Restriction map for
pFN21AE5491
(Circular) (Six-base) MAPSORT of: pFN21AE5491.seq Check: 7763 from: 1 to: 5737
pFN21AE5491 5737 bp
With 182 enzymes: SgfI *
January 30, 2014 17:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3547 278
Size: 53 83 186 936 2011 2468
Fragments arranged by size:
2468 2011 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3080 1937
Size: 1143 4594
Acc65I G'GTAC_C
Cuts at: 3058 3058
Size: 5737
AclI AA'CG_TT
Cuts at: 1563 3864 4237 1563
Size: 2301 373 3063
Fragments arranged by size:
3063 2301 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2067 2439 2975 3800 5184 5452 1632
Size: 435 372 536 825 1384 268 1917
Fragments arranged by size:
1917 1384 825 536 435 372 268
AflII C'TTAA_G
Cuts at: 820 1017 2762 820
Size: 197 1745 3795
Fragments arranged by size:
3795 1745 197
AflIII A'CryG_T
Cuts at: 1203 4636 5613 1203
Size: 3433 977 1327
Fragments arranged by size:
3433 1327 977
AgeI A'CCGG_T
Cuts at: 4568 4568
Size: 5737
AhdI GACnn_n'nnGTC
Cuts at: 2603 4466 2603
Size: 1863 3874
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5737
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5737
AlwNI CAG_nnn'CTG
Cuts at: 2251 3480 5052 2251
Size: 1229 1572 2936
Fragments arranged by size:
2936 1572 1229
ApaLI G'TGCA_C
Cuts at: 3794 4950 3794
Size: 1156 4581
ApoI r'AATT_y
Cuts at: 1493 2030 2120 3046 3272 3424 5533 1493
Size: 537 90 926 226 152 2109 1697
Fragments arranged by size:
2109 1697 926 537 226 152 90
AseI AT'TA_AT
Cuts at: 160 1032 4291 160
Size: 872 3259 1606
Fragments arranged by size:
3259 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5737
AvaI C'yCGr_G
Cuts at: 1870 1943 3062 1870
Size: 73 1119 4545
Fragments arranged by size:
4545 1119 73
BamHI G'GATC_C
Cuts at: 3067 3434 3067
Size: 367 5370
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3058 4513 618
Size: 325 217 627 1271 1455 1842
Fragments arranged by size:
1842 1455 1271 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2139 3056 1391
Size: 748 917 4072
Fragments arranged by size:
4072 917 748
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4766
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3928 3962 1903
Size: 34 1991 34 3678
Fragments arranged by size:
3678 1991 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2643 3629 4839 2643
Size: 986 1210 3541
Fragments arranged by size:
3541 1210 986
BclI T'GATC_A
Cuts at: 1548 2487 1548
Size: 939 4798
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4348 136
Size: 107 122 71 631 605 2676 1525
Fragments arranged by size:
2676 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5732 5732
Size: 5737
BlpI GC'TnA_GC
Cuts at: 3010 3154 3010
Size: 144 5593
Bme1580I G_kGCm'C
Cuts at: 1790 3798 4954 1790
Size: 2008 1156 2573
Fragments arranged by size:
2573 2008 1156
BmgBI CAC'GTC
Cuts at: 1318 2818 1318
Size: 1500 4237
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2682 3468 4426
Size: 416 53 83 399 24 1265 786 958
Cuts at: 4426 442
Size: 1753
Fragments arranged by size:
1753 1265 958 786 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5737
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4397 1239
Size: 3158 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3862 4727 5025 3862
Size: 865 298 4574
Fragments arranged by size:
4574 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4400 882
Size: 617 2901 2219
Fragments arranged by size:
2901 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3528 493
Size: 711 2324 2702
Fragments arranged by size:
2702 2324 711
BsaBI GATnn'nnATC
Cuts at: 2631 5731 2631
Size: 3100 2637
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3544 3926 4561 275
Size: 53 83 186 936 2011 382 635 1451
Fragments arranged by size:
2011 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4170 4568 4842 4989 5400 4170
Size: 398 274 147 411 4507
Fragments arranged by size:
4507 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5512
Fragments arranged by size:
5512 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2712 1186
Size: 1526 4211
BseYI C'CCAG_C
Cuts at: 3014 4940 5436 3014
Size: 1926 496 3315
Fragments arranged by size:
3315 1926 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5164
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3187 3948 4976 1540
Size: 275 186 1186 761 1028 2301
Fragments arranged by size:
2301 1186 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3798 3883 4954 3798
Size: 85 1071 4581
Fragments arranged by size:
4581 1071 85
BsmI GAATG_Cn'
Cuts at: 2965 3248 3341 2965
Size: 283 93 5361
Fragments arranged by size:
5361 283 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2139 3056 3798 3883 4954 1391
Size: 399 349 917 742 85 1071 2174
Fragments arranged by size:
2174 1071 917 742 399 349 85
BspEI T'CCGG_A
Cuts at: 5400 5400
Size: 5737
BspHI T'CATG_A
Cuts at: 2479 3626 5356 2479
Size: 1147 1730 2860
Fragments arranged by size:
2860 1730 1147
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2223 3092 844
Size: 1379 869 3489
Fragments arranged by size:
3489 1379 869
BsrBI CCG'CTC
Cuts at: 1394 1823 3624 1394
Size: 429 1801 3507
Fragments arranged by size:
3507 1801 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4232 4406 65
Size: 1183 2984 174 1396
Fragments arranged by size:
2984 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4381 4568 1700
Size: 110 2571 187 2869
Fragments arranged by size:
2869 2571 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5737
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5737
BssSI C'ACGA_G
Cuts at: 3797 4809 3797
Size: 1012 4725
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5737
BstBI TT'CG_AA
Cuts at: 4541 4541
Size: 5737
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5737
BstYI r'GATC_y
Cuts at: 1972 3067 3434 3819 3836 5288 5374 5386 5732
Size: 1095 367 385 17 1452 86 12 346
Cuts at: 5732 1972
Size: 1977
Fragments arranged by size:
1977 1452 1095 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2151 2151
Size: 5737
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4246
Fragments arranged by size:
4246 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4998
Fragments arranged by size:
4998 683 56
BtsI GCAGTG_nn'
Cuts at: 3253 4047 4067 3253
Size: 794 20 4923
Fragments arranged by size:
4923 794 20
ClaI AT'CG_AT
Cuts at: 1554 2452 1554
Size: 898 4839
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5702
DraI TTT'AAA
Cuts at: 3041 3041
Size: 5737
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2070 4744 809
Size: 507 754 2674 1802
Fragments arranged by size:
2674 1802 754 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2238 2460 3184
Size: 54 1425 50 275 18 408 222 724
Cuts at: 3184 4073 5702 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 724 408 275 222 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3184 1537
Size: 275 1372 4090
Fragments arranged by size:
4090 1372 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2481 3667 1353
Size: 1128 1186 3423
Fragments arranged by size:
3423 1186 1128
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4324 4698 4844 387
Size: 1064 138 2735 374 146 1280
Fragments arranged by size:
2735 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2067 2439 2975 3800 4397 5184 5452
Size: 393 435 372 536 825 597 787 268
Cuts at: 5452 1239
Size: 1524
Fragments arranged by size:
1524 825 787 597 536 435 393 372
268
EcoRI G'AATT_C
Cuts at: 3046 3424 3046
Size: 378 5359
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2444 2444
Size: 5737
FspI TGC'GCA
Cuts at: 4243 4243
Size: 5737
HaeII r_GCGC'y
Cuts at: 1678 4884 1678
Size: 3206 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2008 2040 2330 2362 2589
Size: 32 135 32 436 32 290 32 227
Cuts at: 2589 2621 4378 4410 4452 4484 1373
Size: 32 1757 32 42 32 2626
Fragments arranged by size:
2626 1757 436 290 227 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2338 3081 3327 669
Size: 988 281 400 743 246 3079
Fragments arranged by size:
3079 988 743 400 281 246
HindIII A'AGCT_T
Cuts at: 748 2793 3007 748
Size: 2045 214 3478
Fragments arranged by size:
3478 2045 214
HpaI GTT'AAC
Cuts at: 3327 3327
Size: 5737
KpnI G_GTAC'C
Cuts at: 3062 3062
Size: 5737
MfeI C'AATT_G
Cuts at: 3336 3336
Size: 5737
MluI A'CGCG_T
Cuts at: 5613 5613
Size: 5737
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2086 2099 4851 5035 1305
Size: 781 13 2752 184 2007
Fragments arranged by size:
2752 2007 781 184 13
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2240 2462 10
Size: 54 1425 343 408 222 3285
Fragments arranged by size:
3285 1425 408 343 222 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3695 4054 4213 518
Size: 648 2529 359 159 2042
Fragments arranged by size:
2529 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3153 3830 4978 5223 1606
Size: 1547 677 1148 245 2120
Fragments arranged by size:
2120 1547 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4246
Fragments arranged by size:
4246 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5737
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5737
NotI GC'GGCC_GC
Cuts at: 3184 3184
Size: 5737
NspI r_CATG'y
Cuts at: 1132 1229 3095 4640 1132
Size: 97 1866 1545 2229
Fragments arranged by size:
2229 1866 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2251 1348
Size: 903 4834
PciI A'CATG_T
Cuts at: 4636 4636
Size: 5737
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2251 1108
Size: 1143 4594
PmeI GTTT'AAAC
Cuts at: 3041 3041
Size: 5737
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2496 2528 3806 3838 5319 5351 2496
Size: 32 1278 32 1481 32 2882
Fragments arranged by size:
2882 1481 1278 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5737
PsiI TTA'TAA
Cuts at: 2921 3307 2921
Size: 386 5351
PstI C_TGCA'G
Cuts at: 830 3089 830
Size: 2259 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5737
SalI G'TCGA_C
Cuts at: 1936 3079 1936
Size: 1143 4594
SbfI CC_TGCA'GG
Cuts at: 3089 3089
Size: 5737
ScaI AGT'ACT
Cuts at: 3985 5697 3985
Size: 1712 4025
SfcI C'TryA_G
Cuts at: 826 1045 2904 3085 4220 4901 5092 826
Size: 219 1859 181 1135 681 191 1471
Fragments arranged by size:
1859 1471 1135 681 219 191 181
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5737
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5737
SmaI CCC'GGG
Cuts at: 1872 3064 1872
Size: 1192 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2762 3841 4742 5004 820
Size: 197 926 819 1079 901 262 1553
Fragments arranged by size:
1553 1079 926 901 819 262 197
SnaBI TAC'GTA
Cuts at: 493 3528 493
Size: 3035 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5737
SphI G_CATG'C
Cuts at: 1132 3095 1132
Size: 1963 3774
SspI AAT'ATT
Cuts at: 5 52 3661 5
Size: 47 3609 2081
Fragments arranged by size:
3609 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5737
StyI C'CwwG_G
Cuts at: 513 1065 2004 3176 513
Size: 552 939 1172 3074
Fragments arranged by size:
3074 1172 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3764 3781 3934 4119 4564 325
Size: 1255 2184 17 153 185 445 1498
Fragments arranged by size:
2184 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2265 3983 5695
Size: 274 80 33 51 1443 288 1718 1712
Cuts at: 5695 96
Size: 138
Fragments arranged by size:
1718 1712 1443 288 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5697 5729 5697
Size: 32 5705
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5737
XbaI T'CTAG_A
Cuts at: 3073 3073
Size: 5737
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2248 2248
Size: 5737
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5737
XmaI C'CCGG_G
Cuts at: 1870 3062 1870
Size: 1192 4545
XmnI GAAnn'nnTTC
Cuts at: 2424 3866 2424
Size: 1442 4295
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3545 276
Size: 53 83 186 936 2011 2468
Fragments arranged by size:
2468 2011 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI