071 85
BsmI GAATG_Cn'
Cuts at: 2536 2754 3251 3344 2536
Size: 218 497 93 4932
Fragments arranged by size:
4932 497 218 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3059 3801 3886 4957 1391
Size: 399 1269 742 85 1071 2174
Fragments arranged by size:
2174 1269 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 2128 5403 2128
Size: 3275 2465
BspHI T'CATG_A
Cuts at: 2472 3629 5359 2472
Size: 1157 1730 2853
Fragments arranged by size:
2853 1730 1157
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2202 2819 3095 844
Size: 1358 617 276 3489
Fragments arranged by size:
3489 1358 617 276
BsrBI CCG'CTC
Cuts at: 1394 1823 2593 3627 1394
Size: 429 770 1034 3507
Fragments arranged by size:
3507 1034 770 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2424 4235 4409 65
Size: 1183 1176 1811 174 1396
Fragments arranged by size:
1811 1396 1183 1176 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4384 4571 1700
Size: 110 2574 187 2869
Fragments arranged by size:
2869 2574 187 110
BsrGI T'GTAC_A
Cuts at: 96 2841 96
Size: 2745 2995
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5740
BssSI C'ACGA_G
Cuts at: 3800 4812 3800
Size: 1012 4728
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2020 794
Size: 1226 4514
BstBI TT'CG_AA
Cuts at: 4544 4544
Size: 5740
BstEII G'GTnAC_C
Cuts at: 1184 2176 1184
Size: 992 4748
BstXI CCAn_nnnn'nTGG
Cuts at: 2173 2173
Size: 5740
BstYI r'GATC_y
Cuts at: 1972 2125 2367 3070 3437 3822 3839 5291 5377
Size: 153 242 703 367 385 17 1452 86
Cuts at: 5377 5389 5735 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 703 385 367 346 242 153
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5188
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5001
Fragments arranged by size:
5001 683 56
BtsI GCAGTG_nn'
Cuts at: 2889 3256 4050 4070 2889
Size: 367 794 20 4559
Fragments arranged by size:
4559 794 367 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5740
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5705
DraI TTT'AAA
Cuts at: 3044 3044
Size: 5740
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4747 809
Size: 507 3431 1802
Fragments arranged by size:
3431 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2439 3187 4076
Size: 54 1425 50 275 18 609 748 889
Cuts at: 4076 5705 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 748 609 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3187 1537
Size: 275 1375 4090
Fragments arranged by size:
4090 1375 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2600 3003 3670 1353
Size: 1247 403 667 3423
Fragments arranged by size:
3423 1247 667 403
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4327 4701 4847 387
Size: 1064 138 2738 374 146 1280
Fragments arranged by size:
2738 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2874 3803 4400 5187 5455 1239
Size: 393 1242 929 597 787 268 1524
Fragments arranged by size:
1524 1242 929 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3049 3427 3049
Size: 378 5362
FspI TGC'GCA
Cuts at: 4246 4246
Size: 5740
HaeII r_GCGC'y
Cuts at: 1678 4887 1678
Size: 3209 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2043 2075 2110 2142 2451
Size: 32 135 32 471 32 35 32 309
Cuts at: 2451 2483 2980 3012 4381 4413 4455 4487 1373
Size: 32 497 32 1369 32 42 32 2626
Fragments arranged by size:
2626 1369 497 471 309 135 42 35
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2517 3084 3330 669
Size: 988 281 579 567 246 3079
Fragments arranged by size:
3079 988 579 567 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5740
HpaI GTT'AAC
Cuts at: 3330 3330
Size: 5740
KpnI G_GTAC'C
Cuts at: 3065 3065
Size: 5740
MfeI C'AATT_G
Cuts at: 3339 3339
Size: 5740
MluI A'CGCG_T
Cuts at: 5616 5616
Size: 5740
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2647 4854 5038 1305
Size: 1342 2207 184 2007
Fragments arranged by size:
2207 2007 1342 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2441 10
Size: 54 1425 343 609 3309
Fragments arranged by size:
3309 1425 609 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2240 3698 4057 4216 518
Size: 648 843 231 1458 359 159 2042
Fragments arranged by size:
2042 1458 843 648 359 231 159
MspA1I CmG'CkG
Cuts at: 1606 2017 3156 3833 4981 5226 1606
Size: 411 1139 677 1148 245 2120
Fragments arranged by size:
2120 1148 1139 677 411 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5188
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5740
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5740
NotI GC'GGCC_GC
Cuts at: 3187 3187
Size: 5740
NspI r_CATG'y
Cuts at: 1132 1229 3098 4643 1132
Size: 97 1869 1545 2229
Fragments arranged by size:
2229 1869 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5740
PciI A'CATG_T
Cuts at: 4639 4639
Size: 5740
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5740
PmeI GTTT'AAAC
Cuts at: 3044 3044
Size: 5740
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3809 3841 5322 5354 3809
Size: 32 1481 32 4195
Fragments arranged by size:
4195 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5740
PsiI TTA'TAA
Cuts at: 3310 3310
Size: 5740
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2891 2923 2891
Size: 32 5708
PstI C_TGCA'G
Cuts at: 830 2139 2884 3092 830
Size: 1309 745 208 3478
Fragments arranged by size:
3478 1309 745 208
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5740
PvuII CAG'CTG
Cuts at: 2017 2017
Size: 5740
SalI G'TCGA_C
Cuts at: 1936 3082 1936
Size: 1146 4594
SapI GCTCTTCn'nnn_
Cuts at: 2600 2600
Size: 5740
SbfI CC_TGCA'GG
Cuts at: 3092 3092
Size: 5740
ScaI AGT'ACT
Cuts at: 3988 5700 3988
Size: 1712 4028
SfcI C'TryA_G
Cuts at: 826 1045 2135 2880 2935 3088 4223 4904 5095
Size: 219 1090 745 55 153 1135 681 191
Cuts at: 5095 826
Size: 1471
Fragments arranged by size:
1471 1135 1090 745 681 219 191 153
55
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5740
SmaI CCC'GGG
Cuts at: 1872 3067 1872
Size: 1195 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2968 3844 4745 5007 820
Size: 197 926 1025 876 901 262 1553
Fragments arranged by size:
1553 1025 926 901 876 262 197
SnaBI TAC'GTA
Cuts at: 493 3531 493
Size: 3038 2702
SpeI A'CTAG_T
Cuts at: 152 2519 152
Size: 2367 3373
SphI G_CATG'C
Cuts at: 1132 3098 1132
Size: 1966 3774
SspI AAT'ATT
Cuts at: 5 52 3664 5
Size: 47 3612 2081
Fragments arranged by size:
3612 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5740
StyI C'CwwG_G
Cuts at: 513 1065 2870 3179 513
Size: 552 1805 309 3074
Fragments arranged by size:
3074 1805 552 309
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2378 3767 3784 3937 4122 4567 325
Size: 1255 798 1389 17 153 185 445 1498
Fragments arranged by size:
1498 1389 1255 798 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2169 2244 2841
Size: 274 80 33 51 1443 192 75 597
Cuts at: 2841 3986 5698 96
Size: 1145 1712 138
Fragments arranged by size:
1712 1443 1145 597 274 192 138 80
75 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5700 5732 5700
Size: 32 5708
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5740
XbaI T'CTAG_A
Cuts at: 3034 3076 3034
Size: 42 5698
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5740
XmaI C'CCGG_G
Cuts at: 1870 3065 1870
Size: 1195 4545
XmnI GAAnn'nnTTC
Cuts at: 3869 3869
Size: 5740
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3548 276
Size: 53 83 186 936 2014 2468
Fragments arranged by size:
2468 2014 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI